Urinary Excretion Of Androsterone Research Articles

Gas chromatography-mass spectrometry based metabolomics of urine steroids in women with different phenotypes of polycystic ovary syndrome

This article presents an analysis of the metabolism of androgens, glucocorticoid hormones, and progestins obtained by gas chromatography-mass spectrometry in 56 women with obesity and hyperandrogenia in comparison with 23 women with alimentary-constitutional obesity and 25 healthy women. An increase in urinary excretion of androsterone, 16-oxo-androstenediol (16-oxo-dA2), and α-tetrahydrometabolites of cortisone and corticosterone has been established in 25 women with polycystic-altered ovaries according to ultrasound, with urinary excretion of dehydroepiandrosterone (DHEA) and its metabolites (dA2-17β, 16β-DHEA, 16-oxo-dA2, and androstentriol), α- and β-metabolites of glucocorticoids and androstenedione, and 5-en-pregnans increased in 31 women without signs of polycystic ovaries according to ultrasound. A decrease in the tetrahydrocortisol/tetrahydrocortisone ratio was found in women with polycystic ovaries, indicating a decrease in the activity of 11β-hydroxysteroid dehydrogenase type 1. Evidence for the increased 5α-reductase activity was obtained in women with different phenotypes of polycystic ovary syndrome.

Effect of regular training on the anthropometric parameters and urine steroids in childhood.

The changes in the anthropometric data and urine steroid metabolites caused by regular training in children in two age groups (11 and 14 years old) were investigated. The skinfolds of older girls participating in regular athletic, swimming or soccer training were thinner compared with age-matched control groups (P < 0.01) and their body mass and constitution were lower (P < 0.05). In the other groups no significant differences were observable in the anthropometric parameters. The trained children in all groups had significantly higher exercise times on the cycle ergometer (P < 0.01, in young boys P < 0.05). The strength of their hands was lower in three trained groups: in younger boys (P < 0.05), in younger girls (P < 0.01) and in older girls (right hand P < 0.01, left hand P < 0.05). The urinary excretion of androsterone (P < 0.02), 11-ketopregnanetriol (P < 0.01) and pregnenetriol (P < 0.02) was decreased in the older trained girls; pregnenetriol was increased in younger boys (P < 0.05). Urinary excretion of cortisol metabolites was increased in trained boys [in younger boys: tetrahydrocorticosterone (P < 0.05) and 20 alpha-hydroxycortisol (P < 0.05); in older boys allotetrahydrocortisol (P < 0.02), cortisol (P < 0.05) and 20 alpha-hydroxycortisol (P < 0.05)]. There were no significant differences in the younger girls.(ABSTRACT TRUNCATED AT 250 WORDS)

Isotretinoin treatment alters steroid metabolism in women with acne

The effect of isotretinoin (Roaccutane) on serum steroids and urinary steroid metabolites was investigated in seven female patients receiving the drug for treatment of severe acne over a 16-week period. Serum concentrations of dehydroepiandrosterone sulphate (DHAS), androstenedione (A2), and free androgen index (FAI) were not significantly altered. There was a significant fall in testosterone during treatment and a significant reduction in the 24 h urinary excretion of androsterone, tetrahydrocortisone (THE) and tetrahydrocortisol (THF) from week 8 onwards and for aetiocholanolone and allo-THF from week 12 (P less than 0.05). Although pretreatment levels of urinary steroid metabolites were not abnormal, the ratios of the 5 alpha/5 beta metabolites (androsterone:aetiocholanolone and allo-THF:THF) were at the upper limit of the reference range and were lowered after treatment, suggesting that 5 alpha-reductase activity is sensitive to isotretinoin.

Urinary excretion of androsterone and etiocholanolone in obese women: Correlation with the cellularity of adipose tissue

Urinary androsterone and etiocholanolone and the number and the size of fat cells were measured in 43 non diabetic, non hypertensive, non dislipidemic obese women without clinical evidence of any endocrinopathy. Androsterone and the androsterone/etiocholanolone ratio were significantly ( P < 0.005) higher in the obese women. The androsterone/etiocholanolone ratio was significantly ( P < 0.01) correlated with the mean fat cell weight. These findings might be in keeping with the frequent cases of oligo-amenorrhea and of slight hypertricosis that can be frequently observed in obese women.

Correlation between rises of gonadotrophins and rises of sex steroids during clomiphene test in men.

Serum FSH, LH and testosterone and urinary excretion of androsterone, aetiocholanolone, dehydroepiandrosterone and total oestrogens were measured in normal and oligospermic men before and after clomiphene stimulation. Responses in all parameters were highly variably from one individual to another and this great variation formed the basis for investigating the correlations between responses. There was no correlation between increase of FSH and LH or increase of LH and steroids. Also there was no correlation between rises of FSH and rises of androgens. But a significant correlation existed between rises of FSH and rises of oestrogens. This confirms that one action of FSH is to stimulate production of testicular oestrogens which presumably takes place in the Sertoli cells.

Urinary excretion of steroid hormone metabolites in men with duodenal ulcer

Duodenal ulcer is more common and usually more severe in men than in women. One hypothesis put forward to explain this sex difference is that men undergo more stress than women. Therefore, the urinary excretion of androsterone (A), aetiocholanolone (E) and 17-hydroxycorticoids has been determined in 25 normal men, and 22 hospital controls and 35 patients with duodenal ulcer (D.U.) before and after surgery. The excretion of steroids was the same in the normal controls arid the preoperative controls and D.U. patients. After surgery the controls showed a significant decrease in E excretion, and the D.U. patients had a decrease in excretion of E large enough to raise the A/E ratio. The results are not consistent with the view that endogenous androgenic or adrenocortical steroids maintain D.U.

Urinary excretion of metabolites of steroid hormones by men with cancer of the stomach

The urinary excretion of androsterone (A), aetiocholanolone (E) and 17-hydroxycorticosteroids (17-OHCS) and the A/E ratio have been measured in 22 male hospital controls, 14 men with gastric ulcer, 22 men with cancer of the stomach and 14 men with cancer of the rectum before and after surgery, and also in 25 normal men. There were no significant differences in the excretion patterns of normal men, hospital controls and patients with gastric ulcer. The stomach cancer patients excreted less A ( P < 0.004) and E ( P < 0.02) and had a lower A/E ratio ( P < 0.05) than the hospital controls before surgery. After surgery the excretion pattern was not significantly different from the postoperative values for the hospital controls. The rectum cancer patients excreted less A ( P < 0.004) and had a lower A/E ratio ( P < 0.01) than hospital controls before surgery, but after surgery excreted less A ( P < 0.001) and E ( P < 0.02) and had a lower A/E ratio ( P < 0.01) than the postoperative hospital controls.

Urinary and plasma androgens and their relation to familial risk of breast cancer

The urinary excretion of androsterone, aetiocholanolone and dehydroepiandrosterone has been measured in 87 daughters and 39 sisters of women with breast cancer. Also 175 women who had no known family history of breast cancer were studied. Similarly, plasma dehydroepiandrosterone sulphate (DS) and androsterone sulphate (AS) were determined in 52 sisters and 55 daughters of women with breast cancer. There were 290 control women. The urinary excretion of aetiocholanolone was significantly lower (P < 0·05) in young sisters ( 30–40 years) compared to controls. Similarly, the age-adjusted geometric mean level of androsterone was lower (P < 0·05) in unaffected sisters compared to controls. No significant differences were found between daughters of breast cancer patients and controls for any of these urinary steroids. Neither was the general level of dehydroepiandrosterone different for any of the groups studied. The results for plasma DS and AS were similar to those for androsterone and aetiocholanolone. Thus the age-adjusted geometric mean level of DS and AS for sisters of breast cancer patients was significantly lower than that for controls (P < 0·025 and P < 0·005, respectively). There were no significant differences in the concentration of these steroids between daughters and controls. The main difference between unaffected sisters and controls appears to occur in the youngest age group studied ( 30–40 years). These results demonstrate an association between increased risk of breast cancer due to familial history and altered endocrine function.

Urinary excretion of androsterone throughout the menstrual cycle in the rhesus monkey (Macaca mulatta).

Urinary excretion of androsterone throughout the menstrual cycle in the rhesus monkey (Macaca mulatta).

Effect of resection of lung tumours on the steroid abnormalities in patients with lung cancer.

The urinary excretion of androsterone, aetiocholanolone, total 17-oxosteroids, and 17-hydroxycorticosteroids (17-OHCS) was measured in 40 patients with lung cancer three days before resection and again 10-15 days after resection of their lung tumours. There was a significant postoperative increase in the excretion of 17-OHCS but a significant decrease in the excretion of androsterone and aetiocholanolone, resulting in an increase of the preoperative abnormalities in steroid excretion in these patients. Since there was no change in steroid excretion towards normal after resection of the lung tumours, it seems that the steroid abnormalities found in lung cancer are not the effect of the presence of the lung tumours. As the excretions of 17-OHCS and 11-deoxy-17-oxosteroids change in opposite directions after resection, it is suggested that a dissociation of factors that control the excretion of these two groups of steroids takes place as a response to surgical stress in patients with lung cancer.

PROGNOSTIC SIGNIFICANCE OF A STEROID-DISCRIMINANT FUNCTION IN PATIENTS WITH INOPERABLE LUNG CANCER

A discriminant function based on the urinary excretion of androsterone (A), ætiocholanolone (E), the total 17-oxosteroids and the total 17-hydroxycorticosteroids (17-OHc.s.), which was used earlier to classify patients with lung cancer and controls, has been found to correlate very significantly with the period of survival from the date of diagnosis in fifty-six patients with inoperable lung cancer. Among the four variables in the discriminant function, androsterone showed the best correlation with the period of survival. The 17-OHc.s./A ratio but not the 17-OHc.s./E ratio was of as much prognostic value as the discriminant.

DISCRIMINANT FUNCTION BASED ON STEROID ABNORMALITIES IN PATIENTS WITH LUNG CANCER

The urinary excretion of androsterone. ætiocholanolone, dehydroepiandrosterone, total 17-oxosteroids, and total 17-hydroxy-corticosteroids (17-OHc.s.) was measured in two or three consecutive early-morning specimens of urine from one hundred and forty-two lung-cancer patients, fifty-two inpatient controls, and a hundred healthy controls. Patients with lung cancer have low androsterone/ætiocholanolone ratios and high 17-OHc.s./ androsterone ratios compared with controls. When the steroid values are combined in a discriminant function the error of misclassification is less than 10%. The low androsterone/ætiocholanolone ratio does not change after the surgical removal of the tumour. The discriminant function is lower in patients with inoperable tumours than in patients with operable tumours and may, therefore, be of diagnostic or prognostic value.

Quantitative analysis of urinary 11-deoxy-17-ketosteroids and pregnanediol by gas-liquid chromatography.

ABSTRACT A quantitative gas chromatographic method for the routine determination of urinary 11-deoxy-17-ketosteroids as their trimethylsilyl ethers, and of pregnanediol as the diacetate on a single column with 1 % SE-30 and 2% XE-60 as stationary phase, has been described. After hydrolysis, the 17-ketosteroids are purified by alumina column chromatography, converted into the trimethylsilyl ether derivatives, and quantitated by gas-liquid chromatography. Two procedures are described for the analysis of pregnanediol, using alumina column chromatography or Girard T separation as preliminary purification. Final purification is achieved by thin-layer chromatography (TLC) of the pregnanediol diacetate, followed by gas-liquid chromatography (GLC). The method has been applied to study the urinary excretion of androsterone, etiocholanolone and dehydroepiandrosterone in normal subjects before and after administration of corticotropin, Metopirone and dexamethasone. ACTH administration caused a marked increase in the...