Updated Reference Research Articles

Cell Temperature Determination based on IEC61215: Solar Photovoltaic Experimental Study of Tropical Malaysia

Most commercial photovoltaic (PV) module data sheets include Nominal Operating Cell Temperature (NOCT) values, which assist PV system designers in estimating module temperatures under real outdoor conditions. However, the typical NOCT values of 45°C to 47°C do not account for tropical climates. This study seeks to develop an adjusted NOCT mathematical model and determine revised NOCT values suited for tropical conditions. The new proposed NOCT model follows the international standard IEC61215 but incorporates updated tropical Standard Reference Environment (SRE) parameters: solar irradiance (SI) of 800 W/m², ambient temperature (AT) of 31°C, and wind speed (WS) of 1 m/s. This modified NOCT model demonstrates improved accuracy over the existing model, with an average reduction of 2% in percentage error, root mean square error, and mean average percentage error. Outdoor NOCT testing conducted in Shah Alam, Malaysia, uncovered much higher NOCT values for various PV modules technology: 55°C for monocrystalline, 57°C for polycrystalline, and 59°C for thin film. These results emphasize the inadequacy of current NOCT values in commercial PV module data sheets, which do not accurately reflect conditions in tropical climates. The revised NOCT values from this study offer crucial thermal reference data for PV system designers, integrators, and researchers working in tropical regions, particularly Malaysia.

Updated uranium reference material 231Pa/235U consensus model ages for nuclear forensics

Updated uranium reference material 231Pa/235U consensus model ages for nuclear forensics

Updating Pediatric Reference Ranges at Seattle Children’s Hospital Using an Indirect Approach

Abstract Background The aim of this project was to evaluate the accuracy of historical reference ranges at our institution. Our population of interest is children (<18 years old) within a large city. Ideally, reference intervals are determined using the direct method by performing studies on healthy volunteers. However, this approach has many challenges including being resource intensive, and, particularly in the pediatric population, difficulty in obtaining the required number of healthy volunteers for every age range. Given these challenges, a second approach, the indirect approach, has also been utilized to update reference ranges. This approach uses retrospective data analysis on data that was not specifically collected for evaluation of a reference range. Abnormal values are filtered out using a variety of methods. Methods For our project we pulled all outpatient data between 10/3/2020 and 06/30/2023 for the following analytes: albumin, ALP, AST, ALT, BUN, calcium, chloride, CO2, conjugated bilirubin, delta bilirubin, potassium, sodium, total bilirubin, total protein, and unconjugated bilirubin. We limited each patient to their first result for each analyte on the basis that analytes being measured more than once are more likely to be abnormal, and to limit bias. We also pulled the age (in days) and legal sex for each patient. The Tukey method was used to exclude outliers on the assumption that most data points would be normal, so any outliers would represent abnormal values from potentially not healthy patients. For each analyte and every age range the frequency of each value was plotted and the percentage of values within the current reference range, 2.5th percentile, and 97.5th percentile were calculated. A reference range was considered accurately representative of the normal population at our institution if the percentage of values within the reference range was greater than or equal to 80%. Results The “in range” percentage for albumin, ALT, BUN, calcium, chloride, conjugated bilirubin, delta bilirubin, creatinine, and total bilirubin was greater than 80% for all age ranges tested. Potassium, sodium, and unconjugated bilirubin each had one age range where the percent “in range” was less than 80% (2 days – 3 months, < 1 week, and < 1 month respectively), however the sample number for these age ranges was considered too low to make any significant conclusions (n=273, n=11, and n=21 respectively). ALP, AST, CO2, and total protein each had at least one age range where the percent “in range” was less than 80% and a substantial number of data points was available. In these cases, we proposed changing the reference interval to the calculated 2.5th and 97.5th percentiles based on our retrospective data analysis or verifying an externally validated pediatric reference interval from the CALIPER study.

An adaptive interval many-objective evolutionary algorithm with information entropy dominance

Interval many-objective optimization problems (IMaOPs) involve more than three conflicting objectives with interval parameters. Various real-world applications under uncertainty can be modeled as IMaOPs to solve, so effectively handling IMaOPs is crucial for solving practical problems. This paper proposes an adaptive interval many-objective evolutionary algorithm with information entropy dominance (IMEA-IED) to tackle IMaOPs. Firstly, an interval dominance method based on information entropy is proposed to adaptively compare intervals. This method constructs convergence entropy and uncertainty entropy related to interval features and innovatively introduces the idea of using global information to regulate the direction of local interval comparison. Corresponding interval confidence levels are designed for different directions. Additionally, a novel niche strategy is designed through interval population partitioning. This strategy introduces a crowding distance increment for improved subpopulation comparison and employs an updated reference vector method to adjust the search regions for empty subpopulations. The IMEA-IED is compared with seven interval optimization algorithms on 60 interval test problems and a practical application. Empirical results affirm the superior performance of our proposed algorithm in tackling IMaOPs.

State-Level Childhood Lead Poisoning Prevention Policies and Practices in the United States: 2022-2023.

The purpose of this analysis is to better understand laws and practices guiding prevention activities in childhood lead poisoning prevention programs funded by the Centers for Disease Control and Prevention (CDC). In 2022, CDC surveyed 62 funded programs using the Awardee Lead Profile Assessment. Information was collected about childhood lead poisoning-related laws and guidance, surveillance and prevention strategies, and program services including at what blood lead levels (BLLs) various activities are performed. Separately, CDC reviewed state health department websites to obtain information on which states implemented CDC's updated blood lead reference value. Awardee Lead Profile Assessment results are only reported for 47 states, the District of Columbia, and Puerto Rico. Almost all programs (96%) have laws requiring reporting of BLLs, and 51% require BLLs be reported electronically to jurisdictional health departments. Most programs (80%) prioritize areas and populations that are high risk for lead poisoning prevention activities. Approximately half of the programs (51%) have a lead elimination plan or goal. Thirty-nine percent of the programs have already implemented policies, laws, or regulations to achieve lead elimination, and 74% are in the Northeast and Midwest regions of the country. As of March 2023, 71% of the programs have implemented CDC's updated blood lead reference value, and most (65%) did so via guidance for health care providers and laboratories for what BLL should initiate case management and other services for lead-exposed children. Almost all programs have mandatory BLL reporting laws, and about two-thirds of the programs updated their BLLs that trigger public health action.

Implications of the 2022 lung function update and GLI global reference equations among patients with interstitial lung disease

BackgroundLung function testing remains a cornerstone in the assessment and management of interstitial lung disease (ILD) patients. The clinical implications of the Global Lung function Initiative (GLI) reference equations and...

Improved species assignments across the entire Anopheles genus using targeted sequencing.

Accurate species identification of the mosquitoes in the genus Anopheles is of crucial importance to implement malaria control measures and monitor their effectiveness. We use a previously developed amplicon panel (ANOSPP) that retrieves sequence data from multiple short nuclear loci for any species in the genus. Species assignment is based on comparison of samples to a reference index using k-mer distance. Here, we provide a protocol to generate version controlled updates of the reference index and present its latest release, NNv2, which contains 91 species, compared to 56 species represented in its predecessor NNv1. With the updated reference index, we are able to assign samples to species level that previously could not be assigned. We discuss what happens if a species is not represented in the reference index and how this can be addressed in a future update. To demonstrate the increased power of NNv2, we showcase the assignments of 1789 wild-caught mosquitoes from Madagascar and demonstrate that we can detect within species population structure from the amplicon sequencing data.

Updated reference values for BMD and lean mass measured by DXA in Thai children.

This study established normative references for total body less head (TBLH) BMD, lumbar spine (L1-L4) BMD, and both total and appendicular lean mass (LM) in Thai children and adolescents (aged 5-18years) using DXA. This work expands upon 2014 normative data for Thai children, which included L2-L4 BMD, total body BMD (head included), and total LM. We reanalyzed total body and lumbar spine DXA scans (Lunar Prodigy Pro, GE Healthcare; enCORE version 7.53) from 174 boys and 193 girls, using upgraded software (enCORE version 17SP2) for TBLH BMD, L1-L4 BMD, and LM analysis. The "enhanced" mode was applied for TBLH BMD and LM. Adjustments for total and appendicular LM were made relative to squared height (m2) to account for body size variability. Normative data stratified by sex and Tanner stage were generated for TBLH BMD, L1-L4 BMD, and LM indices. Weight and Tanner stage significantly determined BMD and LM. Adolescent girls exhibited higher LSBMD values due to earlier pubertal onset. Boys showed higher LM indices with more rapid gains during growth spurts. This study provides updated normative reference values for BMD (TBLH and L1-L4) and LM (total and appendicular) in Thai children and adolescents, measured via DXA. These references will enhance the assessment of low bone mass and LM deficits in Thai pediatric populations, particularly in those with chronic illnesses.

On the use of dioxane as reference for determination of the hydrodynamic radius by NMR spectroscopy

Measuring the compaction of a protein or complex is key to our understanding of the interactions within and between biomolecules. Experimentally, protein compaction is often probed either by estimating the radius of gyration (Rg) obtained from small-angle x-ray scattering (SAXS) experiments or the hydrodynamic radius (Rh) obtained, for example, by pulsed field gradient NMR (PFG NMR) spectroscopy. PFG NMR experiments generally report on the translational diffusion coefficient, which in turn can be used to estimate Rh using an internal standard to account for sample viscosity and uncertainty about the gradient strength. 1,4-Dioxane is one such commonly used internal standard, and the reference value of Rh is therefore important. We have revisited the basis for the commonly used reference value for the Rh of dioxane (2.12 Å) that is used to convert measured diffusion coefficients into a hydrodynamic radius. We followed the same approach that was used to establish the current reference value by measuring SAXS and PFG NMR data for a set of seven different proteins and using these as standards. Our analysis shows that the current Rh reference value for dioxane Rh is underestimated, and we instead suggest a new value of 2.27 ± 0.04 Å. Using this updated reference value results in a ∼7% increase in Rh values for proteins whose hydrodynamic radii have been measured by PFG NMR. These results are particularly important when the absolute value of Rh is of interest such as when determining or validating ensemble descriptions of intrinsically disordered proteins.

Select Venous Analytes and Fibrinogen Determination Using Two Methods in Brown Pelicans.

The brown pelican (Pelecanus occidentalis) is a species often affected by natural and man-made disasters such as hurricanes and oil spills, as well as general human activities; that subsequently receives medical care and rehabilitation. During rehabilitation, blood may be collected for various tests to help with diagnosis, treatment, and monitoring. Reference intervals for this species are limited, dated, and typically from small sample sizes. Seventy-one presumed healthy brown pelicans were sampled as part of their pre-release examination from rehabilitation at the Wildlife Center of Texas after a large volume stranding from December 2014 to January 2015, and various venous analytes were measured to establish updated reference intervals for brown pelicans. Fibrinogen was measured via heat precipitation and the Abaxis VSPro equine fibrinogen cartridge to determine reference intervals and in an attempt to validate the VSPro for use in avian species. Abaxis VS2 Avian/Reptile Chemistry panel, iSTAT CG4+, and iSTAT Chem8+ results, in addition to body condition score, spun PCV, cloacal temperature, and fibrinogen were measured. Proposed reference intervals for brown pelicans are presented. Fibrinogen results were not comparable between the gold standard method and the VSPro, indicating that the VSPro is not appropriate for use in brown pelicans.

Assessing Current Coastal Subsidence at Continental Scale: Insights From Europe Using the European Ground Motion Service

AbstractBeside climate‐change‐induced sea‐level rise (SLR), land subsidence can strongly amplify coastal risk in flood‐prone areas. Mapping and quantifying contemporary vertical land motion (VLM) at continental scales has long been a challenge due to the absence of gridded observational products covering these large domains. Here, we fill this gap by using the new European Ground Motion Service (EGMS) to assess the current state of coastal VLM in Europe. First, we compare the InSAR‐based EGMS Ortho (Level 3) with nearby global navigation satellite systems (GNSS) vertical velocity estimates and show that the geodetic reference frame used to calibrate EGMS strongly influences coastal vertical land velocity estimates at the millimeter per year level and this needs to be considered with caution. After adjusting the EGMS vertical velocity estimates to a more updated and accurate International Terrestrial Reference Frame (ITRF2014), we performed an assessment of VLM in European low elevation coastal flood plains (CFPs). We find that nearly half of the European CFP area is, on average, subsiding at a rate faster than 1 mm/yr. More importantly, we find that urban areas and populations located in the CFP experience a near −1 mm/yr VLM on average (excluding the uplifting Fennoscandia region). For harbors, the average VLM is even larger and increases to −1.5 mm/yr on average. This demonstrates the widespread importance of continental‐scale assessments based on InSAR and GNSS to better identify areas at higher risk from relative SLR due to coastal subsidence.

MISEV2023 provides an updated and key reference for researchers studying the basic biology and applications of extracellular vesicles.

The recently published "Minimal information for studies of extracellular vesicles - 2023 (MISEV2023)" in the Journal of Extracellular Vesicles has provided practical solutions to the numerous challenges extracellular vesicles (EVs) researchers face. These guidelines are imperative for novice and experienced researchers and promote unity within the EV community. It is strongly recommended that laboratories working with EVs make MISEV2023 an essential handbook and that researchers actively promote these guidelines during laboratory meetings, journal clubs, seminars, workshops, and conferences. A collective effort from EV researchers is crucial to steer the progress of EV science in a positive direction.

Detection of an 8p23.1 Inversion Using High-Resolution Optical Genome Mapping

Abstract Objective To evaluate the performance of optical genome mapping (OGM) in identifying an inversion located in the short arm of chromosome 8 (8p, 8p23.1), flanked by regions of complex segmental duplication (SD), using the GRCh38 and telomere-to-telomere (T2T) genome references. Methods We investigated a couple suspected of carrying the 8p23.1 inversion due to a terminal deletion combined with an interstitial duplication of 8p found in their abortus. OGM was performed on both individuals. The data were mapped to the current GRCh38 and the updated T2T genome references, respectively. Results The 8p23.1 inversion was observed in the female when mapping OGM data to the T2T assembly. In contrast, under the GRCh38 reference, the orientation between the suspected breakpoints within the SD regions could not be distinguished. Additional variants of uncertain significance were also identified in both individuals. Conclusion Our findings highlight the superiority of the T2T reference in recognizing structural variations involving SD regions. The enhanced SV detection using the T2T reference may contribute to a better understanding of genome instability and human diseases.

IGF-I assay methods and biologic variability: evaluation of acromegaly treatment response.

Serum insulin-like growth factor (IGF-I) is the primary biochemical measure of disease activity in patients with acromegaly, and the 2014 Endocrine Society guidelines recommended normal age-adjusted serum IGF-I as the biochemical target of treatment. However, quantification and interpretation of IGF-I levels are subject to limitations that may affect therapeutic decisions. Techniques for measuring IGF-I have evolved greatly over the past 40 years and continue to do so. Results can vary substantially for different assays, procedures, and laboratories. For any assay, the interpretation of IGF-I values requires robust reference ranges. Using currently available large normative databases, the upper limit of normal (ULN) for IGF-I in middle-aged and elderly individuals is lower than historical reference ranges. Thus, the goal of achieving IGF-I < 1× ULN is more demanding than in the past, and some patients with acromegaly who were classified as "normal" (IGF-I < 1× ULN) in previous studies would be reclassified as above the ULN based on newer normative data. In addition, substantial intra-individual, week-to-week variation in serum IGF-I levels (unrelated to assay performance) has been observed. With changes over time in the measurement of IGF-I and the advent of updated reference ranges derived from large normative databases, it is difficult to justify rigid adherence to the goal of maintaining IGF-I below the ULN for all patients with acromegaly. Instead, symptoms, comorbidities, and quality of life should be considered, along with growth hormone and IGF-I levels, when evaluating the need for further treatment.

An analysis of ChatGPT recommendations for the diagnosis and treatment of cervical radiculopathy

OBJECTIVE The objective of this study was to assess the safety and accuracy of ChatGPT recommendations in comparison to the evidence-based guidelines from the North American Spine Society (NASS) for the diagnosis and treatment of cervical radiculopathy. METHODS ChatGPT was prompted with questions from the 2011 NASS clinical guidelines for cervical radiculopathy and evaluated for concordance. Selected key phrases within the NASS guidelines were identified. Completeness was measured as the number of overlapping key phrases between ChatGPT responses and NASS guidelines divided by the total number of key phrases. A senior spine surgeon evaluated the ChatGPT responses for safety and accuracy. ChatGPT responses were further evaluated on their readability, similarity, and consistency. Flesch Reading Ease scores and Flesch-Kincaid reading levels were measured to assess readability. The Jaccard Similarity Index was used to assess agreement between ChatGPT responses and NASS clinical guidelines. RESULTS A total of 100 key phrases were identified across 14 NASS clinical guidelines. The mean completeness of ChatGPT-4 was 46%. ChatGPT-3.5 yielded a completeness of 34%. ChatGPT-4 outperformed ChatGPT-3.5 by a margin of 12%. ChatGPT-4.0 outputs had a mean Flesch reading score of 15.24, which is very difficult to read, requiring a college graduate education to understand. ChatGPT-3.5 outputs had a lower mean Flesch reading score of 8.73, indicating that they are even more difficult to read and require a professional education level to do so. However, both versions of ChatGPT were more accessible than NASS guidelines, which had a mean Flesch reading score of 4.58. Furthermore, with NASS guidelines as a reference, ChatGPT-3.5 registered a mean ± SD Jaccard Similarity Index score of 0.20 ± 0.078 while ChatGPT-4 had a mean of 0.18 ± 0.068. Based on physician evaluation, outputs from ChatGPT-3.5 and ChatGPT-4.0 were safe 100% of the time. Thirteen of 14 (92.8%) ChatGPT-3.5 responses and 14 of 14 (100%) ChatGPT-4.0 responses were in agreement with current best clinical practices for cervical radiculopathy according to a senior spine surgeon. CONCLUSIONS ChatGPT models were able to provide safe and accurate but incomplete responses to NASS clinical guideline questions about cervical radiculopathy. Although the authors’ results suggest that improvements are required before ChatGPT can be reliably deployed in a clinical setting, future versions of the LLM hold promise as an updated reference for guidelines on cervical radiculopathy. Future versions must prioritize accessibility and comprehensibility for a diverse audience.

HIVE TEMPERATURE REGULATION BY HONEYBEES IN RESPONSE TO EXTREME CONDITIONS

Honeybees are considered the primary pollinators of agricultural crops. However, climate change, pesticide use, disease transmission and pests all have an impact on their survival. Temperature is an important consideration, especially when bees are subjected to harsh conditions as a result of the effort required to regulate them within their hives. The brood chamber should be kept between 33 and 35 °C. The recognized temperature thresholds are 6 and 38 °C; deviations above or below these values cause metabolic damage in honeybees; the larger the difference, the greater the stress. These eusocial insects have developed thermoregulating mechanisms in response to adverse environmental conditions; the colony compensates for this difference by either fanning their wings to create air circulation and cause evaporative cooling or raising the temperature by generating endothermic heat to raise the temperature of the breeding chamber. This review brings together the most important and updated references on how to improve colony thermoregulation and its relationship to climate change.

Updated Reference Intervals for Alanine Aminotransferase in a Metabolically and Histologically Normal Population

Background and AimsThis study aims to reevaluate upper reference limit (URL) for alanine aminotransferase (ALT) by considering the changing epidemiology of major liver diseases. We employed histological and metabolic parameters in Asian living liver donors. MethodsWe performed a retrospective analysis of 5,455 potential living liver donors from 2005 to 2019. Participants were screened for hepatitis B, C, HIV, and alcohol use. Histologically and metabolically healthy participants were assessed using the Prati criteria (body mass index <23 kg/m2, triglyceride ≤200 mg/dL, fasting glucose ≤105 mg/dL, total cholesterol ≤220 mg/dL). The updated ALT-URL was determined as the 95th percentile among participants without hepatic steatosis and who met the Prati criteria. ResultsThe median age was 30 years, with a male predominance (66.2%). Among 5,455 participants, 3,162 (58.0%) showed no hepatic steatosis, with 1,553 (49.1%) meeting both the criteria for no steatosis and the Prati criteria for metabolic health. The updated URL for ALT in these participants was 34 U/L for males and 22 U/L for females, which was significantly lower than conventionally accepted values. Using this revised ALT-URL, 72.8% of males with ALT levels ≥34 U/L and 55.0% of females with ALT levels ≥22 U/L showed signs of steatosis, while 32.7% of males and 22.2% of females met the criteria for metabolic syndrome. ConclusionsOur study provided the newly established reference intervals for ALT levels in a metabolically and histologically verified Asian population. The proposed URL for ALT are 34 U/L and 22 U/L for males and females, respectively.

3D GIS PARTICIPATORY MAPPING AND CONFLICT LADM: COMPARATIVE ANALYSIS OF LAND POLICIES AND SURVEY PROCEDURES APPLIED BY THE IGOROTS, NCIP, AND DENR TO ITOGON ANCESTRAL DOMAIN BOUNDARIES

Abstract. Based on the 2015 census, the Indigenous Peoples (IPs) population in the Philippines is estimated to range from 11.3 to 20.2 million. Spanning diverse regions with unique cultures, these communities face common challenges related to territorial disputes. Itogon, the largest municipality in Benguet, is the ancestral home of the Ibaloi, Kankanaey, and other Igorot tribes. Despite possessing three Ancestral Domains (ADs), Itogon is predominantly classified as timberland, exposing inconsistencies in national laws and jurisdiction. This study examines land policies and surveying procedures employed by the Igorots, National Commission on Indigenous Peoples (NCIP), and Department of Environment and Natural Resources (DENR) in mapping Itogon's AD Boundaries. It aims to identify conflicts in land boundary delineation, propose surveying guidelines, and advocate for 3D Participatory Mapping as a geomatics solution for updated AD reference maps. Three methodologies were used: Interpretative Phenomenological Analysis (IPA), Comparative Legal Analysis (CLA), and Map Overlay Analysis (MOA). IPA revealed key themes, including AD Definition, AD Overlaps, and Other Conflicts, pertinent to AD mapping. CLA delved into conflicting surveying procedures, such as AD Definitions, Surveying Equipment, Methods, and more. MOA highlighted the land area percentage affected by misaligned surveying procedures. The study presents its findings through a Conflict Land Administration Domain Model (LADM) for AD versus AD and Political Boundaries. These can serve as references for revising survey manuals, updating AD Sustainable Development and Protection Plans, and making amendments to laws, all aimed at promoting indigenous land rights and sustainable development.

A first‐order hyperbolic arbitrary Lagrangian Eulerian conservation formulation for non‐linear solid dynamics

SummaryThe paper introduces a computational framework using a novel Arbitrary Lagrangian Eulerian (ALE) formalism in the form of a system of first‐order conservation laws. In addition to the usual material and spatial configurations, an additional referential (intrinsic) configuration is introduced in order to disassociate material particles from mesh positions. Using isothermal hyperelasticity as a starting point, mass, linear momentum and total energy conservation equations are written and solved with respect to the reference configuration. In addition, with the purpose of guaranteeing equal order of convergence of strains/stresses and velocities/displacements, the computation of the standard deformation gradient tensor (measured from material to spatial configuration) is obtained via its multiplicative decomposition into two auxiliary deformation gradient tensors, both computed via additional first‐order conservation laws. Crucially, the new ALE conservative formulation will be shown to degenerate elegantly into alternative mixed systems of conservation laws such as Total Lagrangian, Eulerian and Updated Reference Lagrangian. Hyperbolicity of the system of conservation laws will be shown and the accurate wave speed bounds will be presented, the latter critical to ensure stability of explicit time integrators. For spatial discretisation, a vertex‐based Finite Volume method is employed and suitably adapted. To guarantee stability from both the continuum and the semi‐discretisation standpoints, an appropriate numerical interface flux (by means of the Rankine–Hugoniot jump conditions) is carefully designed and presented. Stability is demonstrated via the use of the time variation of the Hamiltonian of the system, seeking to ensure the positive production of numerical entropy. A range of three dimensional benchmark problems will be presented in order to demonstrate the robustness and reliability of the framework. Examples will be restricted to the case of isothermal reversible elasticity to demonstrate the potential of the new formulation.

Updated adult ferritin reference intervals based on a large, healthy UK sample, measured on Roche Cobas series analysers.

There is a lack of standardization of reference intervals (RIs) for ferritin across laboratories, particularly for postmenopausal women. Depending on the RI used, there can be more than a 4-fold difference in the upper limit of normal between laboratories, resulting in potential misinterpretation. This retrospective study used a large dataset of blood test results from 25,425 healthy participants aged 18 to 97 over a 7-year period. Exclusion criteria were used to screen out individuals with conditions known to affect iron metabolism or raise ferritin as part of the acute phase response. Distributions were assessed using density and Q-Q plots, and age-banded cut-offs were determined. The non-parametric method was used to establish RIs for sex and age bands. For females, 4 age bands were established (18-39, 40-49, 50-59 and 60+). For males, 2 bands were identified (18-39 and 40+). Performance against a validation dataset, followed by an expansive validation against an inclusive dataset, demonstrated the robustness of the derived RIs. This study addresses the inconsistency in serum ferritin RIs by presenting intervals based on demographic parameters. This approach can potentially enhance the accuracy of interpreting serum ferritin levels, assisting clinicians in identifying patients requiring further evaluation.