Upstream Locations Research Articles

Study on oil-cooler location for a pusher type turbo prop aircraft using numerical simulation

PurposePusher configured turbo-prop aircraft receive inadequate ram air cooling due to the lack of propeller slipstream, particularly during ground operations. However, flow entrainment can be exploited to a greater extent by placing the oil-cooler duct close to downstream of the propeller at a suitable radial location. But this method has a detrimental effect on the propeller thrust. The purpose of this paper is to discuss the results of numerical simulations carried out to simulate the performance of the propeller with and without oil cooler.Design/methodology/approachIn this paper, three-dimensional (3D) numerical simulations are carried out to simulate the propeller in a rotating domain using an unstructured grid. A computational fluid dynamics solver is put forward to analyze the effect of thrust loss by solving 3D Navier-Stokes equations using a second-order upwind finite-volume scheme. In this study, the impact of thrust loss incurred in the propeller flow field with and without oil cooler duct for three different locations at various rotational speeds is carried out to assess the propeller performance and to identify the optimum position to get a sufficient mass flow rate.FindingsThe findings from this study are simulated thrust values of an uninstalled five-bladed propeller of light transport aircraft (LTA) match well with original equipment manufacturer propeller thrust data. The tip speed velocities simulated for different operating conditions are in good agreement with the theoretical calculations. The influence of oil-cooler effect on the propeller flow field is less in low velocity to high-velocity operating condition due to flow transition from laminar to turbulent. The presence of the oil cooler, which influences the thrust loss, is studied at propeller upstream and downstream locations in detail for 30%, 40% and 50% of propeller radius cases.Research limitations/implicationsSimulations with finer and structured hexa grids can be applied to this problem to get closer results and save solver time as future work.Practical implicationsThe recommended system is installed in the production standard aircraft of LTA. After installation oil cooler performance is better compared to the previous arrangement.Originality/valueResearch work about pusher aircraft is very limited. The problem addressed in this study is unique which resolves the major issue of pusher aircraft. This work highlights the difficulty involved in LTA engine oil cooling, and solution methodologies are also provided. Numerical simulation with oil-cooler assembly is a new area of research that gave the solution for this oil-cooling issue through various oil-cooler case studies.

Environmental DNA Monitoring of Biodiversity Hotspots in Danish Marine Waters

We investigated the use of eDNA metabarcoding for supplementing traditional diver-based monitoring of biodiversity of marine boulder reefs within the photic zone. The applied sampling design made it possible to evaluate the usefulness of eDNA monitoring as a supplement for traditional monitoring. Specifically, this study aimed to (1) assess the local influence of boulder reefs on biodiversity across the North Sea to Baltic Sea transition zone and (2) investigate the importance of environmental gradients for patterns in community structure. On samples collected during August 2020, we compared the composition and abundance of species associated with nine reefs, representing an environmental gradient of salinity (16–33 psu), water temperature (16–21°C) and water depth (6–29 m). At each reef site, water was sampled near the bottom just above the reef and on average 2.6 km upstream and downstream (location) and sequenced with metabarcoding using COI, 18S and 12S rDNA primers. eDNA identified 400 species, diver-based observations identified 184 with an overlap of 70 species (12%) and 81 genera (18%). While eDNA identified many infaunal species, it did not detect several macroalgal species which dominated in the diver-based observations. Multivariate analysis of eDNA and diver-based community structure both distinguished between reef communities, with a significant match between patterns observed by the two methods (r = 0.37, p = 0.02). Furthermore, the eDNA approach made it possible to identify significant differences in species composition between upstream, above-reef and downstream locations, suggesting that eDNA leaves a local footprint in benthic habitats. Patterns in both eDNA and diver-based species composition and richness were significantly related to geographical distance, salinity, water temperature and water depth. Despite of low detection of macroalgae, the eDNA sampling provided a substantial supplement to traditional diver-based monitoring of biodiversity around benthic hotspots in the Danish marine waters and therefore we recommend to add eDNA methods to conventional monitoring programs in the future.

Ecogenotoxicological studies for an early toxicity screening and monitoring in Epinephalus chlorostigma and Scamberomorus commerson

The study was planned to investigate DNA fragmentation in fish to screen aquatic toxicity and in Epinephalus chlorostigma and Scamberomorus commerson collected from Red sea near Jizan, Saudi Arabia from three locations “(Corniche North park: “16.92161, 42.54631; Jizan Port: 16.874, 42.54952” N and Jizan Economic City: 17.26589, 42.34738“ ”)“ were used as a case study for the application of comet assay. The study area of the Red Sea is polluted due to anthropogenic activities and the disposal of wastes from multiple sources. Comet and micronucleus assays were used to detect genotoxicity in these fish species harvested from three sites. The concentration of Pb, Cr, Zn, Mn, Cu, Cd, Sn, and Hg was higher in the water samples collected from the polluted site compared to the non-polluted site of the Red sea. Comet assay for S. commerson showed significant (p < 0.05) genetic damage about 44.33 ± 3.03% DNA in comet tail at site S1. It was subsequently reduced to 31.71 ± 3.52% and 22.11 ± 2.52% at sites S2 and S3. E. chlorostigma also showed significant DNA in comet tail as 17.34 ± 2.19%, 11.87 ± 3.01%, and 36.41 ± 3.98% at site S1-S3, respectively. Significant (p < 0.05) DNA damage was observed in the fishes procured from non-polluted locations and upstream locations. The micronucleus induction in E. chlorostigma was recorded as 23.20 ± 4.19 and 2.20 ± 0.58%, respectively, non-polluted and polluted sites. S. commerson exhibited significant differences between polluted and non-polluted sites (44.80 ± 3.73 and 8.20 ± 2.20‰) polluted and upstream (44.80 ± 3.73 and 20.60 ± 4.02‰), respectively. A significant difference was obtained between E. chlorostigma and S. commerson for nuclear abnormalities S. commerson showed higher frequencies for nuclear deformities than E. chlorostigma. S. commerson showed substantial micronucleus induction frequencies collected from an area of low pollution intensity (upstream). This study showed that E. clorostigma and S. commerson could be successfully used as a bioindicator to determine the health of the Red Sea through the most specific assays such as comet and micronucleus tests as an early warning and to devise the monitoring strategies to ensure a safe supply of fish for human consumption.

Intelligent polarization-sensitive holographic flow-cytometer: Towards specificity in classifying natural and microplastic fibers

Micron size fiber fragments (MFFs), both natural and synthetic, are ubiquitous in our life, especially in textile clothes, being necessary in modern society. In the Earth's aquatic ecosystem, microplastic fibers account for ~91% of microplastic pollution, thus deserving notable attention as one of the most alarming ecological problems. Accurate automatic identification of MFFs discharges in specific upstream locations is highly demanded. Computational microscopy based on Digital Holography (DH) and machine learning has been demonstrated to identify microplastics in respect to microalgae. However, DH is a non-specific optical tool, meaning it cannot distinguish different types of plastic materials. On the other hand, materials-specific assessments are pivotal to establish the environmental impact of different textile products and production processes. Spectroscopic assays can be employed to identify microplastics for their intrinsic specificity, although they are generally low-throughput and require large concentrations to enable effective measurements. Conversely, MFFs are usually finely dispersed within a water sample. Here we rely on a polarization-resolved holographic flow cytometer in a Lab-on-Chip (LoC) platform for analysing MFFs. We demonstrate that two important objectives can be achieved, i.e. adding material specificity through polarization analysis while operating in a microfluidic stream modality. Through a machine learning numerical pipeline, natural fibers (i.e. cotton and wool) can be clearly separated from synthetic microfilaments, namely PA6, PA6.6, PET, PP. Moreover, the proposed system can accurately distinguish between different polymers under investigation, thus fulfilling the specificity goal. We extract and select different features from amplitude, phase and birefringence maps retrieved from the digital holograms. These are shown to typify MFFs without the need for sample pre-treatment or large concentrations. The simplicity of the DH method for identifying MFFs in LoC-based flow cytometers could promote the use of polarization resolved field-portable analysis systems suitable for studying pollution caused by washing processes of synthetic textiles.

A computational study of curvature effect on pressure drop of gas-liquid two-phase flow through 90 degree elbow

The CFD analysis of air-water two-phase flows was performed in a 90o vertical to horizontal elbows made of PVC with four different inside diameters (25 mm, 50 mm, 75 mm, and 100 mm) with six different curvature radius to diameter ratios (R/D) 1, 2, 4, 6, 8, and 10. Pressure drops were investigated at various upstream and downstream locations using computational, experimental, empirical methods. The most effective method in investigating and determining total pressure drop of two-phase flow in pipes and bends is considered to be computational study. The CFD simulations were performed using ANSYSY 19.2 FLUENT and a mixture model. The studies were conducted under the following two-phase conditions: mass quality from 1-50% and mass flux from 350-1000 kg/m2s. The results show that the impact of these significant parameters are important and dramatic specially at high curvature radius, mass flux and mass quality. The results of the CFD study demonstrated a substantial loss in energy and pressure as the fluid exits the elbow section, and also a higher drop in pressure observed at higher air velocity. Also, higher pressure drops were obtained with increasing pipe diameter in one side, and with decreasing R/D on the other side. Finally, the current results were verified with the empirical and experimental studies and a good agreement were obtained.

Temporal trend of perfluorinated compounds in untreated wastewater and surface water in the middle part of the Danube River belonging to the northern part of Serbia

The occurrence and temporal variation of selected priority substances and contaminants of emerging concerns, i.e., eleven perfluorinated compounds (PFCs, belonging to perfluorosulphonates, perfluorocarboxylic acids, and perfluorinated sulphonamides) have been investigated in composite surface water samples of the Danube River at the upstream and downstream locations from the discharge point of wastewater. Among the analyzed compounds, six PFCs (PFOA, PFOS, PFHxA, PFNA, PFDA and PFUnA) were quantified. Overall, the detection frequency for most quantified compounds was very high (&gt;90 %), only PFDA and PFUnA were quantified with less frequency, 33 and 67 %, respectively. The highest quantified concentrations of PFOA and PFOS were 14.9 ng/L (average 12.1 ng/L) and 14.2 ng/L (average 6.11 ng/L), respectively. These ones together with PFHxA (average 10.0 ng/L) were quantified at the highest concentrations in comparison to the other investigated compounds. However, the determined levels of PFOS during investigated sampling period, for all samples analyzed, were always lower than the maximum allowable concentration set for inland river waters but always higher than the environmental quality standard threshold value-AA-EQS of 0.65 ng/L sets by the Directive of European Parliament. Moreover, the levels of PFOA were always several times lower than the set AA-EQS value.

Hydrochemical interfaces using water quality index in ascertaining integrity of river Ikpoba, South-South Nigeria

Investigation on the quality of river water resources, management and hydro-chemical interfaces is vital for monitoring and ascertaining of surface-water contaminants sources. River Ikpoba samples were gotten from various sampling points at a distance of 10 m apart along the stretch of the river during the dry season (November 2020) and wet or rainy season (July 2021). These samples physicochemical parameters were scrutinized using and the outcome was used to compute coefficient of variability, water quality index (WQI), multivariate test as well as descriptive and L-moment statistics. The result displays that WQI of 56.2% sampling locations is categorize as excellent water in both seasons. Besides, the WQI values gotten during the scrutiny displayed deterioration changes in water quality from up-stream location to down-stream location, which in turn, signifies that river water is of poor standard or quality. Beyond, the periodic values of WQI suggest that throughout wet season, the river water is more badly influenced than during dry season. Similarly, analyses using thrice variable probability distribution model, explicitly; generalized pareto distribution (GPA); generalized extreme value distribution (GEV), as well as generalized logistics distribution (GLO) with the idea of ascertaining the appropriate fit probability/ likelihood distribution model, predicted (forecasted) rainfall quartile magnitude (Qt) created on the GLO model vary between 83.55 mm at two years (2yrs) return period to 133.68 mm at 100 years return period. Equally, r2 which is the coefficient of determination for the experimental vs. predicted (forecasted) rainfall created on the appropriate fit model was witnessed to be 0.9999.

Microbiological Characteristics and Heavy Metal Pollution of Crude Oil Contaminated Water Bodies in Port Harcourt Metropolis, Nigeria

Crude oil exploration has been beneficial to our economy but detrimental to our environment with the artisanal refineries further compounding the challenge. This research was aimed at investigating the microbiology and heavy metal pollution of three crude oil polluted rivers in Rivers State, Nigeria and effects on living organisms inhabiting that environment. This study was carried out in three locations in South-South Nigeria (Eagle Island, Iwofe and Chokocho rivers). A total of 64 water samples (upstream and downstream points) were collected using appropriate containers and sterile polyethene bags for 6 crab samples for a period of three months. Water and crab samples were analyzed for heavy metals using Atomic Absorption Spectrophotometric method while microbiological analysis involved isolation and enumeration of microbial populations of the water and crab samples as well as characterization and identification of the isolates using standard methods Results showed Total Heterotrophic bacteria (THB) ranged from 6.0 × 106 cfu/ml to 9.0 × 108cfu/ml for the downstream locations and 1.7 × 106cfu/ml to 3.5 × 107cfu/ml for the upstream locations. Total Heterotrophic Fungi (THF) ranged from 2.0 × 104cfu/ml to 1.1 × 105cfu/ml for downstream locations and 0.1 × 104cfu/ml to 4.0 × 104cfu/ml for upstream locations, Hydrocarbon Utilizing Bacteria (HUB) ranged from 0.8 × 103cfu/ml to 4.0 × 103cfu/ml in downstream locations and 2.0 × 103cfu/ml to 7.4 × 103cfu/ml in upstream locations, Hydrocarbon Utilizing Fungi (HUF) ranged from 1.0 × 103cfu/ml to 6.0 × 103cfu/ml for downstream locations and 5.0 × 102cfu/ml to 8.0 × 103cfu/ml for upstream locations. The bacteria identified biochemically included Serratia sp., Enterobacter sp. and Salmonella sp. for the crab samples and Bacillus sp. was dominant in the water samples (Bacillus subtilis, Bacillus cereus, Bacillus carboniphilus). The heavy metals (Fe, Ni, Zn, Cd, Cu, Mn, Cr and Pb) were below the DPR permissible limit but are likely to increase since the activities leading to pollution are still ongoing. The crab as a filter feeder, had higher concentration of heavy metals and microbial population and the location with the highest crude oil pollution (14.5mg/l) had the lowest THB (6.0 × 106cfu/ml) as physicochemical parameters like the amount of Dissolved Oxygen had been altered.

Experimental and numerical studies of one-directional and bi-directional flow conditions across tube banks heat exchanger

Bi-directional flow condition imposes different fluid dynamics and temperature changes compared to that of the usual one-directional flow condition. Bi-directional flow can be found in applications like thermoacoustic systems that offer a green technology for at least two major applications: refrigeration and power production. The technology is appealing as an alternative to traditional systems as it offers the replacement for the use of harmful working media and exhausted resources with the use of inert gaseous with relatively fewer moving mechanisms. As the fluid dynamics and heat transfer of bi-directional flow in a thermoacoustic working environment is less known, it is difficult to estimate losses and gain, especially during the design stage. This paper reveals the differences to be expected in the behaviour of flow and heat transfer through experimental as well as Computational Fluid Dynamics (CFD) results of one-directional and bi-directional flow conditions. Two different drivers were used to create the two different flow conditions: a loudspeaker for the bi-directional flow and a centrifugal blower for the one-directional flow. Both conditions were monitored based on flow amplitude that is calibrated between the two drivers. Results of velocity, temperature and, vorticity are recorded for Reynolds number that ranges between 270 and 1700. Analyses are supplemented with data from validated two-dimensional computational fluid dynamics models that were solved using the Shear-Stress-Transport (SST) k-ω turbulence model with second-order accuracy for all equations. Interesting features of differences in temperature and velocity changes between the one-directional and the bi-directional flows are reported. The temperature and velocity at upstream and downstream locations of the tube banks heat exchanger are almost the same for bi-directional cases but are significantly different when a one-directional flow is flowing over the heated tubes. In addition, the interplay between natural and forced convections is seen to affect the results that were recorded for the two flow conditions. The presence of thermally developing and fully developed regions is also discussed. The results indicate that the heat transfer behaviour of bi-directional flow is not the same as in the one-directional flow and the future calculation for heat transfer for bi-directional flow conditions of thermoacoustic must be carefully done with consideration of changes of flow conditions between the one-directional and the bi-directional flow conditions so that error could be minimized in the evaluation of the system’s performance.

Scalar gradient and flame propagation statistics of a flame-resolved laboratory-scale turbulent stratified burner simulation

A bluff-body stabilised turbulent jet flame burning in a stratified mode of combustion for fuel-lean methane/air mixtures is investigated by a flame-resolved simulation. A tabulated chemistry approach based on premixed flamelet generated manifolds (PFGM) accounts for thermochemistry. The computations are performed for a grid-resolution of 100 µm, sufficient to resolve the thermal flame thickness. The look-up table is generated with a mixture-averaged diffusivity assumption and the preferential diffusion fluxes are included in the simulation, using an efficient method without increasing the dimensionality of the manifold. The predicted mean and RMS quantities are found to be in good agreement with the experiment. The investigation focuses on the comparison of the combustion behaviour of the upstream locations close to the inlet with weak mixture fraction gradients to the downstream locations away from the inlet with strong mixture fraction gradients. Statistical analysis of the diffusion terms within the flame revealed that the preferential diffusion remains significant near the inlet, in the recirculation zone over the bluff-body. The structure of the stratified flame in downstream locations is statistically analysed in terms of displacement speed and scalar gradients by comparing against the corresponding quantities in upstream locations with weak mixture fraction gradients. It is shown that the displacement speed is statistically different between the flames under weak or strong mixture fraction gradients due to the influence of molecular diffusion and reaction rate components. However, increased curvature effects and cross-dissipation component also play a role. Finally, scalar dissipation and cross-dissipation rates of the mixture fraction fluctuation and the reaction progress variable fluctuation within the flame-brush are investigated, and their closure approaches are discussed.

Monsoon dilutes the concurrence but increases the correlation of viruses and Pharmaceuticals and Personal Care Products (PPCPs) in the urban waters of Guwahati, India: The context of pandemic viruses

Concurrence of pharmaceuticals and personal care products (PPCPs), pathogenic viruses, metals and microbial pollution along with their seasonal variations in the water environment are overarching in the context of existing pandemic, especially for tropical countries. The present study focuses on the seasonal influence on the vulnerability of urban water in Guwahati, the largest city in North-eastern India, through examining the concurrence of seven PPCPs, five viruses, faecal bacteria and nine metals in surface waters during monsoon (Summer-July 2017) and pre-monsoon (Winter-March 2018). Surface water sampling was carried out at different locations of the Brahmaputra River, its tributary Bharalu River (an unlined urban drain), and Dipor Bill Lake (Ramsar-recognized wetland). Both PPCPs and viruses were at high concentrations (e.g. up to 970 ng L−1 caffeine, 2.5 × 103 copies mL−1 pepper mild mottle virus (PMMoV)) at the confluence points of urban drains and the river, while they were mostly undetectable at both upstream and downstream locations, implying strong self-purification ability of the river. All the analysed PPCPs and viruses were at much higher concentrations during pre-monsoon i.e., winter than during monsoon, implying heavy dilution and temperature effect during the monsoon. Overall, PPCPs and viruses were more correlated in monsoon but the risk quotient in the urban tributary was higher in pre-monsoon (e.g. 5061 in pre-monsoon and 1515 in monsoon for caffeine). PMMoV was found to be an excellent faecal pollution indicator due to its prevalence, detectability and specificity in all seasons. Overall, the seasonal fluctuations of the non-enveloped viruses monitored in this study is likely to be relevant for SARS-CoV-2. We contribute to address the literature scarcity pertaining to seasonal variations in the prevalence of viruses and their concurrences with contaminants of emerging concern.

A Tale of Two Deltas: Dam-Induced Hydro-Morphological Evolution of the Volta River Delta (Ghana) and Yellow River Delta (China)

Previous studies mostly focus on an individual delta, or deltas at a global scale, to explore dam effects on deltaic hydrological alteration and morphological evolution, while comparative studies on selected similar deltas remain scarce. In this study, we compare the alteration of river discharge and sediment load, as well as the associated deltaic area and shoreline, of two deltas, namely, the Volta River Delta in Ghana and the Yellow River Delta in China, which are subject to similar forcings and mainstem dam influences. The results show that the sediment loads of the Volta River Delta and Yellow River Delta have decreased abruptly and gradually, respectively, to ~10% of the pre-dam level, presumably due to differences in reservoir capacity and upstream dam location. Sediment decline has led to a decrease of the fluvial dominance ratio, which has also been affected by the river mouth location and shoreline orientation. As a consequence, the area of the Volta River Delta has shifted to a new quasi-equilibrium, whereas the Yellow River Delta has kept prograding. This comparative study provides references for understanding the future evolution of similar deltas around the world.

Assessment of Water Measurements in an Irrigation Canal System Based on Experimental Data and the CFD Model

Due to their convenience, water measuring structures have become an important means of measuring water in irrigation canal systems However, relevant research on upstream and downstream water-depth monitoring point locations is scarce. Our study aims to determine the functional relationship between the locations of the water-depth monitoring points and the opening width of the sluice. We established 14 trunk-channel and branch-channel hydrodynamic models. The locations of the water-depth monitoring points for the upstream and downstream reaches and their hydraulic characteristics were assessed using a numerical simulation and hydraulic test. The results showed that the locations of the upstream and downstream water-depth monitoring points were, respectively, 16.26 and 15.51 times the width of the sluice. The average error between the calculated flow rate and the simulated value was 14.37%; the average error between the flow rates calculated by the modified and the simulated values was 3.36%. To further verify the accuracy of the modified discharge calculation formula, by comparing the measured values, we reduced the average error of the modified formula by 19.29% compared with the standard formula. This research provides new insights into optimizing water measurements in irrigation canal systems. The results provide an engineering basis for the site selection of water-depth monitoring points that is suitable to be widely applied in the field.

A New Multi Echelon Repair Network Model with Multiple Upstream Locations for Level of Repair Analysis Problem

Level of repair analysis (LORA) determines (1) the best decision during a malfunction of each product component; (2) the location in the repair network to perform the decision and (3) the quantity of required resources in each facility. Capital goods have long life cycles and their total life cycle costs are extremely high. LORA, which can be done repeatedly during the life cycle of the product, both at design and product support phase, plays an important role in minimising the total life cycle costs of capital goods. It is mostly applied to systems that operate in different geographical areas and deployed in different regions, which include different subsystems with special technology and expertise, and have a complex product structure. In this study, we propose a new mathematical model to the LORA problem, which is more comprehensive and flexible than the other pure LORA models in the literature. The proposed model uses the multiple upstream approach that allows the transfer of the components from a location in the lower echelon to the predefined locations in the upper echelon and determines the material movement paths between each facility, defining the facilities’ locations in the repair network. The performance of the proposed model is tested on benchmark instances and the results are compared with the single upstream model. Computational experiments show that the proposed model is more effective than the single upstream model and reduces the total life cycle costs by 4.85% on average, which is an enormous cost saving when total life cycle costs of capital goods are considered.

A complex-valued resonance model for axisymmetric screech tones in supersonic jets

We model the resonance mechanism underpinning generation of A1 and A2 screech tones in an under-expanded supersonic jet. Starting from the resonance model recently proposed by Mancinelliet al.(Exp. Fluids, vol. 60, 2019, p. 22), where the upstream-travelling wave is a neutrally stable guided-jet mode, we here present a more complete linear-stability-based model for screech prediction. We study temperature and shear-layer thickness effects and show that, in order to accurately describe the experimental data, the effect of the finite thickness of the shear layer must be incorporated in the jet-dynamics model. We then present an improved resonance model for screech-frequency predictions in which both downstream- and upstream-travelling waves may have a complex wavenumber and frequency. This resonance model requires knowledge of the reflection coefficients at the upstream and downstream locations of the resonance loop. We explore the effect of the reflection coefficients on the resonance model and propose an approach for their identification. The complex-mode model identifies limited regions of frequency–flow parameter space for which the resonance loop is amplified in time, a necessary condition for the resonance to be sustained. This model provides an improved description of the experimental measurements.

Lake sturgeon seasonal movements in regulated and unregulated Missouri River tributaries

AbstractSpatio‐temporal movement patterns of aquatic organisms drive many ecological processes. However, dams block migrations and alter the hydrologic and thermal regimes influencing movement behaviour of freshwater fishes. In North America, many recovering southern Lake Sturgeon populations occur in rivers with hydroelectric dams, but few studies have examined the impact of hydrologic alteration on their seasonal movements. We conducted a 3‐year telemetry study of 96 adult and subadult Lake Sturgeon to compare their migratory responses to temperature and hydrology in adjacent regulated and unregulated tributaries of the Missouri River. Many other populations of Lake Sturgeon use tributaries primarily for spring spawning; however, in our study, Lake Sturgeon used Missouri River tributaries during 78% of the year. Differences in river size, hydrologic and thermal regimes in the regulated Osage River may have contributed to the greater year‐round residency, later initiation, more frequent directional changes and longer duration of spring migrations compared to the unregulated Gasconade River. Lake Sturgeon made spring upstream migrations at temperatures of 13–19°C and elevated discharges in both rivers. However, Osage River migrants responded less to changes in discharge or temperature during spring migrations, especially those that overwintered at upstream locations. Fall tributary migrations occurred in the Osage River at rising or high discharges but were uncommon in the Gasconade River. Our identification of the influences of abiotic variables on the timing, duration and extent of Lake Sturgeon seasonal migrations can help guide management of habitat and hydrology in regulated rivers to recover migratory fishes globally.

Three‐Dimensional Subsurface Flow Path Controls on Flow Permanence

AbstractIntermittent streams currently constitute &gt;50% of the global river network, and the number of intermittent streams is expected to increase due to changes in land use and climate. Surface flows are known to expand and contract within the headwater channel network due to changes in the water table driven by climate, often changing seasonally. However, the underlying causes of disconnections and reconnections throughout the stream network remain poorly understood and may reflect subsurface flow capacity. We assess how 3D subsurface flowpaths control local surface flows at Gibson Jack Creek in the Rocky Mountains, Idaho, USA. Water table dynamics, hydraulic gradients, and hyporheic exchange were monitored along a 200‐m section of the stream throughout the seasonal recession in WY2018. Shallow lateral hillslope‐riparian‐stream connectivity was more frequent in transects spanning perennially flowing stream reaches than intermittent reaches. During low‐flow periods, larger losing vertical hydraulic gradients were observed in paired piezometers in intermittent reaches than in adjacent perennial reaches. Contrary to dominant conceptual models, longitudinal measurements of hydrologic exchange in both intermittent and perennial reaches were seasonally variable except for one perennial reach that showed consistent significant gains. Observed drying dynamics, as well as subsurface pathways, were highly variable even over short distances (30 m). Flow probability and subsurface flow capacity at upstream locations can be assessed with an outlet hydrograph and upstream flow measurements. Accurate characterization of subsurface storage, discharge, and connection is critical to understanding the drivers of drying cycles in intermittent streams and their likely responses to future change.

Scaling properties of the turbidity and streamflow time series at two different locations of an intra-Apennine stream: Case study

The time scales of a catchment hydrologic response in a human impacted intra-Apennine basin, was assessed by a power spectral analysis. Turbidity and streamflow data, at high resolution (10 min) have been considered during four years. The turbidity and streamflow have heavy tails that follow approximately Pareto distributions. Both also display scaling Fourier power spectra of the form f-b, over different scaling ranges: from 10 min to one day for the turbidity and from 1 h to 20 days for the streamflow data. For both locations the slope for the streamflow is close to b = 1.7, whereas for turbidity the values b = 1.68 and 2.25 are found for the upstream and downstream locations, respectively. The turbidity dataset at the downstream station has a steeper slope than the upstream station, implying a higher persistence of the turbidity fluctuations: this change of slope shows that the effect of the river channel and catchment is similar to a fractional integration from the upstream to the downstream data. Furthermore, the delay times of the turbidity dataset, and hence of suspended sediment, were calculated using a co-spectrum between the series and indicating characteristic transit times of 25 days and 145 days.

Genetic Characterization of the Tetracycline-Resistance Gene tet(X) Carried by Two Epilithonimonas Strains Isolated from Farmed Diseased Rainbow Trout, Oncorhynchus mykiss in Chile.

The main objective of this study was to characterize the tet(X) genes, which encode a monooxygenase that catalyzes the degradation of tetracycline antibiotics, carried by the resistant strains FP105 and FP233-J200, using whole-genome sequencing analysis. The isolates were recovered from fin lesion and kidney samples of diseased rainbow trout Oncorhynchus mykiss, during two Flavobacteriosis outbreaks occurring in freshwater farms located in Southern Chile. The strains were identified as Epilithonimonas spp. by using biochemical tests and by genome comparison analysis using the PATRIC bioinformatics platform and exhibited a minimum inhibitory concentration (MIC) of oxytetracycline of 128 µg/mL. The tet(X) genes were located on small contigs of the FP105 and FP233-J200 genomes. The sequences obtained for the tet(X) genes and their genetic environment were compared with the genomes available in the GenBank database of strains of the Chryseobacterium clade belonging to the Flavobacterium family, isolated from fish and carrying the tet(X) gene. The Tet(X) proteins synthesized by the Chilean Epilithonimonas strains showed a high amino acid similarity (range from 84% to 100%), with the available sequences found in strains belonging to the genus Chryseobacterium and Flavobacterium isolated from fish. An identical neighborhood of tet(X) genes from both Chilean strains was observed. The genetic environment of tet(X) observed in the two strains of Epilithonimonas studied was characterized by the upstream location of a sequence encoding a hypothetical protein and a downstream located alpha/beta hydrolase-encoding gene, similar to the observed in some of the tet(X) genes carried by Chryseobacterium and Flavobacterium strains isolated from fish, but the produced proteins exhibited a low amino acid identity (25–27%) when compared to these synthesized by the Chilean strains. This study reports for the first time the carriage of the tet(X) gene by the Epilithonimonas genus and their detection in fish pathogenic bacteria isolated from farmed salmonids in Chile, thus limiting the use of therapies based on oxytetracycline, the antimicrobial most widely used in Chilean freshwater salmonid farming. This results suggest that pathogenic strains of the Chryseobacterium clade occurring in Chilean salmonid farms may serve as important reservoirs of tet(X) genes.

Assessing potential health effect of lead and mercury and the impact of illegal mining activities in the Bonsa river, Tarkwa Nsuaem, Ghana

Access to quality water remains critical for protecting communities against waterborne diseases worldwide. Potable and quality water penetration to most rural communities is minimal such that over 2.2 billion people worldwide are negatively affected. Activities such as illegal mining, domestic use of water in river bodies, discharge of untreated household and industrial waste into water bodies, and agricultural pollution among others result in pollution of water bodies thus impacting water quality. This study sought to ascertain and measure the actual environmental impact of illegal mining activities in the Bonsa river in the Tarkwa Nsuaem Municipal Assembly, Ghana. The river was partitioned into upstream, midstream, and downstream locations, and water samples were collected and subjected to physicochemical and heavy metal pollution analysis. The physicochemical characteristics in the Bonsa river revealed mean turbidity values of 155.75 NTU downstream, 135.0 NTU midstream, and 207.0 NTU upstream which exceeds the required international benchmark of the World Health Organization (WHO) for potable water. The heavy metal analysis of lead and mercury also showed values considerably higher than those permissible by the Ghana Environmental Protection Agency (EPA) and the WHO indicating significant contamination levels. The study concludes that based on the physicochemical and heavy metal analysis, the Bonsa river is not safe for consumption at all locations and recommends water purification measures before use. The authors recommend the need for preventing related socioeconomic and public health implications through public education initiating plans towards the containment of water pollution.