Large nitrogen (N) losses during fertilization in agricultural production may result in energy wastage, soil and water contamination, and potentially influence crop development. Thus, with the help of a 15N-labeled tracer, we carried out a field monitoring analysis of NH3 emissions in a long-term (9-year) conservation tillage agroecosystem of Mollisols in northeast China, in order to determine whether a no-tillage regime and four levels of stover mulching (0%, 33%, 67%, and 100%), combined with urease and nitrification inhibitors, could improve fertilizer utilization efficiency in agricultural systems by reducing ammonia volatilization. Our results showed that in comparison with ridge tillage, no-tillage with stover mulching levels of 33%, 67%, and 100% significantly reduced NH3 emission rates and cumulative volatilization from 159.67 to 130.42 g N ha−1 and 15N-NH3 cumulative volatilization emission by 26% (on average). Furthermore, the application of urease and nitrification inhibitors significantly reduced 15N-NH3 volatilization levels from 1.19 to 0.98 g N ha−1. Our research results demonstrate that a long-term no-tillage regime and straw mulching can significantly reduce NH3 volatilization in fertilizers. Furthermore, when combined with the use of urease and nitrification inhibitors, these practices further enhance the reduction in NH3 volatilization. Although the volatilization of 15N-NH3 is minimally studied in Mollisols, these findings provide a solid foundation for improving fertilizer utilization efficiency, reducing crop production costs and mitigating subsequent environmental pollution.
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