UV Absorption Technique Research Articles

Practical Applications of Analysis by the Ultraviolet Absorbance Method

Abstract Techniques and applications of analysis by ultraviolet absorbance methods are discussed. Such methods can often be applied in circumstances where there has not been time to establish procedures in advance of sampling. The UV absorption technique has been set up as a standard method for aniline, DDT, ethyl benzene, dinitrophenol, isophorone, phenol, pyridine and styrene, and as a check for other substances such as methylene iodide, p-dinitrosobenzene and TDI.

Uneven distribution of alpha-naphthyl acetate in tissues.

In the mouse ear in which esterases had been inactivated with warm formol the distribution of α-naphthyl acetate was studied by an UV absorption technique. The substrate was found to concentrate in structures containing lipids. The physicochemical properties of substrates used in enzyme chemistry seem to be such that it might prove difficult to find any substrate that would distribute evenly in tissues. So far it does not seem to have been ruled out that this influences enzyme reactions by some mass law effect.