Use Of Coconut Water Research Articles

Optimalisasi Fosfat untuk Meningkatkan Pertumbuhan Kerapatan Populasi dan Kemampuan Antagonis Saccharomyces cerevisiae terhadap Fusarium sp.

Saccharomyces cerevisiae is a common yeast used as a fermenter in the home industry. This yeast is able to grow in media like waste materials. One of the waste materials that can be used as a medium of yeast growth is waste of coconut water. The use of coconut water as a medium of yeast propagation has been widely used in some types of yeasts. The intake of nutrients such as phosphate will make the yeast cells begin to grow and work faster. The yeast cell takes phosphate as ATP. Khamir will turn it into a phosphate polymerization form that is often found within the mitochondria of these cells. S. cerevisiae has the ability not only in terms of fermentation but also can perform other functions in the biological control process. The main methods of this study include the growth test of S. cerevisiae with the addition of a phosphate (KH2PO4), S. cerevisiae growth test by aerator method, yeast antagonist test. The results showed that S. cerevisiae was able to grow higher with the addition of phosphate nutrients (0.5% KH2PO4). This yeast has the potential to control Fusarium sp. The percentage of inhibition was isolate A0 (9,67%), A1 (11%), A2 (10,67%), A3 (12%), A4 (13%), and A5 (6%).
 Keywords: Yeast, phosphate nutrient, biological control, Fusarium sp.

Effects of Varying Doses of Cocos Nucifera Water on Body Weight and Blood Biochemistry of Broiler Chicks

Effect of 2 varying doses of Cocos nucifera water (coconut water) was investigated on body weight and blood biochemistry of broiler chicks. The body weight, dressed weight and organ weights were determined by the use of standard weighing balance. The coconut water was aseptically collected from each coconut by piercing through the eye with sharp rod. The sterile coconut water was transferred into labeled sample bottle, corked and kept for the experiment. A total of 60 two weeks old anak 2000 broiler chicks were assigned into three treatments of 20 chicks each and replicated four times of 5 chicks per replicate in a completely randomized design. Treatment one (control) received 0.00mgcoconut water/kg body weight of birds. Treatment two and three received 200mg and 400mg of coconut water per kg bodyweight of birds respectively through oral intubation. The administration of experimental material was terminated after 28 days following an overnight fast. Blood was collected by venu–puncture via external jugular vein and discarded into two sample sets of bottles (one without anticoagulant and another with anticoagulant). The result obtained revealed a non-significant (P>0.05) non-dose dependent changes in live weight, dressed weight of broiler chicks with a significant (P<0.05) but non-dose dependent dressing percentage. The organ weight characteristics showed a non-significant (P>0.05), non-dose dependent weight changes in all the organs weighed except the gizzard that produced a significant (P<0.05), dose dependent reduction in weight as dosage of administration of coconut water increased. For haematological indicesthe result showed dose dependent significant (P<0.05) decrease in mean corpuscular haemoglobin concentration and relative white blood cell count but a significant (P<0.05) non-dose dependent decrease in haemoglobin concentration. Anon-significant, non-dose dependent changes were observed for red blood cells count and parked cell volume for doses of coconut water tested. For blood lipids, significant dose dependent decreases in total cholesterol and triacylglycerol were observed but a significant and non-dose dependent decrease in very low-density lipoprotein cholesterol. The serum low density lipoprotein (LDL) and high-density lipoprotein cholesterol revealed non-significant, non-dose dependent changes with the dose of coconut water administered. Alanine aminotransferase showed a significant increase but non-dose dependent activities for the two doses of administration under investigation whereas aspartate aminotransferase exhibited non-significant but dose dependent changes in activity. However, alkaline phosphatase revealed a dose dependent significant reduction in activity for the doses tested. Having no detrimental effects on body weight and organ weights with a significant (P<0.05) reduction in blood lipids, one will commend the use of coconut water in broiler production not above 400mg/kg body weight of birds due to its negative response to white blood cell count, haemoglobin concentration (though not correlated at all to RBC) and alanine aminotransferase activity in this study.

Nata De Coco Training for Villagers of Wangon Banyumas District

IbM entitled "Training Nata De Coco for Communities in Rural Wangon, Banyumas" is aimed to assist partners in solving the problems concerning the use of the coconut water and a lack of knowledge and skills of the communities in Wangon in the use of appropriate technologies for coconut water processing into food products named as Nata De Coco nutritious and high economic value product. This community engagement is purposed to: 1). encourages partners to utilize coconut water which has a lower sale value, 2). give briefing, training and skills for partners in the utilization of appropriate technology for coconut water processing into food products that are nutritious Nata De Coco and high economic value. IbM activity is done by providing training that includes some activities: 1) exposure of the material, 2) giving an example of making Nata De Coco, 3). practice of making Nata directly by the participants. Partners who attended the event IbM is household villagers of Wangon, Banyumas. Attendees were women who belonged to the productive age, but they are simply a housewife. From the implementation of IbMactivity, it can be concluded that the participants’ awareness and knowledge about the use of coconut water, and procedure of knowledge of making Nata De Coco are increased

Antioxidant and Cholesterol Esterase Inhibitory Properties of Supplementation with Coconut Water in Submerged Cultivation of the Medicinal Chinese Caterpillar Mushroom, Ophiocordyceps sinensis CS1197 (Ascomycetes).

We investigated the potential use of coconut water to supplement potato dextrose broth (PDB) in the production of Ophiocordyceps sinensis CS1197 by submerged cultivation. The basal PDB medium was modified by supplementation with tender coconut water (TCW) and mature coconut water (MCW) at 10% and 5% (v/v), respectively; these mixtures were cultured at 28°C for 14 days, with a pH of 7 and an inoculum volume of 10%. The addition of optimized levels of TCW and MCW improved the biomass yield by 2.2- and 2.5-fold, respectively, and adenosine, cordycepin, and polysaccharide content by 58% and 69%, 50% and 55%, and 19% and 27%, respectively. Antioxidant and cholesterol esterase (CE) inhibitory activities of the aqueous extract from O. sinensis CS1197 mycelia supplemented with TCW and MCW were high compared with those of the control, indicating that coconut water has a positive correlation with the enhanced antioxidant and CE inhibitory activities. These antioxidant and CE inhibitory responses were dependent on concentration, and the larger amounts of bioactives in O. sinensis CS1197 are beneficial in pharmaceutical formulations.

Use of coconut water and fertilizer for in vitro proliferation and plantlet production of Dendrobium ‘Gradita 31’

To improve the micropropagation of Dendrobium ‘Gradita 31’, coconut water (CW) and fertilizer media were used to enhance growth, proliferation, and germination of protocorm-like bodies (PLBs). PLBs formed when shoots approximately 0.4 cm long were cultured on semi-solid half-strength Murashige and Skoog (MS) basal medium containing 1 mg L−1 thidiazuron (TDZ) and 0.5 mg L−1 N6-benzyladenine (BA). After four rounds of 15-d subcultures, PLBs were initially proliferated in liquid half-strength MS medium supplemented with 0.3 mg L−1 TDZ and 0.1 mg L−1 α-naphthalene acetic acid (NAA) and subcultured monthly into fresh medium. When 15% (v/v) CW was added to half-strength MS medium, optimal growth and proliferation of PLBs resulted with the following principal findings: a maximum of 2.86 g total fresh weight (FW) of PLBs formed from 1 g of PLBs, an average of 0.46 g FW of PLBs added per subculture period, a total of 157.1 PLBs formed, an average of 25.3 PLBs added per subculture period, and a low percentage of PLB browning (20.7%). Half-strength MS medium containing 15% (v/v) CW with Rosasol® medium (liquid fertilizer; 1.5 g L−1 18 N:18P:18 K + 1.5 g L−1 25 N:10P:10 K + TE) resulted in a similar organogenic outcome with 25.5% PLB browning. PLBs germinated easily and rooted on Rosasol-supplemented plant growth regulator-free medium. In total, 180 Dendrobium ‘Gradita 31’ plantlets from all treatments were successfully acclimatized with 100% survival on a Cycas rumphii bulk substrate and grew well after repotting in a mixture of wood charcoal and C. rumphii bulk (1:1, v/v).

PENGARUH PENGGUNAAN AIR KELAPA (Cocos nucifera) TERHADAP PERTUMBUHAN TANAMAN SAWI (Brassica juncea L.)

Background: The research purpose is to know the influences use of coconut water on the growth of mustard plants. The materials used on this research are: Green Mustard Seed, Coconut Water, Water and Soil. Research carried out for 2 months, from 15 September 2010 till 15 November 2010.
 Method: The research using Design Randomly Complette, that consist of 5 treatments with each treatments: A0 = control without coconut water, A1 = volume of coconut water is 100 ml, A2 = volume of coconut water is 150 ml, A3 = volume of coconut water is 200 ml, and A4 = volume of coconut water is 250 ml. Each treatments repeated 3 times. Data were analyzed using Analysis of Variance and followed by the Smallest Real Test at level α 0,5% and α 0,1%.
 Result: The result of the researchshowed that, the use of coconut water give a real impact on the growth of mustard plants on it’s height and number of leaves, that obtained on treatments A4 = volume of coconut water is 250 ml.
 Conclusion: The volume of coconut water that most influence on the growth of mustard (Brassica juncea L.), on plant height and number of leaves, contained in a volume of 250 ml, followed by volume of 200 ml, 150 ml and a further volume of 100 ml, and Control (A0).

The Role of Coconut Water on Nicotine-Induced Reproductive Dysfunction in Experimental Male Rat Model

The coconut water is the most nutritious wholesome beverage in all coconut producing countries. Folk medicine describes the use of coconut water to reduce toxic effects of nicotine and alcohol in reproductive function in men, but its direct evidence is lacking. The aim of this study was to investigate the effects of tender and mature coconut water consumption on nicotine induced changes in sperm quality, spermatogenic cell density, activity of testicular marker enzymes for spermatogenesis and testosterone level of male healthy rats. Male Sprague Dawley rats were treated with tender and mature coconut water intragastrically and nicotine subcutaneously for 15 days. The findings indicated that the coconut water supplementation improves epididymal spermatogenic cell density (p ≤ 0.05), sperm motility and morphology which were altered by nicotine. The activity of acid phosphatase and alkaline phosphatase was increased (p ≤ 0.05), which indicates that treatment with coconut water provides significant protection of germinal epithelium from nicotine toxicity. Coconut water also shows a significant increase (p ≤ 0.05) in testosterone levels in nicotine treated rats. Coconut water ameliorates the reproductive toxicity caused by nicotine due to presence on nutrients L-arginine, ascorbic acid, minerals like calcium and magnesium.

Therapeutic use of coconut water

This paper is a review about therapeutic use of coconut water. We began with its chemical composition, pointing that it is reach in antioxidants, potassium, calcium, carbohydrates, and poor in sodium. The antioxidant activity from coconut water is of interest as a potential natural food antioxidant additive, a nutraceutical. When coconut water is used in hypertensive people, this brought about a large decrease in blood pressure, including increased frequency in urination suggest a possible diuretic action. Additionally, in all cases of persons consuming coconut water on a regular basis an increase in the plasma level of potassium is observed. This would be a contributing factor in the decreases in blood pressure, suggesting a potassium sparing natriuretic. Some time trial tests as the measure of exercise performance have determined the efficacy of coconut water and other beverages on enhancing hydration and performance following dehydrating exercise. In conclusion, coconut water can be used in cases of hypertension, rehydration in sports, together with early refeeding, as a home glucose electrolyte oral rehydration solution in the early stages of mild diarrhoeal disease, despite not having a balanced electrolyte composition. However, it should not be used in patients with severe diarrhoea, or in patients who are severely dehydrated and/or in whom renal function is impaired. Further studies should be conducted to better understand the effects of coconut water as a therapeutic agent.

Provision of low cost media options for in vitro culture of Celosia sp.

The composition of culture medium used for shoot regeneration has a great influence on cost of materials making of media. This study was conducted to investigate the effect of using four kinds of commercial starch or flour as alternative gelling agents and coconut water as an organic additive in the culture medium on the number of shoots produced for eight weeks in culture. Explants from stem segments of Celosia sp were cultured on medium containing either half strength of Murashige and Skoog (MS) or without MS combined with 100 g/l of each gelling agents and 70 ml/l coconut water. The findings showed that the use of young coconut water and alternative gelling agents have response to in vitro shoots regeneration of stem segments of Celosia sp. Higher revenue (12.33 ± 7.27) of shoots derived from stem segments results from the cultured media containing100 g/l of potato starch, 70 ml/l coconut water, household sugar and half strength concentration of MS (½ MS). Statistical analysis found no significant difference (P> 0.05) between culture media with different concentrations of MS with the number of shoots produced. The substitution of alternative gelling agents, use of coconut water and quantities of MS powder can reduce costs to medium preparation.

Coconut Water (Cocos nucifera) as Storage Media for the Avulsed Tooth

Avulsion is a condition of tooth displacement outside the socket due to the trauma. In a case of tooth avulsion case of per-manent teeth, the treatment of choice is replanting the tooth back to the socket. The main concern prior to tooth replanta-tion is to maintain the vitality of periodontal ligament (PDL) cells. The vitality of PDL cells is crucial to form new perio-dontal ligament tissue to support the teeth in order to achieve the success of replantation treatment. Therefore the tooth storage media is needed to maintain the vitality of PDL cells. The aim of the present literature review was to discuss the use of coconut water as an alternative storage medium of the avulsed teeth. Coconut water is biologically pure and steril, rich in amino acid, proteins, vitamins and minerals. The electronic composition of coconut water resembles the intracellu-lar fluid. The ability of coconut water to maintain the vitality of PDL cells is due its nutrient component such as protein, amino acid, vitamin and minerals. Previous study showed the ability of coconut water to maintain the viability of PDL cells was comparable to Hank’s balanced salt solution (HBSS), storage media that is recommended by the American As-sociation of Endodontics. In conclusion, coconut water is an effective storage medium for avulsed teeth to maintain the vitality of PDL cells. The advantages of the use of coconut water are economical and easily obtained, and therefore ideal to be recommended as a storage medium for avulsed teeth. DOI: 10.14693/jdi.v17i3.39

Enhanced regeneration in explants of tomato (Lycopersicon esculentum L.) with the treatment of coconut water.

A standardized protocol was developed to enhance the in vitro regeneration efficiency of five varieties of tomato from hypocotyls and leaf disc with the involvement of coconut water. Different concentrations of IAA and kinetin were used alone and in combination with 12% of coconut water. Significant differences for regeneration, time taken to regenerate and number of leaf primordial were observed for different treatments, type of explant, use of coconut water and also among varieties. Higher regeneration was obtained in Avinash followed by Roma and Rio Grande. Maximum regeneration 95.75% was obtained with 0.5 mgL -1 of IAA, 1.5 mgL -1 Kinetin along with 12% coconut water in Avinash and hypocotyl was proved to be better for regeneration than leaf discs. The inclusion of coconut water in the media significantly reduced the number of days taken for callus induction leading to regeneration, as compared to media without coconut water. Significant increase in number of shoot primordial was observed in hypocotyls as well as in leaf disc derived calli with the addition of coconut water. Plants obtained were transferred to the glass house in small pots of compost and higher frequency of survival was observed from plantlets obtained with the addition of coconut water.

Production of exopolysaccharides by Agrobacterium sp. CFR-24 using coconut water - a byproduct of food industry

The work is intended to explore the suitability of underutilized coconut water (a byproduct of food industry) for the production of exopolysaccharides (EPS) by Agrobacterium sp. CFR 24. Besides checking the suitability of coconut water for the production of water-soluble (WS) and water-insoluble (WIS) EPS, certain fermentation parameters, such as initial pH, incubation period and kinetics of EPS production were investigated. The coconut water medium was found to support the production of both types of EPS. The optimal initial pH and temperature was found to be 6.0 and 30 degrees C, respectively. In shake flask (150 rev min(-1)) studies, high-cell density inoculum resulted in the production of 11.50 g l(-1) of WIS-EPS and 4.01 g l(-1) WS-EPS after 72 and 96 h of fermentation, respectively. Coconut water was found suitable for the production of microbial EPS by Agrobacterium sp. CFR 24 strain. Under optimum conditions, it produced a good amount of WIS-EPS, which is comparable with that of the sucrose medium (11 g l(-1)). This is the first report on the use of coconut water as a fermentation medium for the production of any microbial EPS. Besides producing value-added products, use of this food industry byproduct, which is often being drained out, can significantly reduce the problem of environmental pollution.

The control of hypertension by use of coconut water and mauby: two tropical food drinks

In this study, the authors investigated the effect of regular consumption of two tropical food drinks, coconut (Cocos nucifera) water and mauby (Colubrina arborescens), on the control of hypertension. Twenty-eight hypertensive subjects were assigned to four equal groups and their systolic and diastolic blood pressures recorded for two weeks before and then for another two weeks while receiving one of four interventions. One group (the control) received bottled drinking water, the second group received coconut water, the third received mauby and the fourth group, a mixture of coconut water and mauby. Significant decreases in the mean systolic blood pressure were observed for 71%, 40% and 43% respectively of the groups receiving the coconut water, mauby and the mixture (p < or = 0.05). For these groups, the respective proportions showing significant decreases in the mean diastolic pressure were 29%, 40% and 57%. For the group receiving the mixture, the largest decreases in mean systolic and mean diastolic pressure were 24 mmHg and 15 mmHg respectively; these were approximately double the largest values seen with the single interventions.

The intravenous use of coconut water

Medical resources routinely used for intravenous hydration and resuscitation of critically ill patients may be limited in remote regions of the world. When faced with these shortages, physicians have had to improvise with the available resources, or simply do without. We report the successful use of coconut water as a short-term intravenous hydration fluid for a Solomon Island patient, a laboratory analysis of the local coconuts, and a review of previously documented intravenous coconut use.

Negative findings for use of coconut water as an oral rehydration solution in childhood diarrhea.

This study reports the chemical composition of coconut water during its maturation. Composition was measured at 5, 6, 7, 8, 9, 10, 11 and 12 months maturation. Concentrations of sodium and glucose, and the osmolality values showed great variation throughout maturation. The concentration of sodium remained constant between the 5th and 7th months (mean 2.9 mEq/l), but increased after the 8th month (mean 12.5 mEq/l). The concentration of glucose remained constant between the 5th and 8th months (mean 3392.4 mg%), but abruptly decreased after the 9th month (mean 820 mg%). Osmolality followed the variation of the glucose concentration averaging 377.3 mOsm/l up to the 8th month, then decreasing to 310.3 mOsm/l after the 9th month. This study showed great variability in coconut water composition during maturation of the fruit. In no instance did the coconut water contain sodium and glucose concentrations of potential value as an oral rehydration solution.

COCONUT WATER

The study on which we are reporting, fostered in the Service of the Chair of Pediatrics, Mercedes Hospital, Havana University, included the following aspects: Biologic value of coconut water, determined through study of its proteins. Importance of the amino acids found in the proteins of coconut water. Relation between the proteins in the endosperm of the coconut and those in the water. Experimental anaphylaxis in the rabbit as a means of determining the presence or absence of sensitizing (complex molecule) proteins in coconut water. Historicobibliographic survey of the medical use of coconut water. Ways of using coconut water in clinical pediatrics, with special reference to feeding of infants. Possibility of parenteral injection of coconut water and the scientific basis for it. <h3>BIOLOGIC VALUE OF COCONUT WATER</h3> In undertaking the study we were motivated by a desire to determine the scientific reasons for the use of coconut water in medical practice, since some of the reasons