Urbanization is a key factor driving water quality and biological communities in freshwater systems. While urbanization impacts on water resources were well-studied in urban areas, few studies have examined the growing impacts in non-urban areas. Here we investigate the spatiotemporal trends of urbanization using high-resolution impervious surface data of both urban and non-urban areas across Germany, and evaluate the urbanization impacts on changes of run-off, nutrient emissions, and macroinvertebrate communities. The emission changes were modelled using the MONERIS-PCRaster tool, while the macroinvertebrate community responses to urbanization were determined using Generalized Least Square models. We find that urbanization expanded by 3.2% from 2006 to 2015 nationwide, and primarily occurred in less-populated regions. The non-urban areas contributed 19.3–19.6% of the imperviousness expansion nationwide, which were equivalent to the ‘unaccounted’ increases of 15.5–15.9% of run-off, 10.6–11.0% of total nitrogen, and 12.5–12.9% of total phosphorus when only emissions from urban areas were modelled. Incorporating the emissions from both urban and non-urban areas revealed significant impacts of urbanization on macroinvertebrate community composition changes, with increases in more pollution-resistant and non-native species (particularly in large rivers), and reductions of more sensitive taxa. The community responses were jointly determined by local catchment characteristics and urbanization stressors. Our results suggest neglected effects of non-urban impervious surfaces and the importance of using impervious surface data instead of urban land classes to better represent urbanization processes and impacts in long-term planning and management of freshwater systems.
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