Objectives. The urinary tract infections (UTIs) is the most common type of infection, after respiratory tract infections, being more common in women, approximately 50-60% of whom have at least one episode of UTI during their lifetime. Other risk factors are old age, diabetes, kidney stones, urinary tract obstructions, and procedures such as urinary catheterization. Uropathogenic strains of E. coli are most commonly implicated in the etiopathogenesis of UTI, other pathogens being represented by Proteus mirabilis, Staphylococcus saprophyticus, Staphylococcus epidermidis, Klebsiella pneumoniae, group B streptococci (involved, for example, in UTI in patients with diabetes mellitus) and Pseudomonas aeruginosa (found in patients who underwent urinary catheterization). The study aimed to test the sensitivity at antibiotics of bacteria isolated from urine samples of patients with urinary tract infections from a medical analysis laboratory, in the period 2022-2023. Methods. The urine samples obtained were initially analyzed macroscopically, from the point of view of turbidity and chromatic aspect. Later, they were centrifuged and examined under an optical microscope to assess the cellularity. Sectors were sown from the urine samples on CLED, CPS and bile-esculin culture media. After being seeded, the plates were introduced to the thermostat, where they were left for 24 hours at 36-37 °C. The antibiotic susceptibility of the bacterial strains was tested by means of the diffusimetric method, using special Muller-Hinton media. Results. The most common pathogen isolated in urine cultures was E. coli 83%, followed by Klebsiella pneumoniae (7%) and Enterococcus spp. (4%), 3 strains of Proteus mirabilis, 2 of Klebsiella oxytoca and 1 of Proteus vulgaris were also isolated. Regarding the susceptibility at the tested antibiotics, it has been obtained the following results: to AmoxicilinClavulanic Acid-77% Gentamicine-96%, Nitrofurantoin-88%, Trimethoprime/Sulfamethoxazole- 79% Cefazolin-71%, Cefuroxime-81% Levofloxacine-92%, Ciprofloxacine-91%, Norfloxacine-83%, Fosfomycin-99%. Conclusions. The isolated strains showed more than 90% sensitivity to Gentamicine, Levofloxacine and Ciprofloxacine. Also, an increased sensitivity (over 80%) was recorded to Cefuroxime and Nitrofurantoin.