Among the Orchidaceae is found the Cattleya, an important genus for commercial hybrid production. However, many Cattleya species are facing extinction due to over-collection and habitat loss. Cattleya elongata is a species endemic to Bahia; these plants have attractive brownish-purple tepals and an attractive purple labellum. This species has been submitted to collections; however, it is not easy to grow in vitro, and as such, it is rarely cultured. This work aimed to determine the best conditions for plant production and acclimatization to allow either commercialization or reintroduction. Germinated seeds were sown in three different culture media, Knudson C (Kc), Murashige and Skooge (½-concentration MS) and Vacin and Went (VW) media, and grown for 270 days. The length of the roots and shoots and their fresh matter were determined, and survival in culture media and acclimatization were also scored. The in vitro development of C. elongata was best in the ½-concentration MS and VW media. The best choice for C. elongata propagation was the ½-concentration MS, as they were superior either for in vitro development or for acclimatization.