Mixed land use has the advantages of promoting the economic and intensive utilization of land and improving the efficiency of land use, which can help alleviate the current urban problems and promote the sustainable development of cities. Existing studies have usually used quantitative indicators to reflect complex and diverse mixed land use situations, and the conclusions obtained usually cannot provide a basis for functional selection in mixed land use practices. Therefore, this study took Shenzhen as the study area to explore whether there are differences in the urban vibrancy enhancement among different mixed land use types. First, the block-scale mixed land use dataset of the study area was constructed. Second, the spatial distribution characteristics of the main functional types and urban vibrancy in the study area were explored. Finally, the impact of mixed land use types on urban vibrancy was explored by using a multiple linear regression model and setting land use type as the dummy variable. The results show that the number of mixed-function blocks in Shenzhen is relatively small, and the mixed land use degree still needs to be improved. Among the 12 main land use types in the study area, those containing industrial land are usually clustered in the northern industrial area of Shenzhen, those containing public or commercial service land are usually clustered in the city center, and those containing residential land are widely distributed in the study area. From the perspective of urban vibrancy, there is a phenomenon of “jobs–housing mismatch” in Shenzhen, as well as a problem of low urban vibrancy in the peripheral areas of the city. In addition, the urban vibrancy intensity of mixed land use types including residential or commercial land is higher, such as “administration+residential”, “residential+commercial”, “industrial+residential+commercial”, and “administration+residential+commercial” land, which includes residential or commercial land, is stronger, while. However, the urban vibrancy stability of mixed land use types including industrial land is higher, such as “industrial+residential” and “industrial+administration” land. The results of this study can provide a basis for future mixed land use practices in terms of land use type selection. For the urban central areas and subcenters in urban peripheral areas, mixed land use types such as “administration+residential”, “residential+commercial”, and “administration+residential+commercial” can be selected to enhance the urban vibrancy stability of the area. For industrial parks in urban peripheral areas, mixed land use types such as “industrial+residential”, “industrial+commercial”, “industrial+administration+residential”, and “administration+residential+commercial” can be selected to enhance the urban vibrancy intensity of the area.
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