You have accessJournal of UrologyKidney Cancer: Basic Research & Pathophysiology II1 Apr 2016MP85-19 URINARY PEPTIDOME AND PROTEOME ALTERATIONS RELATED TO TUMOR PROGRESSION AND INVASION IN RCC Clizia Chinello, Marco Grasso, Marta Cazzaniga, Gabriele De Sio, Angelica Grasso, Bernardo Rocco, Andrew Smith, Italo Zoppis, Giancarlo Mauri, and Fulvio Magni Clizia ChinelloClizia Chinello More articles by this author , Marco GrassoMarco Grasso More articles by this author , Marta CazzanigaMarta Cazzaniga More articles by this author , Gabriele De SioGabriele De Sio More articles by this author , Angelica GrassoAngelica Grasso More articles by this author , Bernardo RoccoBernardo Rocco More articles by this author , Andrew SmithAndrew Smith More articles by this author , Italo ZoppisItalo Zoppis More articles by this author , Giancarlo MauriGiancarlo Mauri More articles by this author , and Fulvio MagniFulvio Magni More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.2285AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Up to now, for renal cell carcinoma (RCC), tumor size and growth rate are the most used prognostic factors at diagnosis likely being related with potential progressive neoplasms. Moreover, the degree of the free tumor extension into renal vessel seems to contribute to disease-free survival and to influence prognosis. Thus, firstly, in order to highlight urinary peptidome signatures that might reflect kidney cancer progression, a systematic investigation by MALDI profiling was carried out identifying endogenous peptides that show a correlation between their abundance in urine and pT, tumor size and grade. Secondly, in order to explore proteome changes reflecting the local tumor infiltration through vena cava, a label-free nLC MS/MS was performed in trypsinized urine from subjects affected by RCC at different level of invasion (vascular endothelium/vein/ thrombosis). METHODS Peptidomic evaluation of tumor progression was performed in 117 RCC urine samples, through the application of C8 functionalized magnetic beads purification followed by MALDI-TOF and statistical analysis for group comparisons and correlations. nLC-ESI-MS/MS was used for peptide identification. Urine proteome investigation correlated to different renal vein invasion extent was carried out by label-free nLC MS/MS strategy using FASP digested pools of 3 groups. Statistical evaluation was obtained by Progenesis QI for Proteomics. RESULTS 15 endogenous peptides showed a statistically significant correlation between their urinary concentration and tumor size (only 3 negatively), 26 with pT (only 6 negatively), and only 5 with grade (only 1 positively). Most of them were differentially represented in urine of RCC patients compared to controls and for some of them varied according to pT or stage. Identity of several of them are likely to confirm their possible role in tumor progression. Proteomic labelfree approach regarding tumor invasion allowed to select 671 protein IDs significantly altered in at least one of the 3 group comparisons (fold change=2). 12 of them showed a progressive increase from the endothelium wall infiltration to thrombosis CONCLUSIONS These methodological approaches applied to urine may provide useful keys to highlight alterations triggered by RCC aggressiveness or vascular neoplastic infiltration from endothelial layer to vein ostium obstruction, better understand tumor protein handling aspects, and describe such a dynamic system as growing cancer cells are. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e1105 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Clizia Chinello More articles by this author Marco Grasso More articles by this author Marta Cazzaniga More articles by this author Gabriele De Sio More articles by this author Angelica Grasso More articles by this author Bernardo Rocco More articles by this author Andrew Smith More articles by this author Italo Zoppis More articles by this author Giancarlo Mauri More articles by this author Fulvio Magni More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...