Goat urine wastewater that is disposed of without being processed will certainly cause environmental pollution. Therefore it is necessary to process the livestock waste. Processing goat urine into fertilizer needs to be done by converting ammonia to nitrate, or what is called the nitrification process. Nitrification takes place in two stages of oxidation, the first is the oxidation of ammonium to nitrite, and the second is the oxidation of nitrite to nitrate with the help of oxygen. The materials used in this study were (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, K<sub>2</sub>HPO<sub>4</sub>, NaHCO<sub>3</sub>, Na<sub>2</sub>CO<sub>3</sub>, MgSO<sub>4</sub>.7H<sub>2</sub>O, FeSO<sub>4</sub>.7H<sub>2</sub>O, CaCl<sub>2</sub>.2H<sub>2</sub>O, ZnSO<sub>4</sub>, NaCl, H<sub>2</sub>SO<sub>4</sub>, NaNO<sub>2</sub>, KNO<sub>3</sub>, NH<sub>4</sub>Cl, Na<sub>2</sub>S<sub>2</sub>O<sub>3</sub> (solution distilled water, filter paper, Rochell salts, Nessler reagent, N- (1-naphthyl) -ethylenediamine dihydrochloride (NED dihydrochloride) solution, sulfanilamide solution, sulfuric acid brucine solution, NPK fertilizer, glucose, urea fertilizer, TSP fertilizer, KCl fertilizer, cornflour, tofu dregs. Goat livestock waste, namely goat urine, was taken from one of the farms in North Denpasar, and sediment sampling was carried out in the Badung river next to the goat farm. According to the result, the best medium for ammonia oxidizers pellets was Ammonia Oxidizer Media II (media for pellet b) which was built of 10 grams of NPK fertilizer, 10 grams of glucose, and 5 grams of NH<sub>4</sub>Cl powder. Furthermore, the sample performed the highest nitrite removal with a pellet dose of 20% (w/v) (pellet b) and the measurement time at 18 hours, with an average nitrite concentration of three repetitions 2.3992 mg/L.