Use Of Biofungicides Research Articles

Effectiveness of acid-tolerant antagonist-based biofungicide in the control of basal stem rot disease caused by ganoderma in oil palm seedlings on peat soil

Oil palm is an important plantation commodity in Southeast Asia. As the world’s largest palm oil producer, Indonesia has always been associated with global warming because most of its plantation areas are on peatlands and their management is considered not environmentally friendly. In addition to land fertility problems, a serious problem in peatland oil palm cultivation is basal stem rot disease caused by the Ganoderma fungus. Until now, there has been no effective method to control this disease. Biological control is an environmentally friendly alternative method and is the focus of current development, but for peatland conditions, its development is limited by the ultra-low peat pH factor. This study aims to determine the effectiveness of biofungicides based on acid-tolerant antagonists in controlling basal stem rot disease in oil palm seedlings on peat soil. The study was conducted by testing a bio-fungicide formulation in powder formulation containing 3 isolates of acid-tolerant antagonists, namely Trichoderma viride B3J19, Trichoderma harzianum E4J8, and Trichoderma harzianum 13EJ15. Testing was carried out on oil palm seedlings in greenhouses and fields. The results in the greenhouse showed that after six months of observation, bio-fungicides were able to reduce the intensity of the disease by 13.34% with 1 application, while 3 applications were 66.67% compared to the positive control. Meanwhile, in the field, the use of bio-fungicides with 1 application could reduce the intensity of disease by 27.29%, while 3 applications were 90.90%. These results indicate that bio fungicides in powder formula containing acid-tolerant antagonists have the potential to be used as biological control agents for oil palm basal stem rot disease caused by Ganoderma in peatlands.

The main diseases of spring wheat and the use of biofungicides in the northern Forest-Steppe of Ukraine

The purpose of the research was to identify the most common diseases of spring wheat grown in the Northern Forest-Steppe and to identify the most effective biological products used in the experiment against diseases of spring wheat. Methods. The experimental studies were conducted during 2021–2024 in the Northern Forest-Steppe in the field experiments of the Plant Protection Department of the National Research Center “Institute of Agriculture NAAS” in Chabany village, Fastiv district, Kyiv region. Object of research: spring wheat variety Rannіa 93. Weather conditions during the years of research contributed to the development of diseases. Results. As a result of the crops’ inspections, the following diseases were detected: powdery mildew (Blumeria grumines (DC.) Speer. (BLUMGR), leaf septoria (Septoria trittici Roberge ex Desm. (SEPTTR), ear blight (Sertoria nodorum (Berk.) Berk (SEPTNO), pyrenophora tritici-repentis (Died.) Drechsler and root rot (Fusarium (FUSASP), Bipolaris sorokiniana (Sacc.) Shoemaker (BIPOSO), Gaeumannomyces graminis (Sacc.) Arx & L.L.Olivier (GAEUGR), Pseudocercosporella herpotrichoides (Fron) Deighton (PSEUHE), Alternaria spp. ) (ALT), Fusarium spp. (FUSASP), brown rust Puccinia recondita f. sp. tritici Rob. ex Desm (P. triticina Erikss). Conclusions. The growing seasons of 2021–2024 were favorable for the development of wheat diseases, but the effectiveness of biological products during the spring wheat growing season was low and at the end of the growing season was less than 30%. The surveys conducted after three treatments showed that there is no significant effectiveness in relation to the control in variants with the use of different biological products based on different active ingredients. When applying biological products during the growing season, spring wheat yielded 0.1-0.9 t/ha more than in the control. The highest yield increase was in the variant with the use of a mixture of Sporazin 2 l/ha + Phytophyt 0.05 l/ha + Biostimix 0.5 l/ha + Xampan 0.25 l/ha, Sporomax T 1- 2 kg/ha + Sporomax B 2 kg/ha + Xampan 0.25 l/ha. Thus, the preserved yield indicates that the treatment of spring wheat crops with biological products has a positive effect on plants. Therefore, the use of biofungicides in the protection of spring wheat requires further research, and the study of the protection problem remains relevant.

Biofungicides Based on Plant Extracts: On the Road to Organic Farming.

Phytopathogenic fungi are responsible for diseases in commercially important crops and cause major supply problems in the global food chain. Plants were able to protect themselves from disease before humans played an active role in protecting plants. They are known to synthesize a variety of secondary metabolites (SMs), such as terpenes, alkaloids, and phenolic compounds, which can be extracted using conventional and unconventional techniques to formulate biofungicides; plant extracts have antifungal activity and various mechanisms of action against these organisms. In addition, they are considered non-phytotoxic and potentially effective in disease control. They are a sustainable and economically viable alternative for use in agriculture, which is why biofungicides are increasingly recognized as an attractive option to solve the problems caused by synthetic fungicides. Currently, organic farming continues to grow, highlighting the importance of developing environmentally friendly alternatives for crop production. This review provides a compilation of the literature on biosynthesis, mechanisms of action of secondary metabolites against phytopathogens, extraction techniques and formulation of biofungicides, biological activity of plant extracts on phytopathogenic fungi, regulation, advantages, disadvantages and an overview of the current use of biofungicides in agriculture.

Cercospora leaf blight and purple seed stain of soybean: A permanent challenge

AbstractCercospora leaf blight (CLB) and purple seed stain (PSS) are major diseases of soybean responsible for significant yield loss. These diseases are caused by Cercospora species, such as C. kikuchii and C. cf. flagellaris, among the main species. These pathogens overwinter in infested debris and infected seed. Due to the combination of no‐till farming, monoculture in large soybean cultivation areas and climate changes, these diseases have become serious and prevalent. At present, in addition to the practice of monoculture soybean cultivation, there is a lack of soybean varieties with genetic resistance to these diseases. As a result, the application of fungicides becomes an essential tool for effectively managing this pathosystem. However, in the main soybean‐producing countries, resistance to quinone outside inhibitors and benzimidazole fungicides has already been studied and documented. Furthermore, Cercospora spp. are believed to be naturally insensitive to succinate dehydrogenase inhibitors. Consequently, there is a growing interest in innovative and integrated tools for managing crop diseases. These new approaches include the use of biofungicides, biostimulants and plant defence inducers. Research testing new management tools in a complementary and integrated approach involves a short‐term challenge to improve CLB/PSS management in the field. This review provides comprehensive details on the taxonomy, identification and genetic diversity of the pathogen, its disease symptoms and host range. Additionally, it outlines epidemiological aspects, fungicide resistance and the latest advancements in tools and technologies, aiming to guide future research and strategies to address the escalating threat to soybean crops globally.

Monitoring root rot in flat-leaf parsley via machine vision by unsupervised multivariate analysis of morphometric and spectral parameters

Use of vertical farms is increasing rapidly as it enables year-round crop production, made possible by fully controlled growing environments situated within supply chains. However, intensive planting and high relative humidity make such systems ideal for the proliferation of fungal pathogens. Thus, despite the use of bio-fungicides and enhanced biosecurity measures, contamination of crops does happen, leading to extensive crop loss, necessitating the use of high-throughput monitoring for early detection of infected plants. In the present study, progression of foliar symptoms caused by Pythium irregulare-induced root rot was monitored for flat-leaf parsley grown in an experimental hydroponic vertical farming setup. Structural and spectral changes in plant canopy were recorded non-invasively at regular intervals using a 3D multispectral scanner. Five morphometric and nine spectral features were selected, and different combinations of these features were subjected to multivariate data analysis via principal component analysis to identify temporal trends for early segregation of healthy and infected samples. Combining morphometric and spectral features enabled a clear distinction between healthy and diseased plants at 4–7 days post inoculation (DPI), whereas use of only morphometric or spectral features allowed this at 7–9 DPI. Minimal datasets combining the six most effective features also resulted in effective grouping of healthy and diseased plants at 4–7 DPI. This suggests that selectively combining morphometric and spectral features can enable accurate early identification of infected plants, thus creating the scope for improving high-throughput crop monitoring in vertical farms.

Studierea creşterii şi productivităţii pomilor de nuc cultivaţi în sistem ecologic

The article presents the results of the researches carried out in 2020–2022 regarding the action of traditional and ecological agrotechnical processes on the growth and productivity of the Chandler variety walnut. Scientific data are presented regarding the trunk diameter, the tree height, the tree crown width, the average and total length of the annual growths, the content of nutrients in the tree leaves, the influence of fertilizers on the leaf surface and the mass of the leaves, the biological efficiency of the measures system for the use of biofungicides and bioinsecticides, as well as the fruit harvest in the 5 th - 7 th years of tree vegetation.

Management of Alternaria blight disease of mustard through biofungicides

Alternaria blight, caused by Alternaria brassicae, is a major disease affecting mustard crops, leading to significant yield losses. Traditional chemical fungicides have been extensively used to manage this disease, but concerns over environmental pollution, development of resistance, and potential health hazards have shifted the focus towards alternative strategies. Biofungicides, derived from natural sources such as microbes, plants, and their products, have emerged as promising alternatives for managing Alternaria blight while ensuring sustainability and minimal environmental impact. The purpose of the present study was the use of biofungicides as a more sustainable alternative for managing Alternaria blight in mustard at Amity University, located in Noida, Uttar Pradesh. The organic treatment included FYM, Neem oil, Trichoderma viride in different combinations. Pusa Sag1 variety was used for the field study. The present findings revealed the significance differences were recorded in different growth stages of mustard among the various treatment tested. It has been found that minimum disease incidence was observed in T5-Trichoderma viride + FYM + Neem oil (3%), followed by T4Trichoderma viride + Neem oil (13%), T3Trichoderma viride (15%), T2Neem oil(28%), T1FYM(28%). Biofungicides holds great promise for sustainable disease control while minimizing environmental risks and promoting agricultural resilience.

Assistance for generative nurseries and the use of Biofungicides (Trichoderma spp.) for coffee farmers in the Mokwam District, Kampung Kwau Manokwari

Coffee plant (Coffea arabica) is a superior plant to be developed in the Arfak mountain region due to suitable agro-climatic conditions. The way to develop it can be done in various ways, one of which is to develop it independently using seeds from farmers' gardens. This goal can be achieved if the local farming community is able to prepare seeds that have high growth potential and are healthy, free from pests and diseases, especially diseases that are transmitted through the soil (seed born pathogens). The method used includes first, mentoring and discussion at the Kwau Village Hall which includes lectures and discussions about generative nurseries and the use of Trichoderma spp to treat soil borne diseases. The second method is a demonstration of how to plant cocoa seedlings in the farmer's garden accompanied by the application of Trichoderma spp. Both methods were successfully implemented and the community showed high enthusiasm as seen from the questions asked. In this mentoring activity, 60 coffee seedlings were distributed to be developed by coffee farmers.

Purchasing Behaviour of Farmers for Bio-fungicide in Meerut District of Uttar Pradesh

In this study, an attempt was made to examine the variables affecting farmers’ satisfaction with bio fungicide use, the variables affecting their buying behaviour for the product and constraints faced by the farmers in usage of bio-fungicides. A total of 100 farmers were selected on the basis of snowball sampling. RBQ mean was calculated to find out the most influencing factors for purchasing of bio fungicide by the farmers. Garrett’s standard procedure was adopted to determine the significance of a number of different factors as expressed by respondents in the use of bio-fungicides. It was found that the retailers influence, quality, price of positives and the opinion leaders with implant agency were the important factors that affect the purchasing decision of farmers. Further, a substantial percentage of farmers in the research area were quite satisfied with the bio-fungicide’s efficacy, followed by its relative quality and history of widespread usage.

Biopreparations as an agro-ecological factor enhancement of biosafety in agrocenoses

The problem of environmentalization of agricultural production is extremely important in a scientific and applied sense, the main task of which is to provide the population with food products, prevent crop losses from harmful objects, and protect the environment from excessive chemical load. Accordingly, an alternative solution in overcoming the negative consequences of chemicalization of agricultural production and improving the quality of seed products is the use of environmentally safe plant protection measures. Protection against phytopathogenic microorganisms is an important component of technologies for growing various crops. After all, in soybean agrocenoses there is an accumulation of an infectious background of phytopathogenic micromycetes, among which species of the genus Penicillium, Aspergillus, Alternaria, and Fusarium predominate, which can cause outbreaks of many plant diseases (root rot, Alternaria, Fusarium, late blight, anthracnose, cercosporosis). Phytotoxic metabolites of necrotrophic phytopathogenic fungi, which are able to accumulate in soil, seeds and plant residues, deserve special attention. After all, mycotoxins lead to a decrease in the yield and quality of grain, as well as the quality of food products, which negatively affects their ecological safety, and this, in turn, can cause poisoning of humans and animals. The use of biofungicides is aimed at controlling diseases and reducing their harmful effects. Seed treatment leads to disinfection of seed material and protection of young plants from various infections. Therefore, the use of useful microorganisms, which are part of biological preparations, which effectively affect the processes of functioning of agroecosystems, is relevant.

Biofungicides for Improvement of Potato (Solanum tuberosum L) Production.

Potato (Solanum tuberosum L) cultivation originated from Peru in Latin America. The cultivation has spread fast across the globe due to its ability to cope in the warm tropical and temperate climate. It is spotted by the United Nations as the only tuberous crop that can compete with the cereals in productivity. Fungal disease infestation has been identified as a major challenge confronting the farmers during the cultivation and marketing processes. Farmers' reliance on Chemical fungicides has lost its credibility to the adoption of the use of biofungicides due to its toxic, high cost, and environmental hazard effects. The trend of the adoption of biofungicides by potato farmers is gaining ground at a fast rate. Various national governments are devising means of collaborating with the United Nations stakeholders through encouraging research funding and by organizing conferences that will enhance potato production. This could be achieved by minimizing losses through farmer's complete adoption of biofungicides. This review, therefore, examines the various botanicals with antimicrobial properties as potential biofungicide against fungi diseases of potato.

Bacillus spp. as Bio-factories for Antifungal Secondary Metabolites: Innovation Beyond Whole Organism Formulations.

Several fungi act as parasites for crops causing huge annual crop losses at both pre- and post-harvest stages. For years, chemical fungicides were the solution; however, their wide use has caused environmental contamination and human health problems. For this reason, the use of biofungicides has been in practice as a green solution against fungal phytopathogens. In the context of a more sustainable agriculture, microbial biofungicides have the largest share among the commercial biocontrol products that are available in the market. Precisely, the genus Bacillus has been largely studied for the management of plant pathogenic fungi because they offer a chemically diverse arsenal of antifungal secondary metabolites, which have spawned a heightened industrial engrossment of it as a biopesticide. In this sense, it is indispensable to know the wide arsenal that Bacillus genus has to apply these products for sustainable agriculture. Having this idea in our minds, in this review, secondary metabolites from Bacillus having antifungal activity are chemically and structurally described giving details of their action against several phytopathogens. Knowing the current status of Bacillus secreted antifungals is the base for the goal to apply these in agriculture and it is addressed in depth in the second part of this review.

ЭФФЕКТИВНОСТЬ БИОЛОГИЧЕСКИХ ФУНГИЦИДОВ ПРОТИВ ПЯТНИСТОСТЕЙ ЛИСТЬЕВ ОЗИМОЙ ПШЕНИЦЫ В УСЛОВИЯХ ЦЕНТРАЛЬНОЙ ЗОНЫ КРАСНОДАРСКОГО КРАЯ

The biologization of plant growing in modern conditions is becoming an urgent trend in agriculture, which helps to reduce the pesticide load. An important aspect of the transition to organic farming is the use of fungicides based on living microorganisms and their metabolic products to curb the development of diseases. Studies were carried out to assess the effectiveness of modern biofungicides against leaf septoria (Septoria tritici) and yellow spot (Pyrenophora tritici-repentis) of winter wheat - Vitaplan, SP, Gamair, SP, Pseudobacterin-2, Zh, Rizoplan, Zh, Trichocin, SP, Fitosporin -M, SP. Fungicide Amistar Extra, SK was used as a chemical standard. The work was carried out in the conditions of the central zone of the Krasnodar Territory in 2019–2020 on the variety Grom, susceptible to leaf spots. The meteorological conditions of the first year of research were favorable for the development of pathogens of leaf spots. In the second year, conditions were extreme, both for the growth of wheat plants and for the development of phytopathogens. For two years of research, the most effective biofungicides against leaf septoria were Fitosporin-M, SP, Pseudobacterin-2, Zh and Vitaplan, SP, the biological effectiveness was 50.0%, 50.0% and 47.1%, respectively; against yellow spot - Vitaplan, SP and Trichocin, SP, biological efficiency was 48.4% and 45.9%. The use of biological fungicides made it possible to save from 2.5% (Gamair, SP) to 12.6% (Trichocin, SP) of the winter wheat grain yield in comparison with the control (without treatment). The high economic efficiency of the use of biofungicides was established, the maximum net profit was noted in the variant treated with Fitosporin - M, SP (9540 rubles / ha)

Potential use of biofungicides and conventional fungicide for the management of Botrytis blossom blight in lowbush blueberries

ABSTRACT Botrytis blight is an economically important disease of lowbush blueberry that causes significant yield loss annually. In this study, the biofungicides, Diplomat 5SC; (polyoxin D), Timorex Gold; (tea tree oil), Fracture; (BLAD) and Serenade MAX; (Bacillus subtilis) were evaluated for their disease suppression potential against B. cinerea individually and in rotation with Switch® (cyprodinil and fludioxonil) under field conditions. Three applications of each biofungicide were made for the stand-alone treatments at 7–10 day intervals, with Switch® replacing the biofungicides during the second applications in the combined treatment. Results indicated that the products have the potential for use as a biofungicide in lowbush blueberries. All the stand-alone and rotational applications brought about a significant reduction in disease development, especially in 2019. The application of Diplomat 5SC; and Fracture®-Switch® rotation decreased disease development by over 63% in 2018. In 2019, all stand-alone treatments reduced disease development by more than 42%, whereas their rotation with Switch® reduced disease by over 69% at one site and at least 30% at another site. Stand-alone Diplomat 5SC® and Timorex Gold® along with Fracture®, Timorex Gold® and Serenade MAX; rotation resulted in over 20% more berries. This study suggests that the biofungicides and their integration with chemical fungicides have the potential as an alternative management strategy against Botrytis blossom blight to reduce the use of conventional fungicides and produce fruit with no detectable fungicide residues.

Assessment of the Effectiveness of Complex Use of Biofungicides on Spring Wheat

In 2018–2019, the studies were carried out in order to assess the effectiveness of the use of various biofungicides on spring bread wheat (Triticum aestivum L.) on grey wood soil in the Predkamsk zone of the Republic of Tatarstan. The agents based on Bacillus subtilis strain 63-Z (Baxis®) and Pseudomonas aureofaciens strain (Pseudobacterin 2®) were used as biofungicides. The chemical fungicides were used as a standard (for seed treatment – prothioconazole + tebuconazole (Lamador®); for spraying – spiroxamine + tebuconazole + triadimenol (Falcon®)). The research results showed that the use of biofungicides for seed treatment and spraying during the earing phase of wheat has a pronounced effect on the length of the stem and the number of grains in the ear. Under the influence of the use of biofungicides, the damage to plants by root rot, leaf septoria and powdery mildew was reduced. In terms of their effect on leaf septoria and powdery mildew, the chemical fungicides were somewhat superior to biological ones. The highest yield of spring soft wheat was obtained using the scheme in which the seeds were treated with Baxis and the plants in the earing phase were sprayed with Pseudobacterin 2. This scheme provided the increase in yield by 1.1 t / ha compared to control and by 0.29 t / ha when using standard chemical fungicides for seed treatment and spraying of crops. The use of such a scheme significantly reduces the cost of the protection of wheat plants from diseases. As a result of research, it was shown that with a weak and medium development of diseases, the use of biofungicides can be an effective alternative to the use of standard chemical fungicides.

Current status and prospects of the use of biofungicides against plant diseases

Plant pathogenic microorganisms cause great damage to the yield of agricultural crops and also reduce their commercial quality. This article highlights information on the level of damage caused to agricultural crops by pests, as well as the development of organic agriculture, which in recent years has received great attention in many developed countries. In addition, the data from literature were analyzed on the current state and problems of production of pesticides in the world and their use in agriculture, the use of biofungicides against plant diseases. The importance of synthetic pesticides, as well as, controlling the use of synthetic fungicides, and the use of alternative biofungicides in their replacement were also revealed. The article concludes on the need for public reforms and the role of systematic scientific research in creating a local biopesticides market.

Current status and prospects of the use of biofungicides against plant diseases

Current status and prospects of the use of biofungicides against plant diseases

Limitation of the distribution of the latent form of potato phomosis with the use of biofungicides

Goal. To establish the spread of the latent form of the phomosis pathogen in the western region of the Forest-Steppe zone and to study the possibility of crop protection through the use of biofungicides.
 Methods. In the process of work used laboratory (cultivation of pathogens) and field (analysis, processing of tuber material).
 Results. The results of research conducted during 2019—2020 to detect latent infection of phomosis rot of potatoes in the western part of the forest-steppe zone of Ukraine (Zakarpattia, Chernivtsi, Ivano-Frankivsk regions) are presented. Within two years, the highest damage to tubers by latent phomosis infection was observed in varieties Yavir and Vodogray in the Ukrainian Carpathians (Ivano-Frankivsk region, Verkhovyna district, Bystrets village; Zakarpattia region, Mizhhirya district, Maidan village; Zakarpattia region, Rakhiv district, Yasinya village; Chernivtsi region, Vyzhnytskyi district, Berehomet village), which reached 25—33 and 21.0—23.0%, respectively. In the plain zone of Chernivtsi region (Hertsaiv district, Bukivka village; Novoselytsia district, UkrNDSKR IZR) a significantly lower percentage of tuber lesions with a latent form of the disease was observed. The minimum rates of tuber damage, both latent form of phomosis and with existing external signs, were observed in the variety Slovyanka and were in the plain zone 4.5—5.5% and 7.0—8.0 in the mountain. When treating tubers with biofungicides, the least pathogen tuber damage was observed in the variants using Trichodermin-Bio, PhytodoDoctor and Planriz, where the number of infected tubers was 10.0, 11.0 and 12.5%, respectively. In the control variant, the percentage of affected tubers with latent form of phomosis was 2.1—3.2 times higher than in the experimental ones. The effectiveness of biofungicides was greatest when using Trichodermin-Bio and PhytoDoctor and was 68.3 and 65.1%, respectively. Less effective drug against the latent form of phomosis, compared with other options was Hetomic, where its technical efficiency was 52.4%.
 Conclusions. The causative agent of phomotic rot of potatoes (Phoma exigua var. Exigua) in latent form is a widespread pathogen in the western part of the Forest-Steppe zone. To limit the spread of latent infection of phomos, biological drugs Hetomik (0.4 kg/ha) were used, Gaupbsin (5 l/t), Planriz (2 l/t), Trichodermin-Bio (2 l/t), PhytoDoctor (0.3 l/t), which helped to reduce the damage to the tubers by a latent form of the disease by 2.1—3.2 times compared to the control.

Phytoncide action of essential oils of medicinal plants on the causative agents of broad beans alternariosis and cladosporiosis

Relevance. The use of chemical fungicides in protecting plants from pathogens is the most common way, despite the proven danger of pesticides. The use of biofungicides based on essential oils of medicinal plants, which also have phytoncidal properties, can be a suitable alternative to chemical preparations. In the years favorable for the development of alternariosis or cladosporiosis, spots appear on the plants, leading to necrosis and significant loss of seed productivity. Therefore, the search for new effective and safe means of combating fungal phytopathogens becomes relevant.Methods. Alternaria alternata (2018-1.1) and Cladosporium cladosporioides (2019-11.4) strains were isolated from leaves with signs of alternariosis and cladosporiosis, on which the essential oils Abies alba, Artemisia vulgaris, Caryophyllus aromaticus, Coriandrum sativum, Lavandula officinalis, Ocimum basilicum, Pimpinella anisum, Rosmarinus officinalis, Salvia sclarea, Thymus vulgaris. The antifungal activity of essential oils was evaluated by comparing the diameter of the experimental (with the addition of essential oil to the Petri dish) and control colonies using the author’s scale.Results. All studied essential oils had antifungal properties, but species-specificity was observed. Essential oils of basil, coriander and thyme showed a fungicidal effect on C. cladosporioides and A. alternata. Lavender oil proved to be a fungicide for A. alternata and a strong fungistatic for C. cladosporioides. To create biological products for plant protection against alternariosis, sage essential oil (moderate fungistatic effect) may also be of interest, and cladosporiosis - anise and rosemary oils.

Management of Botrytis blossom blight in wild blueberries by biological control agents under field conditions

Botrytis blossom blight is an important disease of wild blueberries in Canada that causes over 20% yield losses annually. Four trials (2 in each year) were set up in 2015 and 2016 to investigate the efficacy of biofungicides against Botrytis blossom blight in wild blueberry. Botector®, Fracture® and Serenade MAX® were evaluated alone and in rotation with Switch® and compared with a standard control program (Fontelis®, Switch®, and Pristine®). Three applications of each biofungicide were done for stand-alone treatment, and each rotated with Switch® as combined treatment. Fungicides were applied at 7-10-day intervals starting from pre-anthesis, white tip stage. Botector®, Fracture® and Serenade MAX® rotation with Switch® suppressed Botrytis blossom blight by over 65% and 60% less disease incidence and severity compared to the untreated control. Significant disease suppression was observed in stand-alone application of Botector®, Fracture® and Serenade MAX® in 2016 whereas they were not effective in 2015. The rotation of Fracture® and Serenade MAX® with Switch®, and stand-alone Serenade MAX® resulted in increased harvestable berry yield. The results suggest that the use of biofungicides in tandem with conventional fungicides in an integrated disease management program, can adequately suppress Botrytis blossom blights.