Urochloa Decumbens Research Articles

Immunocytochemistry and Density Functional Theory evidence the competition of aluminum and calcium for pectin binding in Urochloa decumbens roots

Root growth is reduced in soils with low pH [H+] and abundant soluble aluminum [Al3+], which can be a consequence of the interaction between Al3+ and cell wall composition. The competition between Al3+ and Ca2+ toward binding to pectin molecules was evaluated in roots of Urochloa decumbens, an African grass highly adapted to acidic Al-rich soils. Variations in the composition and distribution of pectins can change the extensibility, rigidity, porosity, and adhesive properties of plant cell walls, which were tested in seedlings of U. decumbens exposed to pH 3.5, 4.5 and 5.8 and to 0, 80, 160 and 320 μM of Al3+ for 80h. Root growth corroborated that U. decumbens is very tolerant to soil acidity, with effective reduction of root growth only at pH 3.5. Immunocytochemical approaches demonstrated variations in pectin composition induced both by Al3+ and by H+ in root tissues and zones. Based on the usual linkage between Ca2+ and pectins, Density Functional Theory (DFT) analyses indicated that Al3+ bound easier to pectins than Ca2+ did, leading to the formation of more Al3+-pectate complexes than Ca2+-pectate complexes, which resulted in higher rigidity of cell walls, and hampered cell extension.

Mapping Productivity and Essential Biophysical Parameters of Cultivated Tropical Grasslands from Sentinel-2 Imagery

Nitrogen (N) is the main nutrient element that maintains productivity in forages; it is inextricably linked to dry matter increase and plant support capacity. In recent years, high spectral and spatial resolution remote sensors, e.g., the European Space Agency (ESA)’s Sentinel satellite missions, have become freely available for agricultural science, and have proven to be powerful monitoring tools. The use of vegetation indices has been essential for crop monitoring and biomass estimation models. The objective of this work is to test and demonstrate the applicability of different vegetation indices to estimate the biomass productivity, the foliar nitrogen content (FNC), the plant height and the leaf area index (LAI) of several tropical grasslands species submitted to different nitrogen (N) rates in an experimental area of São Paulo, Brazil. Field reflectance data of Panicum maximum and Urochloa brizantha species’ cultivars were taken and convoluted to the Sentinel-2 satellite bands. Subsequently, different vegetation indices (Normalized Difference Vegetation Index (NDI), Three Band Index (TBI), Difference light Height (DLH), Three Band Dall’Olmo (DO), and Normalized Area Over reflectance Curve (NAOC)) were tested for the experimental grassland areas, and composed of Urochloa decumbens and Urochloa brizantha grass species, which were sampled and destructively analyzed. Our results show the use of different relevant Sentinel-2 bands in the visible (VIS)–near infrared (NIR) regions for the estimation of the different biophysical parameters. The FNC obtained the best correlation for the TBI index combining blue, green and red bands with a determination coefficient (R2) of 0.38 and Root Mean Square Error (RMSE) of 3.4 g kg−1. The estimation of grassland productivity based on red-edge and NIR bands showed a R2 = 0.54 and a RMSE = 1800 kg ha−1. For the LAI, the best index was the NAOC (R2 = 0.57 and RMSE = 1.4 m2 m−2). High values of FNC, productivity and LAI based on different sets of Sentinel-2 bands were consistently obtained for areas under N fertilization.

Effects of animal manure upon growth of Cerrado plants

Through excretion, animals return to the soil part of the nutrients they ingest. This input can be an alternative to artificial inorganic fertilization aiming at increasing soil nutrient concentrations in degraded areas that are prone to restoration. In nutrient-poor soils, the addition of animal manure can promote increases in soil fertility, but studies assessing its effects upon plant communities are meager. This study aims at checking whether the addition of manure originated from livestock promotes plant growth increase and enzyme activity in Cerrado native plants of different functional groups as a way to subsidize processes of environmental recuperation in degraded areas using organic fertilization of an easily available source, as animal manure. The experiment was conducted in a screenhouse through a completely randomized design with four manure and six species treatments with three replicates. Manure treatments used were control, cattle, sheep and goat manure. The plant species selected belonged to main Cerrado herbaceous functional groups – two naturalized grasses, two alien invasive grasses, a legume herb and a Malvaceae herb. Phosphorus and nitrogen concentrations, and N:P ratios varied according to the type of manure. Soil organic matter content, phosphorus and potassium concentrations increased upon manure addition. Different manure types promoted various effects in height, biomass and acid phosphatase activity amongst the plants. Goat and cattle manure positively influenced Urochloa decumbens, an exotic invasive grass living in the Cerrado, whereas sheep manure addition promoted increase of non-invasive grasses. These results show that animal manure can promote better nutrient conditions for plants living in degraded areas, but the selection of the manure type for application in restoration projects aiming at aiding plant growth in Cerrado degraded areas will depend on the purpose of the project because different dung types benefit different plant functional groups.

Mycorrhizal networks facilitate the colonization of legume roots by a symbiotic nitrogen-fixing bacterium.

Arbuscular mycorrhizal fungi (AMF) absorb and translocate nutrients from soil to their host plants by means of a wide network of extraradical mycelium (ERM). Here, we assessed whether nitrogen-fixing rhizobia can be transferred to the host legume Glycine max by ERM produced by Glomus formosanum isolate CNPAB020 colonizing the grass Urochloa decumbens. An H-bridge experimental system was developed to evaluate the migration of ERM and of the GFP-tagged Bradyrhizobium diazoefficiens USDA 110 strain across an air gap compartment. Mycorrhizal colonization, nodule formation in legumes, and occurrence of the GFP-tagged strain in root nodules were assessed by optical and confocal laser scanning microscopy. In the presence of non-mycorrhizal U. decumbens, legume roots were neither AMF-colonized nor nodulated. In contrast, G. formosanum ERM crossing the discontinuous compartment connected mycorrhizal U. decumbens and G. max roots, which showed 30-42% mycorrhizal colonization and 7-11 nodules per plant. Fluorescent B. diazoefficiens cells were detected in 94% of G. max root nodules. Our findings reveal that, besides its main activity in nutrient transfer, ERM produced by AMF may facilitate bacterial translocation and the simultaneous associations of plants with beneficial fungi and bacteria, representing an important structure, functional to the establishment of symbiotic relationships.

Fire cues and germination of invasive and native grasses in the Cerrado

Fire-related cues may enhance seed recruitment in flammable ecosystems and differences in fire-germination responses of invasive vs. native species can drive the invasion process. We evaluated the effects of heat shock and smoke water solution treatment on seed germination of invasive (Melinis minutiflora, Urochloa decumbens, U. brizantha) and native grasses (Axonopus pressus, Aristida setifolia, Gymnopogon foliosus) of the Cerrado. The effect of fire temperature was tested by exposing seeds to heat shock treatments of 60, 100, and 200 °C for one minute, comparing them to untreated seeds. The effect of smoke was tested by soaking seeds for 24-h in an aqueous smoke comparing them to seeds soaked in distilled water. Differences among treatments were evaluated by ANOVA and randomization tests. None of the tested species had germination stimulated by the temperature or smoke. Both species of Urochloa experienced decreased viability with increasing temperature, while seeds of native species and M. minutiflora tolerated heat shock up to 200 °C. The invasive grasses usually germinated faster than the native grasses. Germination time is therefore a crucial trait driving the invasion process, irrespective of fire-related cues. Other seed traits (e.g. dormancy) and higher resprouting capacity may help invasive species to persist in frequently burned savannas.

Selection strategies for increasing the yield of high nutritional value leaf mass in Urochloa hybrids

Experimental genotype selection in a forage crop cultivar development program is complex because the ultimate aim is increase performance and sustainability in animal production. The objectives of this study were (1) to identify traits with greater direct and indirect effects on yield of high nutritional value leaf mass (NLM) in Urochloa sp. hybrids and (2) to assess indirect gain from selection for these traits with greater effects through selection indexes and by genotype by yield*trait (GYT) biplot analysis using NLM as a basic variable. We evaluated 96 interspecific hybrids, from a gene pool among Urochloa ruziziensis, Urochloa brizantha and Urochloa decumbens species, in an experiment laid out in a randomized complete block design. A series of agronomic and nutritional value traits were measured. Path analysis and GYT were performed using NLM as the basic variable, and different strategies using selection indexes were adopted. The leaf dry matter and field green weight (FGW) traits exhibited greater direct effects on NLM. All selection strategies proved to be effective in obtaining gains in the NLM variable. GYT analysis and the selection index with weights corresponding to the relative direct effects to each trait on the NLM were the strategies that resulted in a greater correlated response for NLM. Indirect selection for NLM via FGW or the index with the FGW and regrowth capacity traits proved to be viable strategies for selection of Urochloa genotypes in the initial stages of the breeding cycles due to their practicality and lower requirement regarding traits to be measured.

Synergistic impacts of co‐occurring invasive grasses cause persistent effects in the soil‐plant system after selective removal

AbstractHuman influence on the environment is so extensive that virtually all ecosystems on the planet are now affected by biological invasions. And, often, ecosystems are invaded by multiple co‐occurring non‐native species. Hence, it is important to understand the impacts these invasions are producing on biodiversity and ecosystem processes.Here, we present results of a 2‐year long field experiment where we tested the effects of co‐occurring invasive C4African grasses in a Cerrado area in central Brazil. We compared plant and arthropod communities, plant biomass, and soil nitrogen dynamics and soil chemical characteristics across five experimental treatments:Urochloa decumbensremoval;Melinis minutifloraremoval; bothU.decumbensandM.minutifloraremoval;U.decumbensandM.minutiflorainvaded plots; and uninvaded Cerrado. We hypothesized that selective removal of invasive grasses would have distinct effects on the native ecosystem structure and functioning. We expected that each invasive grass would produce a different type of impact on the native ecosystem and that their impacts would be synergistic when co‐occurring.Removal ofM.minutifloradoubled native plant diversity and biomass when compared to invaded plots, whereas removal ofU.decumbensdid not alter these parameters. Cerrado plots had four times more plant species than plots cleared of invasives. Removal of invasive grasses did not affect the species richness or community composition of soil epigeal fauna. Cerrado soils had lower fertility, organic matter content and pH than invaded soils. The effects were generally higher when both invasive grasses were removed, suggesting impacts were synergistic, butM.minutiflorahad greater effects on plants and soils thanU.decumbens. Both invasive species produced negative impacts, but a single species was the main driver. We also detected persistent effects of the invasive grass species on the ecosystem after 2 years of removal.We conclude that invasive species of the same functional group have similar types of effects in native ecosystems, but the magnitude of impact was largely dependent on invasive species biomass and cover. Where multiple invasive species are present, research and management of invaded ecosystems should tackle the interacting effects of co‐occurring invaders.

Potential of Grasses in Phytolith Production in Soils Contaminated with Cadmium.

Cadmium (Cd) is a very toxic heavy metal occurring in places with anthropogenic activities, making it one of the most important environmental pollutants. Phytoremediation plants are used for recovery of metal-contaminated soils by their ability to absorb and tolerate high concentrations of heavy metals. This paper aims to evaluate the potential of grasses in phytolith production in soils contaminated with Cd. The experiments, separated by soil types (Typic Quartzipsamment, Xanthic Hapludox and Rhodic Hapludox), were conducted in a completely randomized design with a distribution of treatments in a 3 × 4 factorial scheme with three replications. The factors were three grasses (Urochloa decumbens, Urochloa brizantha and Megathyrsus maximus) and four concentrations of Cd applied in soils (0, 2, 4 and 12 mg kg−1). Grass growth decreased and increased Cd concentration in shoots of grasses with the increased Cd rates in soils. The toxic effect of Cd resulted in production and Cd occlusion in phytoliths produced in shoots of the grasses. Grasses showed potential for phytolith production, independent of soil type, providing phytoextraction of Cd in phytoliths. Megathyrsus maximus was the grass with the highest tolerance to Cd, evidenced by higher production and Cd capture in phytoliths for the evaluated soils. Phytolith production by grasses in Cd-contaminated soils is related to genetic and physiological differences of the evaluated grasses and Cd availability in soils.

Effects of nitrogen fertilisation and irrigation on seed yield and yield components of signal grass (Urochloa decumbens)

The effects of nitrogen (N) fertiliser rate and irrigation on seed yield and its components were evaluated for signal grass (Urochloa decumbens (Stapf) R.D. Webster; syn. of Brachiaria decumbens Stapf) cv. Basilisk in a field experiment in Umuarama, Paraná, Brazil. Two water regimes (irrigated and non-irrigated) and four nitrogen (N) fertiliser rates (0, 25, 50 and 75 kg ha–1) were applied to perennial signal grass crops in a split-plot randomised complete block design with three replications. In two consecutive harvests, favourable rainfall resulted in irrigation having limited influence on most measurements, and the combined application of irrigation and N fertiliser did not improve seed yield. Compared with the nil N, the highest N application rate significantly increased seed yield for the first crop (266 vs 498 kg ha–1) and the second crop (104 vs 286 kg ha–1). Nitrogen fertilisation significantly increased number of seed per area, reproductive tiller density and plant biomass at harvest for the first and second crops. Harvest index, 1000-seed weight, reproductive tiller weight, number of spikelets per panicle and number of seeds per panicle were unaffected by N rate. Harvest index ranged from 1.10% to 3.63% and 1000-seed weight from 2.15 to 3.36 g. There were no treatment effects on number of days to flowering or anthesis. Fertilisation with 75 kg N ha–1 for the first crop and 50–75 kg N ha–1 for the second crop maximised signal grass seed yield.

Using additive indices and principal components to select sexual genitors and hybrids of Urochloa decumbens

Abstract The selection of sexual genitors in Urochloa decumbens breeding is dependent upon the performance of their progeny simultaneously for several traits. Thus, our objectives were to (i) compare the efficiency of indices to select genitors of U. decumbens, (ii) evaluate the genetic gains obtained through selection intensities, and (iii) evaluate the multivariate pattern of progenies through principal components analysis (PCA). For this purpose, 1415 hybrids from 75 progenies of full siblings were evaluated at Embrapa Beef Cattle (Brazil) using seven cuts for dry matter production, regrowth, protein, fiber, lignin, and percentage digestibility. Statistical analyses were performed using mixed models and PCA. The direct selection for dry matter production provided a 37.51% genetic gain. Agronomic traits using indices provided greater gains. Genitors selected using PCA Biplot were similar to selections using indices. Indices and PCA were proven to be an excellent tool to select multi-traits in U. decumbens.

Production and nutritive value of pastures in integrated livestock production systems: shading and management effects

This study aimed to evaluate the production characteristics of pastures in integrated livestock production systems. For that, an experiment was carried out in São Carlos, SP, Brazil, from 2013 to 2015. Forage development, production and nutritive value were evaluated in five beef cattle production systems: extensive continuous stocking (Urochloa decumbens) = EXT; intensive = INT; crop-livestock = iCL; livestock-forest = iLF and crop-livestock-forest = iCLF. Rotational stocking pastures in INT, iCL, iLF and iCLF systems were established with Urochloa brizantha cv. BRS Piatã. In iCL and iCLF, pastures were renovated by resowing the grass simultaneously with corn. In iLF and iCLF, eucalyptus (Eucalyptus urograndis clone GG100) was planted in Apr 2011 in single rows with 15 × 2 m spacing. In the 2013/2014 crop season, INT, iCL, and iCLF pastures were more productive than in iLF and EXT. Shading increase in the 2014/2015 season reduced pasture production in iLF and iCLF, compared with INT and iCL, but increased crude protein content and digestibility. In the shaded systems, pasture production was affected by proximity to trees, mainly due to reductions in solar radiation transmission. The principal component analyses showed that forage accumulation and leaf area index were associated with the position in the middle of the inter-row, and nutritive value was associated with the position at 1.5 m from the trees. In iCLF, solar radiation transmission greater than 60 % maintained forage accumulation similar to iCL, while in iLF, it reduced forage accumulation, evidencing that pasture renovation minimized shading effects in these systems.

Weed Interference in Soybean Crop Affects Soil Microbial Activity and Biomass

ABSTRACT: Weeds and microorganisms interacting with their rhizosphere may influence nutrient absorption, which is an important factor for plant competition. The purpose of this study was to evaluate the microbiological activity, inorganic phosphorus solubilization (Pi) and acid phosphatase in the cultivated soil, in a combination of soybean (Glycine max) plants and weeds. Soybeans were cultivated in monoculture and in competition with Bidens pilosa, Brachiaria decumbens (Syn. Urochloa decumbens) and Eleusine indica, under two conditions: a) plants competing without contact between the roots b) plants competing with contact between the roots. A nylon screen with a 50 µm mesh was added to prevent contact between the roots of the species in competition so that the substratum could be separated in the vase. The experiment was conducted in randomized blocks, with four replications. The soybeans in competition with weeds led to lower oxidation of organic matter per unit of microbial biomass, resulting in a lower metabolic quotient, compared with the soybean monoculture. The contact between soybean roots and B. pilosa, B. decumbens and E. indica maintained a strong influence, raising the solubilization of Pi, respectively valued at 51, 39 and 31% in relation to the cultivation of each species with a nylon screen. Microbiological activity, inorganic phosphorus solubilization and acid phosphatase were altered by plant species, combinations of weeds and soybean plants in competition; by root contact in some cases. Thus, the microbiological activity of the soil can influence competition strategies and plant development.

Homeopathic additives and virginiamycin® in grazing beef cattle

The aim of this study was to evaluate the effects of homeopathic additives and the non-ionophore antibiotic virginiamicin® on intake, digestibility, metabolic characteristics and carcase characteristics in Nellore bulls under supplemented grazing. Twelve uncastrated Nellore bulls were used, with an initial mean weight of 346.04 ± 13.33 kg and initial age of 20.00 ± 2.00 months. The animals were kept in individual paddocks of Urochloa decumbens Stapf. (syn. Brachiaria) and divided into two treatments: Lipomax® - supplement concentrate with homeopathic additives (Convert H®, Sodo 100®, Figotonus®) and a non-ionophore antibiotic (Virginiamycin®); Control - supplement concentrate containing the same protein content, but with no additives. The experiment was carried out in a completely randomised design. Intake, the apparent digestibility of the dietary constituents and the metabolic characteristics of the bulls were not affected by supplementation with additives (P>0.05). Lipomax® improved weight gain in the animals (P 0.05). Supplementation with homeopathic additives and virginiamicina® had a positive effect on performance and on hot and cold carcase weight in grazing Nellore bulls.

Pasture recovery using Stylosanthes cv. Campo Grande: effect on soil quality

The objective of this study was to study the physical attributes and the organic matter of a Red-Yellow Oxisolcultivated with Urochloa decumbens pasture in recovery with different introducing ways of Stylosanthes cv. Campo Grande. Theexperiment was conducted at the Agency for Agribusiness Technology of São Paulo (APTA), in the municipality of Andradina/SP, between October 2016 and September 2017. The experimental design was the randomized complete block design withfour replicates and the treatments were composed of six strategies (partial desiccation, total desiccation, scarification, “rome”harrowing and plowing + harrowing.) in the no-tillage legume in the pasture, in addition to the control. Stability of aggregates,flocculation, soil porosity, organic matter and soil texture were evaluated in three layers: 0-0.10; 0.10-0.20 and 0.20-0.40 m.Soil organic matter content was higher in the topsoil layers (from 15.75 to 17.75 mg dm-3) and decreased with depth (from10.50 to 11.50 mg dm-3). The indicated macroporosity was below the value considered ideal for the plants development. Studiedtreatments did not influence the organic matter content, porosity and soil density. Soil quality was influenced up to 0.20 m with theintroduction of stylosanthes, regardless of the adopted management.

Hormetic effect of glyphosate on Urochloa decumbens plants

Urochloa decumbens plants may be reached by herbicide drift from applications of glyphosate from neighboring areas or by variations during applications. Considering the different phenological stages and size of plants in these areas, the amount of active ingredient that reaches the plants probably varies. The objective of this study was to evaluate the effects of the application of different doses of glyphosate on U. decumbens plants. Two greenhouse experiments were conducted with two replications at different times. The first experiment evaluated the biological response of U. decumbens plants to glyphosate doses (0, 2.81, 5.63, 11.25, 22.5, 45, 90, 180, 360, 720, and 1,440 g a.e. ha−1), with six replications. The second experiment evaluated the response of U. decumbens plants to the application of a selected low dose of 11.25 g a.e. ha−1. Evaluations of injury were performed at 0, 7, 14, and 21 days after application, and dry weight of plants was determined for each evaluation period. U. decumbens plants increased in dry weight when using the glyphosate dose of 11.25 g a.e. ha−1. However, plants had different responses to the application of this low dose. It can promote both stimulation and inhibition of plant growth.

Weed hosts of Meloidogyne spp. and the effect of aqueous weed extracts on egg hatching

SummaryWeeds have a great economic impact on agricultural production because they compete with crops for resources and are alternative hosts for pests, microbial pathogens and plant‐parasitic nematodes. This study aimed to investigate the susceptibility of weeds to the root‐knot nematodes Meloidogyne javanica and Meloidogyne incognita and assess the effect of aqueous weed extracts on M. javanica egg hatching. Four experiments were conducted, two for each nematode species. Weeds were inoculated with 2000 nematode eggs and grown for 60 days under greenhouse conditions. Soyabean cv. Monsoy 7110 was used as control. The weeds Ipomoea grandifolia, Solanum americanum, Digitaria horizontalis, Amaranthus deflexus, Sorghum halepense and Commelina benghalensis were susceptible to M. javanica and M. incognita in at least one experiment (reproduction factor, RF >1). Crotalaria juncea and Eleusine indica were susceptible to M. incognita in one experiment, and Digitaria insularis, Sida rhombifolia, Bidens pilosa, Urochloa decumbens, Crotalaria breviflora, Cenchrus echinatus, Crotalaria ochroleuca and Crotalaria spectabilis were immune or resistant (RF <1 or RF = 0). Alternanthera tenella, C. juncea, S. rhombifolia, C. ochroleuca, C. spectabilis, C. breviflora, B. pilosa, E. indica, U. decumbens and C. echinatus were resistant or immune to M. javanica (RF <1 or RF = 0). Compared with the control (water), all weed extracts reduced M. javanica egg hatch. Our results highlight the importance of weed control in agricultural systems, as nematodes can survive and multiply in weed roots during the off‐season. Weed leaves and shoots, however, may be an interesting source of compounds with nematicidal activity.

Understanding the delayed expression of Al resistance in signal grass (Urochloa decumbens).

Signal grass (Urochloa decumbens) is a widely used pasture grass in tropical and sub-tropical areas due to its high aluminiun (Al) resistance. However, the underlying mechanisms conferring this resistance are not clearly understood. The Al concentrations of bulk root tissues and the intracellular compartment were examined, including the impact of a metabolic inhibitor, carbonyl cyanide m-chlorophenyl hydrazone (CCCP). Next, we examined changes in the properties of signal grass root tissues following exposure to toxic levels of Al, including the cell wall cation exchange capacity (CEC), degree of methylation and concentrations of cell wall fractions. Although signal grass was highly resistant to Al, there was a delay of 24-48 h before the expression of this resistance. We found that this delay in the expression of Al resistance was not related to the total Al concentration in the bulk apical root tissues, nor was it related to changes in the Al bound to the cell wall. We also examined changes in other properties of the cell wall, including the CEC, degree of methylation and changes in the concentration of pectin, hemicellulose and cellulose. We noted that concentrations of intracellular Al decreased by approx. 50 % at the same time that the root elongation rate improved after 24-48 h. Using CCCP as a metabolic inhibitor, we found that the intracellular Al concentration increased approx. 14-fold and that the CCCP prevented the subsequent decrease in intracellular Al. Our results indicate that the delayed expression of Al resistance was not associated with the Al concentration in the bulk apical root tissues or bound to the cell wall, nor was it associated with changes in other properties of the cell wall. Rather, signal grass has an energy-dependent Al exclusion mechanism, and this mechanism requires 24-48 h to exclude Al from the intracellular compartment.

Fertilization with liquid swine manure increases productivity and improves the quality of Urochloa decumbens

This study aims at assessing the influence of mineral fertilization and biofertilizer from liquid swine manure (LSM) in productivity, levels of crude protein (CP), neutral detergent fiber (NDF) and Acid Detergent Fiber (ADF) of Urochloa decumbens. The experiment was carried out in a red Latosol under a Urochloa decumbens pasture, in a state of degradation with loss of vigor and forage productivity. The delineation chosen for this study was randomized blocks with six treatments (control without fertilization, control with use of mineral fertilizer and four unique concentrations of liquid swine manure biofertilizer (61 m3 ha-1, 121 m3 ha-1, 181 m3 ha-1, 241 m3 ha-1)). The application of residue has been split in two times on the surface of the soil, by means of a distributor of liquid waste with a fan-shaped application. Yield and bromatological features were evaluated for two years. Fertilization with LSM can fully replace mineral fertilizing in Urochloa decumbens pasture systems, resulting in an increase, as a result, in the dry mass yield from the dosage of 121 m3 ha-1 and the reduction in fiber content in neutral and acid detergent. The increments in yield were observed in the second year, while improvement in bromatological quality was observed from the first application of LSM.

GISH-based comparative genomic analysis in Urochloa P. Beauv.

The genus Urochloa P. Beauv. [syn. Brachiaria (Trin.) Griseb.] comprises species of great economic relevance as forages. The genomic constitution for the allotetraploid species Urochloa brizantha (cv. Marandu) and Urochloa decumbens (cv. Basilisk) and the diploid Urochloa ruziziensis was previously proposed as BBB1B1, B1B1B2B2 and B2B2, respectively. Evidence indicates U. ruziziensis as the ancestral donor of genome B2 in U. decumbens allotetraploidy, but the origin of the genomes B and B1 is still unknown. There are diploid genotypes of U. brizantha and U. decumbens that may be potential ancestors of the tetraploids. The aim of this study was to determine the genomic constitution and relationships between genotypes of U. brizantha (2x and 4x), U. decumbens (2x and 4x) and U. ruziziensis (2x) via genomic in situ hybridization (GISH). Additionally, chromosome number and genome size were verified for the diploid genotypes. The diploids U. brizantha and U. decumbens presented 2n = 2x = 18 chromosomes and DNA content of 1.79 and 1.44pg, respectively. The GISH analysis revealed high homology between the diploids U. brizantha and U. decumbens, which suggests relatively short divergence time. The GISH using genomic probes from the diploid accessions on the tetraploid accessions' chromosomes presented similar patterns, highlighting the genome B1 present in both of the tetraploids. Based on GISH results, the genomic constitution was proposed for the diploid genotypes of U. brizantha (B1B1) and U. decumbens (B1'B1') and both were pointed as donors of genome B1 (or B1'), present in the allotetraploid genotypes.

Supplementation levels for pre-weaning grazing beef heifers during the rainy-dry transition season

This study was undertaken to examine production performance, pasture and milk intakes and nutrient digestibility in heifers supplemented with different levels of energy in a creep-feeding system on Urochloa decumbens Stapf pastures during the rainy-dry transition season. Fifty-one heifers with predominant Nellore genetic composition (average initial age and weight: 120 days and 133.4 ± 3.12 kg) and their respective dams were used in an evaluation of production performance, intake, and diet digestibility. Four treatments were evaluated, namely, MM - mineral mixture; T250 - 0.250 kg of supplement per head per day; T500 - 0.500 kg of supplement per head per day; and T750 - 0.750 kg of supplement per head per day, totaling three groups with 13 animals each and one group with 12 animals. All supplements were balanced to provide 100 g of CP animal-1 day-1. LIPE® and chromium oxide were used as external markers to estimate fecal excretion and individual DM and supplement intakes, respectively, whereas iNDF was used as an internal marker for pasture DM intake. Production performance did not differ between the supplemented and unsupplemented animals. Total DM intake and pasture DM intake were higher in the group supplemented with concentrate, and the same was observed for the digestibility of DM and NDFap consumed by the animals. The average cow milk yield was 5.33 kg/day, with average protein and fat contents of 3.51% and 4.85%, respectively. In conclusion, compared to the use of mineral mixture only, multiple supplementation at different levels containing 100 g of CP does not significantly improve performance in lactating beef heifers grazing on Urochloa decumbens Stapf and supplemented during the rainy-dry transition season.