Worst-case end-to-end delay analysis is important for real-time applications of switched Ethernet network. The Trajectory approach is one of the mature tools for worst-case delay analysis to calculate the upper bound of delay. The delay analysis based on the Trajectory approach as well as the packet serialisation of such networks is presented. An optimisation of packet serialisation is proposed in order to improve the Trajectory approach. A case study is illustrated to compare the improved Trajectory approach with the classical one, and the result shows that the improved Trajectory approach can get tighter upper bound of end-to-end delay.