Urochloa Humidicola Research Articles

INFLUÊNCIA DA FORMA E POSIÇÃO DA ENCOSTA NAS CARACTERÍSTICAS DO SOLO E NA REGENERAÇÃO NATURAL EM ÁREAS DE PASTAGEM ABANDONADAS

O objetivo desse trabalho foi avaliar a influência do relevo nas características químicas e físico-hídricas do solo e na regeneração natural, em áreas de pastagens abandonadas. Para o estudo foram consideradas oito áreas de relevo movimentado no município de Itaboraí-RJ, em cada uma das áreas foram selecionadas duas topossequências divididas em terços superior, médio e inferior, contemplando as pedoformas côncavas e convexas. Foram instaladas duas parcelas (20 m x10 m) em cada terço, uma para cada pedoforma, nas quais se avaliaram os atributos físico-hídricos e químicos do solo, a regeneração natural e a biomassa aérea da pastagem (biomassa estocada e taxa de rebrota). O terço superior apresentou os menores teores de argila e as características químicas não foram influenciadas pela posição na encosta. Na pedoforma côncava, nas áreas de textura argilosa, obtiveram-se menores valores de soma de bases trocáveis e de carbono, quando comparada à pedoforma convexa, de mesma classe textural. Essa tendência se repetiu, porém, de forma não significativa, nas áreas com textura média. As características físico-hídricas estiveram mais associadas à granulometria do solo que à posição na paisagem. A regeneração natural apresentou poucas espécies e não foram observadas diferenças tanto para a posição na encosta como para a pedoforma. No entanto, a espécie de Poaceae existente na pastagem de cada área foi um indicador mais sensível do potencial de desenvolvimento da regeneração natural do que as características edáficas, com destaque negativo para a paisagem dominada por Urochloa humidicola (Rendle) Morrone e Zuloaga, na qual se encontraram os menores valores de regeneração natural.

Use of Cardboard Disks for Crowning Seedlings in Reforestation

ABSTRACT The present study aimed to evaluate the effect of chemopreventive or water-repellent substances on the durability of cardboard disks used to crown forest tree species. The experiment presented a randomized block design with four repetitions and four sampling times (63, 133, 260 and 365 days) and was installed in Seropedica, RJ, in an area with Urochloa humidicola . Bamboo poles were used to simulate seedlings in the field and define the position of the experimental units. The treatments consisted of in natura cardboard, or treated with CuSO4 solution, pinus shellac, CuSO4 + pinus shellac, CuSO 4 + Sika®, pinus shellac + Sika®, and a second control with manual crowning. The cardboard crowning was effective at suppressing grass growth over all periods evaluated, independent of the treatment. After a year in the field, the cardboard disks treated with CuSO4 solution presented higher physical integrity with only 25% mass loss, against 60% mass loss in the control. The cardboard crowning also reduced soil temperature at the crown area by up to 15 °C in the 0 to 10 cm layer, in comparison with manual crowning. The results of this study indicate that the cardboard crowning method is efficient and has the potential to reduce the cost of weed control in reforestation.

Methanolic extract of Urochloa humidicola on in vitro rumen fermentation

Abstract: The objective of this work was to evaluate the effect of the addition of the methanolic extract of Urochloa humidicola at four different concentrations (0, 75, 150, and 250 g L-1) on the in vitro rumen fermentation of Urochloa brizantha. The following variables were evaluated by the in vitro gas production technique: kinetic parameters; rumen degradation of dry matter; and production and concentration of the methane and carbon dioxide gases and of the acetate, propionate, and butyrate short-chain fatty acids. The addition of the methanolic extract reduces the production of gases generated from the degradation of non-fibrous carbohydrates (fraction A) in 9.55, 6.67, and 13.33%, respectively, at the concentrations of 75, 150, and 250 g L-1, compared with the control group, but it negatively affects the degradation of the dry matter of U. brizantha at the concentrations of 150 and 250 g L-1. The extract shows negative quadratic effect on gas production during 12 and 24 hours of U. brizantha incubation. The extract of U. humidicola reduces methane production and increases short-chain fatty acid production at the concentrations of 75, 150, and 250 g L-1.

Structural and ultrastructural variations in roots of Calopogonium mucunoides Desv. treated with phenolic compounds from Urochloa humidicola (Rendle) Morrone & Zuloaga and phenolic commercial standards

Urochloa humidicola (Rendle) Morrone & Zuloaga is an exotic forage grass cultivated in Brazil. Previous studies have identified different phenolic compounds produced by this species, including flavonoids and p-coumaric acid, which have been well-known as allelochemical compounds. We evaluated the morphology, anatomy and ultrastructure of Calopogonium mucunoides roots exposed to phenolic compounds found in U. humidicola. Leaves of U. humidicola were dried, crushed and submitted to extraction in methanol solution, which was fractionated through partition using organic solvents. In the bioassays, the hydromethanolic leaf extract of U. humidicola, commercial standard solutions of phenols and a control were all tested against the germination and seedling growth of C. mucunoides. The seedlings of C. mucunoides were measured, morphologically described, and the roots were processed according to standard techniques for plant anatomy and electron microscopy. Urochloa humidicola leaf extract inhibited root development, although they stimulated shoot elongation. The p-coumaric acid strongly inhibited plant development, caused root tip necrosis and decreased production of lateral roots, as well as changes in anatomy. Seedlings treated with quercitrin, luteolin and fisetin showed greater lengths, whereas kaempferol negatively affected root growth. Also, U. humidicola extract, p-coumaric acid and kaempferol caused marked changes in root cell ultrastructure. These results suggest a potential phytotoxic effect caused by U. humidicola leaf compounds isolated against C. mucunoides seedlings, although the association of these compounds is suggested to be antagonistic.

Chemical Screening of <i>Urochloa humidicola</i>: Methods for Characterizing Secondary Metabolites and Allelopathic Activity on Forage Legumes

The aim of this study was to identify the main classes of secondary metabolites present in the root and shoot crude extracts and fractions from the forage grass Urochloa humidicola (Rendle) Morrone & Zuloaga and to evaluate the allelopathic effect of these metabolites on forage legumes for intercropping. Phytochemical prospecting analyses, 1H NMR and capillary electrophoresis were performed on extracts of U. humidicola. Allelopathic activity was evaluated in germination of Stylosanthes, Macrotyloma axillare and Lactuca sativa L. (standard) in the presence of crude extracts, isolated saponins, flavonoids and trans-cinnamic acid. The metabolite classes present in the extracts could be determined by the combined use of the tested analytical techniques, but their use alone was usually not sufficient to chemically characterize the species. Capillary electrophoresis was effective in detecting phenolic compounds. Macrotyloma axillare was tolerant to crude extracts of U. humidicola. Saponins and trans-cinnamic acid, but not the flavonoids, reduced germination of the target plants.

An integrative insight on dormancy alleviation in diaspores of Urochloa humidicola (Rendle) Morrone & Zuloaga, a tropical grass with great economic and ecological impact.

Even though exhaustively studied, dormancy alleviation in diaspores of Urochloa humidicola (Rendle) Morrone & Zuloaga remains a mystery. To clarify this, we asked the following question: could dormancy alleviation in diaspores of this species be associated with ageing, GA/ABA balance and remaining structures of the panicoid spikelet? We answer this question using diaspores of U.humidicola cv. BRS Tupi as a biological model, a cultivar with a wide edaphoclimatic range in Neotropical areas and whose diaspores possess 'deep dormancy' when dispersed. We analysed both germination and early plant development using a split-plot model. Our findings demonstrate that dormancy alleviation in diaspores of U.humidicola is a synergic phenomenon driven by crosstalk between age, GA/ABA balance and remaining structures of the panicoid spikelet covering caryopses, since this interaction acts on the dynamics of germination and early plant development. We demonstrate that: (i) spreading germination time is a maternal survival mechanism of this species, which has repercussions for occupational aggressiveness of the species; (ii) remaining structures of the panicoid spikelet covering caryopses are the main modulator of embryo development. These structures control the after-ripening process, which is modulated by some molecular factor. We also highlight that it is necessary to review concepts about dormancy of dispersal units in this grass species.

Nitrogen Management, Nitrogen Use Efficiency, and Seed Yield and Quality of Creeping Signalgrass

Nitrogen (N) fertilization management can affect the seed yield and quality of tropical forage grasses. Studies have shown the effect of N rates on tropical grass seed production, but no study has evaluated the effect of application timing of N on the N use efficiency (NUE) and seed yield and quality of creeping signalgrass [Urochloa humidicola (Rendle) Morrone & Zuloaga, syn. Brachiaria humidicola (Rendle) Schweick.]. Field experiments were conducted over two growing seasons in Santo Anastácio, São Paulo, Brazil, to evaluate the influence of N fertilization timing on plant nutrition, herbage mass, N uptake, NUE, and seed yield and quality of creeping signalgrass ‘Llanero’. The treatments included a control (no N application) and four treatments with N applied at different times (150 kg N ha−1 during the vegetative phase, 150 kg N ha−1 at panicle initiation [prebooting], 100 kg N ha−1 during the vegetative phase plus 50 kg N ha−1 at panicle initiation, and 50 kg N ha−1 during the vegetative phase plus 100 kg N ha−1 at panicle initiation). Topdressed N fertilization increased the concentration of N in the flag leaf by 35.9% (from 12.2 to 16.6 g kg−1) and increased the herbage N uptake by 99.7% (from 72 and 144 kg ha−1). Herbage mass was not affected by topdressed N fertilization. The greatest seed yield (64.2% or 136 kg ha−1 greater than the control, and an average of 22.5% or 64 kg ha−1 greater than the other N treatments) was observed in response to 100 kg N ha−1 applied during the vegetative phase plus 50 kg N ha−1 applied at panicle initiation, which resulted in improved NUE. Seed viability was greater in the treatments where all or most of the N was applied at panicle initiation. In addition, the seed germination percentage was positively affected by N fertilization, regardless of the N application timing.

Special metabolites isolated from Urochloa humidicola (Poaceae).

This study aims to identify special metabolites in polar extracts from Urochloa humidicola (synonym Brachiaria humidicola) that have allelopathic effects and induce secondary photosensitization in ruminants. The compounds were isolated and identified via chromatographic and spectroscopic techniques. The compounds 4-hydroxy-3-methoxy-benzoic acid, trans-4-hydroxycinnamic acid, and p-hydroxy-benzoic acid; the flavonols isorhamnetin-3-O-β-d-glucopyranoside and methyl quercetin-3-O-β-d-glucuronate; and kaempferitrin, quercetin-3-O-α-l-rhamnopyranoside, and tricin were identified in the extract from the leaves of Urochloa humidicola. Two furostanic saponins, namely, dioscin and 3-O-α-l-rhamnopyranosyl-(1-4)-[α-l-rhamnopyranosyl-(1-2)]-β-d-glucopyranosyl-penogenin, as well as catechin-7-O-β-d-glucopyranoside were identified in the methanolic extract obtained from the roots of this plant. This species features a range of metabolites that may be toxic for animals if used in food and may interfere with the growth medium, thereby inhibiting the development of other species.

Tropical forages: morphoanatomy of plants grown in areas with the death of pasture syndrome.

Roots and leaves of Panicum maximum Tanzânia, Mombaça and Massai; Urochloa brizantha Piatã, Marandu and Xaraés; Urochloa humidicola Llanero; Urochloa ruziziensis Ruzizienses; Urochloa hybrida Mulato II and Cynodon nlemfuensis Estrela-roxa were analyzed, seeking to identify characters for better adaptation to the environment that may interfere with digestibility of tissue from the point of view of the rumen in cattle. Were planted ten cultivars in a completely randomized blocks with three repetitions. Was collected vegetative material, which histological slides were prepared from middle third of the sections of roots and leaves. Were observed differences (p>0.05) in the roots: higher volume of epidermal cells (28.62 µm) and overall diameter (1926.41 µm) of Llanero; thicker vascular cylinder (975.09 µm) and more protoxylem (42.25) in Estrela-roxa and occurrence of aerenchyma in cultivars Piatã, Mulato II, Xaraés, Massai, Llanero and Estrela-roxa; Were found higher proportions of bulliform cells in the leaves (121.07 µm) and thicker leaf mesophyll in U. humidicola Llanero (263.63 µm); higher proportion of sclerenchyma fibers in Xaraés and Marandu; lower results for amount of fibers in P. maximum Massai. We conclude that the cultivars Estrela-roxa, Llanero and Massai have greater adaptability to the environment and better nutritional quality.

Cinética ruminal de forrageiras tropicais submetidas ou não a sistema de irrigação

Avaliou-se a composição química e a cinética de fermentação ruminal de quatro espécies forrageiras submetidas ou não à irrigação. As espécies avaliadas foram Urochloa brizantha cv. MG5 (MG-5), Andropogon gayanus (Andropogon), Urochloa humidicola (Humidicola) e Digitaria umfolozi (Digitaria umfolozi). O experimento foi conduzido na fazenda experimental Campus Rural, São Cristóvão, SE. Observou-se diferença significativa (P

Characterization of a Urochloa humidicola methanol extract as a phytogenic additive for ruminants

Characterization of a Urochloa humidicola methanol extract as a phytogenic additive for ruminants

Leaf transcriptome of two highly divergent genotypes of Urochloa humidicola (Poaceae), a tropical polyploid forage grass adapted to acidic soils and temporary flooding areas.

Background Urochloa humidicola (Koronivia grass) is a polyploid (6x to 9x) species that is used as forage in the tropics. Facultative apospory apomixis is present in most of the genotypes of this species, although one individual has been described as sexual. Molecular studies have been restricted to molecular marker approaches for genetic diversity estimations and linkage map construction. The objectives of the present study were to describe and compare the leaf transcriptome of two important genotypes that are highly divergent in terms of their phenotypes and reproduction modes: the sexual BH031 and the aposporous apomictic cultivar BRS Tupi.ResultsWe sequenced the leaf transcriptome of Koronivia grass using an Illumina GAIIx system, which produced 13.09 Gb of data that consisted of 163,575,526 paired-end reads between the two libraries. We de novo-assembled 76,196 transcripts with an average length of 1,152 bp and filtered 35,093 non-redundant unigenes. A similarity search against the non-redundant National Center of Biotechnology Information (NCBI) protein database returned 65 % hits. We annotated 24,133 unigenes in the Phytozome database and 14,082 unigenes in the UniProtKB/Swiss-Prot database, assigned 108,334 gene ontology terms to 17,255 unigenes and identified 5,324 unigenes in 327 known metabolic pathways. Comparisons with other grasses via a reciprocal BLAST search revealed a larger number of orthologous genes for the Panicum species. The unigenes were involved in C4 photosynthesis, lignocellulose biosynthesis and flooding stress responses. A search for functional molecular markers revealed 4,489 microsatellites and 560,298 single nucleotide polymorphisms (SNPs). A quantitative real-time PCR analysis validated the RNA-seq expression analysis and allowed for the identification of transcriptomic differences between the two evaluated genotypes. Moreover, 192 unannotated sequences were classified as containing complete open reading frames, suggesting that the new, potentially exclusive genes should be further investigated.ConclusionThe present study represents the first whole-transcriptome sequencing of U. humidicola leaves, providing an important public information source of transcripts and functional molecular markers. The qPCR analysis indicated that the expression of certain transcripts confirmed the differential expression observed in silico, which demonstrated that RNA-seq is useful for identifying differentially expressed and unique genes. These results corroborate the findings from previous studies and suggest a hybrid origin for BH031.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3270-5) contains supplementary material, which is available to authorized users.

Condição física e desenvolvimento radicular de gramíneas em solo construído após mineração de carvão

Resumo O objetivo deste trabalho foi avaliar a condição física e a potencialidade de uso agrícola de um solo construído após mineração com carvão, além do desenvolvimento radicular de gramíneas tropicais nesse solo, tendo-se considerado diferentes períodos de revegetação. O solo foi construído no início de 2003, e o experimento instalado em novembro/dezembro de 2007. A condição física do solo construído foi avaliada nas parcelas ocupadas por gramíneas perenes, como Urochloa humidicola, Panicum maximum e U. brizantha. Em julho de 2012, foram coletadas amostras de solo com estrutura preservada e não preservada para a determinação dos atributos físico-químicos, nas camadas de 0,00-0,10, 0,10-0,20 e 0,20-0,30 m. Monólitos de solo foram coletados para caracterização do sistema radicular das diferentes gramíneas. Após 58 meses de revegetação, a condição física do solo construído ainda é inadequada ao uso agrícola que envolva práticas mais intensivas de manejo de solo. Entre as camadas avaliadas, somente a de 0,00-0,10 m fornece condições para que o solo cumpra suas funções no ecossistema. Urochloa brizantha mostra-se mais promissora para a recuperação da estrutura do solo na camada de 0,00-0,10 m, e a densidade radicular foi o atributo mais sensível para a diferenciação do desenvolvimento potencial das diferentes espécies nesta camada.

Soil microbiological properties and available nitrogen for corn in monoculture and intercropped with forage

Abstract The objective of this work was to evaluate the effects of corn (Zea mays) in monoculture and intercropped with forage on soil microbiological properties and nitrogen availability under no-tillage in the Cerrado (Brazilian savanna). The experiment was carried out from the 2007/2008 to the 2010/2011 crop season, in a Latossolo Vermelho-Amarelo distrófico (Typic Haplustox). A randomized complete block design with three replicates was used, with the following treatments: corn in monoculture; corn intercropped with Panicum maximum; corn intercropped with Urochloa humidicola; P. maximum in monoculture; and U. humidicola in monoculture. Soil samples were taken at 0.00-0.05, 0.05-0.10, 0.10-0.20, and 0.20-0.30-m soil depths, in the begining and in the end of the last crop season. The intercropping systems of corn increased soil nitrogen availability, but did not alter total nitrogen and organic carbon contents in the soil, when compared to corn in monoculture. Corn intercropped with P. maximum increased soil microbial biomass nitrogen and microbial nitrogen quotient, in comparison to corn in monoculture, as well soil as microbial biomass carbon in the surface soil layer, when compared to corn intercropped with U. humidicola.

Evidence of Allopolyploidy in Urochloa humidicola Based on Cytological Analysis and Genetic Linkage Mapping.

The African species Urochloa humidicola (Rendle) Morrone & Zuloaga (syn. Brachiaria humidicola (Rendle) Schweick.) is an important perennial forage grass found throughout the tropics. This species is polyploid, ranging from tetra to nonaploid, and apomictic, which makes genetic studies challenging; therefore, the number of currently available genetic resources is limited. The genomic architecture and evolution of U. humidicola and the molecular markers linked to apomixis were investigated in a full-sib F1 population obtained by crossing the sexual accession H031 and the apomictic cultivar U. humidicola cv. BRS Tupi, both of which are hexaploid. A simple sequence repeat (SSR)-based linkage map was constructed for the species from 102 polymorphic and specific SSR markers based on simplex and double-simplex markers. The map consisted of 49 linkage groups (LGs) and had a total length of 1702.82 cM, with 89 microsatellite loci and an average map density of 10.6 cM. Eight homology groups (HGs) were formed, comprising 22 LGs, and the other LGs remained ungrouped. The locus that controls apospory (apo-locus) was mapped in LG02 and was located 19.4 cM from the locus Bh027.c.D2. In the cytological analyses of some hybrids, bi- to hexavalents at diakinesis were observed, as well as two nucleoli in some meiocytes, smaller chromosomes with preferential allocation within the first metaphase plate and asynchronous chromosome migration to the poles during anaphase. The linkage map and the meiocyte analyses confirm previous reports of hybridization and suggest an allopolyploid origin of the hexaploid U. humidicola. This is the first linkage map of an Urochloa species, and it will be useful for future quantitative trait locus (QTL) analysis after saturation of the map and for genome assembly and evolutionary studies in Urochloa spp. Moreover, the results of the apomixis mapping are consistent with previous reports and confirm the need for additional studies to search for a co-segregating marker.

Initial Recovery of Organic Matter of a Grass-Covered Constructed Soil after Coal Mining

Revegetation is essential to improve the quality of constructed soils. This study investigated effects of perennial grasses on total organic carbon (TOC) stock, organic matter (OM) fractions and on quality of a recently constructed soil, after coal mining. Soil samples were collected from the 0.00-0.03 m layer two years after the beginning of the experiment. The treatments consisted of Cynodon dactylon cv vaquero (T1); Urochloa brizantha (T2); Panicum maximun (T3); Urochloa humidicola (T4); Hemarthria altissima (T5); Cynodon dactylon cv tifton (T6); bare constructed soil (T8); and natural soil (T9). The treatments with grass species increased the TOC stock by 57 % and increased the OM lability in comparison with T8. Higher C accumulation in the coarse and free light fractions and a higher C management index were observed in T2 and T3, indicating greater suitability of these species for the initial recovery of OM of the constructed soil.

Reclamation of a Degraded Coal-Mining Area with Perennial Cover Crops

ABSTRACT: Studies addressing the potential of grass roots in improving the structural quality of constructed minesoils are not frequent in the literature, although they are essential for understanding the re-establishment of soil functions in the environment. The objective of this study was to quantify the root attributes of the species Urochloa humidicola, Panicum maximum, and Urochloa brizantha and relate them to the physical properties of a constructed minesoil in reclamation of an area degraded by coal mining. The study was [...]

Effects of Urochloa humidicola on Plant Diversity in Native Grasslands in a Neotropical Wetland

Studies on species invasion in wetlands are fundamental to understanding the consequences of the introduction of exotic plants, which are often utilized in grasslands. The loss of native plant diversity is of increasing concern. We evaluated the effect of Urochloa humidicola on floristic similarity between native and cultivated grasslands and the differences in spatial distributions between species. To analyze the effect of seasonality, we collected samples at the end of flood and dry periods. In native grasslands and another area cultivated with U. humidicola, samples were collected at three elevations (bottom; intermediate; and top). We identified 90 species and observed a high similarity between the cultivated and native grassland during the flood period; both grasslands exhibited significant differences during the drought period. This difference was due to an increased presence of grasses, mainly U. humidicola, in the cultivated grassland. Increased similarity between bottom and intermediate elevations was due to an increased proportion of aquatic macrophytes. In contrast, the top elevation differed due to the contribution of terrestrial species. We conclude that the native and cultivated grasslands exhibit differences in vegetation composition and are primarily structured by seasonal flooding, and small variations in elevation promote zonation within the plant community.

Microsatellite markers for Urochloa humidicola (Poaceae) and their transferability to other Urochloa species.

BackgroundUrochloa humidicola is a warm-season grass commonly used as forage in the tropics and is recognized for its tolerance to seasonal flooding. This grass is an important forage species for the Cerrado and Amazon regions of Brazil. U. humidicola is a polyploid species with variable ploidy (6X–9X) and facultative apomixis with high phenotypic plasticity. However, this apomixis and ploidy, as well as the limited knowledge of the genetic basis of the germplasm collection, have constrained genetic breeding activities, yet microsatellite markers may enable a better understanding of the species’ genetic composition. This study aimed to develop and characterize new polymorphic microsatellite molecular markers in U. humidicola and to evaluate their transferability to other Urochloa species.FindingsA set of microsatellite markers for U. humidicola was identified from two new enriched genomic DNA libraries: the first library was constructed from a single sexual genotype and the second from a pool of eight apomictic genotypes selected on the basis of previous results. Of the 114 loci developed, 72 primer pairs presented a good amplification product, and 64 were polymorphic among the 34 genotypes tested. The number of bands per simple sequence repeat (SSR) locus ranged from 1 to 29, with a mean of 9.6 bands per locus. The mean polymorphism information content (PIC) of all loci was 0.77, and the mean discrimination power (DP) was 0.87. STRUCTURE analysis revealed differences among U. humidicola accessions, hybrids, and other Urochloa accessions. The transferability of these microsatellites was evaluated in four species of the genus, U. brizantha, U. decumbens, U. ruziziensis, and U. dictyoneura, and the percentage of transferability ranged from 58.33% to 69.44% depending on the species.ConclusionsThis work reports new polymorphic microsatellite markers for U. humidicola that can be used for breeding programs of this and other Urochloa species, including genetic linkage mapping, quantitative trait loci identification, and marker-assisted selection.

Esterase polymorphism for genetic diversity analysis of some accessions of a native forage grass, Mesosetum chaseae Luces, from the Brazilian Pantanal

The aim of the present study was to estimate the genetic diversity within the samples of Mesosetum chaseae from the Embrapa Pantanal Germplasm Bank (BAG) and assess how they are genetically structured to guide proposals to: 1) identify native forages for further testing to measure their suitability for sowing in conjunction with or as an alternative to exotic forages, mainly Urochloa humidicola ; and 2) improve the species M. chaseae with samples that are maintained in the BAG. Isozyme α- and β-esterases were analyzed in 10 accessions collected from different locations in the Nhecolândia sub-region of the Pantanal, and maintained in the BAG. Accessions A11, which showed the highest effective number of alleles, and A32 with the highest average values of expected and observed heterozygosity, were identified as warranting further study as possible options for sowing as pasture forages, as well as for use in recovering poor and degraded areas in the Pantanal region. A high level of population differentiation was detected among the 10 accessions, indicating that they form genetically structured populations and that all accessions are important samples of M. chaseae , which should be maintained in the BAG. Crosses between sample plants with the highest genetic distances are recommended to implement improvement plans with a prospect of broadening the genetic base of the species. Keywords: Genetic polymorphism, genetic resources, grama-do-cerrado, isoesterases, Poaceae. DOI: 10.17138/TGFT(3)194-204