Use Of Tissue Preparations Research Articles

History of development tissue therapy and modern prespectives of it’s application in veterinary medicine

Medical products, made of plants, various tissue substrates of animals and humans, have been used for a long time in human and veterinary medicine. Tissue medications of animal origin have been widely used in veterinary science both for prophylactic and therapeutic purposes and as growth factors for fattening of all species. The development of modern biology and some fields of it, human and veterinary medicine in particular, based on the achievements of advanced technologies, provides an opportunity for the appearance of new effective medications in clinical practice. Over time, after the reorganization of the agricultural sector and veterinary medicine as one of its main divisions, tissue medications began to disappear from the range of medical preparations, and they have been almost forgotten and basically not used in recent years. Veterinary medicines market has become overwhelmed with mainly imported synthetic medications and antibiotics. Meanwhile, clinical practice is experiencing an intensive introduction of medications, produced by private factories, which are combinations and mixtures of various existing pharmacological substances. Their annotations indicate a universal and beneficial effect on the body of almost all domestic animals, birds and bees. The paper is aimed at the feasibility study and the restoration necessity of production and use of tissue preparations in veterinary clinical practice. The materials used for the research are the reports published in scientific journals, collections of scientific editions of universities and research establishments, textbooks, newspapers, etc. We have become the first to create tissue medications such as fetoplacentat, made from the uterus and its contents of different pregnancy term of clinically healthy cows, pigs, mares, dogs and cats, and truthenate, made from the larvae of drones aged 5–7 days, for their application in eterinary medicine, obstetrics and gynecology in particular. Production trials of medications carried out on different pregnant animals, have made it possible to identify their corrective effect on the course of pregnancy, childbirth and postpartum period, high preventive activity in the development of functional disorders, subinvolution and inflammatory processes of the genital organs, as well as in the treatment of different forms of infertility in animals and for the improvement of spermatogenesis of male breeders. Further research will focus on the development and implementation of effective methods and measures aimed at elimination of infertility and improvement of the reproduction of farming animals on condition of the use of tissue preparations.

The use of the drug «Aminoton», aimed at prevention of complications of postpartum sows

Application of tissue preparation «Aminoton» clinically healthy sows at the beginning puerperale period caused a pronounced preventive effect. So the above animals has improved the functioning of the hematopoietic system (increased compared to control group animals, the levels of hemoglobin and red blood cells), there was normalization of metabolic processes (increased compared to benchmarks, total protein, albumin). The use of the drug «Aminoton» clinically healthy animals in the postpartum period increased compared to control antibacterial, lysozyme and complementary activity of blood serum and enhanced the nonspecific immune defense of the body. The use of «Aminoton» sows with inflammatory complications in the puerperal period have greatly improved their clinical and physiological condition. In the same dysfunctional animals, which did not use the above-mentioned pharmacological agents in puerperale the period from the beginning of the inflammatory process dynamics of the main clinical and physiological, hematological, biochemical and humoral indicators over time, deteriorated. In a dysfunctional untreated pigs at 21 days after birth increased compared to sick animals treated by this drug «Aminoton» the number of leukocytes, increased content of total protein in serum C-reactive protein and seromucoid that had severe signs of inflammation in the body. Patients with untreated pigs 21 days after delivery significantly increased in serum compared with patients treated «Aminoton» animals, the activity of alanine aminotransferase, alkaline phosphatase content of β-lipoproteins, sialic acids, decreased glucose levels, indicating the possibility of substance-induced load and liver damage, and increased activity of Aspartate aminotransferase be a sign of disorders of metabolic processes in heart muscle. The use of the drug «Aminoton» animals with inflammatory processes immediately after birth improved dynamics of indicators of nonspecific immunity. Sows in the treated with inflammation of the reproductive organs at 21 days after birth increased in comparison with patients with untreated animals bactericidal, lysozyme and complementary activity of blood serum. For early diagnosis and prognosis of inflammatory processes in the organs of the reproductive system of sows in postpartum period, we offer you to consider rates of precursors for a birth and according to the diagnosis of conduct for prevention of postpartum pathology in animals with the use of tissue preparation «Aminoton» in a dose of 0.05 ml per 1 kg of live weight in the first 2 hours after birth and 48 hours.

Isoguvacine binding, uptake, and release: relation to the GABA system.

Isoguvacine (1,2,3,6-tetrahydropyridine-4-carboxylic acid) is a GABA (gamma-aminobutyric acid) agonist with limited conformational flexibility. In these studies we investigated the binding, uptake, and release of [3H] isoguvacine by use of tissue preparations of rat CNS, comparing the results with similar studies of [3H]GABA. The results from these investigations indicate that isoguvacine binds to membrane preparations of rat forebrain with pharmacological characteristics similar to the post-synaptic GABA recognition site; that it is transported into synaptosomal preparations by an uptake system similar to the high-affinity GABA uptake system; and that recently accumulated isoguvacine is released in a Ca2+-dependent manner and by heteroexchange with external GABA. The ability of isoguvacine and gamma-hydroxybutyric acid to decrease the K+-stimulated Ca2+-dependent release process was also investigated. The results indicate that isoguvacine interactions have many of the biochemical features of GABA synaptic function, isoguvacine being, however, less potent than GABA.

The hydrolysis of flavouring esters by artificial gastrointestinal juices and rat tissue preparations

The rates of hydrolysis of sixteen esters used as constituents of artificial flavours have been determined in artificial gastrointestinal juices and in fresh preparations of rat liver and small intestine. The artificial gastrointestinal juices exhibited a limited ability to hydrolyse the esters and of which methyl anthranilate was virtually unaffected. On the other hand, rat liver and small intestinal preparations were found readily to hydrolyse the esters to their component acids and alcohols. These findings showing large differences between the results obtained from the two methods suggest that use of tissue preparations is likely to produce data more relevant to the toxicological assessment of esters.