V3 Growth Stage Research Articles

Application technology for the entomopathogenic nematodes Heterorhabditis indica and Steinernema sp. (Rhabditida: Heterorhabditidae and Steinernematidae) to control Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) in corn

The effects of different application technologies were evaluated on the concentration, viability, and efficiency of infective juveniles of the nematodes Heterorhabditis indica Poinar, Karunakar & David and Steinernema sp. (IBCB-n6) to control Spodoptera frugiperda Smith on corn plants. Two hundred and eighty infective juveniles of Steinernema sp. were required to kill 100% third-instar fall armyworms in petri dishes, as compared to 400 infective juveniles of the H. indica nematode to obtain 75% fall armyworm control. It is possible to spray entomopathogenic nematodes without significant loss in their concentration and viability, with equipment that produces electrical charges to the spraying mix, and with those using hydraulic and rotary nozzle tips. The concentrations of infective juveniles of H. indica and Steinernema sp. nematodes were reduced by 28% and 53%, respectively, when hydraulic spraying nozzles that require 100-mesh filtrating elements were used. Tensoactive agents of the organosilicone and ethoxylate groups did not affect the viability of infective juveniles of Steinernema sp. juveniles. Spraying corn plants (V6 growth stage) with up to 288 million infective juveniles of Steinernema sp. per hectare, diluted in the spraying mix up to 800 L ha-1, with 0.01% ethoxylate tensoactive agent, or at the same volume followed by artificial rain (6 mm water depth) was not sufficient to control S. frugiperda in a controlled environment.

Competitiveness with Weeds of Soybean Cultivars with Different Maturity and Canopy Width Characteristics

Field studies were conducted in 2005 at five locations throughout Illinois to determine the effect of maturity date and canopy width characteristics of selected glyphosate-resistant soybean cultivars on competitive ability against weeds. Natural weed populations at all sites were allowed to compete with the soybean cultivars until the V1, V3, V5, or V7 growth stage and then removed with an application of glyphosate. Light-interception readings, weed control ratings, and soybean yield were all measured to compare cultivars. Cultivars of later maturities tended to withstand early season weed competition better and attained higher yields when weeds were removed at later timings than those of earlier maturity. Although canopy width differed moderately among cultivars in some cases, canopy width had no effect on the ability of soybeans to compete with weeds. Thus, choosing soybean cultivars of later maturity might provide more flexibility in weed management and might reduce losses due to weeds, but that benefit needs to be balanced with yield potential.

Heterologous Hybridization of Cotton Microarrays with Velvetleaf (Abutilon theophrasti) Reveals Physiological Responses Due to Corn Competition

Microarray analysis was used to identify changes in gene expression in velvetleaf that result from competition with corn. The plants were grown in field plots under adequate N (addition of 220 kg N ha−1) to minimize stress and sampled at the V6 growth stage of corn (late June). Leaf area, dry weight, and N and P concentration were similar in velvetleaf plants grown alone or with corn. Competition, however, did influence velvetleaf gene expression. Genes involved in carbon utilization, photosynthesis, red light signaling, and cell division were preferentially expressed when velvetleaf was grown in competition with corn. A less clear picture of the physiological impact of growth in monoculture was provided by the data. However, several genes involved in secondary metabolism and a gene preferentially expressed in response to phosphate availability were induced. No differences were observed in genes responsive to water stress or sequestering/transporting micronutrients.

Effect of Tillage and Nitrogen Rate on Corn Yield and Nitrogen and Phosphorus Uptake in a Corn‐Soybean Rotation

Understanding tillage, N, and P interactions can contribute to improved N and P utilization and crop response. This study examined the interaction effects of tillage and N rate of two N sources on N and P uptake by corn (Zea mays L.). The study was conducted on Kenyon loam (fine loamy, mixed, mesic Typic Hapludolls) soil at the Iowa State University Northeast Research and Demonstration Farm near Nashua, IA. A randomized complete block design with a split‐plot arrangement in three replications was used for the two separate N source experiments. The tillage systems consist of no‐tillage (NT), strip‐tillage (ST), and chisel plow (CP) as main plots. Within each tillage four N rates (0, 85, 170, and 250 kg N ha−1) were assigned as subplots for each N source of manure (total N) and fertilizer (anhydrous ammonia) in a corn‐soybean [Glycine max (L.) Merr.] rotation. Tillage and increase in N rate beyond 85 kg ha−1 had no effect on corn grain yield with both N sources. Tillage × N rate had a significant effect on plant N and P uptake, especially at early growth stages with both N sources. Recovery percentage of applied N across all tillage systems and N rates was 40% and 27% for manure and fertilizer sources, respectively, at the 12th‐leaf growth stage of corn (V12). Plant N and P uptake at V12 growth stage was 44% and 37%, respectively, across tillage systems, N sources, and N rates. The findings indicate that the N rate and seasonal variability have more influence on plant N and P uptake than does the tillage system.

Common Waterhemp Growth and Fecundity as Influenced by Emergence Date and Competing Crop

Common waterhemp (Amarathus rudis Sauer) is a frequent weed in glyphosate‐resistant (GR) crops in the midwestern USA due, in part, to the delayed emergence of its seedlings. Variable waterhemp emergence was simulated by transplanting seedlings into both corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] and bare plot areas at differing crop growth stages during two growing seasons in western Minnesota. Growth and fecundity were measured. As expected, late planted weeds produced little dry matter and few seeds, and competition from corn or soybean reduced waterhemp dry weight and fecundity by ≥ 90% compared with isolated plants. Interestingly, common waterhemp was affected differently by crop and transplanting date. Common waterhemp grown with corn was always shaded by the crop canopy but produced seeds even when transplanted as late as the V10 growth stage. In soybean, weeds transplanted before the V4 growth stage were taller than soybean and produced more seeds than those transplanted into corn at a comparable growth stage; however, those transplanted after V5 produced no seeds. Consequently, control of late‐emerging common waterhemp plants in soybean may not be needed, whereas control of late‐emerging plants in corn may be justified because of relatively high levels of seed production.

Influence of Nitrogen Application Timing on Low Density Giant Ragweed (Ambrosia Trifida) Interference in Corn

Field experiments were conducted to evaluate the influence of three nitrogen fertilizer application timings on corn and giant ragweed dry weight, corn and giant ragweed nitrogen accumulation, and corn grain yield. By the V8 growth stage, corn plants in treatments which included nitrogen at planting accumulated up to 27% more dry weight and 26% more leaf area than corn in sidedress-only treatments that had not yet received any fertilizer nitrogen. Nitrogen fertilizer application timing did not influence early-season giant ragweed dry weight, but did influence N accumulation. Giant ragweed at 0.5 plants/m2 accumulated up to 16 kg N/ha in 40-cm-tall plants by the V8 corn growth stage and up to 104 kg N/ha when allowed to remain in corn until the end of the growing season. At this density of giant ragweed, corn yields were not reduced if removed by the V8 corn growth stage. However, season-long interference reduced corn yields by up to 19% regardless of nitrogen application timing. Early-season giant ragweed growth was less responsive to nitrogen application timing than corn, suggesting that nitrogen management programs should not be altered significantly to influence giant ragweed competitiveness in corn. However, giant ragweed should be removed in a timely manner to minimize nitrogen accumulation in nitrogen-limiting corn production situations.Nomenclature: Giant ragweed, Ambrosia trifida L. AMBTR, corn, Zea mays L. ‘DKC60-09RR2/YGCB’ and ‘DKC61-45RR/YGCB’

Maize response to nitrogen fertilization timing in two tillage systems in a soil with high organic matter content

No-tillage systems, associated to black oat as preceding cover crop, have been increasingly adopted. This has motivated anticipated maize nitrogen fertilization, transferring it from the side-dress system at the stage when plants have five to six expanded leaves to when the preceding cover crop is eliminated or to maize sowing. This study was conducted to evaluate the effects of soil tillage system and timing of N fertilization on maize grain yield and agronomic efficiency of N applied to a soil with high organic matter content. A three-year field experiment was conducted in Lages, state of Santa Catarina, from 1999 onwards. Treatments were set up in a split plot arrangement. Two soil tillage systems were tested in the main plots: conventional tillage (CT) and no-tillage (NT). Six N management systems were assessed in the split-plots: S1 - control, without N application; S2 - all N (100 kg ha-1) applied at oat desiccation; S3 - all N applied at maize sowing; S4 - all N side-dressed when maize had five expanded leaves (V5 growth stage); S5 - 1/3 of N rate applied at maize sowing and 2/3 at V5; S6 - 2/3 of nitrogen rate applied at maize sowing and 1/3 at V5. Maize response to the time and form of splitting N was not affected by the soil tillage system. Grain yield ranged from 6.0 to 11.8 t ha-1. The anticipation of N application (S2 and S3) decreased grain yield in two of three years. In the rainiest early spring season (2000/2001) of the experiment, S4 promoted an yield advantage of 2.2 t ha-1 over S2 and S3. Application of total N rate before or at sowing decreased the number of kernels produced per ear in 2000/2001 and 2001/2002 and the number of ears produced per area in 2001/2002, resulting in reduced grain yield. The agronomic efficiency of applied N (kg grain increase/kg of N applied) ranged from 13.9 to 38.8 and was always higher in the S4 than in the S2 and S3 N systems. Short-term N immobilization did not reduce grain yield when no N was applied before or at maize sowing in a soil with high organic matter content, regardless of the soil tillage system.

Response of Soybean Cultivars to Bean pod mottle virus Infection.

Bean pod mottle virus (BPMV) has become increasingly common in soybean throughout the north-central region of the United States. Yield loss assessments on southern soybean germplasm have reported reductions ranging from 3 to 52%. Currently, no soybean cultivars have been identified with resistance to BPMV. The objective of this study was to determine the impact of BPMV infection on soybean cultivars representing a broad range of northern soybean germ-plasm by comparing inoculated and noninoculated soybean plants in paired row studies. In all, 30 and 24 cultivars were evaluated in Nebraska (NE) in which soybean plants were inoculated at the V3 to V4 growth stage. Eleven cultivars from public and breeding lines were inoculated at the VC and R5 to R6 growth stages in Ohio (OH). Disease severity, yield, and percent seed coat mottling were assessed at both locations, whereas protein and oil content also were assessed at NE. Yield and percent seed coat mottling was significantly reduced following inoculation at the VC (OH) and V3 to V4 (NE) growth stages. In addition, seed oil and protein composition were impacted in 1 of the 2 years of the study. This study demonstrates that substantial yield losses can occur in soybean due to BPMV infection. In addition, protein and oil may be affected depending on the environment during the production season.

Determination of Optimum Rate and Growth Stage for Foliar‐Applied Phosphorus in Corn

Foliar applications of fertilizer phosphorus (P) could improve use efficiency by minimizing soil applications. Nine experiments were conducted in 2002 and 2003 to determine foliar P rates and appropriate growth stages for application. Treatments comprised of 10 factorial combinations of three foliar P application timings and four rates of foliar P. Foliar application times were V4 (collar of fourth leaf visible), V8 (collar of eighth leaf visible), and VT (last branch of the tassel completely visible but silks not yet emerged) corn growth stages. Foliar P rates were 0, 2, 4, and 8 kg ha−1. Foliar P applied at the VT growth stage improved grain and forage P concentration, which was reflected in increased grain yield in some of the experiments. A foliar P rate of 8 kg ha−1 improved yield to some extent and forage and grain P concentration more than the smaller rates. The results suggest that foliar P could be used as an efficient P‐management tool in corn when applied at the appropriate growth stage and rate.

Response of Corn to Banded Zinc Sulfate Fertilizer in Fields with Variable Soil pH

There are soil series that contain free calcium carbonate (CaCO3) within fields where the surrounding pHs of the soil series are slightly acid. The objective was to study corn (Zea mays L.) grain yield response to zinc (Zn) fertilizer on different soil series within fields. The study was conducted at 12 sites between 1998 and 2000. Treatments were 0 and 5.6 kg Zn ha−1 applied in bands to field‐length strips of corn and replicated four times. Multiple soil series were identified at each site, and treatment pairs were located within them. Grain yields, whole plant (V6 to V10 growth stage), and grain Zn content and uptake were measured. The most common result was no response to Zn application. However, significant yield decreases occurred on some soils as a result of Zn application in two situations: on soils with high soil‐test Zn or on soils with low soil‐test Zn and high pH.

Expression of Variability in Corn as Influenced by Growth Stage Using Optical Sensor Measurements

Improving crop management inputs with remote sensing devices is an emerging technology. This study documented the progression of the normalized difference vegetative index (NDVI) during the life cycle of corn (Zea mays L.), evaluated the spatial variability of corn growth in terms of the CV (calculated from NDVI readings), and documented the relationships between NDVI, CV (calculated from NDVI), and grain and biomass yields and plant density. Four rows, 30 m in length, from two locations during 2 yr were randomly selected for this study. An optical sensor was used to collect NDVI readings at multiple growth stages during the life cycle of corn. The NDVI increased with progression of vegetative growth stages until V10, where a plateau was encountered, followed by a decline in NDVI after the VT growth stage. Coefficient of variation data from the NDVI readings revealed two dominant peaks during the life cycle of corn, one between the V6 and V8 growth stages and the second during the late reproductive growth stages. The CV data illustrated that the greatest variation expressed by corn during the vegetative growth stages was between the V6 and V8 growth stages. The highest correlation of NDVI with corn grain yield was found at the V7 to V9 growth stages; likewise, CV and plant density were also more highly correlated from V7 to V9. The CV from NDVI readings was highly correlated with grain and biomass yields at all growth stages.

By‐Plant Prediction of Corn Forage Biomass and Nitrogen Uptake at Various Growth Stages Using Remote Sensing and Plant Height

As research intensifies on developing precision agricultural practices for corn (Zea mays L.) production, an important component will be to identify the scale at which these practices should be implemented. We hypothesized that optical sensing can be used to measure individual corn plant biomass and N uptake. A 3‐yr study was conducted at three locations in Oklahoma. Optical sensor readings of normalized difference vegetation index (NDVI) and plant height measurements were collected on individual corn plants at various growth stages ranging from V8 (collar of eighth leaf unfolded) to VT (last branch of the tassel is completely visible) and correlated with individual plant biomass, forage yield per unit area occupied by the plant, and N uptake of that plant. Individual plant height measurement, collected before reproductive growth, was a good predictor of plant biomass across the six site years of the study (r2 = 0.81). The index of NDVI × plant height provided the highest correlation with by‐plant forage yield on an area basis. Optical sensor and plant height measurements collected at the V8 to V10 (collar of 10th leaf unfolded) growth stage can distinguish individual plants and provide information as to their biomass accumulation and N uptake. This research demonstrates that by‐plant information can be collected and used to direct high resolution N applications. The index, NDVI × plant height, may be used to refine midseason fertilizer N rates based on expected N removal and by‐plant measurements at or before V10.

Gene expression analysis in soybean in response to the causal agent of Asian soybean rust (Phakopsora pachyrhizi Sydow) in an early growth stage

Asian soybean rust (ASR) caused by Phakopsora pachyrhizi Sydow is a potentially devastating disease posing a serious threat to the soybean industry. Understanding plant host response at the molecular level is certainly important for control of the disease. The main objective of this study was to perform a transcriptome profiling of P. pachyrhizi-exposed young soybean plants (V2 growth stage) using whole genome Affymetrix microarrays of soybean. Three-week-old soybean cv. 5601 T plants at the V2 growth stage were inoculated with P. pachyrhizi, and leaf samples were collected 72 h post inoculation with subsequent microarray analysis performed. A total of 112 genes were found to be differentially expressed from P. pachyrhizi exposure, of which 46 were upregulated, and 66 were downregulated. Most of the differentially expressed genes were general defense and stress-related genes, and 34 of these were unknown. Confirmational real-time reverse transcription-polymerase chain reaction was performed on a subset of 5 out of 112 differentially expressed genes. These results were congruent with the microarray analysis. Our results indicated that low and nonspecific innate response to the pathogen may account for the failure to develop rust resistance in the soybean variety studied. To our knowledge, this is the first microarray analysis of soybean in response to ASR.

Shikimic Acid Accumulation in Field-Grown Corn (Zea mays) Following Simulated Glyphosate Drift

Field studies were conducted in 2001 through 2003 to determine if shikimic acid accumulation could be used to accurately predict yield reductions in field corn exposed to sublethal rates of glyphosate. Glyphosate (0-0.32 kg ae/ha) was applied to corn at the V6 to V8 growth stage. Corn whorls were randomly collected up to 14 days after application (DAA), and shikimic acid accumulation in the whorls was determined using HPLC-UV. Maximum shikimic acid accumulation occurred 3-7 DAA in corn receiving 0.16 and 0.32 kg/ha. Shikimic acid accumulation 3, 5, and 7 DAA did correlate (r = 0.80-0.86) to yield losses from a sublethal application of glyphosate. Shikimic acid accumulation 3, 5, and 7 DAA was better correlated to visual injury at 14 DAA than to yield reductions. Visual injury ratings 14 DAA were a slightly better indicator of potential yield losses (r = 0.93) than shikimic acid accumulation in field-grown corn whorls (r = 0.8-0.86).

First Report of Soybean Rust Caused by Phakopsora pachyrhizi on Phaseolus spp. in Argentina.

Asian soybean rust (ASR), caused by Phakopsora pachyrhizi Syd. & P. Syd., has been reported in Argentina on soybean (Glycine max) and kudzu (Pueraria lobata and Pueraria javanica) since the 2002 growing season (1-4). On 29 May 2006, plants of Phaseolus spp. were found to have tan ASR-like rust lesions on leaves at eight different field plots located in the northwestern province of Salta, Argentina. Growth stages of infected bean plants within plots were between pod setting and physiological maturity. Diagnosis of ASR on bean leaves was performed with a stereoscopic microscope to view rust pustules, and suspected uredinia of P. pachyrhizi were observed, furthermore, typical ASR urediniospores also were also observed at ×400. ELISA and PCR methods gave positive results for ASR. Rust spores from these plants were used to inoculate soybean plants at the V3 growth stage with rust spores from field bean plants produced under greenhouse conditions. Typical ASR tan pustules developed within 21 days of inoculation. Bean rust caused by Uromyces phaseoli also was seen in some of the bean plots but was easily differentiated from ASR because the uredinia were much darker and affected the upper leaves, while the ASR uredinia were lighter and spread from the lower leaves to the upper leaves. This finding is of significance in Argentina because bean is an important crop grown in the northwestern region of the country and is planted approximately 2 months after soybean planting. Given this planting time difference, bean may provide an overwintering host for the survival of ASR spores, thereby providing a green bridge for infection of soybean plants during the following growing season.

Pre‐Sidedress Nitrate Test and Other Crop‐Based Indicators for Fresh Market and Processing Sweet Corn

Commercial sweet corn (Zea mays L.) production requires significant quantities of fertilizer N, leading to inefficient N use and negative environmental impact. A field experiment was conducted for 4 yr (2001–2004) in Ottawa, Canada, to assess and compare presidedress soil nitrate test (PSNT) with some crop‐based measurements (canopy reflectance, leaf chlorophyll and plant total N) for improved N management. A fresh market sweet corn (FMSC, hybrid ‘Temptation’) grown from 2001 to 2003, and a processing sweet corn (PSC, hybrid ‘Hollywood’) from 2002 to 2004, both received five fertilizer N rates (0, 50, 100, 150, and 200 kg N ha−1). Soil samples taken from the V4 to V8 growth stages were analyzed for NO3−–N. Leaf chlorophyll content (SPAD) and canopy reflectance were also measured for FMSC at the same time. All N treatments affected the number of marketable ears, kernel dry weight and total biomass production. However, in most cases, there was no difference between N treatments from 100 to 200 kg ha−1. The PSNT NO3−–N increased linearly with the fertilizer N rates, and there were significant positive correlations between PSNT at V4 to V6 and the number of marketable ears. It was evident that PSNT, plant N concentration at V6, SPAD and canopy reflectance all differentiated sweet corn N response similarly, and they were highly correlated with one another. We concluded that PSNT at V4 to V6 was effective in predicting sweet corn N requirement in this cool and short‐growing region.

Aggressiveness between genetic groups I and II of isolates of Cercospora zeae-maydis

For many years, the gray leaf spot disease (GLS) caused by the fungus Cercospora zeae-maydis Tehon & Daniels, was not considered an important pathogen of maize (Zea mays, L.) in Brazil. However, the recent adoption of agronomical practices such as no-tillage and cultivation under central pivot irrigation systems increased the incidence and severity to the extent that GLS is now one of the most important diseases of maize. Isolates of C. zeae-maydis can be distinguished by two genetic groups (I and II) based on AFLP markers and on polymorphisms of the ITS and 5.8S rDNA regions. Until now, however, the biological implications of this distinction remain unclear. This study investigated whether isolates from the two genetic groups differ in aggressiveness towards maize. For this, symptoms of a susceptible hybrid were evaluated under greenhouse conditions with 9 and 11 isolates of C. zeae-maydis from groups I and II, respectively. Plants in the V3 growth stage were inoculated by placing sorghum seeds colonized with the pathogen in the leaf whorl and symptoms were evaluated with a visual rating scale 30 days later. On average, isolates of genetic group II were more aggressive than those of group I, with mean disease scores of 3.1 and 2.3, respectively. Differences were also observed between experiments, which suggested that group I and II might also differ in their fitness under different environments. This is the first report on differences in aggressiveness between the two genetic groups of C. zeae-maydis.

Suscetibilidade de cultivares de arroz irrigado (Oryza sativa) à deriva simulada do herbicida imazethapyr + imazapic

Objetivou-se neste trabalho avaliar a suscetibilidade dos cultivares de arroz irrigado IRGA 417, BR-IRGA 410, BRS Pelota e Qualimax 1 à simulação de deriva do herbicida imazethapyr + imazapic em função da época de início da irrigação por inundação. Para isso, foi conduzido experimento em casa de vegetação do Departamento de Fitossanidade da FAEM/UFPel, na estação de crescimento 2003/04. O delineamento experimental utilizado foi o de blocos casualizados, com quatro repetições. A deriva foi simulada pela aplicação de doses crescentes do herbicida imazethapyr + imazapic, correspondendo a 0; 3,125; 6,25; 12,5; 25; 50; e 100% da dose comercial (100 g ha-1), acrescido de Dash a 0,5% v/v, pulverizados sobre as plantas de arroz em estádios fenológicos V3 - V4, com inundação três dias antes ou após a aplicação dos tratamentos. A aplicação foi realizada com pulverizador costal, pressurizado a CO2, munido de barra com bicos 110.015 do tipo leque a uma pressão constante de 210 kPa, que proporcionou a aplicação de 150 L ha-1 de calda herbicida. A fitotoxicidade ocasionada pelo herbicida foi avaliada de forma visual aos 10, 15 e 20 dias após a aplicação dos tratamentos (DAT), utilizando-se escala percentual. Aos 25 DAT foi determinada a massa seca aérea e radical. O herbicida imazethapyr + imazapic causou fitotoxicidade aos cultivares de arroz IRGA 417, BR-IRGA 410, BRS Pelota e Qualimax 1, em deriva ocorrida em solo seco ou inundado. A massa seca da parte aérea e radical foi reduzida com o aumento da dose aplicada, independentemente da condição de aplicação. O cultivar IRGA 417 foi mais sensível à simulação de deriva de imazethapyr + imazapic.

Efeito de deriva simulada de herbicida inibidor de ALS nos componentes da produtividade do arroz irrigado

O objetivo deste trabalho foi quantificar os efeitos causados pela deriva simulada do herbicida imazethapyr + imazapic nos componentes da produtividade de grãos de arroz irrigado, em função da época de início da irrigação por inundação. Para isso, foi conduzido experimento no ano agrícola 2003/04, em condições de campo, no Centro Agropecuário da Palma (CAP/UFPel). O delineamento experimental utilizado foi o de blocos casualizados, com quatro repetições. A deriva foi simulada pela aplicação de doses crescentes do herbicida imazethapyr + imazapic, correspondendo a 0; 3,125; 6,25; 12,5; 25; 50; e 100% da dose comercial, acrescido de Dash a 0,5% v/v, sobre as plantas de arroz, cultivar BRS Pelota, em estádios fenológicos V3 - V4, com inundação três dias antes ou após a aplicação dos tratamentos. A aplicação foi feita com pulverizador costal, pressurizado a CO2, munido de barra com bicos 110.015 do tipo leque a uma pressão constante de 210 kPa, que proporcionou a aplicação de 150 L ha-1 de calda herbicida. As variáveis avaliadas foram: número de colmos, em estádio R3, número de grãos por panícula, número de espiguetas estéreis, peso de mil grãos e produtividade de grãos, determinados por ocasião da colheita. O herbicida imazethapyr + imazapic reduziu o número de colmos de arroz, o número de grãos e de espiguetas estéreis por panícula, o peso de mil grãos e a produtividade de grãos com o aumento da dose aplicada, quando esta ocorreu em cultura mantida no seco. A antecipação do início da irrigação por inundação, em lavouras semeadas com cultivar suscetível, evita possíveis prejuízos ocasionados por deriva de imazethapyr + imazapic no número de colmos, número de grãos e espiguetas estéreis por panícula, peso de mil grãos e produtividade de grãos.

Irrigation and Inoculation Treatments that Increase the Severity of Soybean Sudden Death Syndrome in the Field

The occurrence of sudden death syndrome (SDS), caused by the fungus Fusarium solani (Mart.) Sacc. f. sp. glycines (FSG) (syn. Fusarium virguliforme Akoi, O'Donnell, Homma and Lattanzi), is unpredictable in soybean [Glycine max (L.) Merr.] field trials making it difficult to evaluate soybean for resistance to the pathogen. Our objective was to evaluate the effect of field inoculation, soil compaction, and irrigation on the occurrence and severity of SDS symptoms. Six inoculation treatments were tested which included applications of FSG‐infested grain planted in the furrow with the soybean seed, broadcasted and incorporated into the soil before planting, or placed below the soybean seed just before planting. Soil was compacted by driving a tractor across the field once in early spring. Irrigation treatments were applied at combinations of growth stages V3, V7, R3, R4, and/or R5. Significant increases in foliar SDS severity were observed from inoculation and irrigation treatments (P < 0.05), but not from compaction treatments. The inoculation treatments that placed inoculum close to the seed resulted in the greatest foliar severity. Irrigation treatments during mid to late reproductive growth stages resulted in significant increases in SDS foliar symptom development. These results increase our understanding of what environmental conditions increase SDS field symptoms and will be useful to researchers establishing SDS field nurseries.