The impact of urban form on carbon emissions has become a crucial issue for sustainable socioeconomic development and the advancement of low-carbon cities. Transportation is a significant source of urban carbon emissions, highlighting the need for comprehensive research to aid China in achieving its carbon peak and neutrality goals. Currently, there is a lack of quantitative studies exploring the effects of urban form on transportation-related carbon emissions. This paper seeks to quantify the effect of urban form on the spatial and temporal patterns of transportation carbon emissions, utilizing panel data from 27 cities in the Yangtze River Delta (YRD) region of China, covering the years 2000 to 2020. First, CO2 emissions from transportation are estimated following IPCC guidelines, with Moran’s I utilized to analyze spatial autocorrelation. Next, urban form indicators are quantified based on landscape ecology theory. Finally, econometric models are employed for regression analysis of the panel data. The findings reveal that urban complexity, compactness, and expansion influence transportation carbon emissions to varying degrees, with urban expansion and complexity associated with increased emissions, while compactness contributes to their reduction. This study offers theoretical support and a scientific basis for low-carbon urban spatial planning and development, underscoring the importance of urban form in emissions reduction strategies.