Using Ultrasound-assisted Extraction Research Articles

Antioxidant Activities of Ethanolic Extracts Obtained from α-Pinene-Containing Plants and Their Use in Cosmetic Emulsions

α-Pinene is the bicyclic, unsaturated terpene hydrocarbon present in many plants. Due to its beneficial chemical properties, this compound is of great interest and has found numerous applications as a raw material in many chemical industries as well as in medicine and cosmetics. The aim of this study was to evaluate the antioxidant activities of ethanolic extracts obtained from plants containing α-pinene and to test the properties of cosmetic emulsions prepared with these extracts. The raw plant materials consisted of fresh parts of Pinus sylvestris L., such as cones, needles, and branches, as well as dried unground and ground pinecones; dried and fresh Rosmarinus officinalis leaves; dried Levisticum officinale leaves; and dried Salvia officinalis L. leaves. The plant materials were individually extracted with 40% (v/v), 70% (v/v), and 96% (v/v) ethanol using ultrasound-assisted extraction (UAE) for 15, 30, or 60 min. This method is a green extraction technique, frequently applied to isolate active substances from plants. For the selected plant materials, Soxhlet extraction with 96% (v/v) ethanol was also performed. The qualitative and quantitative analyses of the components in the selected extracts were performed with gas chromatography coupled with mass spectrometry (GC-MS). The antioxidant activities of the extracts were evaluated with the DPPH and ABTS methods. The extracts of three plant materials with the highest antioxidant activities—dried Rosmarinus officinalis leaves, dried Salvia officinalis L. leaves, and dried and ground Pinus sylvestris L. cones—were selected to be incorporated in cosmetic emulsions containing glyceryl monostearate and Olivem 1000 as emulsifiers. The stabilities and antioxidant activities of the emulsions were evaluated. Moreover, the antimicrobial properties of the emulsions using microbiological tests were also determined. The findings suggest that the prepared emulsions are stable cosmetic products with a high antioxidant potential.

Uncovering the Nutraceutical and Biorefinery applications from two different Marine macroalgae Sargassum polycystum and Rosenvingea intricata

The marine ecosystem encompasses resources for various food products and nutraceuticals with multiple human health benefits. In this study, two underexploited seaweeds such as Sargassum polycystum (Sp) and Rosenvingea intricata (Ri) were used to investigate their biological potential. The seaweeds underwent for washing and the left washed water and washed biomass were examined for bioactive compounds using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The fucoxanthin was extracted from both seaweeds using Ultrasound-assisted extraction (UAE) with 100% absolute ethanol and acetone and resulted the higher fucoxanthin in Ri (2.42 ± 1.8 mg/g) than the Sp (1.8 ± 0.4 mg/g) . Further, the remnant seaweed biomass of both washed and unwashed forms was used for fucoidan extraction by employing UAE and microwave-assisted extraction (MAE). Among this, the MAE process had increased the fucoidan yield up to 2-fold in unwashed seaweed (4.75%) compared to the washed form (1.6%), which was higher than the UAE process. Finally, the relic seaweed residue of Sp underwent separate hydrolysis and batch fermentation (SHF) to produce bioethanol and was quantified by GC-MS analysis. Thus, this study suggests that the entire biomass of seaweed would be a plausible feedstock for producing bioproducts using the zero-waste concept.

Eco-friendly and high-performance extraction of flavonoids from lemon peel wastes by applying ultrasound-assisted extraction and eutectic solvents

This work aimed to develop an optimized extraction process to achieve an environmentally friendly proposal to recover flavonoids from lemon peel wastes using ultrasound-assisted extraction (UAE) and eutectic mixtures as solvents. The eutectic mixture choline chloride:acetic acid (ChCL:AA; 1:2 mol/mol) displayed the best extraction performance of the target compounds, here represented by hesperidin and narirutin. In Central Composite Rotatable Design (CCRD) approach, we identified the ideal conditions for flavonoid recovery: a solvent-to-feed ratio (R(S/F)) of 30 (v/m), 40% water content (m/m), and a static time of 40 min. Combined with a dynamic extraction time of 6 min (under cavitation), those parameters yielded optimal results in flavonoid recovery. The 320 W ultrasound power achieved the maximum yield of the target compounds (5.25 mghesperidin/gbiomass and 0.21 mgnarirutin/gbiomass). Besides, our results showed that the ChCL:AA solvent enhanced the thermal stability of flavonoids, showcasing remarkable potential as a stabilizing agent for various applications. Ultrasound-assisted extractions were superior in extraction performance compared to conventional techniques, namely maceration and magnetic stirring.

Green Extraction of Oleoresin from Pink Pepper Fruits: Effect of Experimental Conditions and Characterization

This work evaluated the green extraction of oleoresin from pink pepper fruits (ORPPF) using ultrasound-assisted extraction (UAE) and ethanol as a solvent. The effects of temperature, ultrasound power intensity, sample to solvent ratio and time on the global extraction yield (YGE) and phenolic compounds yield (YPC) were evaluated. The oleoresin samples were characterized and its antimicrobial activity determined, and the obtained results were compared to conventional extraction in Soxhlet. From the results it was found that the application of the highest levels of the independent variables favored the extraction process. The maximum values of YGE and YPC were 28.60 wt% and 6.37 mg GAE per g fruit, respectively, obtained at 60 °C, 100% of ultrasound power (165 W), 1:20 g mL−1 (sample:solvent) and 45 min. Under maximized conditions, the ORPPF obtained by UAE showed a content of phenolic compounds and antioxidant activity inferior to soxhlet–ethanol extraction. However, the time and solvent consumption were reduced. Oleic and linoleic acids predominated in the fatty acid composition of ORPPF, in addition to sesquiterpenes and gallic and syringic acids. The ORPPF presented weak antibacterial activity, with minimum inhibitory concentration ranging from 31.25 to 125 mg mL−1.

Antioxidant Activity, Alpha-Glucosidase Inhibitors Ethanol Extract of Vernonia amygdalina Del. and Nutraceutical Potential

Vernonia amygdalina Del is a shrub that is very well known in African continent and usually grows in tropical climates, including in Indonesia. In this research, we tested the The ethanol extract's anti-oxidant properties of Vernonia amygdalina Del leaves and the inhibitory activity of alpha-glucosidase. Vernonia amygdalina Del leaves ethanol extract was obtained using Ultrasound Assisted Extraction (UAE) method by variation of time and amplitude. Antioxidant activity of Vernonia amygdalina Del leaves ethanol extract was tested using DPPH and CUPRAC methods. Based on the research, IC50 of Vernonia amygdalina Del leaves ethanol extract was obtained by DPPH method respectively that is A (111.72±0.10)mg/L, B (154.08±0.02)mg/L, C (191.22±0.04) mg/L and D (190.99±0.08) mg/L. While IC50 of the Vernonia amygdalina Del leaves ethanol extract by CUPRAC method are A (26.71±4.85), B (78.57±5.04), C (43.50±4.62) and D (76.60±1.79). In this study, an alpha-glucosidase inhibition test was carried out where the greatest inhibitory activity was obtained by the Vernonia amygdalina Del leaves ethanol extract with variation A (30 minute extraction time with a 0.60 m amplitude) and IC50 is (0.69±0.13)mg/L. Based on this study, it can be concluded that Vernonia amygdalina Del has potential as an antidiabetic and nutraceutical drug.

Yanıt yüzey yöntemi kullanılarak Ziziphus jujube Mill yapraklarından fenolik bileşenlerin ultrason destekli ekstraksiyonunun optimizasyonu

The phenolic compounds in jujube (Ziziphus jujuba Mill) leaf were extracted using ultrasound assisted extraction (UAE) for potential of antioxidant food additive in this study. The extraction factors such as methanol concentration, temperature and time were optimized using response surface methodology (RSM) to maximize of the total phenolic content (TPC), 2,2 -diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and phenolic profile in jujube leaf extracts (JE). The best possible range for methanol concentration (25-50%), duration of ultrasound (20-40 min), ultrasonic temperature (40–60 °C) were obtained using the Box-Behnken design (BBD). The optimum extraction parameters were obtained with 25% methanol concentration, 20 min duration of ultrasound and 49.89 °C ultrasonic temperature. Ellagic, caffeic, rosmarinic acid, and rutin were determined as major phenolics in JE under optimal extraction parameters. The results revealed that UAE is an effective pretreatment for extracting bioactive ingredients from JE as potential functional food additive. Furthermore, RSM is an effective method for optimizing the UAE factors.

Comparative extraction and antioxidant potential of bioactive compounds from Fucus vesiculosus: Kinetic modeling and UPLC-Q-TOF-MS phenolic profiling

This study explores efficient bioactive compounds extraction from Fucus vesiculosus (FV) seaweed using Ultrasound-assisted extraction (UAE) and Solid Liquid Extraction (SLE). Varied ultrasound intensities (UI) (58.8, 103.1 and 164.5 kW m−2 for U1, U2 and U3, respectively) were employed, and Peleg's model simulated extraction kinetics, revealing UAE's remarkable 10-min equilibrium contrasted with SLE's 3 h, showcasing UAE's time efficiency. UAE, particularly at U3, yielded significantly higher Total Phenolic Content (TPC) and Uronic Acid Content (UAC) compared to SLE, reaching maximum concentrations of 2.26 ± 0.01 g PGE 100 g−1 DFV and 7.87 ± 0.11 g GAE 100 g−1, respectively. Increased UI enhanced DPPH and ABTS antioxidant activities, while low UI resulted in higher FRAP values. After 20 min of extraction, U3 demonstrated superior DPPH scavenging activity (5.75 ± 0.23 μM TE g−1 DFV), surpassing that of SLE after 20 h of extraction (4.76 ± 0.11 μM TE g−1 DFV). Additionally, U3 displayed elevated ABTS values (8.92 ± 0.33 μM TE g−1 DFV) compared to 6.46 ± 0.41 μM TE g−1 DFV for SLE, with no significant difference observed in FRAP values. These results highlight U3's enhanced antioxidant potential in comparison to SLE. The chromatographic analysis identified six main compounds through UPLC-Q-TOF-MS and HPLC. Higher concentrations of phloroglucinol, gallic acid, catechin, protocatechuic acid, and chlorogenic acid were observed in U3. However, SLE exhibited a higher ferulic acid content. Future research aims to explore phloroglucinol derivatives and FC, contributing to FV' potential applications.

Extraction of Flavonoid Compounds from Red Betel Leaves (Piper Crocatum) Using Ultrasound-Assisted Extraction (UAE) Method

Red betel leaf is one of the medicinal plants known for its beneficial antioxidant content in maintaining overall health. One of the types of antioxidants found in red betel leaf is flavonoids. In this study, the extraction of red betel leaf was conducted using the Ultrasound-Assisted Extraction (UAE) method with the aim of analyzing the flavonoid content and antioxidant activity in the red betel leaf. A 90% ethanol solvent was used, and the extraction variables included the ratio of material to solvent, particle size, and extraction time, which were determined using Design Expert13 software. The flavonoid test was performed using a UV-Vis spectrophotometer at a wavelength of 431 nm. The analysis of antioxidant activity was conducted using DPPH (2,2-Diphenyl-1-picrylhydrazyl). The highest flavonoid content obtained was 263.676 mgQE/g under the optimum operating conditions with a particle size of 100 mesh, a material-to-solvent ratio of 0.06, and an extraction time of 25 minutes.

Characterization of ‘novel fucoidan’ extracted from brown seaweed (Sargassum echinocarpum J. Ag.) using Ultrasound-Assisted Extraction (UAE) and its potential antioxidant activity

Fucoidan of Sargassum echinocarpum was extracted using the UAE method. This method produced a fucoidan extract with a yield of 2.82-3.93% and antioxidant activity of 44.57-98.77 ppm at different temperatures and extraction times. The total sulphate, fucose, phenolic, protein, and uronic acid contents were 5.50-6.24%, 17.22-63.22%, 14.66-78.20 mgGAE/g, 0-14.42 mg/g, and 191.43-353.76 mg/g, respectively. Furthermore, extraction treatment with UAE also affected the topology and morphology of the fucoidan extract. The analysis results showed the presence of monosaccharides such as galactose 1.37%, mannose 0.642%, and xylose 2.86%, while glucose and rhamnose were not detected. The extract also had the same absorption band as the fucose and fucoidan standard (F. vesiculosus) as well as a similar basic skeletal structure. Based on the experimental results, the fucoidan extract had excellent antioxidant properties.

ULTRASOUND-ASSISTED EXTRACTION OF STARCH FROM OIL PALM TRUNK: AN OPTIMIZATION BY RESPONSE SURFACE METHODOLOGY

Higher production of palm oil in Malaysia has resulted in a significant amount of oil palm biomass including oil palm trunk (OPT). OPT has been studied as an alternative starch source. To date, reports on the extraction of starch from OPT using ultrasound-assisted extraction (UAE) are limited. The application of UAE is considered to be a simpler and effective way in starch extraction compared to conventional extraction methods. This study aims to investigate the potential of ultrasound-assisted extraction to extract starch from OPT, and optimization using response surface methodology. A three-factor central composite design (CCD) was designed to determine the effects of variables on response. The three variables involved in the study, namely: sonication temperature (45-65°C), sonication time (30-60 min) and sodium metabisulfite concentration (0.5-1.5% w/v). All of the data were analysed using Analysis of Variance (ANOVA). The R2 value (95.6%) and model (p <0.0001), indicating the acceptability of the model and significance in the interaction between independent variables. At the optimum conditions of 52.84°C temperature, 30.66 min exposure time and 0.58% w/v sodium metabisulfite concentration, the starch yield achieved its optimum value by 34.21%. Therefore, this study proved the potential of UAE in extracting higher OPT starch.

Assessment of Cd, Cu, Pb, and Zn concentrations in leather by ICP-MS: a multivariate analysis of an eco-friendly approach

ABSTRACT Due to the metal-complex dyes used in tannery industries, potentially toxic metals can be present in leather. However, publications regarding metal determination in leather are scarce, and most sample preparation procedures are still time-consuming. Therefore, a simple and straightforward sample preparation approach using ultrasound-assisted extraction (UAE) was proposed for the determination of Cd, Cu, Pb and Zn in leather samples by ICP-MS. A full factorial design 23 was used to investigate the extraction conditions. Fitted conditions were obtained at a ratio of 100 mg: 1.0 mL (sample:HNO3 14.0 mol L−1), ultrasound-assisted extraction time of 55 min at 75 ± 3°C. The method´s accuracy was evaluated through the comparison of results with those obtained using microwave-assisted digestion (MWAD) approach. The UAE results were statistically similar to the MWAD, according to the Student t-test (α = 0.05) and linear regression analysis. Limits of detection (LOD), at mg kg−1 range, and method precision (RSD <11%) were suitable for the analytes´ determination in leather. The proposed method was successfully applied to analyse 18 different leather samples. The results were < 0.3 to 53.19 mg kg−1 for Cd, <0.2 to 484.61 mg kg−1 for Cu, <0.02 to 211.31 mg kg−1 for Pb and < 0.02 to 379.22 mg kg−1 for Zn, respectively. The proposed method is simple and faster than the traditional acid digestion sample preparation. Moreover, according to green analytical chemistry, the method scored 82 points on the Eco-Scale, confirming the proposed method as environmentally friendly.

Green Processing of Black Raspberry Pomace: Application of Sonotrode-Based Extraction Technique and Particles from Gas-Saturated Solutions (PGSS) Technology.

The aim of this study was to develop, for the first time, anthocyanin-enriched fractions from black raspberry pomace (BRP) using ultrasound-assisted extraction (UAE) via sonotrode and the Particles from Gas-Saturated Solutions (PGSS) process. UAEs with different amplitudes and sonication times were evaluated and showed relevant effects on the yields of target analytes. The raspberry pomace extracts were formulated in a powder form by PGSS using glyceryl monostearate as a carrier at different extract-to-carrier ratios of 1:11, 1:5, and 1:3. The effects of all variables were evaluated in terms of extraction yield, total phenolic content, and encapsulation yield. UAE was strongly affected by amplitude, and the highest amplitude (100%) provided the best results for extraction yield and total phenolics. HPLC of UAE extracts and powders was utilized for quantification of polyphenol compounds, showing cyanidin-3-rutinoside as a main compound, followed by cyanidin-3-glucoside, rutin, ellagic acid, and gallic acid. These results show that these time-efficient and high-performance techniques enable the production of natural fractions from industrial BRP with acceptable characteristics to be used for the development of nutraceuticals and different food formulations.

Rice bran stabilization using autoclave and optimization of crude rice bran oil recovery using ultrasound-assisted extraction

This research aimed to stabilize rice bran to reduce enzymatic reactions and optimize rice bran oil extraction using ultrasound-assisted extraction (UAE). The optimization was performed using response surface methodology (RSM). The results showed that rice bran oil has three primary fatty acids (oleic, linoleic, palmitic). Stabilization of rice bran using an autoclave (121 °C) for 10 min was recommended, with oil yield (14.2%), free fatty acid/FFA (0.41%), and gamma oryzanol (16.1 ppm). The storage significantly affected oil yield and gamma oryzanol but not FFA (p&gt;0.05). The untreated and stabilized rice bran FFA during 18-day storage ranged from 1.33 to 5.73% and 0.41 to 1.12%. This confirmed that stabilization could inactivate lipase enzymes by reducing FFA. The optimization showed that the linear model best explained oil yield, total phenol content (TPC), and antioxidant activity (IC50), while the 2FI model was best fitted for gamma oryzanol. The optimal condition was found for UAE extraction at an amplitude of 90% and a time of 30 min. Verifying optimal conditions resulted in oil yield (13.6%), TPC (44.8 mg GAE/g), IC50 (207.2 ppm), and gamma oryzanol (15.8 ppm). Further in-depth studies are required to investigate using green solvents in the UAE and gamma oryzanol purification.

Supercritical CO2 and ultrasound extraction and characterization of lipids and polyphenols from Phacelia tanacetifolia Benth. pollen

The phytochemical profile of Phacelia tanacetifolia Benth. pollen was characterized in terms of phenolic compounds and fatty acids. Fatty acids were extracted from pollen using supercritical CO2 and characterized by GC-FID. The main constituents found in the extract were palmitic and oleic acid accounting for 50% of all fatty acids. The polyphenols were extracted from exhausted material after CO2 extraction using ultrasound-assisted extraction (UAE) and aqueous methanol solution as solvent. The major compounds identified in the UAE extract by UHPLC-DAD-QqTOF-MS belonged to flavonoid glycosides (isorhamnetin O-rutinoside-rhamnoside) and phenolamides (di-p-coumaroylputrescine, N′,N''-di-p-coumaroyl-N'''-caffeoylspermidine, and N′,N'',N'''-tri-p-coumaroylspermidine isomers). The UAE extraction of flavonoids and phenolamides was optimized considering time, temperature, methanol percentage and solvent-solid ratio. The determined optimized conditions were: temperature of 66.1 °C, time of 44.9 min, solvent-solid ratio of 49.9 mL/g and 70.1% of aqueous methanol solution as a solvent. The statistically significant impact on the extraction of the analyzed compounds had the solvent-solid ratio and methanol percentage as well as interactions between those two parameters. The optimized conditions may be useful for efficient extraction of fractions rich in bioactive compounds e.g. N′,N'',N'''-tri-p-coumaroylspermidine with proven biological potential.

Metabolite profiling of anticancer compounds in Saussure lappa based on UPLC-QToFMS/MS

Background: Saussure lappa is a plant of the Asteraceae family that has been known as a medicinal plant to treat cancer. However, there is still a lack of data about the metabolite profiling of this extract and target activity in the cancer signaling pathway. Objective: This study aimed to identify metabolites and evaluate the anticancer activity of Saussure lappa active ingredients. Method: The root sample was extracted using Ultrasound-Assisted Extraction (UAE) with 96% ethanol solvent. Evaluation of the anticancer activity of the compounds was carried out using the PASS SERVER tools. Result: About 17 metabolites have been characterised. Interestingly, Saussureamine B, C, D, costunolide, and dehydrocostus lactone showed highly antineoplastic potential properties with several possible activities such as apoptosis agonists, MMP9 expression inhibitors, and Transcription factor NF-kappa B inhibitors. Conclusion: The root extract of Saussure lappa is highly recommended to be developed as an anticancer drug candidate.

Optimization of ultrasonic-assisted extraction of Platycodon grandiflorum polysaccharides and evaluation of its structural, antioxidant and hypoglycemic activity

The study aimed to improve the extraction rate of Platycodon grandiflorum roots polysaccharides (PGPs) using ultrasound-assisted extraction (UAE). A comparative analysis was undertaken to evaluate polysaccharides content, molecular weight distribution, monosaccharide composition, preliminary structure, antioxidant, and hypoglycemic activity of UAE in comparison with heating water extraction (HWE). The optimum extraction conditions included a liquid-to-material ratio of 20 mL/g, ultrasonic power of 150 W, extraction temperature of 70 ℃, and extraction time of 20 min, resulting in a significantly greater polysaccharides (12.011 ± 0.91 %) compared to HWE (7.62 ± 0.18 %). Through Sephacryl S-100 column elution, two homogenous fraction (PGP-U extracted with UAE and PGP-H extracted with HAE) were obtained.The molecular weight of PGP-U and PGP-H was 3.14 kDa and 3.44 kDa, respectively, mainly composed of different proportions of fourteen monosaccharides. Fourier transform infrared spectroscopy (FT-IR) and Nuclear Magnetic Resonance (NMR) spectra experiment results showed that the two polysaccharides were pyranose ring with α- and β-glycoside bond. PGP-U and PGP-H exhibited specific antioxidant activities, encompassing total reducing force, scavenging of DPPH radicals, ABTs radicals and hydroxyl radicals in vitro, along with mitigation of H2O2-induced damage in HepG2 cells. Moreover, PGP-U exerted significantly stronger inhibitory activities against α-amylase and α-glucosidase and could significantly enhances the glucose uptake capacity and intracellular glycogen content of insulin-resistant HepG2 (IR-HepG2) cells.

Exploration of In-vitro Antidiabetic Activity of ZnO NPs and Ag NPs Synthesized using Methanolic Extracts of Alpinia mutica and Tradescantia spathaeca Leaves

Diabetes might be cured with the use of medicinal herbs and environmentally friendly production of metallic nanoparticles (Ag NPs) and ZnO NPs. The methanolic leaf extracts of Alpinia mutica and Tradescantia spathaeca were used to synthesize silver nanoparticles (Ag NPs) and zinc oxide nanoparticles (ZnO NPs), respectively, for in-vitro evaluation. Methanolic leaf extracts of A. mutica and T. spathaeca were used to create AgNPs and ZnO NPs under ambient conditions using ultrasound-assisted extraction (UAE). Their ability to block alpha- and beta-amylase confirmed the in-vitro antidiabetic efficacy of methanolic leaf extract of plant (MLEP), AgNPs, and ZnO NPs. In this study, α- amylase activity of ZnO and nanoparticles of silver produced from natural sources will be evaluated in an effort to lessen the toxicity and negative effects of the inhibitor used to treat diabetes. Antidiabetic action was especially impressive in the ZnO and silver nanoparticles produced using methanolic extracts of A. mutica and T. spathaeca. Because of their promising in-vitro antidiabetic action with alpha-amylase activity, MLEP of A. mutica and T. spathaeca, AgNPs, and ZnO NPs show promise for future medical uses.

New selective and sustainable ultrasound-assisted extraction procedure to recover carnosic and rosmarinic acids from Rosmarinus officinalis by sequential use of bio-based solvents

Carnosic (CA) and rosmarinic (RA) acids are the primary phenolic acids in hydrophilic rosemary extracts. Their combination exhibits high antioxidant activity and can be explored in several applications. This study aimed to develop an extraction procedure using bio-based solvents to recover two rosemary extracts, one rich in CA and the other in RA. By using ultrasound-assisted extraction (UAE) and a pool of 34 solvents, we evaluated nominal power (W), extraction time (min), and solvent water percentage (% H2O) regarding yield and selectivity. The authors propose a sequential UAE procedure validated by applying ethanol 99.5 % (v/v), 240 W, and 5 min to recover a rich fraction of 24.0 mgCA.gbiomass−1; followed by a second step using AmAc:LA (1:2 M ratio), 20 % H2O (m/m), 320 W, and 5 min that resulted in 8.4 mgRA.gbiomass−1. Our results indicate that modulating the solvent composition and process temperature is critical to increasing extraction yields and selectivity.

Optimisation of ultrasound-assisted extraction of carotenoids and antioxidant activity from Citrus reticulata Blanco peels (Wilking mandarin)

AbstractA large amount of waste, especially the outer part of citrus fruits (peel), is generated after consuming the pulp and it remains unused. The valorisation of this waste by recovering its bioactive compounds seems interesting. The aim of this study was to find the optimal conditions using ultrasound-assisted extraction (UAE) that yield the highest carotenoid content and better antioxidant activity from Citrus reticulata Blanco peels.Response surface methodology (RSM) through Box–Behnken experimental design was used to optimise the conditions for carotenoid extraction using UAE. Hexane concentration, temperature, and sonication time were selected as the main factors.The results revealed that all independent variables affected the responses. The optimal UAE conditions for hexane concentration, temperature, and sonication time were 60.76%, 36.45 °C, and 37.32 min, respectively. The values of total carotenoid content (TCC) and total antioxidant activity (TAA) obtained by UAE were higher than those obtained by the maceration extraction method.It can be concluded that the medium and extraction parameters, including hexane concentration, temperature, and sonication time, significantly influenced the recovery of carotenoids and antioxidant activity. The optimisation study allowed determining the appropriate conditions to maximise both responses. Compared to conventional maceration, the UAE method was superior and more efficient for extracting carotenoids from C. reticulata Blanco peels.

Edible Oil sebagai Pelarut Ekstraksi Karotenoid dari Kulit Labu Kuning (Cucurbita moschata)

Carotenoids are pigments found in various types of fruits and vegetables that provide yellow, orange, and red colors. This study aimed to extract yellow pumpkin skin using edible oil solvents. The extraction process was carried out using ultrasound-assisted extraction (UAE) method with virgin coconut oil (VCO) and palm kernel oil (PKO). Yellow pumpkin peel powder with sizes of 40 mesh and 100 mesh were extracted with variations in extraction time (30 minutes, 60 minutes, and 90 minutes) and variations in solid-to-solvent ratio (1:10, 1:20, 1:30, 1:40, and 1:50). UV-Vis spectrophotometer analysis was used to determine the concentration of the extraction results. According to the research results, the best carotenoid concentration of 2513.44 ppm was obtained using PKO solvent with a powder size of 100 mesh, extraction time of 30 minutes, and powder-to-solvent ratio of 1:10.