In the production of quality plant, it is important to consider the growing substrate since it provides plants with very important physical and chemical characteristics of the soil. Many organic materials of vegetal or animal origin have been used in mixtures of substrates for plant production. This study evaluated the development, quality and physiological components of Conilon coffee plants as a function of substrates containing organic materials of different origins. The experiment was carried out in a greenhouse, in a randomized block design with five treatments: T-Control treatment with 100% of soil and the other treatments with 85% of soil and 15% of organic matter derived from urban waste compost, mature cattle manure, dairy residue, and tannery sludge. Development, quality and physiological characteristics of plants were evaluated. All sources of organic matter favored the development of the Conilon coffee plants in relation to the plant receiving no organic matter in the substrate. Dehydrated tanning sludge in the proportion of 15% provided inferior plant performance compared with treatments with the other organic sources and a greater production of flavonoids. The treatments T-Compound, T-Dairy, and T-Manure provided better results than the other treatments for most of the evaluated characteristics, and can be recommended as components of substrates in the production of Conilon coffee plants. Key words: Coffea canephora, alternate substrate, sustainability, Dickson quality index, multiplex.