Soil quality declines due to the conversion of natural vegetation into farmland and grazing areas. The response of soil properties to land use and land cover changes (LULC) exhibits both spatial and temporal variations. This study aimed to assess the effects of LULC changes on the physico-chemical properties of soil in the Wojic watershed. Soil samples were collected from natural forests, bushland, shrubland, and cultivated lands across three landforms of the watershed (upper, middle, and lower) to evaluate the physical and chemical properties of the soil associated with different LULC types. The LULC categories were compared using mean values and critical thresholds for selected physicochemical soil properties. Soil analysis was conducted using R software, employing one-way analysis of variance (ANOVA). A normality test was performed before the post hoc analysis, and Tukey’s honest significance difference (HSD) test was utilized for mean separation among the LULC types. Additionally, a normalized difference vegetation index (NDVI) was calculated for the years 1997 and 2017. A simple linear regression model was developed to estimate soil physico-chemical properties over the past 20 years, using laboratory soil parameters and the NDVI for 2017. The pH values showed a slight decrease in cultivated land (from 5.7 to 5.4), bushland (from 7 to 6.6), and shrubland (from 6.5 to 6.2) over the study period. The analysis indicated a declining trend in physico-chemical properties, attributed to changes in vegetation cover and management practices. Consequently, the government needs to enforce policies and regulations that promote effective land resource management and utilization, with particular emphasis on the proper management and conservation of forests, bushlands, and shrublands, as well as measures to prevent increased land resettlement.