In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm.

Edy Setiti Wida Utami,Sucipto Hariyanto

doi:10.1155/2019/8105138

Abstract

Phalaenopsis amboinensis, an epiphytic orchid, has a great potential for commercial exploitation in the cut-flower industry. It is difficult to propagate vegetatively as it naturally grows slowly. Therefore, there is a need to improve the propagation methods to avoid endangering its natural populations. The objective of this study was to identify the best medium and organic supplements for seed germination and plantlets development of P. amboinensis. Seeds from 4-month-old hand-pollinated orchids were sown on different strengths of MS and VW culture media: Murashige and Skoog (MS), 1/2 MS, Vacin and Went (VW), and 1/2 VW. Optimum seed germination, i.e., 90.7%, was achieved on VW medium. VW medium was suitable for seedling formation and allowed 51.4% of seedling development from protocorm within 10 weeks of culture. When 15% (v/v) coconut water was added together with banana homogenate (10 g·L−1) to the VW medium, the plantlets grew to the highest length and had the highest dry weight (62.1 mm and 15.5 g, respectively). The roots and leaves of the plantlets grew vigorously in this medium. Plants regenerated via in vitro seed germination processes were successfully acclimatized in greenhouse conditions, and the survival rate was more than 85%.

Highlights

Phalaenopsis, the moth orchid, has been recognized as one of the most beautiful flowers in the world [1]. is genus has economic value for pot plant and cut-flower production
Phalaenopsis amboinensis is a native species from Indonesia which has a great potential for commercial exploitation in cut-flower industry and is a parent for orchid breeding. e product of plant breeding of P. amboinensis with P. amabilis could potentially produce a greater flower and prettier color than both parents. e plant breeding of P. amboinensis with P. violacea produced the hybrid P
We evaluated the effects of different strengths of Murashige and Skoog (MS) and Vacin and Went (VW) media on seed germination and supplementation of coconut water with various concentrations of banana homogenate on the plantlet development of P. amboinensis. e reproducible protocol for the production of seedlings from seeds by in vitro culture of this species has been established

Summary

Edy Setiti Wida Utami and Sucipto Hariyanto

It is difficult to propagate vegetatively as it naturally grows slowly. Erefore, there is a need to improve the propagation methods to avoid endangering its natural populations. E objective of this study was to identify the best medium and organic supplements for seed germination and plantlets development of P. amboinensis. Seeds from 4-month-old hand-pollinated orchids were sown on different strengths of MS and VW culture media: Murashige and Skoog (MS), 1/2 MS, Vacin and Went (VW), and 1/2 VW. I.e., 90.7%, was achieved on VW medium. E roots and leaves of the plantlets grew vigorously in this medium. Plants regenerated via in vitro seed germination processes were successfully acclimatized in greenhouse conditions, and the survival rate was more than 85%

Introduction

Materials and Methods

Results and Discussion

No growth of embryo occurs and testa was found intact