Use Of Phytotherapy Research Articles

USE OF PHYTOTHERAPY IN VETERINARY GASTROENTEROLOGY AND DERMATOLOGY: A SYSTEMATIC REVIEW

The impact of the use of herbal medicines and the increase in the number of scientific articles in the area of veterinary medicine is growing, and there is a need to evaluate and condense recent knowledge regarding the use of medicinal plants in veterinary clinics. So, a systematic literature review was carried out with the theme “use of herbal medicine in veterinary gastroenterology and dermatology” based on the literature available between 2018 to 2023 in the PUBMED, SCIELO, and Periódicos Capes databases by retrieving papers written in Portuguese, English and Spanish, accessed electronically. The most common diseases of the gastrointestinal tract (GIT) and skin identified in the review were ulcerative lesions and those caused by infectious pathogens. The herbal medicines used to treat those diseases are based on plants such as copaiba, turmeric, neem, oregano, garlic, calendula, grapes, licorice, among others, whose chemical components are mostly essential oils and phenolic compounds, which present antimicrobial, antioxidant, and anti-inflammatory activities. The present systematic review showed that the use of phytotherapy was effective when carried out with plants producing essential oils and/or phenolic compounds, to be used in different pathologies related to the GIT and skin, in veterinary medicine.

A systematic review on the use of phytotherapy in managing clinical depression

Introduction: The use of medicinal plants in the management of depression, also known as phytotherapy or herbal medicine for depression, is an area of growing interest in the field of mental health and complementary medicine. Methods: This study used a systematic assessment of pertinent literature to assess the effectiveness of medicinal herbs in treating mild to severe depression. A comprehensive literature search was conducted to identify randomized controlled trials (RCTs) that reported data on the intervention, control group, adverse events, outcome measurements, and main findings. A summary and analysis were done on the included research data. Results: We included 23 RCTs investigating the efficacy of herbal medicines, including Crocus sativus, Lavandula angustifolia, Melissa officinalis, and Echium amoenum, in treating depression. In general, saffron showed encouraging outcomes when used to treat mild to severe depression. With no discernible variations in the reported adverse effects, it proved to be equally efficacious as well-known antidepressants like imipramine and fluoxetine. However, it is noteworthy that not all trials yielded favorable results. Conclusion: More investigation is required to fully understand the mechanisms of action, ideal dosage schedules, long-term effects, and relative efficacy of medicinal plants in depressive treatment.

Phytotherapy in the treatment of ashma in children living in the city of Teresopolis-RJ

The use of plants for medicinal purposes for the treatment of diseases is an approach used for many years in a popular way, through teas and other preparations. Based on the promising results that these plants can provide, today there are already several studies and studies that prove the efficacy in the treatments of various diseases through phytotherapy. This study aims to evaluate the use of phytotherapy as an adjunct treatment for asthma symptoms and to point out the problems arising from the lack of information on herbal treatment in general. The data were obtained through a field research conducted at the unifeso pneumopediatric outpatient clinic with the guardians of children with asthma. After a critical literature review, it was evaluated whether the species used have scientific evidence for the expected pharmacotherapeutic activities or whether they are used only based on reports based on popular knowledge. Through this research it was observed that in fact the most used species can promote the relief of asthma symptoms.

Cissus sicyoides phytotherapy as complementary to the treatment of type II diabetes mellitus

Diabetes Melitus (DM) has become a public health issue in recent years. The comorbidities that affect their sufferers lead to a reduction in daily activities, a worsening of quality of life and an increase in mortality as a result of illnesses and complications of the disease. Basic health units, together with their health promotion programs and prevention strategies, have been adapting protocols for comprehensive care for patients with diabetes mellitus, guiding them towards a healthier lifestyle. This work showed the benefits of the complementary use of phytotherapy, specifically Cissus sicyoides in patients with diabetes mellitus, demonstrating hypoglycemic effects in 50% of patients subjected to the use of the dry extract. Adjunct to the treatment of diabetes mellitus II there is a need to implement strategies that reinforce the use of herbal therapies and research needs to be carried out to this end.

Ethnobotanical survey on herbal remedies for the management of type 2 diabetes in the Casablanca-Settat region, Morocco

BackgroundMorocco faces a substantial public health challenge due to diabetes mellitus, affecting 12.4% of adults in 2023. The Moroccan population makes extensive use of phytotherapy and traditional medicine to address the difficulties this chronic condition poses. The aim of this study is to document the use of medicinal plants in traditional medicine for managing type 2 diabetes in the provinces of the Casablanca-Settat region.MethodsThe study employed a semi-structured questionnaire for data collection. A study was conducted between August 1st and September 30th, 2023, and 244 individuals diagnosed with diabetes were invited to take part in the research, all of whom used at least one medicinal plant to manage type 2 diabetes, by visiting primary healthcare facilities in Morocco. The analysis included the use of Relative Frequency of Citation (RFC) to scrutinize the data.ResultsA total of 47 plant species belonging to 25 families were documented. Notably, the Apiaceae, Lamiaceae, and Fabaceae families were frequently mentioned in the context of treating type 2 diabetes in Morocco. Prominent among the cited plant species were Sesamum indicum L., Lepidium sativum L., followed by Foeniculum vulgare Mill., and Rosmarinus officinalis L. Seeds emerged as the plant part most commonly mentioned, with infusion being the prevailing preparation method and oral consumption being the most frequently depicted method of administration.ConclusionThis research underscores the practicality of incorporating traditional medicine into the healthcare framework of the Casablanca-Settat region. The findings not only offer valuable documentation but also have a vital function in safeguarding knowledge regarding the utilization of medicinal plants in this locality. Moreover, they provide opportunities to delve deeper into the phytochemical and pharmacological potential of these plants.

Ethnobotanical study and inventory of medicinal plants used in the treatment of dermatological diseases in southern Benin

The use of phytotherapy in the treatment of skin diseases is becoming more and more widespread in Benin due to the increasing number of dermatological diseases and the cosmetologically demands of the population. This study aimed to identify the medicinal plants used to treat common dermatoses in southern Benin. The methodology is based on ethnobotanical surveys carried out with the help of questionnaires among traditional therapists and medicinal plant sellers of different ages and sexes in some communes of southern Benin. The results obtained allowed us to identify 54 species of medicinal plants used in the treatment of dermatoses. The most frequently cited species include Crateva adansonii, Zanthoxylum zanthoxyloides, Ageratum conyzoides, Cassia alata, and Chromolaena odorata. Leaves are the most commonly used organ (73.29%). Decoction is the most popular method of preparation (95.4%), while bathing is the most commonly recommended method of administration (54.68%). All the information gathered will be used to create a database for future studies in the Beninese pharmacopeia to evaluate the efficacy of these antibacterial plants.

Integral evaluation of thyroid lymphatic region in phytocorrection of hypothyroidism consequences

To date, the integrative involvement of thyroid lymphatic region structures in providing tissue homeostasis in hypothyroidism and during rehabilitation remains a poorly understood problem. The use of phytotherapy is attractive for reducing the effects of hypothyroidism. This issue requires scientific justification during the rehabilitation phase.Material and methods. The work was performed on 160 white Wistar rats using light and electron microscopy according, data of morphometry and statistical matrix analysis. An adequate model of hypothyroidism was created by mercazolil administration. After the mercazolil withdrawal, we investigated the reversibility of thyroid and regional lymph node changes during the rehabilitation period (on 7, 14, 21 days) on the background of bioactive phytocomposition intake and without it. Phytocomposition from laminaria and medicinal plants of Siberia was used at a dose of 0.2 g/kg for three weeks after the cancellation of mercazolil.Results and discussion. The study showed a unidirectional change in thyroid and lymph node structure with a preferential decrease in indicators in hypothyroidism. The value of the total normalized index for the lymphatic region (lymphothyroostat) is negative during the recovery period without phytocorrection. The index has a negative value initially and up to 14 days and becomes positive only by the end of 21 days of the study reflecting the inertia of the structural response of organs. Phytotherapy provides high rationed index rates for lymphothyrostat. The index becomes positive starting at day 14. There is an intensification of compensatory and adaptive reactions at all levels of lymphothyrostat organization, starting with ultrastructural. The lymph node contributes significantly to the provision of homeostasis of the lymph region of the thyroid gland.Conclusions. Reorganization of the lymph node and thyroid gland is interconnected in hypothyroidism. The active role of the lymph node in providing tissue homeostasis in the lymph region of the thyroid is apparent. Thyroid and lymph node function can be enhanced by phytotherapy. Phytocomposition reduces the effects of hypothyroidism. It is a kind of modifier of thyroid and lymph node structure and function

Pregnant women's attitudes towards complementary and alternative medicine and the use of phytotherapy during the COVID-19 pandemic: A cross-sectional study.

Approximately 80% of individuals worldwide use various holistic complementary and alternative medicine (HCAM) methods, including herbal products, to prevent diseases and improve their general health. In this study, it was aimed to investigate complementary and alternative therapy (CAM) and the use of phytotherapy by pregnant women in the COVID-19 pandemic period. This is a cross-sectional and descriptive study. The study included 381 women who applied to a public hospital in Türkiye and used herbal products during this pregnancy. Purposive sampling method was used. The study data were collected through "Identifying Information Form", "Holistic Complementary and Alternative Medicine Questionnaire (HCAMQ)" and "Information Form on the Use of Phytotherapy". In the analysis of the study data, descriptive statistics, the one-way ANOVA and multinomial logistic regression analyses were used. The study was completed with 381 pregnant women. The average age, parity and gestational age of the pregnant women were 28.33±6.09, 2.17±0.95, 29.11±8.87, respectively. It was determined that 37.3% of pregnant women did not know the ingredients of the herbal products they used and 38.8% found them safer than the drugs. HCAMQ total mean score of the pregnant women was calculated as 34.62±16.22. It was found that the pregnant women used garlic the most (65.6%), followed by cumin (38.6%), curcuma (36.2%), and ginger (34.4%). HCAMQ total mean score was found to be lower in the pregnant women who found herbal products safer than drugs (p<0.001), who were not aware of the content of the herbal product they used (p<0.001), and who used herbal products so as to protect against COVID-19 (p = 0.041), to increase their physical resistance (p = 0.022), and to facilitate childbirth (p = 0.002). It was determined that among the pregnant women who knew the content of the herbal products they used (Odds Ratio (OR) 1.122; 1.095-1.149 95%CI; p<0.001) and who did not know (OR 1.114; 1.085-1.144 95%CI; p<0.001), as negative attitude towards HCAM increased, their status of finding traditional drugs safer increased. Among the pregnant women who used herbal products to protect against COVID-19 (OR 1.142; 1.111-1.174 95%CI; p<0.001) and to increase their physical resistance (OR 1.120; 1.094-1.147 95%CI; p<0.001), as negative attitude towards HCAM increased, their status of finding conventional drugs safer increased. In today's world where the use of herbal products and CAM has become widespread, it is important to raise the awareness of pregnant women about the benefits and harms of these practices about which there is inadequate evidence.

Frequency and determinants of phytotherapy use in patients with type 2 diabetes in the Dschang Health District, Cameroon: a cross-sectional study.

phytotherapy is widely used in Africa for the management of many diseases. Data on the use of phytotherapy in people with type 2 diabetes are scarce. We aimed to determine the frequency and factors associated with the consumption/use of phytotherapy products among patients with type 2 diabetes in the Dschang Health District. we conducted a cross-sectional study from January to May 2022, including community-dwelling or hospitalized patients with type 2 diabetes who had lived in the Dschang Health District for at least one year. Informed consent was obtained from all patients. Data were collected using a pre-designed questionnaire. Variables collected included socio-demographic characteristics, diabetes knowledge and practices, and perceptions of care. we included 403 (249 women) patients with type 2 diabetes with a mean (SD) age of 63 (± 14.86) years). Among them, 240 (59.55%) used phytotherapy, either in combination with conventional treatment (168 (41.69%) participants) or not (72 (17.86%) participants), to treat diabetes. The most common reasons for using phytotherapy were easy accessibility and belief in its efficacy. Most patients used both treatments because they thought the combination was more effective. In univariable analysis, we observed a statistically significant association between level of education (p=0.003), socioeconomic level (p<0.001), place of residence (p=0.003), duration of diabetes (p=0.007), and use of phytotherapy. In multivariable analysis, only age between 51 and 60 years (OR: 0.50, 95% CI 0.298 - 0.8521; p=0.01) was associated with the use of phytotherapy. people living with T2D in the Dschang Health District frequently use phytotherapy as an antidiabetic remedy, especially those aged between 51 and 60 years, those with low education level, low socioeconomic level and medium duration of diabetes. There is a need to evaluate its effectiveness in treating diabetes and its adverse effects.

Phytotherapy: knowledge, experience and prescription in oral healthcare. A cross-sectional survey of dental practitioners.

This exploratory survey assessed Brazilian dentists' knowledge, attitudes, and practices regarding phytotherapy. This study used a consecutive sample of registered dentists who were active in clinical practice and attending advanced training for complex oral rehabilitation at a leading dental school in Latin America. Participants responded to a semi-structured 38-item instrument designed to collect data on sociodemographic, professional and health variables, and on their knowledge, use, prescription and opinions of phytotherapy. Quantitative data were analyzed with descriptive and inferential statistics. Qualitative data were described and analyzed with a thematic approach. A total 53 dentists responded the survey (88.3% response rate). Six dentists reported formal education in phytotherapy and 33 had informal knowledge. Twenty-one dentists (39.6%) reported using herbal medicine, and 17 (32.1%) prescribed phytotherapy to their patients, mostly traditional medicinal plants. A logistic regression model showed that post-graduate degree and personal use of phytotherapy were associated with phytotherapy prescription. Opinions on how to improve the rational use of phytotherapy included the need for research evidence, specific academic education, and knowledge dissemination to clinicians. As a clinical recommendation, it was proposed that questions on herbal medicine should be asked during the dental patient's anamnesis. Dentists with post-graduate degrees and personal use of phytotherapy prescribe herbal medicines for their patients in real-world clinics, regardless of formal education on the subject. Qualitative findings indicate the need to implement scientific initiatives.

Satureja hortensis L. and Calendula officinalis L., Two Romanian Plants, with In Vivo Antiparasitic Potential against Digestive Parasites of Swine.

Internal parasitic diseases of swine constitute a major welfare and health concern in low-input livestock farming. Due to an increase in chemical resistance, phytotherapeutic remedies have become an alternative for the prophylaxis and therapy of digestive parasitosis, albeit few remedies have been subjected to scientific validation. Low-input swine farming in Romania has adopted the traditional use of phytotherapy for controlling pathogens in livestock. The current study aimed to assess the antiparasitic potential of Calendula officinalis and Satureja hortensis against digestive parasites of swine in two low-input farms. The fecal samples were collected from sows, fatteners, and weaners, and were tested using the following coproparasitological methods: centrifugal sedimentation, flotation (Willis, McMaster egg counting technique), Ziehl-Neelsen stain modified by Henricksen, modified Blagg method, and in vitro nematode larvae/protozoan oocyst cultures. Six species of digestive parasites were diagnosed, namely Ascaris suum, Trichuris suis, Oesophagostomum spp., Balantioides coli, Eimeria spp., and Cryptosporidium spp., in various combinations, dependent on the swine category. A dose of 140 mg/kg bw/day of C. officinalis and 100 mg/kg bw/day of S. hortensis powders administered for 10 consecutive days revealed a strong antiprotozoal and anthelmintic activity on the aforementioned parasites. The curative efficacy can be attributed to the presence of polyphenols, sterols, tocopherols, and methoxylated flavones. In conclusion, our results indicate that S. hortensis and C. officinalis are promising alternatives to the commercially available antiparasitics, enabling their use as natural antiparasitic products against gastrointestinal parasites in pigs.

Mucoactive therapy in the treatment of sinusitis in patients with post-Covid syndrome

Rhinosinusitis, as in the pre-ovoid era, are still the most common diseases in the practice of an otorhinolaryngologist. According to the results of the analysis of medical documentation conducted at the clinic of the St. Petersburg Scientific Research Institute of Ear, Throat, Nose and Speech, more and more patients associate the diseases of the nasopharyngeal space that have appeared in them and the increase in the number of exacerbations of existing chronic diseases of this area with a coronavirus infection. In this regard, we decided to consider approaches to the treatment of rhinosinusitis by summarizing the available data and considering individual clinical cases of patients with sinusitis and COVID-19. Antibiotic resistance associated with uncontrolled administration of antibacterial drugs when infected with SARS-CoV-2 has also become one of the frequent problems at present. Therefore, our article considers the most relevant method in this case, based on the use of phytotherapy. According to the literature, the most effective phytopreparations are preparations containing standardized myrtol, which reduces the viscosity of the mucus of the paranasal sinuses and nasal cavity, increases the frequency of beating of the cilia of the ciliated epithelium, thereby contributing to an increase in the speed of mucociliary transport. Standardized myrtol also has an anti-inflammatory effect by reducing the concentration of leukotrienes and prostaglandin, neutralizing free OH radicals, inhibiting oxidation processes myrtol reduces toxic effects on the nasal mucosa and paranasal sinuses, and also acts on pathogenic flora as a bacteriostatic drug. Clinical recommendations of the Ministry of Health of the Russian Federation from 2021 according to the treatment of acute sinusitis and the European recommendations of the European Position Paper on Rhinosinusitis and Nasal polyps (EPOS 2020), based on the data of controlled studies presented in them, the use of phytopreparations in the treatment of acute and chronic sinusitis is confirmed both in combination with antibacterial therapy and without antibacterial drugs in combination with irrigation therapy and the use of topical decongestants.

Phytotherapy use for disease control in aquaculture: a review of the last 5 years

Phytotherapy use for disease control in aquaculture: a review of the last 5 years

Access to herbal medicines in Brazil: a cross-sectional study

IntroductionMedicines are essential therapeutic strategies for health systems, thus, it is essential to verify whether the use of herbal medicines is equally distributed among the Brazilian population. MethodsA cross-sectional study was conducted with the Brazilian population. Those over 18 years of age, residing in the country for at least 12 months and with regular access to the internet, were included. The information showed the profile of the individuals, health conditions and characteristics of phytotherapy use. Data were submitted to Binary Logistic Regression (P < 0.05). The research was approved by the Research Ethics Committee of the University of Pernambuco (4880,872). ResultsAmong 1 421 individuals, 727 (51.2%) used herbal medicines. The chance of this use was higher among those who did not identify as female (OR adjusted = 1.4/95% CI = 1.0–1.9; P = 0.028); white (OR adjusted = 1.4/95% CI = 1.0–1.8; P = 0.019); exclusive user of the private care sector (OR adjusted = 1.8/95% CI = 1.1–2.8; P = 0.009); with health problems (OR adjusted = 1.4/95% CI = 1.0–1.9; P = 0.010); and with product prescription (OR adjusted = 7.3/95% CI = 5.4–9.9; P < 0.001). Discussion/ConclusionsThere must be professional training, health education for users and readjustment of public policies to reduce inequalities in the use of herbal medicines.

Use of phytotherapy in treating diabetic foot in older adults: an integrative review

Objective: to identify the scientific evidence on herbal medicines used in treating diabetic foot in older adults. Methods: an integrative review conducted in the Medical Literature Analysis and Retrieval System, SCOPUS, EMBASE, Web of Science and Latin American and Caribbean Health Sciences Literature databases. The Rayyan platform was used as a supporting tool for archiving, organizing and selecting articles. Results: the search resulted in 158 articles. After identifying, selecting, evaluating eligibility, systematization and performing a full reading of the articles, the final sample consisted of 10 articles. We identified herbal medicines derived from Calendula officinalis, Ageratina pichinchensis, Phellodendron chinense, Curcumin phytosome, Capsicum spp., Rehmannia Glutinosa and Astragalus membranaceus. Conclusion: investigations of herbal compounds derived from four plants used in treating diabetic foot in older adults predominated. Considerations for practice: supported by legislation, nurses can guide users interested in using phytotherapy as an accessible solution with proven efficacy.

Use of Phytotherapy in the Treatment of Patients with Erectile Dysfunction

Erectile dysfunction (ED) is one of the most common sexual disorders. In men with a mild stage of ED, it is possible to achieve a therapeutic effect from the use of phytotherapy. The objective: analysis of the effectiveness of the combination of natural compounds in patients with mild ED. Materials and methods. 28 patients (average age – 35±5.3 years) with mild ED participated in the study of the effectiveness of the dietary supplement. All patients were examined before and 1 month after the use of the combination of natural compounds, namely: collection of a detailed medical and sexual history, ultrasound dopplerography of the vessels of the penis, lipidogram, blood glucose level and total testosterone level were measured in the laboratory. The patients received the dietary supplement, which includes zinc citrate, L-arginine, an extract of the fruits of slanky anchovies, an extract of epimedium leaves, an extract of the root of Tongkat Ali, an extract of the root of Korean red ginseng, 1 capsule 2 times a day. Results. During the study, a psychogenic form of ED was found in 12 (42.9%) men, an organic cause of ED in 3 (10.7%), and a mixed form in 13 (46.4%) men. 1 month after the end of the use of the complex, it was established that all indicators of the «International Index of Erectile Function-15» (MIEF-15) questionnaire improved in ED patients. Conclusions. The results of the study showed that the use of the complex significantly improved sexual satisfaction, sexual desire, orgasmic function and quality of life in men with mild ED.

Evaluation of physician candidates' attitudes towards phytotherapy practices with a new scale

IntroductionTo measure the attitudes of physician candidates concerning phytotherapy practices and determine the variables affecting their attitudes. MethodThe study data were collected from 524 physician candidates, who were medical faculty students, using a Scale on Attitudes toward Phytotherapy Practices developed by the researchers. Since there is no scale in the literature that reliably and validly reveals the attitudes of physician candidates toward phytotherapy practices, researchers have developed a new scale. Exploratory and confirmatory factor analyses were used in the process of scale development. Univariate and multiple statistical methods were used for descriptive data analysis. ResultsThe developed scale consisted of three factors, 'trust in phytotherapy', 'phytotherapy use’, and 'predisposition', and 23 items based on the five-point Likert type. It was found that physicians’ attitudes toward 'phytotherapy use' were closer to the positive side, while they were undecided concerning the subscales of 'trust in phytotherapy' and 'predisposition'. The attitudes of first-grade physician candidates toward phytotherapy practices were positive, but more negative attitudes were observed in later grades. ConclusionThese findings show that this newly developed scale is reliable and valid and can be used to measure the attitudes of physician candidates towards phytotherapy. In addition, it is thought that the use of phytotherapy can be extended with more scientific studies using different media channels, raising awareness among individuals about phytotherapy, and adding courses that include phytotherapy and its applications to the medical education curriculum.

Current challenges for the use of phytotherapy: a narrative

Considering the phytotherapy increasing use, there are challenges that must be overcome to applying this therapeutic area in a comprehensive way. In order to explore the main obstacles that limit the application of phytotherapy in the health spheres, the aim of this bibliographic review is to offer a summary of the main issues on this topic. In this narrative literature review, studies about the main challenges for the use and development of phytotherapy were selected. Searches were performed in MEDLINE, PUBMED, SCIENCE DIRECT, SciELO and LILACS. In the selected studies, can be cited how main challenges the raw material and bioactive compounds obtaining, regulatory, safety and efficacy evaluation, issues related to quality control and the health professionals training and knowledge. The global acceptance about the herbal medicines and products based on medicinal plants use shows exponential growth. In order to protect users, some issues must be overcome, how regulatory standardization, health professionals adequacy training and quality assurance, efficacy and safety of the products used in phytotherapy.

MiR-363-3p/PTEN is involved in the regulation of lipid metabolism by genistein in HepG2 cells via ERβ

BackgroundGenistein (GEN) is one of the most well-known phytoestrogens identified in various legumes. Although increasing evidence shows GEN has a potential use in phytotherapy to regulate lipid metabolism, its therapeutic mechanisms have not yet been completely elucidated, especially epigenetic alterations of miRNAs to alleviate lipid accumulation in the liver remains unknown. PurposeTo clarify how GEN modulates the miRNA profile in HepG2 cells and investigate molecular mechanisms of the modulated miRNA on regulating hepatic lipid metabolism. MethodsThe miRNA microarray was performed to compare the miRNAs expression patterns, followed by determining principal miRNA and its target gene associated with hepatic lipid metabolism modulated by GEN. miR-363-3p mimics (mi) and phosphatase and tensin homolog (PTEN)-siRNA were transfected into HepG2 cells and GEN was further treated with the cells for 24 h ResultsGEN induced downregulation of miR-363-3p and upregulation of PTEN, which was a target mRNA of miR-363-3p. The miR-363-3p mi led to an upregulation of sterol-regulatory element-binding protein-1c (SREBP-1c) and its downstream lipid synthesis-related factors in HepG2 cells. In addition, the inhibition of PTEN led to an increase of lipogenesis, which was associated with the AKT/mTOR signal regulation. However, GEN treatment could abrogate the lipogenic effects of miR-363-3p mi or PTEN siRNA. The modulation was associated with estrogen receptor β (ERβ). ConclusionWe discerned a new mechanism that GEN regulated hepatic lipid metabolism by inhibiting miR-363-3p, which could be mediated via ERβ and by targeting PTEN in HepG2 cells. Additionally, GEN reduced hepatic lipid accumulation by regulating PTEN-AKT/mTOR signal. It implicated a protective role of GEN by elucidating its epigenetic modification of the miRNA modulated by ERβ on improving hepatic lipid metabolism and provided novel evidence of the mechanism on targeting miR-363-3p/PTEN in treating hepatic lipid disorders.

Bioestatística: desafio para estudantes e profissionais de saúde

A bioestatística nunca esteve tão em alta como nos últimos anos. Com o surgimento da pandemia de Covid-19 muito se falou de gráficos, tabelas, incidência, taxa de mortalidade, taxa de letalidade. Esses termos navegaram pelas principais mídias e para a população em geral. Entre os alunos de graduação e pós-graduação e até mesmo para profissionais, a bioestatística aparece como um desafio e carrega indagações do tipo: “Para que preciso estudar isso?” ou “Não sou muito bom em cálculos.” O presente artigo pretende discutir, através de embasamento teórico, os motivos pelos quais estudantes e profissionais de saúde têm dificuldades e pouco envolvimento com essa área de estudo. A metodologia foi a busca por artigos, utilizando as palavras-chaves, nas principais bases de dados. O contato com a bioestatística vem sendo introduzido desde as séries iniciais, apresentando-a com um olhar cotidiano, e dando continuidade na graduação, acarreta maior envolvimento e entendimento, desmistificando a bioestatística como uma disciplina apenas para alguns.