Untreated Soil Research Articles

Soil zinc dynamics over time on applied organics, bacterial inoculant and zinc fertilizers in calcareous soil of southern India

Bioavailability of zinc was impacted by soil properties, externally applied sources, time, and various fractions of zinc. An experiment was conducted to investigate the bioavailable and other zinc fractions in calcareous soil and the efficiency of organic and inorganic sources on bioavailability with the presence and absence of zinc solubilizing bacteria (ZSB). The sequential extraction procedure followed at every interval of the incubation period for all forms of zinc was studied. Externally added inorganic sources significantly affected all fractions of zinc compared to untreated soils. Among them, ZnSO4 influences all forms of zinc, mainly Ws+Ex (water soluble+exchangeable) and carbonate-bound zinc, whereas Zn-EDTA maintains a high status of bioavailable zinc throughout the experimental period. On the 60th day of incubation, Zn-EDTA and ZnSO4 applied to soil maintained bioavailable zinc content of 3.71 and 2.94 times higher than that of control. Irrespective of sources, the available zinc of Zn fertilizers applied to soils was reduced with an increase in incubation days. Organic and microbial addition effects solely or combined increase the soil zinc content significantly in both fertilized and unfertilized soils. Among two different organic sources, the zinc solubility performance of farmyard manure was higher than that of vermicompost. In untreated soil, residual and carbonate-bound fractions contribute a major portion towards total zinc based on quantity. The bioavailable fraction mainly the Ws+Ex and organically bound fractions were markedly influenced in all treatments.

Enhanced remediation of acetochlor-contaminated soils using phosphate-modified biochar: Impacts on environmental fate, microbial communities, and plant health

Given that acetochlor (ACT) persists in soil for extended periods, disrupting microbial community structure and causing phytotoxicity to sensitive crops, this study investigated the potential of phosphate-modified biochar (PBC-800) to remediate ACT-contaminated soil. Incorporating 0.5 % PBC-800 into fluvo-aquic, red, and black soils increased their adsorption capacities by 80.4 mg g−1, 76.6 mg g−1, and 76.0 mg g−1, respectively. Even after six months of aging, the Kf values remained 1.6 to 5.1 times higher than in untreated soils. PBC-800 also accelerated ACT degradation across all three soil types, reducing residual ACT levels by 34.3 % to 76.4 % after 60 days, and shortening the degradation half-life by 5 to 7 days. High-throughput sequencing revealed that ACT reduced soil microbial diversity and disrupted community structure, while 0.5 % PBC-800 amendments promoted the growth of degradation-capable genera such as Rhodococcus, Lysobacter, and Gemmatimonas, enhancing microbial ecosystem stability. Furthermore, the amendment of soil with 0.5 % PBC-800 reduced ACT residue concentrations in maize and soybeans by 76.5 % to 82.9 %, and restored plant biomass, leaf chlorophyll content, and mesophyll cell ultrastructure to levels comparable to the control. Therefore, amending ACT-contaminated soil with PBC-800 mitigates ecological and environmental risks, boosts microbial activity, and safeguards plant health.

Foundation Model Study on Inorganic and Biopolymer Nano Additives Treated Soil—A Step Forward in Nano Additive Soil Stabilization

For almost a decade, various studies have been carried out to prove the suitability of nano additives in enhancing the geotechnical properties of soil. Yet, this line of research is still in its elementary stage, restricting itself to laboratory tests to determine soil’s index and engineering properties blended with varying dosages of nano additives. In other words, research on practical applications of nano additives for soil stabilization is scarce. The present work attempts to investigate the suitability of three different nanomaterials as a load-bearing stratum for shallow foundations. The nano additives were chosen in such a way that each of them is from a different origin. One of them is nano calcium carbonate (inorganic) whereas the other two are nano-sized varieties of natural biopolymers, namely nano chitosan (crustacean-based) and nano carboxymethyl cellulose (plant-based). A series of laboratory tests were initially conducted to determine the strength of all three nano-additive-treated soils at different dosages, which were investigated for 180 days to ensure their long-term performance. This was followed by a foundation model study on untreated soil and on soil treated with optimal dosages of nano additives. The results were validated using finite element software followed by a parametric study to optimize the depth of soil stabilization. It was observed that all three nano additives exhibited a better performance when the top layer had the optimal dosage and the subsequent layers had a relatively lesser dosage.

Effects of Microbial Organic Fertilizer, Microbial Inoculant, and Quicklime on Soil Microbial Community Composition in Pepper (Capsicum annuum L.) Continuous Cropping System

The additions of microbial organic fertilizer (MOF), a microbial inoculant (MI), and quicklime (Q) are considered to be sustainable practices to restore land that has been damaged by continuous cropping of pepper (Capsicum annuum L.). However, the combined effects of these three additives on pepper yield, soil chemical properties, and soil microbial communities were unclear. The experimental design consists of 13 treatment groups: the untreated soil (control); soil amended solely with three treatments for each of MOF (1875–5625 kg ha−1), MI (150–450 mL plant−1), and Q (1500–4500 kg ha−1); and soil amended with combinations of MOF, MI, and Q at three comparable concentrations. A significant increase in pepper fruit diameter, length, yield, and soil available nitrogen, phosphorus, and potassium contents occurs upon exclusive and combined applications of MOF, MI, and Q. Pepper yield was greatest (29.89% more than control values) in the combined treatment with concentrations of 1875 kg ha−1 MOF, 150 mL plant−1 MI, and 1500 kg ha−1 Q. The application of Q increased soil pH and reduced soil–fungal richness. The application of MOF, MI, and Q increased the relative abundance of bacterial genera and the complexity of bacterial and fungal co-occurrence networks compared with control levels. The combined application of MOF, MI, and Q resulted in the greatest microbial network complexity. A Mantel test revealed the key role of soil available nitrogen content and bacterial diversity in the regulation of pepper growth and yield. We conclude that the combined application of MOF, MI, and Q improves soil nutrient availability and modifies soil microbial community composition, significantly promoting plant growth and pepper yield during continuous cultivation.

Understanding and mitigating settlement in gypseous soils: A comprehensive study on the role of saturation and soil stabilization techniques

This study examines the influence of saturation levels and soil additives on the load-settlement properties of gypseous soils, a significant geotechnical concern in regions like Iraq. The study analyzes the deficiencies of conventional oedometer testing, which often fail to adequately represent the true unsaturated conditions prevalent in these soils. The research investigates the impact of varying matric suction (Ψ) values on the compressibility of untreated, cement-treated, and CKD-treated gypseous soils. A modified oedometer apparatus, engineered to control and quantify matric suction, was used to replicate diverse moisture conditions. The experimental program investigated soils with varying gypsum contents (8.8%, 12.66%, and 30%), reflecting the diversity of gypseous soils seen in the field. The findings demonstrate that increased saturation levels significantly enhance settling in gypseous soils. As matric suction increases, settlement is markedly reduced due to elevated soil suction pressure and enhanced effective stress. The findings underscore the importance of unsaturated conditions in improving the load-bearing capacity of these soils. The study illustrates the advantageous impacts of cement and CKD treatments in reducing settling. The effectiveness of these methods is particularly apparent in partially saturated environments, emphasizing the need of including matric suction into design considerations. This research improves understanding of the complex behavior of unsaturated gypseous soils. The findings have practical importance for geotechnical engineering, including foundation design, ground improvement methods, and the development of effective solutions for settlement issues in areas with high gypsum concentrations.

Synergistic bioremediation of petroleum-contaminated soil using immobilized consortium of Rhodococcus rhodochrous and Bacillus subtilis laccase

Petroleum-contaminated soil represents a significant environmental and public health challenge on a global scale. Microbial bioremediation has shown potential, yet the role of enzymes in enhancing petroleum degradation remains underexplored. In this study, the synergistic effects of Rhodococcus rhodochrous (R.rh) and Bacillus subtilis-derived laccase (BsLac) was investigated in the remediation of petroleum-contaminated soil. Immobilized R.rh (PSIMRH) and BsLac (ADIMLac) exhibited higher petroleum degradation rates than their free state, achieving 78.3% and 56.3% degradation in liquid systems, respectively. The combined treatment of PSIMRH and ADIMLac demonstrated a synergistic effect on petroleum degradation, achieving 43.6% with a maximum degradation constant of 0.0335 d−1, representing a 202.7% improvement over untreated soil. PSIMRH enhanced petroleum degradation through microbial metabolism, while ADIMLac accelerated the initial breakdown of complex hydrocarbons into simpler, more bioavailable ones via enzymatic oxidation, providing growth substrates for microbes and significantly improving petroleum degradation rates. The microbial analysis revealed an increase abundance of known petroleum-degrading bacterial genera, including Rhodococcus, Lysobacter, Micromonospora, and Streptomyces. However, the presence of BsLac appeared to reduce the competitive advantage of Rhodococcus, promoting the proliferation of indigenous strains like Lysobacter and Streptomyces. These results suggest that enzyme-microbe synergy can enhance the bioremediation process by altering microbial community dynamics and accelerating petroleum degradation. This study attempts to remediate petroleum-contaminated pollution with the combined use of strains and enzymes, providing a new approach for the remediation of other pollution problems.

Effect of ozone on Vibrio removal in a simulated earthen shrimp pond

ABSTRACT This study investigated the efficacy of ozone treatment on Vibrio pathogen removal within a simulated earthen shrimp pond, conducted in three phases. First, physical and chemical properties of the soil, alongside the Vibrio pathogen, were assessed. Results indicated neutral pH levels, high organic matter, and organic carbon content, with a Vibrio pathogen load of 1.0 ± 0.0 × 103 CFU/mg. Second, ozone treatment was applied, comparing its effectiveness in Vibrio pathogen control between treated and untreated soil sets. The treated set exhibited a significantly lower Vibrio pathogen load (6.00 ± 1.41 × 103 CFU/mg) compared to the untreated control (2.00 ± 2.12 × 105 CFU/mg), resulting in a 97.23% eradication efficiency. Concurrently, ammonia rates decreased with ozone, indicating potential benefits for shrimp aquaculture. Finally, ozone application in a simulated earthen pond over 45 days effectively controlled Vibrio pathogens. In the untreated soil set, Vibrio pathogen levels rose to 9.48 ± 1.73 × 105 CFU/mg, while in the ozone-treated, they ranged from 6.5 ± 2.12 × 103 to 1.25 ± 0.29 × 105 CFU/mg. Shrimp growth parameters, including average daily gain, survival rates, and feed conversion ratio, were compared between groups, suggesting ozone treatment's feasibility without adverse effects on shrimp growth. Water quality parameters remained within suitable ranges for shrimp cultivation. These findings highlight ozone's potential as an effective method for Vibrio pathogen control in shrimp aquaculture, with implications for industry sustainability and productivity.

Regulating facility soil microbial community and reducing cadmium enrichment in lettuce by reductive soil disinfestation

The accumulation of cadmium (Cd) in facility soil is attracting increasing attention. Reductive soil disinfestation (RSD) is an effective soil disinfection method, while also having a certain passivating effect on Cd. The application of organic matter is crucial for the success of RSD, but the reduction efficiencies of different organic materials vary. Here, five treatments, namely untreated soil (CK), bean dregs (BD), peanut dregs (PD), sesame dregs (SD), and apple dregs (AD) were applied, and their performances in Cd immobilization in Cd-contaminated soil were assessed. Changes in soil properties and microbial communities were monitored. The results showed that the overall soil pH following RSD treatment decreased significantly, while organic matter, hydrolyzed nitrogen, available phosphorus, available potassium, exchangeable calcium, and exchangeable magnesium increased significantly. Compared to CK, the exchangeable (EX)-Cd contents after treatments SD and AD significantly decreased by 25.4% and 23.7%, respectively. RSD led to significant changes in the composition of soil microbial communities. SD treatment significantly increased the number of soil fungal operational taxonomic units (OTU), while BD, PD, and SD significantly increased the relative abundances of Bacteroidetes, Chloroflexi, Deinococus Thermus, and Basidiomycota in bacterial communities. The Gemmatimonadetes phylum showed a highly significant negative correlation with EX-Cd, indicating that it might have a positive effect on the fixation of Cd in polluted soil. SD significantly reduced the Cd content in the above-ground parts of lettuce by 74.76%, and had the least impact on lettuce biomass. It can be inferred that RSD is an ecologically effective method for the remediation of Cd pollution in facility soil by improving soil characteristics and altering microbial community composition to reduce Cd activity. However, further in-depth research is needed to optimize the types and amounts of organic materials applied.

Investigation of small-strain shear modulus of marine sediment treated with different flocculants

Dredged sediments are typically found near coastal areas. Because of its high compressibility potential and low strength, multiple studies have tested various substances that can be mixed with the dredged materials to enhance the overall treatment process while minimizing post-construction settlement. Recent investigations indicated that combining flocculants such as cationic polyacrylamide (CPAM) with marine sediment improves the consolidation and permeability properties compared to untreated soil. Despite its importance, only a few studies have focused on the mechanical properties of marine sediment treated with various flocculant types. This study employs a bender element apparatus to examine the small-strain shear modulus (Gmax) variation of Macau marine sediment treated with three different flocculants: CPAM; chitosan; and nanoscale zero-valent iron (nZVI). The result revealed that the Gmax of natural marine sediment increases when blended with chitosan and CPAM with medium cationicity. Interestingly, no apparent change in Gmax values was observed when the marine sediment samples were treated using CPAM with high cationicity. In contrast, the Gmax values of soil samples treated with nZVI decreased. The comparison with existing empirical models reveals that the plasticity index is a significant parameter in determining the Gmax of Macau marine sediments. The findings from this study will support the identification of viable filling materials for future land reclamation projects in the Greater Bay Area of China.

Reducing drought vulnerability of forest soils using Xanthan gum-based soil conditioners

Climate change increases the frequency and severity of droughts in many parts of Europe, thereby affecting the availability of water resources. Therefore, preserving the soil water content is essential for maintaining forest diversity and plant vitality. To improve soil hydraulic properties and reduce drought vulnerability, three xanthan-gum-based soil conditioners (SC_R, SC_CG, and SC_ZZC) were developed under the European ONEforest project. These soil conditioners (SCs), including oxide ash and cellulose fibres of different lengths, differ in their filler properties. This study evaluated the performance of these soil conditioners in forest soils in Slovenia (S1), Spain (S2), and Germany (S3). Water absorption, water retention, hydraulic conductivity, seed germination, and plant growth in the untreated soils and mixtures were analysed.The results showed a 53% to 100% increase in water absorption with high dosage of SCs (1.7% of dry SC per dry soil mass). The addition of SCs also significantly improved the water retention capacity of the treated soils within a suction range of 0-100 kPa, which is advantageous for maintaining adequate water availability for plant growth. The effect of SCs on unsaturated hydraulic conductivity varies depending on the soil type. For example, at a similar suction, the unsaturated hydraulic conductivity of mixtures with soils S2 and S3 can be more than an order of magnitude lower than that of untreated soils S2 and S3. In contrast, mixtures with soil S1 showed unsaturated hydraulic conductivity similar to that of the untreated soil. In the seeding experiment, plants in treated soils survived for up to 8 d without watering compared to those in untreated soils, with survival linked to the initial water content of the mixtures. These findings suggest that soil conditioners reduce drought vulnerability by improving water retention and regulating water loss. However, the optimal dosage should be adjusted according to different soil types and local environmental conditions.

Enhancing clay soil reinforcement using the MICP-BIN method with biochar-induced nucleation

The microbially induced carbonate precipitation (MICP) method has gained extensive use in the realm of soil stabilization. The combination of MICP with biochar offers potential benefits in terms of simultaneous strength enhancement and vegetation cover enhancement. This study presents a novel microbial solidification technique known as the biochar-induced nucleation (MICP-BIN) method for reinforcing clay soil. Corn stover biochar was employed as an auxiliary material, mixed with dry clay powder, and subsequently reinforced using the MICP technique. Direct shear tests (DSTs), drying experiments, X-ray diffraction (XRD), and scanning electron microscopy (SEM) were also conducted to investigate the efficacy of the MICP-BIN method. Compared with that of the remolded soil, the shear strength of the treated soil exhibited a substantial increase of 22.4%, the CaCO3 content increased by 30%, and the shear strength improved by 389.5%. Similarly, the internal friction angle exhibited increases of 22.7% and 405.2%, while the cohesion showed improvements of 9.4% and 344.3%, respectively. The MICP-BIN method is found to increase suction for a given water content. Further, biochar amended soil possesses highest water retention ability (especially at lower suction magnitude) followed by MICP amended soil, MICP-BIN amended soil and untreated soil. The difference in water retention abilities among various soils is negligible at higher suction magnitude. Further, there is no significant change in air entry value of MICP-BIN amended soil as compared to the untreated soil and MICP amended soil. This study demonstrated the promising results achieved by combining biochar and the MICP-BIN technique, providing a novel approach for reinforcing soft ground.

Effects of inoculation with Bacillus arenosi on physical and biochemical properties of soil, surface runoff and erosion in degraded forestlands of Northern Iran

A viable practice to improve key properties of soil, and reduce runoff generation and erosion may be inoculation with selected bacteria. However, no previous studies have evaluated whether the direct inoculation of Bacillus arenosi K64 (hereafter simply indicated as B. arenosi) to soil impacts the main physical and biochemical properties of soil as well as the runoff and erosion rates. This study has measured the infiltration rate (IR), tensile strength (TS), mean weight diameter (MWD), polysaccharides (PS) and important enzymes of soil (β-glucosidase, N-acetyl glucosaminidase, phosphatase and arylsulphatase) in degraded forest soils (Northern Iran) inoculated with B. arenosi in comparison to untreated soils (control). Moreover, the runoff coefficient and soil loss have been measured in plots with soil sampled in those sites under natural rainfalls. IR, TS and MWD increased by 65 % to 160 % in treated soils compared to the control. PS and enzymes were significantly higher (by 20 % to 200 %) after soil inoculation. A noticeable decrease in both surface runoff and soil loss (both by 45 %) in the inoculated soils resulted from those changes. High correlations between the hydrological response of soil on one side, and the measured physical and biochemical variables on the other side were observed. A derivative variable calculated by the Principal Component Analysis can be considered as a meaningful parameter to estimate the changes in soil properties and response due to inoculation with bacteria. Agglomerative Hierarchical Cluster Analysis clearly discriminated the treated from the untreated soils. This study has demonstrated that soil inoculation with B. arenosi plays a significant role in reducing runoff generation and soil erosion under the experimental conditions, thanks to the general improvement in physical and biochemical properties of soil.

Manure-biochar compost mitigates the soil salinity stress in tomato plants by modulating the osmoregulatory mechanism, photosynthetic pigments, and ionic homeostasis

One of the main abiotic stresses that affect plant development and lower agricultural productivity globally is salt in the soil. Organic amendments, such as compost and biochar can mitigate the opposing effects of soil salinity (SS) stress. The purpose of this experiment was to look at how tomato growth and yield on salty soil were affected by mineral fertilization and manure-biochar compost (MBC). Furthermore, the study looked at how biochar (organic amendments) work to help tomato plants that are stressed by salt and also a mechanism by which biochar addresses the salt stress on tomato plants. Tomato yield and vegetative growth were negatively impacted by untreated saline soil, indicating that tomatoes are salt-sensitive. MBC with mineral fertilization increased vegetative growth, biomass yield, fruit yield, chlorophyll, and nutrient contents, Na/K ratio of salt-stressed tomato plants signifies the ameliorating effects on tomato plant growth and yield, under salt stress. Furthermore, the application of MBC with mineral fertilizer decreased H2O2, but increased leaf relative water content (RWC), leaf proline, total soluble sugar, and ascorbic acid content and improved leaf membrane damage, in comparison with untreated plants, in response to salt stress. Among the composting substances, T7 [poultry manure-biochar composting (PBC) (1:2) @ 3 t/ha + soil-based test fertilizer (SBTF)] dose exhibited better-improving effects on salt stress and had maintained an order of T7 > T9 > T8 > T6 in total biomass and fruit yield of tomato. These results suggested that MBC might mitigate the antagonistic effects of salt stress on plant growth and yield of tomatoes by improving osmotic adjustment, antioxidant capacity, nutrient accumulation, protecting photosynthetic pigments, and reducing ROS production and leaf damage in tomato plant leaves.

Impact of polyvinyl alcohol application and wheat straw mulching on soil loss and infiltration rate in semi-arid tropics

A study was conducted at Punjab Agricultural University, Ludhiana, Punjab, with the aim of monitoring soil loss and infiltration rate in loamy sand soil. The study focused on the effects of applying polyvinyl alcohol (PVA) and mulch under simulated rainfall conditions. The experimental setup involved testing three levels of PVA (0.5%, 0.75%, and 1.0%), one level of wheat-straw mulch (600 g/m2), and a Control treatment (untreated soil). Each of these treatments was replicated four times. The lowest soil loss (20.9 g/m2) was recorded under the 1.0% PVA treatment, while the highest (120.1 g/m2) was seen under the 0.5% PVA treatment. The 1.0% PVA treatment showed a significant reduction in soil loss compared to the 0.5% PVA, 0.75% PVA, mulch, and Control treatments, with reductions of approximately 82.6%, 45.1%, 81.2%, and 89.6%, respectively. Regarding infiltration rates, the Control treatment exhibited the lowest rate (2.4 cm/h), while the 1.0% PVA treatment displayed the highest rate (9.6 cm/h). Additionally, the use of mulch led to a 44.7% reduction in soil loss compared to the treatment without mulch, likely due to the mitigated impact of raindrops. The infiltration rate was significantly higher (4.8 cm/h) under the mulched treatment compared to the unmulched treatment (2.4 cm/h). Overall, the application of PVA and mulch resulted in a drastic reduction in soil loss, likely attributable to the enhanced stability of soil aggregates, improved infiltration rate, and reduced runoff.

Changes in rill detachment capacity after deforestation and soil conservation practices in forestlands of Northern Iran

Rill erosion is particularly intense after deforestation on hillslopes with steep and long profiles. Since the rill detachment capacity (“Dc”) shows a noticeable variability resulting from different soil properties and vegetation characteristics, there is a need to explore the changes in key soil properties and rill erosion rates under different natural or planted species and a variety of soil conservation practices after deforestation. This study evaluates the changes in four key soil properties (organic carbon content, bulk density and water-stable aggregates of soil, and weight density of plant roots) and rill detachment capacity in forest sites with natural tree species as well as in areas subjected to reforestation and soil conservation treatments in comparison to deforested sites. To this aim, 2000 soil samples have been collected in forestlands of Northern Iran. Sampling was done in deforested areas (assumed as reference condition), and in forest sites 14 plant species (natural or after reforestation). Deforested samples were subjected to five soil conservation treatments (using additives or hydromulching). On all soil samples, Dc has been measured in a laboratory flume at five water flow rates (0.27 to 0.69 L/m s−1) and five soil slopes (5.9 to 31.7 %). All soil properties, when compared to the reference condition, were significantly different (between −50 % to 124 %) among the three soil conditions (natural forests, reforested soils and deforested and treated sites), the natural forests and the treated sites showing a better quality compared to planted forests. Dc was noticeably lower in all conditions (0.022 ± 0.021 kg m−2 s−1) compared to the deforested and untreated sites (0.046 ± 0.023 kg m−2 s−1). Natural and planted forests showed a similar decrease in rill erodibility (−70 to 80 %), while a much lower reduction in Dc (−36 %) was measured for the treated areas. Overall, the study demonstrates that the changes in soil properties due to plant species and soil management as well as the associated variability in rill detachment are noticeably site-specific, and greatly depend on soil conservation treatments. The results of this study may give landscape planners clear indications about the relationships between rill detachment and the associated soil properties among treated and untreated (natural or reforested) soils.

Monitoring of indicators and bacterial succession in organic-amended technosols for the restoration of semiarid ecosystems

Restoration of mining sites is essential to ensure ecosystem services and biodiversity. One restoration strategy employed in arid and semi-arid zones is the use of organic amendments to establishment technosols. However, it is necessary to monitor the restoration progress in order to select appropriate amendments. This study monitored the effects of compost gardening, greenhouse horticulture and stabilized sewage sludge, and their blends. We focused on soil physical and chemical indicators and bacterial community structure and diversity during the 30 months after application. Organic amendments increased total organic carbon and nitrogen within six months, staying elevated compared to natural soils over 30 months. Electrical conductivity rose then stabilized, the pH slightly decreased but stayed alkaline, and water holding capacity improved in treated technosols. Bacterial diversity increased in amended technosols compared to control. Alpha diversity varied with treatment and time, peaking at 18 months. Technosols with plant compost showed reduced bacterial richness at 30 months, while those with sewage sludge and its mixtures maintained it. The bacterial community analysis showed significant differences among treatments and times, highlighting dominant phyla like Proteobacteria and Bacteroidetes. PCoA analysis showed clear separation of bacterial communities from treated, natural, and control soils, with notable differences between plant and sludge treatments. Soil variables such as TOC, TN, EC and water holding capacity explained more than 82 % of the variation in bacterial communities. Eighty-three indicator taxa were identified that explained the differences between the microbial communities of treated and untreated soils, highlighting the importance of taxa such as Pelagibacterium spp., Roseivirga spp. and Cellvibrio spp. in preserving soil health. In short, organic amendments improve soil properties and promote the diversity and stability of beneficial microbial communities in semi-arid mined soils, underlining their crucial role in the restoration and long-term maintenance of degraded soils.

Microstructural analysis and prediction of strength response of black expansive soil

Amelioration studies using waste residues are gaining popularity in the construction sector, as they offer dual effective management and utilization of waste residue. This practice is of interest to the growth of any nation due to environmental protection and the economic advantages it offers. For that reason, this present-day study seeks the sustainable utilization of calcium carbide residue (CCR) and palm oil fuel residue (POFR) in expansive soil amelioration. After an in-depth characterization of the parent soil, several number of California bearing ratio (CBR) tests was performed on the parent and additive-treated soil. For this purpose, a total of twenty mixes were prepared possessing various combinations of CCR, POFR, water, and soil. CBR testing for soaked and unsoaked conditions was carried out to assess the strength performance and effectiveness of the additives. Through this approach, optimal California bearing ratio (CBR) values of 41 and 61% were achieved at trial runs designated Y4 for soaked and unsoaked conditions, indicating a strong mechanical performance. These experimental outcomes were subsequently validated using statistical indicators such as ANOVA (F crit > F) and student t-test (t crit > t stat). A step further, microstructural testing such as transmission electron microscopy (TEM), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) were performed on the natural soil and soil treated with the optimal level of additives obtained at trial runs designated Y4. The SEM micrographs evidence the transformation of the loosely packed structure of untreated soil into a dense soil composite. Similarly, diverging molecular functional groups were recorded in the FTIR examination with the incorporation of blended CCR-POFR. Finally, the findings suggest that carefully designed mixtures of CCR and POFR can offer a sustainable, efficient approach to soil amelioration, potentially revolutionizing construction practices.

Effect of kaolin-nano-silica mixture on geomechanical properties enhancement of soils

Weak soil is a major obstacle facing the urban development of any site with other exceptional merits. The current study aims to investigate the utilization of nano-silica in enhancing the mechanical properties of weak kaolin soils. Design mixes using different percentages of nano-silica were investigated in the range between 0.25–1.20% from the dry weight of the kaolin soil. Various chemical, physical, and mechanical properties of each mixture have been investigated. The obtained results indicated that nano-silica addition to such kaolin soils decreased the plasticity index and the maximum dry density while increasing the plastic limit, the Liquid limit, and the optimum moisture content. In different curing days of the tested mixtures, maximum dry density was decreased, while the optimum moisture content increased. The optimum value of added nano-silica was less than 1% of the soil dry weight. In the modified kaolin soil with 0.9% nano-silica, the plastic limit was increased by 29%, while the liquid limit decreased by 13% in comparison with the untreated sample. After 28 days of the cured sample, the unconfined compressive strength readings increased by almost 14% compared to its reading on day one. Also, the California bearing ratio results recorded significant enhancement with nano-silica additives in comparison with the untreated kaolin soil. After 28 curing days, the sonicated samples recorded enhancement in the unconfined compressive strength readings by more than 5% and 9% with the additive N-Si (0.3% and 0.9%), respectively, when compared with the unsonicated samples.Graphical

Microbial communities and high trophic level nematodes in protected argan soil show strong suppressive effect against Meloidogyne spp.

In many agroecosystems, soil suppressiveness to root knot nematodes (RKN) is of great interest in defining the biological agents controlling population density, especially with growing concerns about the environmental and human health impacts of chemicals. In this study, we evaluated the suppressiveness to Meloidogyne spp. in two land use soils: a protected argan soil from the Souss Massa National Park (SMNP), Morocco, and a conventional soil from an adjacent non-protected area. Using next-generation sequencing (NGS) technology, we characterized the fungal and bacterial communities in these soils for the first time. Nematodes belonging to different trophic guilds were also analyzed to further understand ecological factors that enable suppressive organisms to function and persist in the soil. The experiment was conducted in a greenhouse with tomato plants grown in untreated and autoclaved soils, each inoculated with 800 Meloidogyne infective juveniles (J2). We found that omnivore-predator nematode abundance, structure, and maturity indices were higher in the protected soil indicating the presence of a well-structured soil food web and a stable ecosystem. After sixty-seven days, RKN population density and gall index were reduced by 79.6 % and 81.5 % in the protected soil, respectively. 50 % autoclaved protected soil was also suppressive, but not 75 % and totally autoclaved soil. In contrast, conventional soil amplified the RKN population by 1319 %. More than 6770 bacterial and 558 fungal taxa were detected in this study, with Firmicutes, Actinobacteria, Mortierellales, Orbiliales, Agaricales, Diversisporales, and Pleosporales being consistently associated with RKN suppression. We found that fungal diversity was higher in SMNP-protected soil. In conclusion, soil protection enhances soil ecosystem resilience and that protected argan soils can serve as a valuable source of biological agents for the sustainable management of phytoparasitic nematodes.

Field assessment of Lake Erie dredged sediment for specialty crops cultivation

AbstractAnnually, approximately 1.5 million tonnes of sediment are dredged from federal navigational channels in Lake Erie. Recognizing the potential influence of Lake sediments on soil compaction, structure, water retention capacity, and aeration, this research assessed the agronomic performance of selected specialty crops under varying sediment ratios in an open‐field production system. The experimental design involved three sediment application rates: 0 tonne (100% farm soil), 0.7 tonne (90% farm soil and 10% sediment), and 7 tonnes per bed (100% sediment). Lettuces (Lactuca sativa L.) were harvested 35 days after planting, with assessments including fresh and dry weights of leaves root biomass and root length measurements. Radishes (Raphanus sativus L.) were evaluated for root length, leaf fresh weight, root fresh weight, and diameter. Tomatoes (Solanum lycopersicum L.) plants were monitored for plant height and stem diameter. Fruit harvest occurred at days 70 and 75 post‐transplant. Metrics such as total number of marketable fruits, total fruit weight, number of US grade‐1 fruits, and polar and equatorial diameters were recorded. The results revealed significant positive effects of the 7‐tonne sediment treatment on lettuce, including increased dry leaf and root biomass, root lengths, and fresh weight. Similarly, radishes exhibited enhanced weight and length when grown in beds with 7 tonnes of sediment. Tomatoes from the 7‐tonne sediment treatment displayed higher values in plant measurements and harvested fruits. Overall, the findings indicate that soils treated with Lake Erie sediment positively influence the development and production of lettuce, radishes, and tomatoes compared to untreated soils.