Use In Poultry Farming Research Articles

Antimicrobial resistance and genomic characterisation of Escherichia coli isolated from caged and non-caged retail table eggs in Western Australia

Foodborne exposure to antimicrobial-resistant bacteria is a growing global health concern. Escherichia coli (E. coli) is well recognised as an indicator of food contamination with faecal materials. In the present study, we investigated the occurrence of E. coli in table eggs sold at retail supermarkets in Western Australia (WA). A total of 2172 visually clean and intact retail eggs were purchased between October 2017 and June 2018. A single carton containing a dozen eggs was considered as a single sample resulting a total of 181 samples. The shells and contents of each sample were separately pooled and tested using standard culture-based methods. Overall, generic E. coli was detected in 36 (19.8%; 95% confidence interval: 14.3; 26.4) of the 181 tested retail egg samples. We characterised 100 of the recovered E. coli isolates for their phenotypic antimicrobial resistance using minimum inhibitory concentration (MIC). A subset of E. coli isolates (n = 14) were selected on the basis of their MIC patterns, and were further characterised using whole genome sequencing (WGS). Fifty-seven (57%) of the recovered generic E. coli isolates (n = 100) were resistant to at least one of the 14 antimicrobials included in the MIC testing panel, of which 22 isolates (22%) showed multi-class resistance. The highest frequencies of non-susceptibility of E. coli isolated from WA retailed eggs were against tetracycline (49%) and ampicillin (36%). WGS revealed that tet(A) and blaTEM-1B genes were present in most of the isolates exhibiting phenotypic resistance to tetracycline and ampicillin, respectively. The majority (98%) of the characterised E. coli isolates were susceptible to ciprofloxacin and azithromycin, and none were resistant to the cephalosporin antimicrobials included in the MIC panel. Two isolates demonstrated reduced susceptibility to ciprofloxacin, with MICs of 0.125 and 0.25 mg/L, and WGS revealed the presence of plasmid mediated qnrs1 gene in both isolates. This is the first report on detection of non-wild-type resistance to fluoroquinolones in supermarket eggs in Australia; one of the two isolates was from a cage-laid eggs sample while the other was from a barn-laid retail eggs sample. Fluoroquinolones have never been permitted for use in poultry farms in Australia. Thus, the detection of low-level ciprofloxacin-resistant E. coli in the absence of local antimicrobial selection pressure at the Australian layer farms warrants further research on the potential role of the environment or human-related factors in the transmission of antimicrobial resistance. The results of this study add to the local and global understanding of antimicrobial resistance spread in foods of animal origin.

Experimental Demonstration of the Structure of Silar Synthesised Iron Lead Sulphide (PbS) Thin Films for Poultry Farms in Agbaja Unuhu-Ebonyi State-Nigeria

The effect of iron-doping on lead sulphide (PbS) thin films deposited on glass substrates via successive ionic layer adsorption (SILAR) Technique using lead Nitrate, Pb(NO3)2, thiourea, Iron (II) Chloride dehydrate(Fe Cl2.2H2O), ethanol and ammonia by in alkaline medium annealed between 283K and 500K was investigated. The X-ray diffraction (XRD) Analysis and Scanning electron microscopy (SEM) were done to determine the structure and morphology respectively for use in poultry farms in Akpabuyo-Cross River State. The XRD showed PbS thin films being cubic and face-centred crystalline nanoparticles.

Evolution of the physical and physico-chemical parameters of compost resulting from a mixture of oasis waste and poultry manure

The purpose of this study is to monitor the evolution of some physical and physico-chemical parameters of four types of compost prepared from the mixture of oasis waste and poultry manure in different proportions. During the trial, which lasted 218 days, the temperature rises during the thermophilic phase for all the treatments, reaching a maximum threshold of 55°C recorded by the treatment (poultry manure (PM) + mixture of residues (wheat straw + olive residues) (MI)). The pH, EC, nitrogen, phosphorus and potassium values increase for all treatments at the end of the test. On the other hand, the MO content and C/N ratio are significantly reduced during the trial, especially for treatments containing poultry manure. The results obtained show that the four prepared composts meet the characteristics of a stable, hygienic product suitable for use in poultry farming.

Determination of gentamicin use in poultry farms in Enugu state, Nigeria, and detection of its residue in slaughter commercial broilers

Background and Aim: Gentamicin is a broad-spectrum antimicrobial used in the treatment of a variety of bacterial infections in poultry, its residue in tissues is known to have a deleterious effect in man when consumed. The study aimed to determine the use of gentamicin by poultry farmers and the presence and occurrence of gentamicin residue in tissues of commercial broilers in Ikpa slaughterhouse Nsukka. Materials and Methods: The study adopted a cross-sectional survey using questionnaire and immunological screening. A total of 108 broilers were sampled, testing 108 each of muscle, liver, and kidney tissues for gentamicin residue using enzyme-linked immunosorbent assay. Results: The questionnaire survey revealed that in the management practices, 55.2% of the farms used intensive deep litter, 21% intensive battery cage, and 23.6% extensive farming system. Twenty-seven (71%) farms reared only broilers, 10.5% layers, and 18.4% both. The feeding management showed that 21.1% of farmers produced their own feed (medicated) while 78.8% used commercial feed, of which 11.5% incorporated antibiotics. Hundred (100%) of the respondents used gentamicin and only 47.3% were aware of the consequences of antimicrobial residue in foods of animal origin, with knowledge of the legislation on the prudent use of antimicrobials in food animals. The occurrence of gentamicin residue in surveyed birds was 65% with tissue distribution as follows: Muscle (44.4%), liver (51.9%), and kidney (59.3%). Conclusion: Poultry farms in the study area make constant use of gentamicin without observing its withdrawal period before sale, and a high percentage of the commercial birds meant for human consumption contains gentamicin residue. Therefore, consumers might be at risk of consuming gentamicin as a parent drug or its metabolites in commercial poultry.

A first approach using micellar electrokinetic capillary chromatography for the determination of fipronil and fipronil-sulfone in eggs.

Fipronil is an insecticide that is not approved in the European Union in food. In 2017, fipronil was involved in a European health alert due to its presence in fresh hen eggs because of an illicit use in poultry farms, so reliable methods are needed to determine fipronil and its main metabolites in these matrixes. In this work, we report the first approach to the study of fipronil and two metabolites, fipronil-sulfone and fipronil-sulfide by CE. MEKC mode was employed using a solution of 50mM ammonium perfluorooctanoate pH 9.0 with 10% (v/v) methanol as background electrolyte. The proposed method was combined with a simple sample treatment based on salting-out assisted LLE (SALLE) using acetonitrile as extraction solvent and ammonium sulfate as salt. The SALLE-MEKC-UV method allowed the simultaneous quantification of fipronil and fipronil-sulfone. Validation parameters yielded satisfactory results, with precision, expressed as relative SD, below 14% and recoveries higher than 83%. Limits of detection were 90µg/kg for fipronil and 150µg/kg for fipronil-sulfone, so in terms of sensitivity further studies of sample treatments allowing extra preconcentration or the use of more sensitive detection, such as MS, would be needed.

Antibiotics use and gentamicin residues in commercial poultry and chicken eggs from Oyo and Lagos States, Nigeria

Nigeria poultry industry is fast growing to meet the demand of the increasing population with overdependence on antibiotics for production leading to consumer safety and public health concerns. The antibiotic use in poultry farms and presence of gentamicin residues in chicken eggs from city markets of Southwest Nigeria were investigated. A semi-structured questionnaire was administered to 45 poultry farmers to determine the patterns of antibiotic use, knowledge of withdrawal periods and food safety implications. Furthermore, 270 egg samples from six retail markets in Oyo and Lagos states were analyzed with ELISA for gentamicin residues. Residue concentrations were compared with Student’s t test and ANOVA (p < 0.05). About 90% of the respondents reported they frequently administered antibiotics, 85% engaged in non-prescribed medication, and about 80% did not observe a withdrawal period before selling the eggs from treated chickens. In addition, 60% and 80% of pooled eggs from Oyo and Lagos states, respectively, contained gentamicin residues with means of 1461 ± 74 and 1350 ± 92 µg/kg, respectively. The mean residues obtained from the two states were higher than the maximum recommended residue limits. High levels of gentamicin residues, from unbridled use of antibiotics in poultry production, detected in retail eggs from markets rendered the eggs unsafe for human consumption. Therefore, regulatory control and veterinary supervision of antibiotic use are advocated to ensure Nigerian consumer protection.

Prevalence, antimicrobial resistance and virulence genes characterization of nontyphoidal Salmonella isolated from retail chicken meat shops in Northern India

The objective of this study was to determine the prevalence of antibiotic resistant nontyphoidal Salmonella in 39 retail chicken meat shops located in 7 towns of North India. Overall, nontyphoidal Salmonella prevalence of 9.43% (70/742) was observed. Highest Salmonella prevalence was observed in Lalkuan (20.99%). Chicken meat samples (40%) showed highest Salmonella prevalence followed by poultry feces (20%). Three serotypes of Salmonella were identified, Salmonella Kentucky (S.Kentucky, 74.29%), Salmonella Virchow (S.Virchow, 17.14%) and Salmonella Typhimurium (S.Typhimurium, 7.14%). All isolates were multidrug resistant (MDR) by disk-diffusion assay, showing resistance to three or more classes of antibiotics. High resistance was observed to tetracycline (100%), erythromycin (100%), nalidixic acid (98.57%), ampicillin (95.71%) and ciprofloxacin (82.86%). Fifty-one (98.08%) S. Kentucky isolates were resistant to ciprofloxacin. Of these, 49 S. Kentucky isolates showed MIC values in the range of 3 to >256 μg/ml. Twenty-nine (41.43%) Salmonella isolates were co-resistant to ciprofloxacin and cefotaxime by disk-diffusion test. The tetA gene was detected in all Salmonella isolates and the blaTEM was the most predominant (25.37%) β-lactam gene. Multiple virulence genes such as sipA, mgtC, sopE1, stn, sopB, fliC, spvC and gipA were also detected. High resistance to quinolone, cephalosporin and tetracycline antibiotics in nontyphoidal Salmonella isolates of poultry origin points towards their extra-label use in poultry farms.

Production performance of Uttara fowl under intensive system of management

A study was conducted to evaluate the growth performance and carcass traits of 44 day-old chicks of Uttara fowl. The experiment was conducted by using the chicks obtained from single hatch. Biweekly body weight was recorded from hatch weight to 12 weeks of age. At the end of trial period, three birds was randomly selected and sacrificed to record the carcass traits. Uttara fowl under farm condition performed better in context of egg weight, hatch weight, biweekly body weight, dressing percentage and has genetic potential for improvement through intensive selection and breeding programme to develop birds with improved efficiency for sustainable use in poultry farming in harsh and cold climatic conditions of hilly region of Uttarakhand.

Antibiotic use in poultry: a survey of eight farms in Thailand.

ObjectiveTo investigate antibiotic use in poultry farms in Thailand and estimate the total amount of antibiotics used annually in Thai production of chicken meat.MethodsIn a single province, we surveyed eight farms in which chickens were raised for meat and interviewed the farms’ owners in 2016. The antibiotic use for each chicken was defined as the amount of antibiotic given to the chicken over its entire lifetime divided by the target weight of the chicken at the time of its slaughter. Assuming that the results were nationally representative, we estimated annual antibiotic use on all Thai chickens raised for meat.FindingsNo use of antibiotics for growth promotion was reported. Five farms raised 1-kg chickens for company A and reportedly used no antibiotics unless the chickens were sick. The other three farms raised 3-kg chickens for company B and reported routine use of antibiotics for prophylaxis. Per kg final weight, each chicken raised for company B was reportedly routinely given a mean of 101 mg of antibiotics – that is, 33 mg of amoxicillin, 29 mg colistin, 19 mg oxytetracycline, 18 mg doxycycline and 2 mg tilmicosin. The total amount of antibiotic used on all Thai chickens raised for meat in 2016 was estimated to be 161 tonnes.ConclusionEach year in Thailand, many tonnes of antibiotics are probably routinely used in raising chickens for meat. Labels on retail packs of meat should include data on antibiotic use in the production of the meat.

Enhancement of Antimicrobial Sensitivity of Salmonella and Escherichia coli Strains Isolated from Chickens Using Silver Nanoparticles in Assiut Governorate

Before the era of complete resistance to antibiotics due to their extensive use in poultry farms, new strategies were discovered, one of them was the use of nanoparticles to enhance the action of antibiotics. Therefore, this study was carried out to find out the antibacterial effect of silver nanoparticles (AgNPs) either separately or in combination with antibiotics. The obtained data showed the antibacterial activity of AgNPs against the tested Escherichia coli and Salmonella strains with MIC level of 0.85 µg/mL. Synergistic effects of AgNPs with antibiotics against E. coli revealed two-fold drop in MIC of ciprofloxacin and amoxicillin. Meanwhile, there was three-fold drop in MIC of gentamicin, cefotaxime and neomycin. Significant finding was observed in the case of the synergism of AgNPs with amoxicillin and gentamicin, the examined E. coli O2 resistant to amoxicillin and gentamicin became sensitive when the antibiotics were combined with AgNPs. It could be concluded that AgNPs can be easily produced by Rosemary aqueous extracts as low-cost, eco-friendly method for generating AgNPs. New generations of bactericidal compounds containing AgNPs could be successfully used in poultry farms for prevention and treating E. coli infections.

Antibiotic use and practices in commercial poultry laying hens in Ogun State Nigeria

Poultry production contributes largely to the Nigerian economy. However, antibiotics misuse by poultry farmers has resulted in multidrug resistance and impeded efficiency of antibiotic treatments in the industry. The study was carried out to assess antibiotic usage in commercial poultry farms. A survey using a structured farm-manager questionnaire was conducted to obtain information on socioeconomic characteristics of the farm owners or managers, the types of antibiotics used, their frequency and purpose of use, the awareness of antibiotics usage and associated consequences, and farmers’ preferred channels of information on prudent antibiotic usage. The respondents’ level of education was university or equivalent (70.9%), high school (12.6%), or no formal education (13.6%). Antibiotics were primarily used for prophylaxis (> 60%) and therapeutics (< 15%). Gentamicin (76.7%) was the most used, followed by tetracycline (64.1%), enrofloxacin and ciprofloxacin (57.3%), furazolidone (49.5%), streptomycin (46.6%), furaltadone (43.7%), chloramphenicol (41.7%), penicillin (25.2%), erythromycin (24.3%), and nalidixic acid (11.7%). About half of the farms used banned furazolidone and chloramphenicol. In total 82 (79.6%) farmers were aware that the use of random antibiotics in the treatment of improperly diagnosed diseases can lead to antibiotic resistance. However 73 (70.9%) were less aware that antibiotics misuse in animals poses a serious threat to humanhealth. Antibiotics were not prudently used by poultry producers, whose lack of awareness may contribute to the issue. It is anticipated that the results of this survey will assist in developing relevant policies toward controlling antibiotic use in poultry farms in Nigeria.

Veterinary drug use in poultry farms and determination of antimicrobial drug residues in commercial eggs and slaughtered chicken in Kaduna State, Nigeria

The occurrence of veterinary drug residues in poultry products in Kaduna state was determined. Information on drug use was obtained from ten layer flocks weekly for 10 weeks. Two hundred commercial eggs, and 378 slaughtered chicken faeces were examined for antibacterial drug residues using a disc diffusion microbial inhibition test with Bacillus cereus ATCC 11778, and Micrococcus luteus ATCC 9341 respectively. All 10 farms used a drug at least once, nine used antibacterial drugs for either prophylaxis, therapy or both. None of the farms observed drug withdrawal period. Two eggs (1%) and 82 (21.8%) of the chicken faeces were positive. Broilers had a significantly higher incidence (33.1%) for antibacterial substances at slaughter ( χ 2, P<0.01) than layers (23.6%) and local chicken (4.8%).

NEW GENERATION FEED ADDITIVE IN POULTRY BREEDING

The analysis of different doses of complex feed additive to poultry ration have been made. The influence of new feed additive with bioresources of the Far East use on productive poultry indexes, on production quality and digestibility of nutritious matters of mixed feed has been studied. Patrinia scabiosifolia, Acanthopanax sesseliflorum, cones of cedare nut husk, brown seaweed, macro- and microelements, vitamins were the object of research. As a result of these researches author presents more effective norms of complex feed additive use in poultry farming of Khabarovsk Kray. The recipe of feed additive from local vegetable resources and complex of vitamins and minerals for use in laying hens nutrition was worked out. The aim of this work was to rise zootechnic and economic indexes of poultry production. The introduction of complex feed additive in ration influenced positively on the digestibility, raised the content of Omega-3 fat acid in egg of laying hens. The increase of Omega-3 fat acid quantity promotes decrease of cholesterol in yolk of egg. In the experimental groups there was a more intensive use of nutrients in diet, mainly due to raw protein, fats, mineral substances – calcium and phosphorus. The use of a new feed additive had a positive effect on the hatchability of eggs, the development of internal organs (heart, liver) and the live weight of chicks obtained from the eggs of chickens in experimental groups. Additionally, the content of mineral substances (calcium, potassium, phosphorus) in the egg of test group was higher than in the control on average by 2.3-5.1%.