INTRODUCTION: According to the most recent data from the U.S. Centers for Disease Control and Prevention, Latinos have higher rates of human papillomavirus (HPV) associated cancers when compared to the general population. Individuals who lack knowledge about HPV and its association with cancer may not understand the importance of preventing infection and may have less access to vaccination. This study aims to assess the knowledge regarding HPV transmission and perception of HPV vaccination in a Latino health clinic. METHODS: In this cross-sectional survey, a convenience sample of Latino participants in an urban free health clinic completed a survey in Spanish evaluating demographics and knowledge regarding the human papillomavirus disease, testing and vaccination using a previously validated questionnaire. Demographic characteristics were compared using chi-square and Fisher exact tests for HPV knowledge outcomes. RESULTS: Responses were obtained from 48 participants, 62.5% female and 37.5% male with mean age 37. 56% of the population studied had heard of HPV with only 27.8% of the males surveyed and only 33% of the population with a middle school education or less stating they had heard of HPV. A large percentage (81.5%) believed HPV could be cured with antibiotics or that if a woman tested positive for HPV she would definitely have a diagnosis of cervical cancer (45.5%). CONCLUSION: A large percentage of population surveyed had little knowledge about HPV testing and vaccination. Follow up research is needed to evaluate best methods/practice for dissemination of information and ensuring access to the HPV vaccine.