Untreated Sites Research Articles

Substratum Raking Can Restore Interstitial Habitat Quality in Swedish Freshwater Pearl Mussel Streams

Functional and oxygenated stream beds provide crucial habitat for multiple endangered stream taxa, including endangered freshwater mussels, fishes, and insect larvae. Stream bed restoration measures such as substrate raking are often applied to mitigate excess fine sediment introductions and stream bed colmation, yet such measures are controversial. In this study, we conducted a systematic experiment in which sites with stream bed raking and removal of macrophytes were monitored over two years and compared with before-treatment conditions and untreated reference sites in the Swedish Brånsån stream, which still contains a population of the endangered freshwater pearl mussel Margaritifera margaritifera. The stream bed restoration resulted in improved habitat quality, as evident from decreased substrate compaction, increased redox potential, and oxygen supply into the stream bed. In contrast to previous studies in Central European catchments with more intensive agricultural catchment uses, the effects of the restoration measure were much longer, extending over two years. Consequently, stream bed raking and macrophyte removal can be considered a useful and more long-lasting restoration measure than currently assumed, especially in streams where excess input of fine sediment has already been mitigated, where catchment land use is rather extensive, and where near-natural flow regimes still prevail.

Medium-term associations of soil properties and plant diversity in a semi-arid pine forest after post-wildfire management

The medium- and long-term studies about the effectiveness of post-fire management techniques on soil and plant ecology are scarce, although the effects of wildfire and subsequent management can be long lasting. This study has evaluated the changes in the main physico-chemical properties of soil and plant diversity six years after a wildfire and post-fire treatments using contour felled log debris (CFD) and log erosion barriers (LEB) in a Mediterranean pine forest in comparison to unburnt (UB), and burnt but untreated (BNA) sites. Soil texture and pH did not generally change after wildfire and treatments, while organic matter and nutrients were significantly different between the treated soils and the other sites. Herbaceous plants were found only in UB and LEB sites, the latter showing the lowest number of tree species. Shrubs were equally distributed among the four soil conditions. Resprouting and germinating plants increased in the treated sites compared to BNA area, which however showed a higher number of facultative resprouters. The analysis of relationships between soil properties and plant diversity showed that, when organic matter (CFD plots) and nitrogen (LEB plots) contents are noticeably higher compared to BNA soils, more resprouting species are detected. Moreover, significant increases in pH (UB and BNA plots) and organic matter (CFD plots) are associated to more abundant tree and herbaceous species. The study also indicates to forest managers the most resilient plant species after post-fire restoration several years after a wildfire under Mediterranean conditions.

Arthropod Recolonization of Soil Surface Habitat in Post-Fire Mulch Treatments

Increasing size, severity, and human proximity to fires in the western US are driving a need for more effective ecosystem restoration in the immediate post-fire period. Surface treatments, such as mastication of logging slash, reduce erosion and improve soil nutrient and water retention on steep slopes. However, few studies have investigated the impact of these treatments on arthropod communities over time. Our objective was to determine which insect communities return to these treated areas and if the mulch changes the community structure over time. We surveyed arthropod abundance using pitfall traps in mulch treatments in a landscape-scale fire near Flagstaff, Arizona, and a controlled split-plot experiment outside of the larger fire footprint. Predatory beetles were more abundant in mulch in the large landscape treatment, with no differences in abundance in the split plots. Fungivores had no significant mulch preference, and several native bark beetles were more abundant in the untreated sites. We found that the size of the fire footprint and distance to the intact forest matrix likely impact arthropod community composition over time. We were unable to fully evaluate vegetation recovery, but further work will allow us to understand how surface treatments impact the interaction of arthropods and vegetation.

Treatment Response to Diphenylcyclopropenone in Patients with Alopecia Totalis/Universalis.

Alopecia totalis (AT) and Alopecia universalis (AU) are forms of Alopecia areata (AA) which represent the strongest predictor of poor prognosis since spontaneous regrowth is <10%. Topical immunotherapy agent, diphenylcyclopropenone (DPCP) has shown clinical efficacy with limited side effects in severe forms of AA. However, its specific role in AT/AU characterized by complete hair loss over the scalp can help highlight the efficacy of the drug with fewer confounders. Data were collected from 18 patients diagnosed with AT/AU and treated with topical immunotherapy with DPCP as per protocol by Happle et al. Baseline Severity of Alopecia Tool (SALT) score and subclass was recorded. In the case of AU, baseline body hair loss score was also recorded. Patients were reassessed after 6 months of treatment in terms of change in SALT score and hair regrowth was assessed using the Global Assessment Score. The side effects during treatment were also assessed and recorded. Eighteen patients of whom eleven (61.1%) were diagnosed as AU and seven (38.9%) as AT were treated. The mean age was 21.6, with a male: female ratio of 3:2. The comorbidities noted were atopy in six (33.3%), atopy and hypothyroidism in one (5.5%), Down's syndrome in two (11.1%), and hypothyroidism alone in one (5.5%) patient. The mean duration of disease at the time of presentation was 3 years and all patients had remained refractory to various other modalities of treatment. All patients had a baseline SALT score of 100 corresponding to S5. After 6 months of treatment, 27.7% of patients did not show any response (SALT score S5), 16.6% had a score of S4, 11.1% had a score of S3, 11.1% had a score of S2, 22.2% had a score of S1, and 11.1% had a score of S0. On assessing improvement in body hair loss score, 36.3% of patients showed no improvement, 36.3% showed partial improvement, and 27.2% of patients showed complete body hair regrowth. About 55.5% of patients developed notable side effects that included severe local reactions, cervical lymphadenopathy, acne and pigmentation at the site of application as well as untreated sites. The AT/AU subtypes of AA, was amenable to treatment with contact immunotherapeutic agent DPCP with a >75% hair regrowth in 33.3% of patients. The castling phenomenon was seen in 63.6% of AU patients. The adverse effects noted were not severe enough to deter treatment.

Effect of high-volume insecticide spraying on sand fly vectors in household gardens in Spain.

This study investigated the efficacy of high-volume spraying with the adulticide α-cypermethrin alone and in combination with the larvicide diflubenzuron on the density of sand flies in gardens of three detached households in periurban areas in southeast Spain. Treatments were applied four times between June and August 2016, and four nearby sites, two households and two non-urbanized sites, were untreated controls. The number of sand flies collected between May and October 2016 using sticky interception and light attraction traps, was 4446 specimens. Species identified morphologically included Sergentomyia minuta (n = 2101; 48%), Phlebotomus perniciosus (n = 1922; 44%), Phlebotomus papatasi (n = 173; 4%), Phlebotomus sergenti (n = 161; 4%) and Phlebotomus ariasi (n = 36; 1%). Sand flies were detected in both treated and untreated sites. The proportion of positive sticky traps and the median (range) density of sand flies in positive traps were 61% traps and 7 (2-172) sand flies/m2 /day in untreated sites, and 43% traps and 4 (1-56) sand flies/m2 /day in treated sites (p < 0.05). Similarly, for light traps, it was 96% traps and 30 (3-168) flies/trap/day, and 83% traps and 3 (1-12) sand flies/trap/day, respectively (p < 0.05). However, sand fly density followed a comparable seasonal pattern in untreated and treated sites and did not consistently decrease following insecticide applications. These results were confirmed with mixed negative binomial modelling of sand fly density adjusted for time since application, month, environmental setting and site. The limited efficacy of the treatments, added to their cost, the impact of insecticides on non-target organisms and human health, and the risk of development of insecticide resistance, should dissuade similar outdoor applications to control sand fly vector populations in residential areas.

Woody species diversity and regeneration status of Sub-Alpine forest of Mount Adama exclosure site, Northwestern highlands of Ethiopia

Forests provide a wide range of ecosystem services. Despite these facts, the expansion of agriculture and settlement at the expense of forest resources has threatened the forest resources and results in biodiversity loss. To halt this problem, various conservation practices that believed to restore the degraded lands and biodiversity of the country have been implemented. Area exclosure is among the conservation strategies that have been used to restore the degraded lands in Mount Adama forest. However, its role in woody species regeneration in Mount Adama was not investigated. Thus, the objective of the study was to evaluate the impact of area exclosure on woody plant species composition, regeneration status, structure and diversity in Mount Adama. A systematic transect sampling method was used to collect vegetation data. Hence, 53 plots with 400 m2 area were laid along 11 transects. Then, within the main plots, five subplots with 1 m2 were laid to determine the abundance and frequency of seedlings. The results showed that about 31 woody species that belong to 30 genera and 19 families including four endemic species were identified. The majority (67.74%) of the species were categorized under shrub habitat, while the remaining 19.35% and 12.90% were trees and lianas or climbers, respectively. Asteraceae family was dominant by contributing 4 species followed by Rosaceae and Solanaceae each contributed 3 species. Hypericum revolutum was the dominant species with 53.38 important value index followed by Erica arborea and Hagenia abyssinica with 49.12 and 40.05, respectively. The overall Shannon- Wiener diversity index and Shannon evenness in the exclosure site were 2.6 and 0.73, respectively. Furthermore, the number of seedlings and saplings were higher in the exclosure than the untreated site. The results of the study evidently showed that area exclosure that implemented in Mount Adam successfully contributed to the biodiversity restoration. Therefore, further conservation efforts targeting species with low IVI values are needed for sustainable management and ecological recovery of the area.

Pitting fails to sustain increases in ecosystem structure in arid rangelands

Mechanized approaches to the rehabilitation of degraded rangeland have been extremely popular since the early 1930s but their effectiveness depends on climatic and environmental conditions (e.g. soil texture, pre‐treatment plant cover). We evaluated the effects of pitting, a popular rehabilitation technique based on the creation of elongated soil depressions (pits), on ecosystem structure from 14 trials conducted at 10 sites across an extensive area (~100,000 km2) of rangelands in eastern Australia. Averaged across all sites and rehabilitation times, pitting had a significant positive effect on the cover of perennial forbs and annuals, and suppressed bare ground compared with untreated sites. However, these treatment effects varied with different site conditions such as soil texture, and pre‐treatment rainfall and plant cover among individual sites. Pre‐treatment plant cover, and rainfall in the 3 months prior to measurements increased the effects of pitting on litter cover, and time since treatment increased the relative effect of pitting on annual plant cover. Negative effect of pitting on the cover of bare soil declined with increasing rainfall. Soil moisture was generally greater beneath pits than either control soils or soils between the pits (inter‐pits). Despite some positive effects on annual plant and litter cover, pitting effects were generally short lived, suggesting that a single pitting treatment may fail to achieve long‐term rehabilitation. Given that these field trials were conducted half a century ago under conditions of higher average rainfall, the prognosis for the success of pitting under current conditions of lower rainfall and higher temperature is generally poor.

Elucidating mechanisms of attenuated skin vasodilation during passive heat stress in persons with spinal cord injury

Objective Persons with spinal cord injury (SCI) are unable to efficiently dissipate heat via thermoregulatory vasodilation as efficiently as able-bodied persons during whole body passive heat stress (PHS). Skin blood flow (SkBF) is controlled by dual sympathetic vasomotor systems: noradrenergic vasoconstrictor (VC) nerves and cholinergic vasodilator (VD) nerves. Thus, impaired vasodilation could result from inappropriate increases in noradrenergic VC tone that compete with cholinergic vasodilation or diminished cholinergic tone. To address this issue, we used bretylium (BR) which selectively blocks neural release of norepinephrine, thereby reducing noradrenergic VC tone. If impaired vasodilation during PHS is due to inappropriate increase in VC tone, BR treatment will improve SkBF responses during PHS. Design Prospective interventional trial. Setting laboratory. Participants 22 veterans with SCI. Interventions Skin surface areas with previously defined intact vs. impaired thermoregulatory vasodilation were treated with BR iontophoresis with a nearby untreated site serving as control/CON. Participants underwent PHS until core temperature rose 1°C. Outcome Measures Laser doppler flowmeters measured SkBF over BR and CON sites in areas with impaired and intact thermoregulatory vasodilation. Cutaneous vascular conductance (CVC) was calculated for all sites. Peak-PHS CVC was normalized to baseline (BL): (CVC peak-PHS/CVC BL) to quantify SkBF change. Results CVC rise in BR sites was significantly less than CON sites in areas with intact (P = 0.03) and impaired (P = 0.04) thermoregulatory vasodilation. Conclusion Cutaneous blockade of neural release of noradrenergic neurotransmitters affecting vasoconstriction did not enhance thermoregulatory vasodilation during PHS in persons with SCI; rather BR attenuated the response. Cutaneous blockade of neural release of noradrenergic neurotransmitters affecting vasoconstriction did not restore cutaneous active vasodilation during PHS in persons with SCI.

Performance and safety effectiveness evaluation of mini-roundabouts in Michigan

This study evaluates the performance and safety effectiveness of mini-roundabouts. Crash, operational, and geometric data for 14 mini-roundabouts located in the state of Michigan were collected for both before and after installation time periods between 2007 and 2016. Three years of complete before and after installation crash data were analyzed utilizing the Before-and-After study with Comparison Group methodology. A total 28 untreated sites with similar characteristics to the 14 mini-roundabouts sites were used to account for changes in crashes unrelated to the treatment (mini-roundabout installation), evaluate the impacts of the treatment on the intersection, and develop the crash modification factors (CMFs). The results for overall safety effectiveness indicate a decrease in total crashes at signalized intersections when converted into mini-roundabouts, and an increase in total crashes at two-way stop-controlled (TWSC) and all-way stop-controlled (AWSC) intersections when converted into mini-roundabouts. The recommended CMFs for converting a TWSC, AWSC, and signalized intersection into a mini-roundabout are 1.039, 1.051, and 0.755 for total crashes, respectively.

Effects of wildfire, torrential rainfall and straw mulching on the physicochemical soil properties in a Mediterranean forest

With the effects of fire, weather and post-fire management on soil properties having been studied mostly individually, there is little understanding of the combined effects of wildfire, heavy storm and straw mulching. In this study, we evaluated the changes in soil properties following a high-severity fire, post-fire soil treatment using straw mulch, and a torrential storm in a forest stand in north-western Portugal. The main physicochemical properties of the soil were evaluated in different soil conditions: a) burnt and untreated, b) burnt and mulched areas, and c) unburnt sites on three survey dates 1) soon after wildfire, 2) after the rainstorm, and 3) one year after wildfire. We found that soil water repellency strongly increased ephemerally immediately after wildfire, decrease after the storm and disappeared after one year. Fire reduced the soil organic matter (by 50%, on average), total nitrogen (by 85–90%) and available phosphorous (by about 45%) at both the mulched and untreated sites, and this effect was not changed by the ensuing rainfall. In comparison to the unburnt sites, the pH increased in the burnt soils, and the electrical conductivity decreased. Finally, the dynamics of the major cations and minor elements were affected differently by the wildfire and rainfall under the different soil conditions. One year after the fire, the most notable changes compared to the unburnt soil were detected in magnesium, potassium and almost all the minor elements. Based on these findings, instructions were given to the land managers (aim to control soil hydrophobicity following wildfire, supply more soil organic matter to the soil to avoid a decline in soil fertility, and use alternative actions to straw mulching to control the soil chemistry) that were aimed at more effective post-fire management in severely burnt areas in Mediterranean forests.

Effects of rice husk biochar on rill detachment capacity in deforested hillslopes

Biochar addition to soil has been found to be a useful practice to control erosion. However, few studies have explored the effects of biochar produced from husk of rice on rill erosion, due to overland flow in deforested areas. This study has evaluated the rill detachment capacity (Dc) and erodibility (Kr) in soil samples treated with rice husk biochar in comparison to untreated sites (control), collected in deforested hillslopes of Northern Iran. Dc was measured in a lab-scale hydraulic flume at four-bed slopes (9.7, 14.3, 19.5, and 24.2%) and five water discharges (0.24, 0.36, 0.47, 0.59, and 0.69 L m−1 s−1). Moreover, organic carbon, aggregate stability and bulk density of soils were measured. Dc was lower (on average − 32%) in the treated soil compared to the control. Dc was not correlated to the other physico-chemical properties of soil. Kr, estimated by linear regressions between Dc and shear stress, was noticeably lower (−79%) in the treated soil compared to the control. These results evidence that rice husk biochar is effective at controlling and mitigating soil detachment, and at improving organic matter content and aggregate stability in deforested hillslopes. The proposed values of rill erodibility and shear stress of deforested hillslopes (treated or untreated) are helpful to model soil resistance to rill erosion in process-based erosion models.

Effective use of farmland soil samples for N and O isotopic source fingerprinting of groundwater nitrate contamination in the subsurface dammed limestone aquifer, Southern Okinawa Island, Japan

Nitrate contamination of groundwater due to improper management of fertilizer application and controls during agricultural activities is an escalating worldwide issue. Numerous studies have reported similar contamination in the Ryukyu limestone aquifer in the southern part of Okinawa Island. The study area has a unique setting where the subsurface dams hold the groundwater behind their cut-off walls, probably holding contaminated waters. This study provides updated insights on the source of contamination using stable N and O isotope ratios of dissolved nitrate (ẟ15NNO3 and ẟ18ONO3) in the aquifer waters. To propose a better reference in the source fingerprinting assessment, a special emphasis was placed on the isotopic comparison between the fertilizers and farmland soils where the respective fertilizers were applied. A total of 176 groundwater samples were collected from several spatial and monthly sampling campaigns for the purpose of source determination. Our isotopic comparison detected the enrichment of 15N of nitrate-nitrogen in the soil samples after the application of both chemical fertilizers and manure due to nitrification and volatilization. Unlike results from previous studies, the ẟ15NNO3 compositions of most groundwater samples did not fall into the range of soils mainly controlled by chemical fertilizers (from +3‰ to +5‰), but were comparable to the soils affected by mixed fertilizers including both chemical fertilizers and manure (from +7‰ to +9‰). The soils beneath untreated manure storage sites characterized by the highest ẟ15NNO3 values (from +18‰ to +20‰) affected some groundwater, which shows an even higher value (> +9‰). The contaminated waters tend to be gathered close to the subsurface dams and this tendency did not differ between two subsurface dam settings, Komesu and Giza. However, due to more impact from manure storage sites, the groundwater in the Komesu area tends to show slightly higher δ15NNO3 values than those in the Giza area. Our study demonstrated the usefulness of soil samples for precise isotopic source identification in farmland areas.

Evaluating Golden‐winged Warbler use of alder and aspen communities managed with shearing in the western Great Lakes

AbstractBest management practices are often written by researchers to guide land managers and landowners in the creation of habitat for wildlife species of interest. These documents are based on research evaluating the habitat needs of a species, but they also describe tools and strategies managers can implement to create or restore desired conditions. Shrub and sapling shearing is a management practice often used to improve habitat for early‐successional species, yet little monitoring or research has focused on wildlife response to shearing. The goal of this research was to formally evaluate the effect of shrub and sapling shearing as a best management strategy for Golden‐winged Warbler (Vermivora chrysoptera) conservation at a regional scale. Specifically, we surveyed for male Golden‐winged Warblers during the breeding season in sheared sites and untreated reference sites across portions of the western Great Lakes to assess the effects of (1) management status (i.e., sheared aspen or alder vs. untreated sites) and (2) patch‐level vegetation characteristics on male abundance. We found that male Golden‐winged Warbler abundance was twice as high in sheared sites than in mature reference sites and peaked when sapling cover was ~40%. Male abundance was also negatively associated with percent cover of forbs and nonvegetated ground. These findings highlight the importance of patch‐level heterogeneity when implementing shearing treatments for Golden‐winged Warblers and demonstrate the potential need for pretreatment site assessments to help focus conservation efforts for this species. Ultimately, our results support the use of a site‐specific, nuanced approach to shearing implementation to maximize cost efficiency and desired species outcomes.

Multi-year landscape-scale efficacy analysis of fluridone treatment of invasive submerged aquatic vegetation in the Sacramento–San Joaquin Delta

Invasive aquatic macrophytes are a major threat to estuarine ecosystems globally, posing difficult control challenges for resource managers. This study examined the efficacy of a fluridone treatment program to control invasive submerged aquatic vegetation (SAV). We leveraged four datasets to examine efficacy of fluridone treatment in the Sacramento–San Joaquin Delta, a freshwater tidal estuary in California, from 2014 to 2018. Annual SAV coverage maps derived from spectroscopy data were used in conjunction with fluridone application data and water current speed predicted by a 3-D hydrodynamic model of the study system. Our objective was to determine if the probability of SAV occurrence in treated sites was significantly different from untreated sites. We examined whether efficacy was influenced by the amount of herbicide applied at a site, the local current speed, and the treatment history of the site. We found that treatment efficacy was positively associated with the amount of herbicide applied per unit area, but this effect was mediated by a negative association with current speed. The efficacy of treatment did not improve when a site was treated for multiple consecutive years. Finally, we did not detect legacy effects one year after cessation of treatment. Our results suggest the need for careful selection of treatment sites to ensure that the amount of fluridone applied is likely to have significant impact given local current speeds. Our study underscores the need for development of additional treatment methodologies for hydrologically dynamic systems such as estuaries, particularly when control is needed year-round and for multiple years.

Effects of Post-Fire Mulching with Loranthus europaeus Jacq. on Surface Runoff and Rainsplash Erosion in a Semi-Arid Pine Forest

To avoid flooding and erosion hazards, post-fire management actions are essential in Mediterranean forests after severe wildfires. In this regard, mulching is the most common action but some mulch materials, such as straw, may lead to adverse impacts in burned forests. The use of yellow mistletoe fruits (Loranthus europaeus Jacq., hereafter “LE”) for the production of biodegradable mulch and its effectiveness in post-fire hydrology have never been studied. To fill this gap, this study has evaluated surface runoff and rainsplash erosion in a pine forest in Central Eastern Spain burned by a wildfire and mulched by a mixture of LE fruits and straw (with or without adding clay particles) using a portable rainfall simulator. Compared to untreated sites, runoff increased in burned and mulched soils (by 13.6% for the mixture without clay and by 17.2% when clay was added, in the latter case significantly). This increase was mainly due to the compact layer created by mulch application on the soil surface. However, the peak flow and the time to peak were lower in mulched soils (on average by 32.7% and 60.5%, significantly only for the mulch mixture without clay), thus indicating that, in these soils, peak runoff takes longer and its maximum value is lower compared to untreated sites. Soil erosion noticeably and significantly decreased (up to 97%) in mulched areas in comparison to untreated sites without significant differences between the two mixtures. Overall, this study indicates to land managers that soil mulching with a mixture of Loranthus europaeus Jacq. and straw is an effective post-fire management action to reduce the soil erosion risk after a wildfire.

Integrated Biological Control of the Sugar Beet Weevil Asproparthenis punctiventris with the Fungus Metarhizium brunneum: New Application Approaches.

The mass occurrence of the sugar beet weevil (Asproparthenis punctiventris, previously Bothynoderes punctiventris) has been endangering sugar beet cultivation in Austria for centuries. Exacerbated by climatic and political changes (warmer, drier spring and limited access to chemical pesticides), new approaches are needed to counter the problem. The aim of our work was to test whether the bioinsecticide Metarhizium brunneum Ma 43 (formerly M. anisopliae var. anisopliae BIPESCO 5/F52) can be used as a sustainable plant protection product against the sugar beet weevil. Our goal was to control the pest in all its development stages through multiple applications. Therefore, GranMetTM-P, a granular formulation of M. brunneum Ma 43, was applied in spring to establish the fungus in the soil, whereas GranMetTM-WP, a liquid formulation of the production strain, was used in early summer on trap ditches and leaves to target the adult weevils. Soil and plant samples as well as weevils were collected during the planting season from the trial sites to evaluate the development of the fungus and the mycosis of the treated weevils. In addition, data on hibernating weevils and their emigration from untreated field sites was collected. In all field sites, the Metarhizium spp. abundance increased above the background level (&lt;1000 CFU g-1 soil dry weight) after application of the product. With an increasing number of treatments per plot, and thus an increased contact possibility between pest and the fungus, a rise in the mycosis rate was observed. In conclusion, the various Metarhizium application strategies, which are already available or in testing, must be implemented to ensure control in both old and new sugar beet fields. Metarhizium is a further asset in the successful control of this sugar beet pest.

Soil mounding as a restoration approach of seismic lines in boreal peatlands: implications on microtopography

Seismic lines—narrow and straight corridors from which overstory has been removed to allow access for oil and gas exploration—are a major human footprint in the boreal forest of western Canada. With slow to minimal recovery of tree cover along these corridors, seismic lines have become a persistent landscape feature affecting connectivity and habitat quality in forested ecosystems, particularly in wetland areas. Soil mounding is a common ground preparation treatment widely applied along seismic lines, with the expectation that it will enhance tree seedling establishment and improve the return of tree cover to disturbed areas. However, much is still unknown about environmental responses following treatment application. In this study, we compared the ground microtopography in treated and untreated seismic lines, as well as the relative elevation between treated and untreated seismic lines with their adjacent treed peatland. The ground elevation in both treated and untreated sites was significantly lower on seismic lines relative to their adjacent treed peatland, with a greater elevation difference in treated areas. Likewise, ground microtopography was orders of magnitude higher along treated areas compared to the natural variation in the adjacent treed peatland. Given the important changes in relative elevation and topography following treatment application, our results suggest the potential for eventual treatment success may be more unpredictable than expected; this may have critical consequences for other ecological properties beyond the restoration goal of tree establishment and return of tree cover.

Assessment of Truck-Mounted Area-Wide S-methoprene Applications to Manage West Nile Virus Vector Species in the Suburbs of Chicago, IL, USA.

West Nile virus remains the leading cause of arboviral neuroinvasive disease in the United States, despite extensive efforts to control the mosquito vectors involved in transmission. In this study, we evaluated the effectiveness of Altosid SR-20 (active ingredient, S-methoprene 20%) larvicide applications using truck-mounted ultra-low volume (ULV) dispersal equipment to target Culex pipiens Linnaeus (Diptera: Culicidae) and Cx. restuans (Theobald)larvae. A combination of emergence bioassays, open-field measurements of deposited S-methoprene and spray distribution using gas chromatography-mass spectrometry, and assessments of adult Culex spp. populations in response to applications were conducted over the summer of 2020 within the North Shore Mosquito Abatement District (IL, USA). Open-field applications revealed that dispersed Altosid SR-20 using ULV equipment was effective (75% emergence inhibition in susceptible lab strain Cx. pipiens larvae) up to 53 m. In suburban neighborhood applications, we found that S-methoprene deposition and larval emergence inhibition (EI) in front yards did not differ significantly from backyards. An overall EI of 46% and 28% were observed for laboratory strain Cx. pipiens and wild Cx. restuans larvae respectively, and both had an EI significantly higher than the untreated control group. The EI of exposed wild Cx. pipiens larvae did not differ from the untreated controls, suggesting an increased tolerance to S-methoprene. No difference in abundance of gravid or host-seeking adult Culex spp. post-application was detected between treated and untreated sites. These results document the ability of area-wide application to distribute S-methoprene, but this strategy will need further modifications and evaluation for Culex spp. management.

The need for larval source management accompanying urban development projects in malaria endemic areas: a case study on Bioko Island

BackgroundIn 2017, several new housing districts were constructed on Bioko Island, Equatorial Guinea. This case study assessed the impact construction projects had on mosquito larval habitats and the effectiveness of larval source management in reducing malaria vector density within the surrounding area.MethodsAnopheline larval presence was assessed at 11 new construction sites by the proportion of larval habitats containing Anopheline pupae and late instar larval stages. Bacillus thuringiensis israelensis (Bti) larvicide was applied weekly to nine locations for 30 weeks, while two locations received no larvicide and acted as controls. Adult mosquito density was monitored via human landing collections in adjacent communities of six construction sites, including the two control sites.ResultsThe sites that received Bti had significantly lower observation rates of both pupae (3.2% vs. 18.0%; p < 0.001) and late instar Anopheles spp. mosquitoes (14.1 vs. 43.6%; p < 0.001) compared to the two untreated sites. Anopheles spp. accounted for 67% of mosquitoes collected with human landing collections and were captured at significantly lower levels in communities adjacent to treated construction sites compared to untreated sites (p < 0.001), with an estimated 38% reduction in human biting rate (IRR: 0.62, 95% CI IRR: 0.55, 0.69). Seven months after the start of the study, untreated sites were treated due to ethical concerns given results from treatment sties, necessitating immediate Bti application. The following week, the number of habitats, the proportion of larval sites with Anopheles spp. pupae, late instars, and adult biting rates in adjacent communities to these sites all decreased to comparable levels across all sites.ConclusionFindings suggest larval source management represents an effective intervention to suppress mosquito populations during infrastructure development. Incorporating larval source management into ongoing and planned construction initiatives represents an opportunity to fine tune vector control in response to anthropogenetic changes. Ideally, this should become standard practice in malaria-endemic regions in order to reduce viable mosquito habitats that are common by-products of construction.

Oligonychus perseae Tuttle, Baker & Abbatiello (Acari: Tetranychidae) population dynamics and associated phytoseiid mites (Acari: Phytoseiidae) in avocado orchards in the Canary Islands (Spain)

ABSTRACT The Persea mite, Oligonychus perseae, was identified for the first time in the Canary Islands (Spain) in 2006 becoming a major pest in avocado crops. This study analyses seasonal trends of Persea mite and phytoseiid predatory mites. The study aims to gain a better understanding of pest-predator dynamics and assess which predators have potential as biological control agents. In addition, predatory mites present in resident wild ground cover were studied with a view to promoting biological control. Results showed that Persea mite was present year-round with one or two peaks from April to July. The incidence of O. perseae varied depending on the avocado production area, but in all of them, it exceeded the density of 100 mites/leaf, established as the action threshold. Phytoseiids were more abundant on the trees in late winter, spring and early summer. Euseius stipulatus, Neoseiulus californicus and E. scutalis represented almost 96.5% of phytoseiids collected in commercial avocado orchards. They were also the most abundant species in the untreated study site. Resident wild ground cover associated with commercial avocado orchards was composed of 45 plant species belonging to 22 families, with Urticaceae and Asteraceae harbouring the highest richness in phytoseiid species.