Urinary Lactate Dehydrogenase Research Articles

Fifteen Years of Experience with Urinary Diversion in Myelomeningocele Patients

No AccessJournal of Urology1 Feb 1969Fifteen Years of Experience with Urinary Diversion in Myelomeningocele Patients Winston K. Mebust, John D. Foret, and William L. Yale Winston K. MebustWinston K. Mebust More articles by this author , John D. ForetJohn D. Foret More articles by this author , and William L. YaleWilliam L. Yale More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)62304-8AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail © 1969 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByWilliams M (2018) Ileal Conduits—Are They a Poor Last Resort?Journal of Urology, VOL. 194, NO. 5, (1190-1191), Online publication date: 1-Nov-2015.PÉREZ L, WILBANKS J, JOSEPH D and OAKES W (2018) UROLOGICAL OUTCOME OF PATIENTS WITH CERVICAL AND UPPER THORACIC MYELOMENINGOCELEJournal of Urology, VOL. 164, NO. 3 Part 2, (962-964), Online publication date: 1-Sep-2000.KURZROCK E and POLSE S (2018) RENAL DETERIORATION IN MYELODYSPLASTIC CHILDREN: URODYNAMIC EVALUATION AND CLINICAL CORRELATESJournal of Urology, VOL. 159, NO. 5, (1657-1661), Online publication date: 1-May-1998.Kiker J, Woodside J, Reed W, Borden T and Woodside M (2018) Urinary Lactic Dehydrogenase and Serum C-Reactive Protein as Means of Localizing the Site of Urinary Tract Infection in Patients with Ileal ConduitsJournal of Urology, VOL. 128, NO. 4, (749-751), Online publication date: 1-Oct-1982.Fair W (2018) Editorial CommentJournal of Urology, VOL. 128, NO. 4, (751-751), Online publication date: 1-Oct-1982.Brendler C and Stephenson T (2018) Urinary Diversion and Undiversion in ChildrenJournal of Urology, VOL. 125, NO. 4, (457-462), Online publication date: 1-Apr-1981.Schoenberg H, Shah J, Kyker J and Gregory J (2018) Changing Attitudes Toward Urinary Dysfunction in MyelodysplasiaJournal of Urology, VOL. 117, NO. 4, (501-504), Online publication date: 1-Apr-1977. (2018) CommentJournal of Urology, VOL. 117, NO. 4, (504-504), Online publication date: 1-Apr-1977.Cass A (2018) Urinary Tract Complications in Myelomeningocele PatientsJournal of Urology, VOL. 115, NO. 1, (102-104), Online publication date: 1-Jan-1976.Shapiro S, Lebowitz R and Colodny A (2018) Fate of 90 Children with Ileal Conduit Urinary Diversion a Decade Later: Analysis of Complications, Pyelography, Renal Function and BacteriologyJournal of Urology, VOL. 114, NO. 2, (289-295), Online publication date: 1-Aug-1975.Anderson R, Hill M, Chapman W and Shurtleff D (2018) Results of Social Versus Medical Ileal Conduit Diversion in patients with MyelodysplasiaJournal of Urology, VOL. 112, NO. 5, (587-590), Online publication date: 1-Nov-1974.Richie J (2018) Intestinal Loop Urinary Diversion in ChildrenJournal of Urology, VOL. 111, NO. 5, (687-689), Online publication date: 1-May-1974.Smith E (2018) Urinary Prognosis in Spina BifidaJournal of Urology, VOL. 108, NO. 5, (815-817), Online publication date: 1-Nov-1972.Cass A and Geist R (2018) Results of Conservative and Surgical Management of the Neurogenic Bladder in 160 ChildrenJournal of Urology, VOL. 107, NO. 5, (865-868), Online publication date: 1-May-1972.Malek R, Burke E and Deweerd J (2018) Ileal Conduit Urinary Diversion in ChildrenJournal of Urology, VOL. 105, NO. 6, (892-900), Online publication date: 1-Jun-1971. Volume 101Issue 2February 1969Page: 177-182 Advertisement Copyright & Permissions© 1969 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Winston K. Mebust More articles by this author John D. Foret More articles by this author William L. Yale More articles by this author Expand All Advertisement PDF DownloadLoading ...

The activity of the urinary lactate dehydrogenase isoenzymes in acute glomerulonephritis in childhood.

The activity of the urinary lactate dehydrogenase isoenzymes in acute glomerulonephritis in childhood.

Renal infarction. Elevation of serum and urinary lactic dehydrogenase (LDH).

Although renal infarction is not rare, clinical diagnosis is difficult. In one series of 205 patients found at autopsy to have a renal infarction, only two cases were diagnosed during life.1Recently, we have observed two patients in whom striking elevation of serum lactic dehydrogenase (LDH) was a clue to the diagnosis of renal infarction; in one of these patients, urinary LDH also was markedly increased. The association of increased serum and urine LDH with renal infarction has been described in detail only once previously,2although it has been mentioned in passing in two other reports.3,4 The purpose of the present paper is to emphasize that renal infarction must be considered in the differential diagnosis of serum and urinary LDH elevations. Patient Summaries Patient. 1. —A 69-year-old Negro woman was admitted to St. Louis City Hospital on July 14, 1966, during a heat wave, prostrate and with

Some properties of lactate dehydrogenase found in human urine.

1. Urinary lactate dehydrogenase was concentrated and subjected to starchblock electrophoresis. The isoenzyme pattern obtained was shown to be similar to that of the kidney cortex and medulla but different from that of the bladder and kidney pelvis. 2. The values for the relative activity and Michaelis constant of urinary lactate dehydrogenase were similar to those for kidney cortex and medulla but significantly different from those obtained for bladder and kidney pelvis. 3. The molecular weight of urinary lactate dehydrogenase was estimated by thin-layer chromatogtaphy on Sephadex G-200. The values obtained for several samples of urine ranged from 129 000 to 155 000 and were very close to that of the crystalline rabbit-muscle enzyme (140 000). 4. The question of the possible origin of urinary lactate dehydrogenase is discussed and the conclusion drawn that the kidney and not the plasma is the most likely source.

Significance of Total Urinary Lactic Dehydrogenase Activity in Urinary Tract Diseases

Significance of Total Urinary Lactic Dehydrogenase Activity in Urinary Tract Diseases

The Clinical Significance of Increased Activity of Urinary Lactic Dehydrogenase

The Clinical Significance of Increased Activity of Urinary Lactic Dehydrogenase

Urinary Lactic Dehydrogenase: A Report Based on 250 Hospitalized Patients

Urinary Lactic Dehydrogenase: A Report Based on 250 Hospitalized Patients

Urinary Lactic Acid Dehydrogenase Activity in the Diagnosis of Urologic Neoplasms

Urinary Lactic Acid Dehydrogenase Activity in the Diagnosis of Urologic Neoplasms

Automated assay of lactate dehydrogenase in urine

As part of a program under way in our laboratory to assess the significance of certain enzyme activities in urine as a diagnostic aid in urinary tract disease, we were prompted to seek a rapid, convenient method for determination of urinary lactate dehydrogenase (LDH). The procedure to be described is a modification of an automated enzymic procedure developed by us for determination of l-lactic acid in body fluids and tissues (1) utilizing the AutoAnalyzer. 3 3 Technicon Instruments Corporation, Chauncey, New York. The oxidation of l-lactate by NAD + catalyzed by lactate dehydrogenase is coupled, via diaphorase, to the reduction of a tetrazolium dye, and the absorbance is measured at 500 mμ. Under the conditions to be described, the reaction rate is strictly proportional to the enzyme activity, and the method is convenient also for determination of the enzyme in body fluids and tissues. The method is similar in principle but differs in detail from a manual procedure described by Nachlas et al. (2) and an automated method published recently by Hicks and Updike (3) while this work was in progress.

Urinary Enzymes and Their Diagnostic Value in Human Cancer

Most of the urinary enzymes contribute information on the presence and location of the neoplasm. The normal excretion of the urinary amylases, phosphatases, proteases, catalases, esterases, and other enzymes are altered both quantitatively and qualitatively in human cancer, especially in cancer of the urinary tract, giving clues to both the nature and location of the pathological growth. Urinary enzymes present in the urine have various sources. They may come from the surrounding normal or malignant tissues of the urinary tract. They may exist in the cellular components of the urine or in genital system. The enzymes may also exist in the secretion of macromolecules which enter urinary tract through passive diffusion. The simultaneous measurement of both the urinary lactic dehydrogenase and alkaline phosphatase activities provide a diagnostic pattern to detect and localize carcinomas of the urinary tract. Most of the serum and urinary enzymes, which have been intensively investigated, have proved to have some diagnostic value for cancer, although so far no single enzyme has proved to be 100% reliable. However, there are hopes that when more data become available on a variety of serum and urinary enzymes and isozymes in cancer patients and when rapid and accurate assay techniques are developed, enzyme and isozyme patterns of the body fluids will provide the clinician with reliable and specific means for the early diagnosis of human cancer.

Urinary Lactate Dehydrogenase Activity

(1) A method for measurering the total activity of urinary lactate dehydrogenase (urinary LDH) is presented. The optima of pH, substrate and Coenzyme for this enzyme were determined by measuring the decrease in optical density at 366 nm of NADH formed by reduction of pyruvate to lactate. By application of this method no urinary inhibitor of LDH was detected. (2) Urinary LDH activities were investigated in patients suffering from some severe renal diseases, including unilateral renal ischaemia. Simultaneously the urinary LDH-isozyme pattern was determined by means of zone electrophoresis in agar gel. (3) The value of this supplement to the measurement of the total enzyme activity is pointed out. Thus unilateral renal ischaemia may be associated with the relative predominance of LDH-isozyme five in the urine from the affected kidney.

URINARY ALKALINE PHOSPHATASE AND LDH ACTIVITIES IN THE DIFFERENTIAL DIAGNOSIS OF RENAL DISEASE.

Excerpt Urinary alkaline phosphatase and lactic dehydrogenase (LDH) activity measurements provide specific and sensitive tools for the detection and diagnosis of urologic disease. Both the urinary ...

Confirmation of the Source of Elevated Urinary Lactic Dehydrogenase in Patients with Renal Tumor

Confirmation of the Source of Elevated Urinary Lactic Dehydrogenase in Patients with Renal Tumor

Urinary Lactic Dehydrogenase Activity

RECENTLYA METHOD for the accurate measurement of the lactic dehydrogenase (LDH) activity of human urine has been developed.¹By use of this method it has been shown that the urinary LDH activity is elevated in patients with carcinoma of the kidney and bladder and that it can be employed for the detection and diagnosis of these tumors.^2,3 Measurement of urinary LDH activity in patients with other types of renal disease has not previously been performed. In the present study the urinary LDH activity has been measured in a large series of patients with renal disease, and in patients with benign essential hypertension, with the object of evaluating its diagnostic potential in these diseases. The results show that urinary LDH activity is elevated in most patients with significant renal disease, and that elevations of activity correlate well with the histological and clinical activity of the disease. Moreover, the

Diagnostic Significance of Urinary Lactic Acid Dehydrogenase

Diagnostic Significance of Urinary Lactic Acid Dehydrogenase

Urinary Lactic Dehydrogenase Activity

Lactic acid dehydrogenase (LDH) and one or more substances that inhibit its activity are constantly present in human urine. Dialysis removes the inhibitor or inhibitors and makes it possible to define the normal range of LDH activities (550 to 2,050 units per 8-hr volume of urine determined at 25 C [77 F]). Small but significant elevations may therefore be detected. Simultaneous determinations of LDH activity in urine and serum, of blood urea nitrogen, of total urinary protein, and of other urinary constituents were carried out on 50 patients. Increased LDH activities in the serum were not accompanied by commensurate increases in the urine. LDH activities in the urine had no detectable relation to the concentrations of other urinary constituents and were not affected by the presence of hematuria. High LDH activities in the urine appeared to be related only to disease of the urinary tract, particularly renal and vesical carcinoma.

URINARY LACTIC DEHYDROGENASE IN RENAL DISEASE

URINARY LACTIC DEHYDROGENASE IN RENAL DISEASE