Rapid urbanization has caused various urban environmental challenges, including the emergence of urban heat island and air pollution. Urban wind flow is a crucial factor shaping the urban environment, which needs effective prediction and design. Computational fluid dynamics is commonly used for prediction and design of urban wind environment. However, high-accuracy simulation of wind flow, especially considering the layouts of full-scale and complex buildings, requires intensive computational resources. Typically, simulations simplify the surrounding building layouts (e.g., building layers) of target areas, potentially impacting simulation accuracy. It is imperative to determine appropriate building layers around different-scale target areas for effective wind flow simulation. This work explored the simulated wind flow characteristics of multi-scale (local, micro, and building-scale) target areas with different building layers defined as H, L, H/L, and L/H (H and L representing the vertical and horizontal maximum sizes of target area). The recommended building layers for local-scale, micro-scale, and building-scale target areas were at least 3L, 4H, and 5H, respectively, ensuing the relative errors between simulated and measured wind velocities remained below 20 %. This method largely reduced the calculation time by 73 % compared with full-scale simulation. This work can provide guidelines for fast prediction and design of urban wind environment, further promoting the sustainable development of urban cities.