Use Of Agrochemicals Research Articles

The Problematic Issues of Unmanned Aerial Vehicle Application in Agriculture

The current rapid development of unmanned aviation is largely driven by the needs of the defense-industrial complex. In light of these events, the Russian industry has currently focused its attention on the development of unmanned aerial vehicles characterized by high payload capacity, autonomous flight range, and navigation precision. Considering the projected increase in the production rates of UAVs and the expansion of their functionalities, there is a pressing need for their active integration into various sectors of the economy, including agriculture. Despite the fact that the application of aerial agro-drones, like other aircraft, is regulated by the "Federal Rules for the Use of Airspace of the Russian Federation" (Government Resolution No. 138 dated March 11, 2010), there are currently no unified regulated approaches and requirements for the operation of unmanned aerial vehicles in agriculture. Given the growing relevance of issues related to the integration of unmanned aerial vehicles into agriculture, there is an increased need for the development of documents that regulate the use of agrochemicals for the treatment of agricultural land using these devices. The research objective if the development of a protocol for the inclusion of pesticides if the catalog approved for use through unmanned aerial vehicles.

Emerging Nanochitosan for Sustainable Agriculture

Chemical-intensive agriculture challenges environmental sustainability and biodiversity and must be changed. Minimizing the use of agrochemicals based on renewable resources can reduce or eliminate ecosystems and biodiversity threats. Nanochitosan as a sustainable alternative offers promising solutions for sustainable agricultural practices that work at multiple spatial and temporal scales throughout the plant growth cycle. This review focuses on the potential of nanochitosan in sustainable agricultural production and provides insights into the mechanisms of action and application options of nanochitosan throughout the plant growth cycle. We emphasize the role of nanochitosan in increasing crop yields, mitigating plant diseases, and reducing agrochemical accumulation. The paper discusses the sources of nanochitosan and its plant growth promotion, antimicrobial properties, and delivery capacity. Furthermore, we outline the challenges and prospects of research trends of nanochitosan in sustainable agricultural production practices and highlight the potential of nanochitosan as a sustainable alternative to traditional agrochemicals.

Reshaping the field: technocratic facts, high wheat yields, and the making of an ecological disaster

ABSTRACT Today the Yaqui Valley, a renowned farming region in northern Mexico, is a top emitter of greenhouse gas. Made famous as the birthplace of the Green Revolution, the high-yielding seeds that transformed the world’s farming were engineered in the research station located in the valley. Research led by agronomist Norman Borlaug not only transformed seeds but significantly altered the contemporary understanding of fertilizer use. Five times as much fertilizer as had previously been used became part of the needed recipe to produce high yields of wheat. Decades later, soil and groundwater contamination could be traced to the heavy use of agrochemicals. This article examines pre-1970s writings, both scientific publications and an oral history, to understand how this leap in fertilizer use became normalized and persisted for nearly seven decades.

Haematological parameters and biochemical indices of African catfish (Clarias gariepinus) exposed to glyphosate-based herbicide (Force up®) for 96 hours

IntroductionGeometric aquaculture growth has resulted in exponentially increasing use of agrochemicals as either parasiticides or herbicides in the aquaculture environment. This study determines some of the toxicological (haematological and biochemical) effects of glyphosate-based herbicides on non-target aquatic animals using Clarias gariepinus as the animal model.MethodSeventy-five apparently healthy adult C. gariepinus (300 g) were sourced from a local farmer and acclimatised for 2 weeks; of these, sixty subjects were divided into four treatment groups (fifteen fish per group and five replicates per unit) by simple randomisation and labelled as T0 (control), T1 (first treatment), T2 (second treatment), and T3 (third treatment). The treatments were replicated thrice. Four concentrations of Force up® [0 mL, 0.15 mL (0.003 mL/L or 5.1 mg/L), 0.225 mL (0.0045 mL/L or 7.65 mg/L), and 0.3 mL (0.006 mL/L or 10.2 mg/L) were added to a 50-L tank of water for T0, T1, T2, and T3, respectively. Approximately 5 mL of blood was collected from the fish in each treatment group 96 h post-exposure for measurement of the blood parameters and biochemical indices using standard analytical methods as well as calculation of the mean values.ResultThe mean values of the packed cell volume, haemoglobin concentration, red blood cell count, and white blood cell count compared to the control group showed an initial increase at T1 but decreased as the glyphosate concentrations increased at T2 (0.0045 mL/L) and T3 (0.006 mL/L). The platelet mean values decreased at T1, increased at T2, and decreased at T3, while the mean values of the corpuscular volume, corpuscular haemoglobin, and corpuscular haemoglobin concentration increased with glyphosate concentration, with the mean corpuscular haemoglobin concentration decreasing at T2. Only the platelet value was statistically significant at a p-value of <0.05 using ANOVA and post hoc Tukey test. The biochemical indices showed decreases in the mean values of aspartate transaminase, blood urea nitrogen, creatinine, and triglycerides at T1, increases at T2, and decreases at T3, while the total protein (g/dL), cholesterol, alanine transaminase, and alkaline phosphatase values showed increases at T1 and decreases at T2 and T3. All these values were not statistically significant based on ANOVA and had p-values >0.05.DiscussionBased on the results of this study, it is deduced that glyphosate-based herbicide (Force up®) has genotoxic, hepatotoxic, and nephrotoxic effects on C. gariepinus even at sublethal doses, with more adverse effects at increasing concentrations.

Understanding the Factors Influencing Generation X Consumers' Purchase Intention Towards Organic Vegetables in Chiang Mai: A Focus on Health Consciousness and Subjective Norms

Background and Aim: The escalating use of agrochemicals in vegetable cultivation has raised concerns about potential harm to human health and the environment, thus fueling a rising demand for organically grown vegetables. Notably, Generation X consumers, demonstrating a heightened awareness of the health and environmental consequences of their food choices, are at the forefront of this movement. This study investigates the impact of health consciousness and subjective norms on the purchase intention of organic vegetables among Generation X consumers in Chiang Mai province, Materials and Methods: This study investigates the influence of health consciousness and subjective norms on the purchase intention of organic vegetables among 400 Generation X consumers in Chiang Mai, Thailand. A quantitative cross-sectional survey was conducted using a structured questionnaire. Data analysis involved descriptive statistics, correlation analysis, and multiple regression analysis. Results: The findings reveal that both health consciousness and subjective norms significantly influence the purchase intention of organic vegetables. Subjective norms, particularly the perceived social pressure to consume organic produce, emerged as a slightly stronger predictor of purchase intention compared to health consciousness. Conclusion: This study underscores the importance of considering both health-related concerns and social influences in promoting organic vegetable consumption among Generation X consumers. Targeted marketing strategies that emphasize the health benefits of organic produce and leverage social reinforcement can effectively enhance purchase intention.

Impact of the Use of Agrochemicals and Organic Fertilizers in the Creating of Agrobiodiversity in the Aymara Communities of Puno

Impact of the Use of Agrochemicals and Organic Fertilizers in the Creating of Agrobiodiversity in the Aymara Communities of Puno

Soil arthropod diversity, richness, and abundance in agroecological and conventional cotton production systems in Chaco, Argentina

In Argentina, agroecology has grown in last years as a scientific paradigm that seeks to design and evaluate agroecosystems considering sustainability, complexity, and uncertainty. Diversity is a key factor in the design and management of production systems and a necessary component for conservation biological control and the reduction of agrochemicals use. Cotton (Gossypium hirsutum) crop in northern Argentina is usually managed with high load of agrochemicals: agroecological production arises as an alternative. This study evaluated the diversity, richness, and abundance of soil arthropods using pitfall traps in two experimental cotton plots under conventional (CONV) and agroecological management (AE) in Chaco, Argentina. AE system presented higher diversity and richness of predators compared with CONV, even when natural preparations were used for pest control. The phytophagous arthropods showed higher diversity and richness in CONV, even when pyrethroid insecticides were applied for pest control and preventively. The abundance in AE was lower for predators and higher for phytophagous arthropods. Agroecological production of cotton can be a tool that favors conservative biological control and an alternative for areas where protecting the health of farmers and the ecosystem is a priority.

Hydrophobic deep eutectic solvent-ferrofluid microextraction followed by liquid chromatography-mass spectrometry for the enantioselective determination of chiral agrochemicals in natural waters.

The increasing use of chiral agrochemicals sold as racemic formulations raises concern for the negative impacts that inactive enantiomers can have on aquatic life and human health. The present work just focuses on the determination of ten chiral pesticides in river water samples by applying a ferrofluid-based microextraction followed by their stereoselective liquid chromatography analysis. To develop the ferrofluid, magnetite nanoparticles were prepared and coated with oleic acid and then dispersed in a hydrophobic natural deep eutectic solvent (NaDES), composed ofL-menthol and thymol (1:1). The stable colloidal dispersion was characterised by scanning electron microscopy, thermogravimetric analysis, energy-dispersive X-ray spectroscopy and attenuated total reflection Fourier transform infrared spectroscopy. The analyte microextraction from 5ml river samples was performed using 50µl of ferrofluid, while acidified acetonitrile (150µl) was used to break down the ferrofluid and solubilise the NaDES containing the analytes. All the extracts were analysed by high-performance liquid chromatography-tandem mass spectrometry. For each analyte, the baseline separation of isomers was achieved on a Lux i-Amylose-3 column (amylose tris(3-chloro-5-methylphenylcarbamate) working in reversed-phase mode; the combination with mass spectrometry detection allows the overall separation of 24 isomers (ten chiral analytes among which eight containing a single (one) chiral centre, one with two chiral centres and the last one existing in four stereoisomeric forms, due to the presence of two regioisomers with a chiral carbon) within 37min. The method showed very good figures of merit in terms of recoveries (77.7-97.5%), intra-day and inter-day precision (2.7-7.7% and 6.9-14.9%, respectively), limit of detection (0.01-0.35µg/L), limit of quantitation (0.03-1.20µg/L), linear dynamic range, and intra-day and inter-day accuracy (1.2-14.8% and 1.8-15.0%, respectively). The presented method was able to detect 14 out of 24 isomers at the preventive limit established by the Italian legislation for single pesticide (that for a chiral pesticide is the sum of all its isomers) in surface waters, set at 0.1µg/l. Finally, the method was evaluated using AGREEprep and ComplexGAPI metrics, compared with other ferrofluid-based methods, and applied to the analysis of water samples from two Italian rivers (the Nera River and the Tiber River), providing to be sustainable and reliable for the application to real river matrices.

ЭФФЕКТИВНОСТЬ БИОПРЕПАРАТА БИОСТИМ НА ПОСЕВАХ КУКУРУЗЫ РОСС 140 СВ

The studies were conducted to study the effect of mineral fertilizers and the certified biopreparation Biostim Corn on the growth of the Ross 140 SV corn hybrid under irrigation. The field experiment was carried out in the conditions of the Republic of Tatarstan in 2021-2023. The object of the study was the Ross 140 SV hybrid zoned in the seventh region (Middle Volga region), bred by Krasnodar Research Institute of Agriculture named after P.P.Lukyanenko. In the experiment, various schemes for using the Biostim Corn preparation separately and in combination with Maksim Gold fungicide were studied. The use of Biostim Corn as a seed dressing before sowing at a dose of 2 l/t helps to increase the productivity of green mass. Its combined use with Maksim Gold seed dressing is even more effective. Against the background of the application of the calculated rates of mineral fertilizers, this ensures the formation of an additional 1.8 t/ha of green mass and an excess of the planned yield of 50 t/ha. Replacing Maksim Gold seed dressing with Biostim Corn increases the efficiency of using mineral fertilizers and foliar feeding. The profitability of green mass production of corn in the variant with the integrated use of agrochemicals is 73.3%, the conditional net income is 33.9 thousand rubles/ha, the cost of 1000 feed units is 4.6 thousand rubles. The use of the biopreparation Biostim Corn in the technology of cultivating the hybrid crop Ross 140 SV on irrigation allows you to improve the quality of seeds, increase the productivity of green mass and reduce the cost.

Comparison of plant microbiota in diseased and healthy rice reveals methylobacteria as health signatures with biocontrol capabilities.

Rice (Oryza sativa) is a staple food worldwide, but its production is under constant pressure from both abiotic and biotic stresses, resulting in high use of agrochemicals. The plant microbiome harbours microorganisms that can benefit plant health and provide alternatives to the use of agrochemicals. The composition of plant microbiomes depends on many factors (soil composition, age, and health) and is considered a primary driver of future plant health. To identify plant microbiomes that protect against disease, we hypothesised that asymptomatic rice plants in fields under high pathogen pressure (i.e., healthy islands of plants among predominantly diseased plants) might harbour a microbiota that protects them from disease. We sampled healthy and leaf-diseased plants in rice fields with high disease incidence in Cambodia and profiled their microbiota at leaf, root, and rhizosphere levels using 16S V3V4 and 18S V4 amplicon barcoding sequencing. Comparison of amplicon sequence variants (ASV) of the microbiota of healthy and diseased samples revealed both disease and healthy signatures (significant enrichment or depletion at ASV/species/genus level) in both fields. The genera Methylobacterium and Methylorubrum were identified health taxa signatures with several species significantly enriched in healthy leaf samples (Methylobacterium indicum, Methylobacterium komagatae, Methylobacterium aerolatum, and Methylorubrum rhodinum). A cultivation approach on rice samples led to the isolation of bacterial strains of these two genera, which were further tested as bioinoculants on rice leaves under controlled conditions, showing for some of them a significant reduction (up to 77%) in symptoms induced by Xanthomonas oryzae pv. oryzae infection. We validated the hypothesis that healthy plants in fields under high disease occurrence can host specific microbiota with biocontrol capacities. This strategy could help identify new microbes with biocontrol potential for sustainable rice production.

Causes and effects of contamination of water sources due to excessive use of agrochemicals and pesticides: bibliometric analysis

ABSTRACT This article conducts a bibliometric analysis of water pollution caused by agricultural pesticides, focusing on theoretical and methodological trends. The analysis uses Scopus databases and Bibliometrix and VOSviewer software tools to analyze 2,330 documents from 2013 to 2022. The research highlights the importance of environmental, agricultural, and biological sciences in addressing water pollution. The study suggests improving pesticide use, reducing fertilizer applications, and addressing the harmful impacts of contaminated fish on human health. The restriction of glyphosate use in the European Union caused economic losses for companies, and a follow-up on glyphosate use in crop applications was proposed. The study also evaluates chemical concentrations and recommends addressing aquatic ecotoxicological risks. Future research trends include increasing water use efficiency and quality, hydrogeological studies, watersheds and land use, and climate modeling and impacts. The research is unique in addressing both research trends and knowledge gaps in water pollution research in various aspects.

Smallholder RSPO certification, economic benefits and agrochemical use

Abstract Sustainability standards, such as the Roundtable for Sustainable Palm Oil (RSPO), can potentially mitigate the trade-offs between economic benefits and environmental harm of oil palm expansion. Using unique primary household and farm level panel data collected from Jambi Province, Indonesia, we first document the differences in agricultural inputs and outputs between certified and non-certified smallholder farmers. Second, we illustrate the potential implications and mechanisms of certification. In this study, therefore, we aim to first document agricultural input and output differences between certified and non-certified smallholder farmers, second to illustrate potential effects of certification with observational data and third to provide guidance for future rigorous causal analyses. We find generally positive correlations between RSPO certification, land productivity, profits, and fertilizer use, and a negative correlation with toxic herbicide use. However, we do not find price premiums for RSPO-certified farmers. Overall, the results of this study are indicative of potentially modest effects of RSPO-certification that may operate through higher productivity due to improved management practices that remain agrochemical intensive but avoid some particularly harmful chemicals. Our findings are relevant to better understand the strong sustainability claims of RSPO marketing and to provide guidance for rigorous causal experimental and quasi-experimental studies.

Agroecological farmer perceptions and opinions towards pest management and biodiversity in the Argentine Pampa region

ABSTRACT Agroecology has recently become an alternative production model that tries to face the negative environmental and socioeconomic consequences of the agricultural model based on excessive agrochemical use and no-tillage practices. It searches for alternatives to pest management so as to increase yields while promoting biodiversity conservation. Our goal was to know the perception and opinion of agroecological farmers of the Pampa region on crop pests, the techniques they use for pest management and their perceptions on biodiversity. A survey was conducted which provided 103 responses. The main pest mentioned was ants. Of farmers surveyed, 64.71% claim that their main pest significantly reduces their yields, 79.42% claim that they monitor their crops for pests, although those with over 25 years of experience tend not to. Farmers have a mostly positive perception of biodiversity, particularly birds even though many identify pigeons and parrots as damaging. These results make it possible to describe the agroecological Pampa farmers and to show some common pest issues that need to be observed to benefit production in the area. The study highlights the importance of biodiversity for these farmers and their willingness to use tools that attract birds that could potentially manage pests.

Determinants of net value added to produced and processed paddy rice in South East, Nigeria

The study principally examined the determinants of net value added to produced and processed paddy rice in South east Nigeria It specifically, identified the socio-economic characteristics of the respondents, analyzed the determinants of net value added to produced and processed paddy rice as well as ascertained the constraints to processed and produced paddy rice. Multi-stage sampling technique involving purposive and random sampling methods was used to select 240 respondents. Primary data were collected on rain-fed production season using structured questionnaires administered to the respondents by personal interview. Descriptive statistics,, multiple regression and principal component analysis were used to analyze the data. Results obtained showed that gender distribution of the respondents was 63%males and 38% females. More respondents are between the ages of 41-50 and31-40 respectively and house hold size of 4-6 .Farming experience showed that 136 respondents representing 57% had engaged in the business between 11-20yrs.The results also showed that 67% of the respondents accessed farm credit while 25% did not.66% of the respondents belonged to farmers association while 75% were not visited by extension agents. Variables (age, gender, years in school, household size, years of experience, training/extension visits, costs incurred, value addition technique and market price) significantly influenced the net value added while marital status, membership of cooperative, and access to credit did not. Constraints to paddy producers and processors were accessibility to farm machine, poor infrastructure and scarcity/high cost of labor, low access to credit/grant; low proactive measures to flood and drought control; insufficient knowledge of agro-chemicals, and late provision of essential training and services; and processors: use of outdated rice milling machines, high cost of modern rice processing machines, low access to modern rice processing machineries and low acceptability of local rice. Recommendations include; establishment of farm machinery hiring centers for producers and supply of modern rice milling machines to rice processing clusters, development of dams and irrigable lands, training and re-training on access to fund, utilization of biodegradable wastes from paddy rice, proactive measures of flood and drainage control, adequate use of agro-chemicals, use of modem techniques, and involvement of National Agency for Food and Drug Administration and Control in quality control. Keywords: Agriculture, Paddy Rice, Value Addition, Nigeria.

Anthropogenic nitrogen accumulation potential of Okinawa mangroves in Japan

The extent of the stable nitrogen (N) isotope ratio (δ15N) of mangrove leaves reflects the anthropogenic N accumulation potential of mangroves. Therefore, the present study was designed to investigate the N accumulation potential of Okinawa mangroves in Japan using three ecological indicators from four mangrove watersheds. The dissolved inorganic nitrogen (DIN) concentration in mangrove creeks, the leaf δ15N and the soil δ15N are considered immediate indicators, short-term indicators, and long-term indicators, respectively. The four mangrove watersheds are classified into two groups, human-affected and forested mangroves, based on the relative land use ratio (%) of the watersheds. The observed values of the ecological indicators were subsequently compared between two groups of watersheds to determine the relative ecosystem conditions. The results showed that both the leaf δ15N (0 to 9 ‰) and the soil δ15N (1.5 to 8.0 ‰) values are significantly greater in human-affected mangroves than in forested mangroves. The DIN of creek water samples does not indicate an immediate risk of excess N input from human perturbation in mangroves. However, the significant relationships among the indicators reflect the anthropogenic N accumulation potential of mangroves in Okinawa, Japan. These findings are highly important, especially for policymakers, environmentalists, and related stakeholders, for initiating conservation and management practices for mangroves. Controlled, limited, and/or restricted human perturbation; proper management of municipal wastes; and planned use of agrochemicals upon necessity may help reduce anthropogenic N inputs in mangrove ecosystems in Okinawa.

"A novel hybrid deep learning framework for enhanced segmentation of rice leaf diseases using attention-driven efficientnet models"

Rice diseases such as bacterial leaf blight, brown spot, and blast pose significant threats to global food security by reducing crop yields, making early and accurate detection crucial. This study aims to improve automated segmentation of rice leaf diseases using advanced deep learning techniques, specifically U-Net with MobileNetV2, DeepLabV3+ with EfficientNetB4, and U-Net with EfficientNetB7 integrated with attention gates. The models were evaluated on a dataset of diseased rice leaves for segmentation accuracy, computational efficiency, and robustness in real-world conditions. Results show that the U-Net with EfficientNetB7 and attention gates outperforms the other models, particularly for complex leaf images, achieving superior accuracy and generalization. This research provides a practical, real-time solution for early disease detection in rice, contributing to precision agriculture by helping reduce crop losses and optimize the use of agrochemicals, ultimately promoting sustainable crop management.

Green Consumption Values and Environmental Concerns Nexus: The Moderating Role of Buying Involvement in Organic Food Consumption in Pakistan

ABSTRACTThe food industry is a major contributor to climate change and has been linked to environmental and health issues due to excessive use of agrochemicals. To address these issues, responsible consumption and production have emerged. Organic certification is a common strategy for assuring consumers about sustainability. However, there is little research on organic food consumption in emerging countries. This study aims to examine the impact of green consumption values on environmental concerns using the theory of consumption values and also looks at the moderating effects of buying involvement. The researchers gathered data from 48 farmer markets in Punjab, Pakistan. They analyzed 730 respondents' data and found significant positive correlations between environmental concerns and multi‐dimensional green consumption values. The epistemic value was identified as a highly significant predictor of environmental concerns. The studied relationships are influenced by the buyer's level of involvement (low, average, and high), with significant differences in the connections between environmental concerns and consumption values among consumers with different levels of buying involvement. The findings have important implications for increasing organic food consumption in the mainstream market. This initiative is a significant milestone in achieving Responsible Consumption and Production (SDGs‐12th), as organic production benefits human health and promotes a sustainable environment.

Prevalence of bean scab caused by Elsinoë phaseoli and challenges associated with bean cultivation in Kenya.

This study investigated the prevalence of scab caused by Elsinoë phaseoli causing yield losses on beans in Kenya. The research focused on common practices and challenges faced by subsistence farmers with the aim of providing insights into scab prevalence, impact, and potential management challenges. A structured questionnaire was employed in a survey conducted in 2022 and 2023, covering major bean-growing regions using a three-stage sampling design. Data from 128 bean farmers included information on farm size, seed sources, cropping systems, awareness of challenges, and pest/disease management practices. Scab prevalence was determined by scouting for symptoms, with a total of 84 farms surveyed in 2021. The incidence of bean scab was confirmed in all surveyed clusters, indicating its widespread occurrence across various agro-ecological zones. Farmers exhibited common practices such as preference for uniform bean seeds (61%), use of uncertified seeds (83%), intercropping (80%), and limited crop rotation. Challenges included disease and pest infestations, with limited diversity in management options. Confirmation of the presence of bean scab in diverse agro-ecological zones emphasizes its importance and the need for further research on its impact and epidemiology. Challenges with crop rotation were evident due to small farm sizes and subsistence-focused farming. The study recommends further research for a comprehensive understanding of the link between increased scab importance and current bean farming practices such as short rotation periods and the use of susceptible varieties. Training programs are also vital to improve farmers' knowledge on safe agro-chemical use, ensuring sustainable constraint management in common bean cultivation in Kenya.

Protein Hydrolysates—Production, Effects on Plant Metabolism, and Use in Agriculture

Agriculture is facing challenges to produce more food in a climate scenario that works in the opposite direction. To amend this, agriculture has to invent new ways of making more with less. Interest in using by-products and finding new ways to utilize them has been increasing in recent years. The use of protein-rich sources for protein hydrolyzation and the use of these protein hydrolysates as biostimulants in plant production have been increasing. These mixtures are mainly produced by chemical and enzymatic hydrolysis from agro-industrial protein-rich by-products of animal, plant, and algal origins. The application of PHs has the potential to alleviate environmental stress; improve plant growth; and increase productivity, fruit yield, and abiotic-stress tolerance in agricultural crops. The use of these biostimulants offers a way to reduce the use of agrochemicals and agrees with the “do more with less” task in the future of agricultural production. This review gives an insight into the production of PHs, referring to sources of raw materials and methods of hydrolysis, the uptake and translocation of PHs, their effect on plant growth, the development and physiology, their role in alleviating stressful conditions, and their use in agriculture. The beneficial effects of PHs on different aspects of plant physiology, metabolism, and plant functioning under stressful conditions are evident. Inconveniently, crops, and sometimes even cultivars, are affected differently based on the way that PH is applied, the timing, and the concentration applied. Further research is needed to elucidate the mechanisms by which the components of PHs modify plant physiology and metabolism.

Profiling the biocontrol agents, nitrogen-fixing bacteria, and indole acetic acid-producing bacteria in anthill soil

Due to the adverse ecological impacts of prolonged and excessive use of agrochemicals, many researchers have called for the adoption of eco-friendly materials in farming. Consequently, the aim of this study is to profile the biocontrol agents, nitrogen-fixing, and indole-acetic-acid-producing bacteria present in anthill soil. Anthill soils and adjacent soils were collected, and their physicochemical properties were analysed using standard analytical methods. Viable bacteria were isolated and screened for plant growth-promoting (PGP) activity using standard biochemical, morphological, and bacteriological methods. The PGP capacity of the isolates was evaluated using standard protocol for nitrogen fixation and indole-acetic-acid production, while antagonistic effect against plant pathogens was evaluated using the disk diffusion assay. Results revealed that Pseudomonas aeruginosa, Klebsiella pneumoniae, and Bacillus sp. isolated from the anthill soil demonstrated PGP characteristics including IAA production and nitrogen fixation. Bacillus sp. exhibited zones of clearance with values of 20.00 ± 1.50 mm, 20.00 ± 1.75 mm, 17.00 ± 1.33 mm, and 17.00 ± 1.33 mm against Aspergillus niger, Trichoderma sp., Penicillium sp., and Fusarium sp., respectively. This study demonstrated that anthill soils contain beneficial microbes with the potential to stimulate plant growth and suppress soil-borne plant pathogens.