Understanding the relationship between urban morphology and land surface temperature (LST) is essential for mitigating urban heat island (UHI). This study investigates the spatiotemporal heterogeneity of the relationship between urban morphology and LST across 5 experiment sites located in different temperature zones of China at a block scale. Normalized difference vegetation index (NDVI), building density (BD), floor area ratio (FAR), average building height (ABH) and open space ratio (OSR) are adopted to indicate the physical urban morphology. The results show positive relationship between BD and LST, and negative relations between LST and NDVI, ABH and OSR from a global perspective. The relations of FAR to LST are mixed. However, variable relationship between urban morphological indicators (UMIs) and LST are significantly observed at a block scale. 12 scenes that generate local relations of UMIs and LST differing from that of the surroundings are identified. Typical strategies for land development, vegetation phenology, large natural elements and human activities may be the most important causative factors to the heterogenous relationship between urban morphology and LST. Findings derived in the study would promote studies on mechanisms of the effects of urban morphology on LST and contribute to practices of UHI mitigation.