UV-Vis Spectrophotometry Research Articles

Enhancement of Caffeine Concentration in Todolo Coffee Callus Cultures with L-Methionine and UV-Vis Spectrophotometry

Enhancement of Caffeine Concentration in Todolo Coffee Callus Cultures with L-Methionine and UV-Vis Spectrophotometry

Synthesis and evaluation of drug-loaded silver nanoparticles as hemostatic agents to halt uncontrolled bleeding

Objective The aim of this research study was to formulate a cost-effective, stable, less toxic and more efficacious intravenous nanoformulation that could rapidly augment the process of hemostasis. Significance Silver nanoparticles (AgNPs) evoked platelet activation, whereas alum (AM) neutralized the plasma proteins, causing blood coagulation. Tranexamic acid (TA) inhibited fibrinolysis and stabilized the formed blood clot. Methods The nanoformulation (NF) was subjected to characterization techniques such as UV-Vis spectrophotometry, FTIR, XRD, TGA and DSC analysis, which elucidated successful drug conjugation. Results Zeta-sizing confirmed the particle size of NF to be 256.6 nm with 0.497 PDI and a zeta potential of + 9.24 mV. In-vitro release profile exhibited first-order kinetics, indicating sustained release, conferring sustained release of NF for 12 h. NF was hemocompatible at the tested doses, as its extent of hemolysis was < 0.8% and < 1%, following EU and FDA guidelines, respectively. Ex-vivo studies revealed that NF recorded the highest viscosity, i.e., 36.5 cP, and maximum mass of clotted blood, i.e., 17.4 mg, in comparison to other combinations. In-vivo studies indicated a 100-fold dose reduction, i.e. 0.1 mg/kg, compared to the marketed formulation, Transamin® i.e. 10 mg/kg. 10 folds dose reduction i.e. 1 mg/kg, exhibited more efficacious results than Transamin®, owing to the synergistic effect and nano-sizing of components. Conclusion A safe, cost-effective, and relatively less toxic hemostatic nanoparticles were formulated, that can be intravenously administered to halt bleeding within seconds.

Extraction of Bioactive Compounds from Coffee Husk with Acetone Using Microwave Assisted Extraction Method and Analysis of Phenolic Compounds

Nowadays, the use of coffee husk is limited to animal feed and fertilizer. Therefore, a study was conducted using Robusta coffee husk waste as a raw material for research. The purpose of this study was to determine the effect of several variables and to determine the optimum conditions in the process of extracting bioactive compounds from coffee husk waste. Coffee husk waste will be extracted using the Microwave-Assisted Extraction (MAE) method with acetone solvent, with several variables, namely the ratio of material to solvent, microwave power, and extraction time. Analysis of the identification of phenolic compound content was then also carried out using UV-Vis spectrophotometry. The variables of material ratio, extraction time, and microwave power were proven to be interrelated so that they could produce total phenol at optimum conditions. The optimum conditions for extracting bioactive compounds from coffee husk waste were obtained at a material ratio of 0.04 g/ml; time of 9 minutes; and power of 300 watts, with a total phenol yield of 8.65 GAE/g sample.

Cyto and genotoxicity induced by acute exposure to Novacron® Bold Deep Navy dye on Astyanax lacustris can be reduced after treatment through a vertical flow constructed wetland system.

Reactive dyes used in the textile industry can be harmful to aquatic organisms. This study aimed to evaluate the potential cyto/genotoxic effects of the Novacron® Bold Deep Navy reactive dye on fish Astyanax lacustris before and after treatment through a vertical flow constructed wetland system (VFCW). Micronucleus test (MN), cellular morphological changes (CMC), and the comet assay were used to assess the effects of the dye in erythrocytes. During the experiment, A. lacustris specimens were exposed to sublethal concentrations (10, 20, 30, and 40mg/L) of Novacron® Bold Deep Navy dye for 96h. Results showed that the dye caused MN formation (27), DNA damage, and several types of CMC (3513) in all tested concentrations on A. lacustris. Notably, analyses were significant (p < 0.05) for all concentrations, except in the frequency mean of DNA damage at 10mg/L. The bioremediation of the dye carried out by the VFCW system proved to be efficient, as confirmed by UV-Vis spectrophotometry, liquid chromatography-mass spectrometry (LC-MS), and physicochemical analysis. Thus, the Novacron® Bold Deep Navy dye poses a potential risk of genotoxicity and cytotoxicity for aquatic organisms, and the VFCW system proved to be efficient in treating the dye.

Exploring ultraviolet and nitrous acid-induced mutagenesis of Rhizopus oligosporus for extracellular inulinase production under solid-state culture.

This study details the synthesis and optimization of extracellular inulinase through solid-state fermentation using improved strain of Rhizopus oligosporus. The wild-type was procured from IIB culture bank and subsequently enhanced through UV-radiation and Nitrous acid treatments. The resulting mutant strain was subjected to further optimization for heightened enzyme production via solid-state fermentation. The substrate, pressmud (20g), and moisture content (20 mL molasses) were carefully selected. Key parameters such as inoculum size, incubation time, and inulin concentration were optimized to maximize enzyme yield. Mutation procedures involved optimizing spore suspension, UV-radiation exposure duration, and the distance of culture plate from UV rays. Nitrous acid treatment, followed by resistance development using L-cysteine HCl, further refined the mutant strains. Evaluation through UV-Vis spectrophotometry and comprehensive characterization using SEM, FTIR, and XRD were conducted to compare the results between the wild-type and mutant strains. The mutant strain exhibited significantly higher inulinase activity (111 ± 0.49 U/ml) as compared to its wild-type counterpart (59 ± 0.65 U/ml), indicating successful enhancement through the applied mutation techniques. The molecular weight of the inulinase enzyme produced from mutant strain and wild-type was 83 and 86kDa respectively. These findings suggest potential industrial applications of the improved strain in various biotechnological processes.

Introduction of curvature index and improvement of calibration curves used in ion chromatography (IC), atomic absorption spectrometry (AAS), UV-Vis spectrophotometry and catalytic combustion

Introduction of curvature index and improvement of calibration curves used in ion chromatography (IC), atomic absorption spectrometry (AAS), UV-Vis spectrophotometry and catalytic combustion

Potassium, sodium, and proximate analysis of steamed sponge cake from durian seed flour (Durio Zibethinus)

Steamed sponge is a conventional cake that enjoys widespread popularity and is highly cherished by the general population. The cake is commonly made using wheat flour. Given the present increase in demand for wheat flour, an alternative option for making steamed cakes is to utilize durian seed flour instead of additional wheat flour. Durian seed flour, which has excellent nutritional value for health, is made from processed durian seeds. This study aims to find out how much potassium and sodium are present in steamed sponge cake based on durian seed flour and to ascertain the quantities of fat, protein, and carbohydrate. Atomic absorption spectrophotometry was used to determine the levels of potassium and sodium in steamed sponge cake. The sodium levels were analyzed at a specific wavelength of 766.5 nm, whilst the potassium levels were analyzed at a distinct wavelength of 589 nm. The proximate analysis involves determining the protein content using the micro-Kjeldahl method, the fat content using the soxhletation method, and the carbohydrate content using the UV-VIS spectrophotometry method at a wavelength of 460 nm. The study found that steamed sponge cake contains approximately 44% carbohydrates, 28% protein, and 23% fat in terms of its nutritional composition. Furthermore, the potassium concentration measured 284 mg per 100 grams, whereas the sodium value was 62 mg per 100 grams.

Herb-Fortified Arabica Cascara Infusion as a Functional Beverage

Cascara is a product derived from the drying of Arabica coffee pulp. It has potential as a functional beverage with health benefits. This study aims to produce a functional beverage from cascara and analyze its organoleptic properties, phytochemical content, antioxidant activity, total polyphenols, and caffeine content of cascara infusion fortified with medicinal plants. The methods utilized in this study included drying, grinding, and sieving, as well as the formulation of cascara with lemongrass (Cymbopogon citratus) and kaffir lime peel (Citrus hystrix) fortificants. The formulations included V1 (C : S = 75% : 25%); V2 (C : J = 75% : 25%); V3 (C : S : J = 50% : 25% : 25%); and V4 (C : S : J = 75% : 12,5% : 12,5%). The analysis included organoleptic evaluation, cascara brewing temperature and time optimization, gravimetric method moisture content evaluation, pH measurement, phytochemical content evaluation, DPPH method antioxidant activity assay, Folin-Ciocalteu method total polyphenol content measurement, and UV-Vis spectrophotometry caffeine content evaluation. The hedonic test results indicated that the most preferred sample was V1, with a score of 3.90 out of 5.00. Moisture content analysis showed that cascara contains 13.36 ± 0.0007% moisture, lemongrass stalks 11.10 ± 0.0043%, and kaffir lime peel 7.63 ± 0.0004%. The pH of all variants had met the standard with a range of 4.18 ± 0.05 - 4.24 ± 0.08. The phytochemical analysis indicated that the control variable of cascara infusion (C) and V2 contained alkaloids, phenolics, and tannins. Meanwhile, V1 contained alkaloids, phenolics, tannins, and terpenoids. All cascara infusion variants contains antioxidant activity ranged between 78,25 ± 0,25 - 79,50 ± 0,63%; total polyphenol 494,25 – 1.500 mg GAE/100g; and caffeine content 392,53 – 409,53 mg/100g. In conclusion, the study found that cascara infusion V1 was the most preferred by taste testers, and all the infusions were rich in antioxidants and other beneficial compounds.

Production Of Ready-To-Drink Cascara Kombucha Fortified With Coconut Water (Cocos Nucifera L.) and Red Dragon Fruit (Hylocereus Polyrhizus)

Cascara is coffee pulp which contains caffeine, chlorogenic acid and phenolic compounds, but has not been utilised optimally. Cascara can be processed into ready-to-drink cascara kombucha products, which are beneficial for the body. This research aims to determine the effect of adding coconut water (Cocos nucifera L.) and red dragon fruit (Hylocereus polyrhizus) on the alcohol content, total phenolics, caffeine, minerals, antioxidant activity and sensory hedonic content of ready-to-drink cascara kombucha. This research method was carried out in 3 stages including optimizing fermentation time and SCOBY starter concentration based on pH value; fortification ratio optimization; and product analysis includes alcohol content by dichromate method, total phenolics by Folin-Ciocalteu method, caffeine by UV-VIS spectrophotometry method, minerals by AAS spectrophotometry method, antioxidant activity by DPPH method, and sensory hedonic. The research results showed that the best time was 12 days, pH value 3.42 at 10% SCOBY starter concentration. The ratio of cascara kombucha : fortificant is 1:1 resulting in an alcohol content of 0.23-0.43%. The highest levels for total phenolics, caffeine, and minerals were 656.32 mg/kg; 35.53 ppm; and 732.83 mg/100g respectively. The highest antioxidant activity was obtained from the red dragon fruit fortified cascara kombucha product with a yield of 89.69%. The highest color and aroma attributes were obtained from the cascara kombucha product fortified with a mixture of coconut water and red dragon fruit, while the highest value for the taste attribute was obtained from the cascara kombucha product fortified with red dragon fruit.

Green Synthesis of Terminalia ferdinandiana Exell-Mediated Silver Nanoparticles and Evaluation of Antibacterial Performance

This study uses a novel method in which extracts from different parts of a single plant are used to synthesize well-defined silver nanoparticles (AgNPs) to address the lack of capping agents in certain plant extracts. We focused on synthesizing AgNPs with enhanced biomedical activity using aqueous leaves and fruit extracts of Terminalia ferdinandiana Exell, a plant native to northern Australia that is known for its high phenolic content and associated health benefits. The impact of using parameters such as the Ag+ ion-to-extract ratio and pH on AgNP synthesis was examined. The formation of AgNPs was confirmed using UV–visible spectrophotometry, transmission electron microscopy, and dynamic light scattering. The AgNPs synthesized at a pH of 8 and 1:25 Ag+/extract ratio exhibited the lowest particle size and polydispersity index. The AgNPs synthesized with leaf extract (AgKL) were monodisperse and exhibited a smaller hydrodynamic diameter (37 nm) compared to the fruit extract nanoparticles (AgKP), which were polydisperse and larger (147 nm). Phytochemicals in T. ferdinandiana aqueous leaf extract act as effective capping and stabilizing agents, enabling the synthesis of small-sized and homogenous AgNPs, which the fruit extract alone could not achieve. The in vitro bioactivity was evaluated using antioxidant and antibacterial assays and compared with the crude extract. Both the AgNPs and T. ferdinandiana extracts demonstrated strong 2,2 diphenyl-1-picrylhydrazyl radical scavenging activity. However, only AgKL showed excellent antibacterial activity against Gram-negative and Gram-positive bacteria based on minimum inhibitory and bactericidal results. Mixing 50% leaf extract with fruit extract resulted in well-stabilized NPs (AgKPL) with a hydrodynamic diameter of 33.4 nm and superior antibacterial properties. These results indicate that AgKL and AgKPL have significant potential for pharmaceutical and biomedical applications.

Testing of UV Protection Activity, SPF Determination, and Irritation Assessment of Coastal Vegetation Ipomoea Pes-Caprae

Total solar radiation contains approximately 10% ultraviolet light. Ultraviolet rays have benefits such as providing warmth, light, and helping to synthesise vitamin D3. However, ultraviolet rays can also have negative impacts on the skin such as browning, hyperpigmentation, erythema, and premature ageing. Natural sunscreens with high SPF are useful in reducing the negative effects of ultraviolet rays. This study aims to determine anti-UV activity, SPF value, and assessment of human skin irritation or allergy to sunscreen cream from Ipomoea pes-caprae leaves. Anti-UV activity test and SPF value determination were carried out in vitro using UV-Vis Spectrophotometry. Irritation testing was conducted using the patch test method for 6 hours. The results showed that Ipomoea pes-caprae has anti-UV-A and anti-UV-B activities. Ipomoea pes-caprae leaf extract provides extra protection at a concentration of 2000 ppm. The irritation test of the cream on human skin found no skin abnormalities such as edema, urticaria, irritation and edema.

Physiochemical, Radiative Transitions and Thermoluminescence Characterizations of Nano Ce-doped Monoclinic Barium Titanate

AbstractThis article reports the preparation, analytical, and thermoluminescence (TL) characterization of BaTi5O11 doped with different amounts of Ce. The Sol–gel technique was used to synthesize the doped nanocrystals. The prepared samples were examined by XRD, EDX, UV–Vis spectrophotometry, SEM, and TEM techniques to determine their analytical features. The samples with 0.7% Ce doping showed the best thermoluminescent behavior among the samples we made. The glow curves analysis showed that there were seven peaks that overlapped with each other. The thermal activation energy values of the carrier traps are 0.93, 1.08, 1.16, 1.23, 1.29, 1.35, and 1.38 eV. This study displayed that 0.7% Ce-doped BaTi5O11 can be used in monitoring high doses radiation applications.

Unleashing of cytotoxic effects of thymoquinone-bovine serum albumin nanoparticles on A549 lung cancer cells.

This research examines the cytotoxic consequences of thymoquinone-loaded bovine serum albumin nanoparticles (TQ-BSA NPs) on the A549 lung cancer cell line. UV-visible (UV-Vis) spectroscopy, Fourier transform infrared spectrophotometer (FT-IR), scanning electron microscopy (SEM), and dynamic light scattering (DLS) were employed to verify the biogenic TQ-BSA NPs' size, shape, and distribution. UV-Vis spectrophotometry indicated peaks at 200-300 nm, 500-600 nm, and a prominent peak at 700-800 nm, confirming the presence of TQ-BSA NPs. The polydispersity index, as confirmed by DLS, indicated a solvent distribution in water, accompanied by a zeta potential value of 126.2 ± 46.8 mV. The average size of TQ-BSA NPs was confirmed to be 187 ± 8 nm by SEM. TQ-BSA NPs reduce colony formation in the A549 lung cancer cell line in a dose-dependent manner relative to the control group. Protein expression analysis indicated that TQ-BSA NPs promoted programmed cell death by increasing pro-apoptotic levels and decreasing anti-apoptotic levels. TQ-BSA NPs demonstrated inhibition of cancer cell proliferation and promotion of apoptosis and exhibited significant efficacy against cancer cells at low concentrations. As a result, they have the makings of a promising chemotherapeutic agent for low-dose, long-term administration.

Spectroscopy-based analysis of the effect of Maillard reaction products on oxidative stability of carp myoglobin.

As a pro-oxidant, myoglobin (Mb) can induce lipid oxidation in meat. In this study, Fourier-transform infrared spectroscopy (FTIR), circular dichroism (CD), fluorescence spectroscopy, UV-vis spectrophotometry, and fluorescence microscopy were used to evaluate the impact of Maillard reaction products (MRPs) formed from glucose and lysine on the oxidative stability of carp Mb. MRPs were found to inhibit the auto-oxidation of Mb, reducing MetMb production by 8.45%. The static quenching of fluorescence indicated that MRPs interacted with Mb through hydrogen bonding and van der Waals forces, thereby enhancing the α-helical content by 11.57% and reducing the random coil content by 2.72%. The enhanced stability of this advanced structure helps to minimize the exposure of amino acid in the side chain and prevent the formation of MetMb. Fluorescence microscopy showed that MRPs reduce porphyrin ring degradation, consequently decreasing heme iron release. It is evident that MRPs play a crucial role in maintaining Mb structure stability and inhibiting its oxidation.

Influence of soil moisture availability on dissolved organic matter properties and microbial carbon content in Poa pratensis L. rhizosphere

The goal of the work was to assess changes in microbial carbon content and the properties of dissolved organic matter (DOM) of the Poa pratensis L. rhizopshere in response to the effects of soil drying and waterlogging. The vegetation experiment without plants and with dense sowing in vegetative vessels on Haplic Chernozem surfacewas used in the work. The properties of dissolved organic matter were evaluated based on the study of water-extractable organic matter (WEOM), in which the optical properties were assessed by UV-Vis spectrophotometry and spectrofluorimetry. Soil microbial carbon content was assessed by fumigation method. It has been shown that microbial carbon content in the bulk Chernozem does not depend on soil moisture, while in the rhizosphere it increases with drying and decreases with optimal and excess moisture. Soil moisture greatly affects WEOM properties of the bulk Chernozem, whereas the rhizosphere properties are influenced by the activity of the plant. Despite the lower microbial biomass compared to the bulk soil, the microbiome of P. pratensis L. rhizosphere is capable of more intense transformation of soil organic matter. The dynamic properties of P. pratensis L. rhizosphere ensure its more active and sustainable functioning compared to bulk soil.

Evaluation of hydrogen peroxide diffusion in the pulp chamber, bleaching efficacy, and physicochemical properties of an experimental in-office bleaching gel containing a hydroxyapatite-capsaicin composite.

This study assessed the hydrogen peroxide (HP) diffusion into the pulp chamber, bleaching efficacy (BE), surface roughness (Ra), and Knoop microhardness (KHN) of an experimental bleaching gel containing a hydroxyapatite-capsaicin composite (HAp-CAP). Human premolars were randomly assigned to three groups (n = 9) based on the dental bleaching gel used (50min; one session): only 35% HP, 35% HP + HAp-CAP, and not exposed to bleaching (negative control; NC). HP diffusion (µg/mL) into the pulp chamber was assessed using UV-Vis spectrophotometry, and BE (ΔEab, ΔE00, and WID) was evaluated using a digital spectrophotometer. Human molars were used to determine Ra and KHN before and 7days after the bleaching procedure, and for Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) analyses. A paired t-test or t-test was used for statistical analysis (α = 0.05). A higher amount of HP into the pulp chamber and significant BE (ΔEab, ΔE00, and WID) were observed for 35% HP and 35% HP + HAp-CAP compared to NC (p < 0.001), with no significant difference between them (p > 0.05). SEM and EDS demonstrated material deposition on the enamel surface after bleaching for 35% HP + HAp-CAP, which helped maintain the KHN (p > 0.05). Furthermore, Ra increased for both groups (p < 0.05). The 35% HP + HAp-CAP did not decrease HP diffusion into the pulp chamber, reduced KHN values, and increased Ra. Material deposition on the enamel surface was observed, and BE was effective.

Synthesis of derived Cu(II) complex from Schiff base of Vitamin B6 and antihypertensive hydralazine: DNA interaction, biological application and molecular docking studies

A novel Schiff base ligand (E)-5-(hydroxymethyl)-2-methyl-4-((2-(phthalazin-1-yl)hydrazineylidene)methyl)pyridin-3-ol was synthesized through the condensation of antihypertensive hydralazine and Pyridoxal (Vitamin B6) ligands. This fabricated Schiff base (L) was a precursor for developing a unique Cu(II) complex. TheSchiff base ligand and Cu(II) complex structureswere characterized using various analytical tools, including IR, UV–Vis Spectroscopy and NMR. An octahedron geometry of this complex was proposed for the assigned molecular formula [Cu(L)2].3H2O. The molecular structures of theSchiff base ligand and its Cu(II) complex have been optimized using the B3LYP functional and the 6-311G++(d,p) basis set. Investigating the interaction of this prepared Cu(II) complex with calf thymus DNA (CT-DNA) molecules was operated using UV–Vis spectrophotometry, viscosity measurement, electrochemical studies, gel electrophoresis, and molecular docking. In vitro cytotoxicity was conducted using the MTT assay on four human tumor cell lines including hepatocellular carcinoma, estrogen-dependent breast cancer, melanoma, triple-negative breast cancer as well as healthy human skin fibroblasts.

Optical and Methanol Sensing Properties of Al-doped ZnO Thin Film

The study investigates the optical and electrical properties of undoped and aluminum (Al)-doped zinc oxide (ZnO) films, focusing on their performance as gas sensors and their potential applications. Optical analysis, conducted using UV-visible spectrophotometry, reveals that 1% Al-doped ZnO films exhibit the highest transmittance of 91%, indicating superior optical clarity and suitability for applications like solar cell electrodes. In contrast, 3% Al-doped ZnO films show significantly lower transmittance due to increased light scattering and photon absorption. The bandgap of ZnO films decreases with higher Al doping concentrations, from 3.3 eV for undoped ZnO to 3.15 eV for 3% Al-doped ZnO, suggesting enhanced electrical conductivity due to reduced bandgap. The extinction coefficient data demonstrate that 2% Al-doped ZnO has the highest extinction coefficient, reflecting improved light absorption and scattering properties. Electrical characterization through I-V curves indicates that 1% Al-doped ZnO films have higher current (121 µA) compared to undoped (431 µA) and higher doping concentrations, attributed to enhanced carrier concentration and mobility. Sensitivity tests show that 2.5% Al-doped ZnO films exhibit the highest sensitivity to methanol vapor, with a significant reduction in resistance compared to 0.5% Al-doped ZnO films. Resistance measurements with varying methanol volumes reveal a rapid decrease upon gas introduction, stabilizing and then increasing as the gas is removed. Sensitivity analysis indicates that 100 µL methanol provides the highest sensitivity (97%) at 60°C, while 2% Al-doped ZnO films show consistent sensitivity at 60 °C and 100 °C, but not at 80 °C.

Effect of discharge pulse frequency on the microstructure and field-emission performance of CNX films deposited by pulse cathode arc evaporation

CNX thin films were prepared by a pulse cathode arc technique using nitrogen as a dopant at different frequencies. Microstructure, compositions, and morphology of CNX thin films were investigated in the dependence of discharge pulse frequency by Raman spectroscopy, x-ray photoelectric spectroscopy (XPS), atomic force microscopy, scanning electron microscopy, UV–vis spectrophotometry, Hall measurements, and field-emission test. The results of Raman and XPS showed that the Csp2 cluster size of CNX films decreases and then increases and decreases with discharge pulse frequency increasing. The Csp2 cluster size of the films prepared at 9 Hz was the largest. The size of Csp3 content increases for the CNX films at the high discharge pulse frequency. The variation of C–N bonding content with frequency in CNX films is similar to that of Csp2. CNX thin films prepared at a frequency of 9 Hz were improved field-emission properties corresponding to the variation of microstructure parameters (the size and ordering of Csp2 clusters) and compositions (C–N bonds). The excellent field-emission properties, such as a low turn-on electric field of 2.1 V/μm and a high field-emission current density of 517.34 μA/cm2, were obtained.

Exploring the efficacy of Pongamia pinnata-induced silver nanoflowers for efficient adsorptive degradation of malachite green dye

In this study, silver nanoflowers were synthesized due to catalytic reduction of silver ions by phytochemicals of Pongamia pinnata seed cake extract (PSCAgNPs) in 24 h and subsequently used for removal of malachite green (MLG) dye. PSCAgNPs were characterized by UV–Visible spectrophotometry, scanning electron microscopy (SEM), energy-dispersive X ray spectroscopy (EDAX), Fourier Transformed Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD) and BET analysis. The synthesized PSCAgNPs were floral in appearance, of size 18.82—23.70 nm with SPR at 430 nm and crystalline in nature. PSCAgNPs adsorbed 90.01 ± 0.17% of 50 mg/L MLG post one factor at a time analysis. Adsorption followed pseudo-second-order kinetics (k1 = 2.5E-03 g/mg.h), was observed as unilayer chemisorption as the equilibrium data best fitted Langmuir isotherm (Langmuir coefficient = 0.1724 L/mg, R2 = 0.9945). Upon interaction of MLG by PSCAgNPs, hydroxylation of MLG leads to formation of MLG carbinol in the first stage of adsorptive degradation, as evident from LCMS chromatograms.Graphical