UV Spectroscopic Methods Research Articles

Development and validation of UV spectroscopic method for estimation of sacubitril and valsartan combination (LCZ696) in bulk and pharmaceutical tablet dosage forms

Sacubitril and valsartan combination is a member of a new class of agents called angiotensin receptor-neprilysin inhibitors (ARNI’s) which combine neprilysin inhibitor and angiotensin receptor blocker (ARB). It is currently indicated for treating patients with heart failure with reduced ejection fraction (HFrEF) in place of an angiotensin-converting enzyme (ACE) inhibitor or ARB alone. The aim of present investigation is to develop a simple UV spectrophotometric method for the determination of sacubitril and valsartan combination in its pure form and pharmaceutical tablet formulations in 0.1N HCl (pH 1.2) and pH 6.8 phosphate buffer, and further to validate the developed method. The combination in bulk was estimated at λmax of 253 nm in 0.1N HCl and pH 6.8 phosphate buffer. The observed λmax was close to the calculated λmax predicted using Woodward Fieser rules. The method was validated using analytical parameters like linearity, precision, and accuracy as per guidelines laid down by International Conference on Harmonization (ICH). Beer’s law was obeyed in the concentration range of 1–20 μg/mL in both media with correlation coefficient value of 0.999. The accuracy was found between 99-101% in both media. The method showed good reproducibility with % RSD values less than 2. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.5353 μg/mL, 1.6222 μg/mL in 0.1N HCl and 0.395 μg/mL, 1.198 μg/mL in pH 6.8 phosphate buffer respectively indicating its sensitivity. Molar absorptivity of the drug was found to be 3.84x104, 3.68x104 L mole-1 cm-1 and Sandell’s sensitivity values were found to be 0.0249 and 0.025 μg cm-1/0.001 absorbance unit in 0.1 N HCl and pH 6.8 phosphate buffer respectively. The assay values of the drugs in pharmaceutical dosage forms were also found close to the labelled claim. The results demonstrated that the procedure is accurate, precise, and reproducible besides being simple, economical, and less time consuming and hence, suitably applied for routine analysis of sacubitril and valsartan combination in bulk, marketed tablet dosage forms and in vitro dissolution samples.

Ultrahigh-Sensitivity Capillary Electrophoresis Analysis of Trace Amounts of Nitrate and Nitrite in Environmental Water Samples

The role of nitrite (NO2−) and nitrate (NO3−) is essential in the global nitrogen cycle. Monitoring their concentration in environmental and industrial aqueous samples, surface water, soil, food and agricultural products are of high importance. Especially, the effect of anthropogenic emission, i.e., intensified agriculture is essential due to the overuse of nitrogen, phosphorus and potassium fertilizers. The most widely utilized methods for nitrate and nitrite determination are colorimetry, potentiometry, UV absorption and liquid chromatography. Among them, UV spectroscopy is the most frequently used technique due to the fact of its versatility and simplicity. However, there are industrial and academic needs to develop new methods to overcome some drawbacks of the currently used techniques such as an inadequate limit of detection and potential interferences with organic compounds in the sample. In this paper, we report on the development of a new analytical method based on capillary electrophoresis separation with high-sensitivity UV detection, capable of measuring trace concentrations of nitrite and nitrate well below the current limits of UV spectroscopy methods. During the development process special attention was paid to practical aspects, i.e., the method was tested to quantify nitrate and nitrite in various surface water samples.

Development of simple, precise UV spectroscopic methods for the estimation of Lamotrigine in bulk and marketed tablet

Development of simple, precise UV spectroscopic methods for the estimation of Lamotrigine in bulk and marketed tablet

Development of simple, precise UV spectroscopic methods for the estimation of Lamotrigine in bulk and marketed tablet

Two Simple and precise UV spectroscopic methods were developed for the estimation of lamotrigine in bulk and marketed tablets. The methods developed by using two solvent systems viz., Acetonitrile: Distilled water (1:1) and Methanol: 0.1N HCl (3:1) were validated as per ICH guidelines. The two proposed solvent systems validated for linearity, accuracy, precision, robustness, ruggedness and solution stability. The percent recovery in the marketed tablet formulation was found to be good agreement with the label claim. The methods validated statistically and the results suggest these methods can employed for the routine analysis of lamotrigine in bulk and marketed tablet formulations.

Analytical Method Development and Validation for Simultaneous Estimation of Naproxen and Esomeprazole in Pharmaceutical Dosage Forms

Introduction: An accurate, specific and precise reverse phase UV spectrophotometric method was developed for the simultaneous estimation of Naproxen and Esomeprazole in tablet dosage form. Naproxen and Esomeprazole tablets were used to relieve the spondylitis, osteoarthritis and rheumatoid arthritis in adults’ juvenile idiopathic arthritis in children.
 Methods: Simple and accurate UV spectroscopic method was developed using Naproxen and Esomeprazole tablet and results were tabulated.
 Results and Discussion: The optimum conditions were established for the analysis of the drug. The maximum wavelength of Naproxen (λmax) was found to be 231nm and for Esomeprazole was 301nm respectively. The linearity of the method developed was in the range 10-50 μg/ml for Naproxen and 30-70 μg/ml for Esomeprazole. Calibration curves for Naproxen and Esomeprazole showed a linear relationship among the absorbance and concentration. The line equation observed for Naproxen is Y= 0.0374X + 0.0805 with r2 of 0.9936 and for Esomeprazole drug was 0.0377 X+0.0576 with R2 of 0.9962 . Validation parameters were performed as per ICH guidelines for all validation parameters like linearity, accuracy, precision, LOD and LOQ. The results of LOD and LOQ were found to be within the range.
 Conclusion: The proposed method for Naproxen and Esomeprazole was simple, yet sensitive and precise for repeated analysis of Naproxen and esomeprazole in bulk and tablet formulations.

Study into the hydrolysis of polyhexamethylene guanidine hydrochloride

The development of new preparations for managing skin lesions is a task requiring a complex research approach. Thus, one promising direction consists in the creation of new bases for wound-healing drugs for external application. Chemical compounds that can be used as such bases include polymeric hydrogels, representing spatially cross-linked macromolecules that swell in a solvent. This property provides an opportunity to load hydrogels with drugs of both synthetic and herbal origin. A search for a gel-forming polymer acting not only as a drug carrier, but also as a healing agent presents a relevant research task. In a previous work, the authors obtained polyhexamethylene guanidine hydrochloride hydrogels by crosslinking terminal amino groups with formaldehyde. The conducted studies of the wound-healing capacity of the obtained hydrogels and compositions on their basis confirmed their comparability with such widely-used agents, as levomecol, bepanthene, etc. In addition, the obtained compositions were found to exhibit their own activity. Therefore, hydrogels based on polyhexamethylene guanidine hydrochloride can be used as a promising platform for drug design. In this work, the destruction products released during hydrolysis of the hydrogel under study were investigated. IR and UV spectroscopy methods were applied to evaluate the concentration of hydrogel destruction products over time. A mechanism of hydrogel destruction yielding the initial polyhexamethylene guanidine hydrochloride and formaldehyde in a gem-diol form is proposed.

New anti-inflammatory and non-cytotoxic metabolites of methylstenbolone obtained by microbial transformation

A synthetic anabolic–androgenic steroid, methylstenbolone (1), was structurally transformed into a series of nine analogues, 2,17α-dimethyl-7α,17β-dihydroxy-5α-androst-1-en-3-one (2), 2,17α-dimethyl-15β,17β-dihydroxy-5α-androst-1-en-3-one (3), 2,17α-dimethyl-6α,9α,17β-trihydroxy-5α-androst-1-en-3-one (4), 2-methyl-17β-hydroxy-17α-(hydroxymethyl)-5α-androst-1-en-3-one (5), 2-methyl-11β,17β-dihydroxy-17α-(hydroxymethyl)-5α-androst-1-en-3-one (6), 2-methyl-17β-hydroxy-17α-(hydroxymethyl)-5α-androst-1-en-3,6-dione (7), 2-methyl-17β-hydroxy-17α-(hydroxymethyl)-5β-androst-1-en-3,6-dione (8), 2,17α-dimethyl-7β,17β-dihydroxy-5α-androst-1-en-3-one (9), and 2,17α-dimethyl-12β,17β-hydroxy-5α-androst-1-en-3,7-dione (10) by fungal cell suspension cultures, Macrophomina phaseolina and Cunninghamella blakesleeana for the first time. Among those, compounds 2–4 and 6–10 were identified as new. Herein, spectral data of metabolite 5 was reported for the first time. Their structures were elucidated by NMR, MS, UV, and IR spectroscopic methods. Substrate 1 (IC50 10.1 ± 0.3 µg/mL) was identified as a potent anti-inflammatory agent against nitric oxide (NO) production. Its transformed products 3 (IC50 as 27.8 ± 1.1 µg/mL) and 9 (26.9 ± 0.4 µg/mL) displayed good inhibition. Compounds 2 (IC50 = 45.9 ± 0.8 µg/mL) and 6 (IC50 = 36.6 ± 1.2 µg/mL) were also active moderately against NO production, in comparison to standard LNMMA (IC50 = 24.2 ± 0.8 µg/mL). Cytotoxicity assay showed 1 was active to cancer cell line MCF7 (IC50 = 12.26 ± 0.35 µg/mL), compared to the standard Doxorubicin having IC50 as 1.25 ± 0.11 µg/mL. However, it is also toxic to human normal cell line (BJ) with IC50 as 8.69 ± 0.02 µg/mL. More importantly, all transformed products are non-cytotoxic on BJ. Therefore, biotransformation can be an efficient approach to reduce the toxicity of methylstenbolone.

Novel UV Spectroscopic Methods for Simultaneous Assessment of Empagliflozin, Linagliptin and Metformin in Ternary Mixture

Novel UV Spectroscopic Methods for Simultaneous Assessment of Empagliflozin, Linagliptin and Metformin in Ternary Mixture

UV spectrophotometric analysis for method development and validation of Beclofen

Baclofen is a γ-aminobutyric acid (GABA) close derivative which is primarily used in the treatment of spastic movement disorder. Estimation and determination of drug in any biological fluid, bulk and pharmaceutical formulation is important for safety, quality and therapeutics purpose which can be estimated by various methods including UV-visible spectroscopy, HPLC, colorimetry, solvent melting method etc. The main objective of this study was to develop and validate new UV-visible spectroscopy method for estimation of baclofen in HCL and phosphate buffer as per ICH guidelines. The UV spectroscopy method for the estimation of Baclofen was successfully prepared and validated with percentage recovery in the range of 97.41 – 102.62 %. The developed method was found to be precise, accurate, rugged and robust after evaluating the validation parameters.

STUDY OF THE EFFECT OF ZCO CERAMIC PHASE COMPOSITION ON OPTICAL AND THERMOPHYSICAL PROPERTIES

The work is devoted to the study of optical and thermophysical properties of promising materials for nuclear power of a new generation - inert matrices of nuclear dispersed fuel based on ZrO2 – CeO2 (ZCO) doped with Y2O3. Interest in this class of materials is due to great prospects in the field of replacing the traditional type of nuclear fuel with uranium-free dispersed nuclear fuel, which is based on a technological solution using plutonium as a fissile element, placed in a dispersed inert matrix based on oxide or nitride ceramics. The purpose of this work is to study the effect of doping of ZCO ceramics with Y2O3 on the optical and heat-conducting properties. During the study, it was found that an increase in the concentration of the Y2O3 dopant leads to the formation of a highly ordered CeZrO4 phase, the presence of which leads to the appearance of an additional absorption band in the region of 550 – 650 nm, as well as a decrease in the transmission value in the region of 400 – 700 nm. To evaluate the results obtained, the methods of optical UV spectroscopy and heat flux determination were applied. It has been found that doping with Y2O3 leads to the formation of a highly ordered CeZrO4 phase, the formation of which leads to the appearance of additional spectral absorption lines, as well as a shift in the band gap to the region of low energies. During analysis of the thermophysical parameters, it was found that a change in the phase composition leads to an increase in the thermal conductivity coefficient by more than 40 – 60 %.

DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR ESTIMATION OF ABACAVIR IN TABLET DOSAGE FORM

Objective: To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Abacavir in tablet dosage form.
 Methods: The drug is freely soluble in analytical grade methanol. The drug was identified in terms of solubility studies and on the basis of melting point done on the melting point apparatus of Equiptronics. It showed absorption maxima were determined in analytical grade methanol. The drug obeyed the Beer’s law and showed good correlation of concentration with absorption, which reflect in linearity. The UV spectroscopic method was developed for estimation of Abacavir in tablet dosage form and also validated as per ICH guidelines.
 Results: The drug is freely soluble in analytical grade methanol, slightly soluble in water and practically insoluble in ethanol. So, the analytical grade methanol is used as a diluent in method. The melting point of Abacavir was found to be 164-165 ˚C (uncorrected). It showed absorption maxima 256 nm in analytical grade methanol. On the basis of absorption spectrum the working concentration was set on 15µg/ml (PPM). The linearity was observed between 5-25 μg/ml (PPM). The results of analysis were validated by recovery studies. The recovery was found to be 98.75, 101 and 99.17% for three levels respectively. The % RSD for precision was found to be 0.32% and for Ruggedness is 0.46%
 Conclusion: A simple, rapid, linear, accurate, precise and economical UV Spectroscopic method has been developed for estimation of Abacavir in tablet dosage form. The method could be considered for the determination of Abacavir in quality control laboratories.

Development and Validation of a New Simple and Rapid UV Spectroscopic Method for Cefalexin and Potassium-Clavulanate in Pure and Dosage Form

Development and Validation of a New Simple and Rapid UV Spectroscopic Method for Cefalexin and Potassium-Clavulanate in Pure and Dosage Form

Current aspects of Quality Control and Standardization of <I>Eleutherococcus senticosus</I> Medicines

Introduction. Nowadays, herbal medicines are becoming more and more popular. In this regard, herbal medicinal preparations based on rhizomes and roots of Eleutherococcus senticosus (Rupr. & Maxim.) Maxim., syn. Acanthopanax senticosus (Rupr. & Maxim.) Harms, are of particular interest. Its have an adaptogenic, immunomodulatory effects mainly due to the presence of phenyl propane compounds: eleutherosides B, D, E, E1. The current pharmacopeial monographs FS.2.5.0053.15 "Eleutherococcus senticosus rhizomes and roots" and FS.3.4.0009.18 "Eleutherococcus senticosus rhizomes and roots liquid extract" in The State Pharmacopoeia of the Russian Federation XIV Edition, in our opinion, does not allow to fully objectively and uniformly assess the quality of raw materials and preparations.Aim. The aim is substantiation of new approaches to quality control of raw materials and preparations of Eleutherococcus senticosus, consisting in changing the conditions of sample preparation and analysis of active biological compounds in comparison with pharmacopoeial methods.Materials and methods. Samples of the medicinal preparation "Eleutherococcus extract liquid" of a domestic manufacturer (5 lots) and experimental preparation "Eleutherococcus syrup". When performing high performance liquid chromatography (HPLC) analysis, a reference sample of syringin was used that met the requirements of pharmacopeial monograph VFS 42-2088-92. Methods of UV-spectroscopy and HPLC were used to determine the quantity of biologically active substances.Results and discussion. As a result of the research, other methodological and methodological approaches have been developed to determine the content of the total of phenylpropanoids in terms of eleutheroside B (syringin) by direct UV-spectrophotometry using a standard sample of syringin or the value of its specific absorbance. New conditions for HPLC analysis of eleutheroside B (syringin) in preparations of Eleutherococcus senticosus have been selected.Conclusion. As a result of the phytochemical study, a spectrophotometric method was developed for the determination of the total of phenylpropanoids in terms of syringin and an HPLC method for the determination of eleutheroside B (syringin) using an external standard after preliminary purification of the total extracts by filtration through a layer of aluminum oxide.

An integrated strategy for chemical, biological and palynological standardization of bee propolis

An integrated method for standardization of propolis using chromatographic, spectroscopic, palynological and biological analyses was implemented in this study. Digitally-enhanced HPTLC images using dual visualization approach with four image analysis software packages adopting different digitalization algorithms were comparatively evaluated i.e.; Sorbfil TLC View®, ImageJ®, JustTLC® and Gel Analyzer®. ImageJ® and Gel Analyzer® showed superior figures of merits in case of flavonoids and phenolic acids quantitation, respectively. Unsupervised and supervised multivariable pattern recognition models were constructed for comprehensive discrimination between the three propolis types (blue, orange and green) collected from various geographical locations worldwide. HPTLC-ESI-MS was utilized for precise identification of the discriminatory phytoconstituents. Fingerprint-efficacy relationship analysis was conducted via an Orthogonal Projection to Latent Structure multivariate model to unravel the bio-efficient markers in terms of α-glucosidase and α-amylase inhibition as main targets. 3,4-Dimethoxycinnamic acid, caffeic acid, isoferulic acid, rosmarinic acid, and quercetin were found to be the main health-relevant markers. A complementary fast, simple and readily available UV spectroscopic method using aluminium chloride as bathochromic shift reagent was used as an independent method for prediction of aforementioned biomarkers using a validated Partial Least Squares Regression model. In addition, palynological analysis was implemented to determine the botanical origin of Egyptian propolis for the first time. Twenty-eight pollen types assigned to 13 families were identified, with Asteraceae being the highest representative one. The investigated samples lacked dominant pollen types, which reflected the multifloral origin of Egyptian propolis. Identification of plant sources of propolis, which directly affect its chemical composition and subsequently its biological efficacy represents an integral part of its standardization.

Terpenoids from Litsea lancilimba Merr. and their chemotaxonomic significant

Phytochemical investigation into the fruits of Litsea lancilimba Merr. led to the isolation of eleven compounds, including six sesquiterpenoids (1–6), three monoterpenoids (7–9), and two norisoprenoids (10–11). The occurrence of compounds 2–3, 6–9 in Lauraceae family have been reported for the first time. The structures of these compounds were elucidated by comprehensive spectral methods, including UV, NMR, CD, optical rotation and other spectroscopy methods. The chemotaxonomic significance of isolated compounds was also elucidated.

Development and Validation of Prochlorperazine Maleate in Bulk and Pharmaceutical Dosage Form by UV Spectroscopic Method

Objectives: Prochlorperazine maleate is an antipsychotic and antiemetic drug that is available as tablets on the market for oral administration. The main object of this research work is to develop and validate the easiest UV spectroscopic method for the determination of Prochlorperazine maleate for tablet formulation (marketed formulation). Materials and Methods: A simple, reproducible and efficient method for the determination of Prochlorperazine maleate in bulk and tablet formulations have been developed. The developed method is based on the estimation by UVVisible spectroscopy. In this method, 0.3M HCL was selected as the solvent. A Wavelength selected for estimation of Prochlorperazine maleate was 254 nm. Results: Linearity was found in the concentration range 2-16μg/ml (R2=0.9909). A recovery study was found to be 98.01-99.79% for Prochlorperazine maleate. The method was found to be precise as % RSD was low in repeatability, intermediate precision, and reproducibility. LOD and LOQ were found to be 0.58 and 1.75 respectively. Conclusion: The method was found to be very simple, and precise. It also had a good recovery. The Developed method can be used for further routine analysis and stability study of a Prochlorperazine maleate.

Development and validation of the UV spectroscopic method for varenicline determination in pharmaceutical preparation

A simple and accurate UV visible spectroscopic method has been developed and validated for quantification of varenicline in bulk drug and pharmaceutical dosage forms. A shimadzu UV spectrophotometer was used considering availability and cost effectiveness of the proposed method. λmax of 236nm and 319nm were observed from the spectroscopic method. The calibration curve was linear (r = 0.99) in the studied range of concentration (5- 40 µg /ml) in both the absorption maxima. The selectivity and sensitivity of the elaborated method were satisfactory, and the limits of detection and quantification were less than 20% of the specification level. Moreover, the inter- and intra-day precisions was found to be less than 3% (RSD), while the recovery values expressing inter- and intra-day accuracy was varied from 99.73 to 101.23. The varenicline solution was stable over a period of 3 days on storage under refrigeration. The utility of the developed method was examined by analyzing the tablets containing VAR. As a result, the method was found to be selective, sensitive, precise and accurate.

Sensitivity Enhanced Ecofriendly UV Spectrophotometric Methods for Quality Control of Telmisartan and Benidipine Formulations: Comparison of Whiteness and Greenness with HPLC Methods.

The development of an environmentally friendly analytical technique for simultaneous measurement of medicines with large concentration differences is difficult yet critical for environmental protection. Hence, in this work, new manipulated UV-spectroscopic methods with high scaling factors were established for concurrent quantification of telmisartan (TEL) and benidipine (BEN) in fixed-dose combinations. Two different methods were developed and established by calculation of peak height at zero crossing point of second derivative and the ratio of first derivative spectra with a scaling factor of 200 and 100, respectively. The absorption difference between the peaks and troughs of the ratio spectra, as well as continuous subtraction from ratio spectra, were established as additional methods. In addition, new procedures were validated using ICH recommendations. The proposed methods’ linearity curves were constructed in the range of 0.5–10 µg mL−1 and 1–30 µg mL−1 for BEN and TEL, respectively, under optimized conditions. Furthermore, both the detection (0.088–0.139 µg mL−1 for BEN and 0.256–0.288 µg mL−1 for TEL) and quantification limits (0.293–0.465 µg mL−1 for BEN and 0.801–0.962 µg mL−1 for TEL) were adequate for quantifying both analytes in the formulation ratios. The accuracy and precision were confirmed by the good recovery percent (98.37%–100.6%), with low percent relative error (0.67%–1.70%) and less than 2 percent relative standard deviation, respectively. The specificity of the methods was proven by accurate and precise outcomes from the standard addition method and analysis of laboratory mixed solutions with large differences in concentrations of both analytes. Finally, the BEN and TEL content of the formulations was determined simultaneously without prior separation using these first ever reported spectroscopic methods. Furthermore, developed UV derivative spectroscopic methods demonstrated high greenness and whiteness when compared to the reported HPLC methods. These findings show that the projected methods were effective, practical, and environmentally acceptable for quality control of BEN and TEL in multicomponent formulations.

Evaluating the Influence of Deficit Irrigation on Fruit Yield and Quality Indices of Tomatoes Grown in Sandy Loam and Silty Loam Soils

The most important biotic stress factor impacting tomato crop biophysical, biochemical, physiological, and morphological features is water stress. A pot experiment was undertaken in a greenhouse to study the drought responsiveness of tomato (Solanum lycopersicum) yield and quality indices in sandy loam and silty loam soils. For both sandy loam and silty loam soils, the water supply levels were 70–100% FC, 60–70% FC, 50–60% FC, and 40–50% FC of ETo (crop evapotranspiration) from the vegetative stage to the fruit ripening stage, calculated using the Hargreaves–Samani (HS) model compared to the time-domain reflectometer (TDR) values calibrated using volumetric water content (VWC). The experiment was conducted as a 2 × 4 factorial experiment, arranged in a completely randomized block design, with four treatments replicated four times. In this study, we examined how sandy loam and silty loam soils at different % FC affect the total marketable yield and quality components of tomatoes, concentrating on total soluble solids (Brix), fruit firmness, dry fruit mass, pH, titratable acid (TA), ascorbic acid (Vit. C), and carotenoid composition. Lycopene and β-Carotene were estimated using the UV spectroscopy method, with absorption spectra bands centered at 451 nm, 472 nm, 485 nm, and 502 nm. The results revealed that even though there were some limitations, TDR-based soil moisture content values had a strong positive correlation with HS-based evapotranspiration, with R2 = 0.8, indicating an improvement whereby TDR can solely be used to estimate soil water content. Tomato plants subjected to 40–50% FC (ETo) water stress in both sandy loam and silty loam soils recorded the highest total soluble solids, titratable acidity, ascorbic acid content, and β-carotene content at an absorption peak of 482 nm, and lycopene content at an absorption peak of 472 nm, with lower fruit firmness, fruit juice content, and fruit juice pH, and a reduced marketable yield. Similarly, tomato plants subjected to 60–70% FC throughout the growing season achieved good fruit firmness, percent juice content, total soluble solids, titratable acidity, ascorbic acid content, and chlorophyll content (SPAD), with minimum fruit juice pH and high marketable yield in both soil textural types. It is concluded that subjecting tomato plants to 60–70% FC (ETo) has a constructive impact on the marketable yield quality indices of tomatoes.

Development and Validation of Novel UV Spectroscopy Method for the Estimation of L-Glutathione in Bulk and Formulation with Congo Red

A novel UV-Spectroscopy method was developed and validated for the estimation of L-glutathione in bulk and dosage form with congo red. The wavelength at which L-glutathione and congo red mixture product showed maximum absorption at 568nm in distilled water. The developed method range was found to be10-90µg/ml. The developed method validated for linearity, precision, range, limit of detection, the limit of quantification, robustness, specificity, system suitability. The regression equation was found to be y = 0.0045x + 0.121 with correlation coefficient R² = 0.9983. The limit of detection and the quantification limit was 5.05 µg/ml and 15.32 µg/ml respectively. The developed method was found to be linear, precise, robust, economical for the evaluation of L-glutathione in bulk and dosage form.