Abstract
The phytochemical profile of Phacelia tanacetifolia Benth. pollen was characterized in terms of phenolic compounds and fatty acids. Fatty acids were extracted from pollen using supercritical CO2 and characterized by GC-FID. The main constituents found in the extract were palmitic and oleic acid accounting for 50% of all fatty acids. The polyphenols were extracted from exhausted material after CO2 extraction using ultrasound-assisted extraction (UAE) and aqueous methanol solution as solvent. The major compounds identified in the UAE extract by UHPLC-DAD-QqTOF-MS belonged to flavonoid glycosides (isorhamnetin O-rutinoside-rhamnoside) and phenolamides (di-p-coumaroylputrescine, N′,N''-di-p-coumaroyl-N'''-caffeoylspermidine, and N′,N'',N'''-tri-p-coumaroylspermidine isomers). The UAE extraction of flavonoids and phenolamides was optimized considering time, temperature, methanol percentage and solvent-solid ratio. The determined optimized conditions were: temperature of 66.1 °C, time of 44.9 min, solvent-solid ratio of 49.9 mL/g and 70.1% of aqueous methanol solution as a solvent. The statistically significant impact on the extraction of the analyzed compounds had the solvent-solid ratio and methanol percentage as well as interactions between those two parameters. The optimized conditions may be useful for efficient extraction of fractions rich in bioactive compounds e.g. N′,N'',N'''-tri-p-coumaroylspermidine with proven biological potential.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call