White Leghorn pullets of the Y1, Y2, and North Central Randombred (NCR) experimental stocks were used. Half of the birds of each stock had half of the upper and less of the lower mandible removed at 4 wk of age by making a V-shaped cut as viewed from the side (BT), whereas the other half retained their beaks intact (IN). Pullets were moved from growing pens to a layer house at 18 wk of age. Seventy-two cages and 30 floor pens were filled with 6 and 20 birds per unit, respectively. Pullets within a cage or floor pen were all of the same genetic stock and beak treatment. All measurements were performed within 10 wk after housing, except for mortality in cages.When kept in cages, genetic stocks did not show behavioral differences. However, in floor pens, stock differences were detected in feeding, crouching, and nonaggressive pecking. When kept in cages, the stocks also differed in 24-wk body weight, weight gain from 18 to 24 wk, and certain measures of fearfulness in both cages and pens, and in feather condition at 21 and 22 wk. Mortality from cannibalism and hen-days survival of caged IN pullets differed among genetic stocks. Mortality from cannibalism was absent among pullets kept in experimental floor pens.In cages, BT pullets were less active, gained less weight, and had lower incidence of cannibalism than IN pullets. Also, BT pullets were less nervous and had better feather condition than IN pullets. However, trimming beaks to prevent cannibalism was less effective in NCR pullets than in Y1 or Y2 pullets.In floor pens, BT pullets showed less activity and reduced feeding frequency and less fearfulness as indicated by duration of induced tonic immobility. Agonistic behaviors were not different between BT and IN pullets.From these results, beak trimming could be interpreted as having either stressful or stress-alleviating effects, depending on the criteria used. However, proper beak trimming appears beneficial when cannibalistic pecking is likely to be a problem. The relative benefits of beak trimming vary among genetic stocks.