Untreated Samples Research Articles

Effects of Sapindus mukorossi Seed Oil on Bone Healing Efficiency: An Animal Study.

Natural products have attracted great interest in the development of tissue engineering. Recent studies have demonstrated that unsaturated fatty acids found in natural plant seed oil may exhibit positive osteogenic effects; however, few in vivo studies have focused on the use of plant seed oil for bone regeneration. The aim of this study is to investigate the effects of seed oil found in Sapindus mukorossi (S. mukorossi) on the osteogenic differentiation of mesenchymal stem cells and bone growth in artificial bone defects in vivo. In this study, Wharton-jelly-derived mesenchymal stem cells (WJMSCs) were co-cultured with S. mukorossi seed oil. Cellular osteogenic capacity was assessed using Alizarin Red S staining. Real-time PCR was carried out to evaluate ALP and OCN gene expression. The potential of S. mukorossi seed oil to enhance bone growth was assessed using an animal model. Four 6 mm circular defects were prepared at the parietal bone of New Zealand white rabbits. The defects were filled with hydrogel and hydrogel-S. mukorossi seed oil, respectively. Quantitative analysis of micro-computed tomography (Micro-CT) and histological images was conducted to compare differences in osteogenesis between oil-treated and untreated samples. Although our results showed no significant differences in viability between WJMSCs treated with and without S. mukorossi seed oil, under osteogenic conditions, S. mukorossi seed oil facilitated an increase in mineralized nodule secretion and upregulated the expression of ALP and OCN genes in the cells (p < 0.05). In the animal study, both micro-CT and histological evaluations revealed that new bone formation in artificial bone defects treated with S. mukorossi seed oil were nearly doubled compared to control defects (p < 0.05) after 4 weeks of healing. Based on these findings, it is reasonable to suggest that S. mukorossi seed oil holds promise as a potential candidate for enhancing bone healing efficiency in bone tissue engineering.

Chemical improvement of soluble rocks: Foundation of Mosul Dam as case study

The dissolution of soluble rocks (gypsum/anhydrite) beneath the Mosul Dam by water seepage has been observed upon the initial impoundment; consequently, several sinkholes have been manifested in the vicinity of the dam site. Traditional grouting has been envisaged as a potential remedy; however this measure has not eradicated the problem. The main purpose of this study is to overcome the solubility of the gypsum/anhydrite rocks using chemical grouts. Rock samples were acquired from the Fatha Formation outcrop and problematic layers of brecciated gypsum situated at varying depths beneath the Mosul Dam. Two commercially available liquid polymers, polyurethane (PU) and a mixture of acrylic and cement (ARC) were used to investigate their sealing performance in halting of the solubility of the rocks (gypsum/anhydrite). To simulate the dissolution phenomenon under the influence of artificial hydraulic pressure of the dam and the water flow in its abutments, two distinct laboratory models were devised. The outcomes from the experimental study on both untreated and treated samples revealed that the acrylic-cement composite (ARC) and polyurethane (PU) are influential polymers in halting the solubility of the gypsum rock samples under both factors of water pressure and high-velocity water flow.

Comparative analyses of SARS-CoV-2 RNA concentrations in Detroit wastewater quantified with CDC N1, N2, and SC2 assays reveal optimal target for predicting COVID-19 cases

To monitor COVID-19 through wastewater surveillance, global researchers dedicated significant endeavors and resources to develop and implement diverse RT-qPCR or RT-ddPCR assays targeting different genes of SARS-CoV-2. Effective wastewater surveillance hinges on the appropriate selection of the most suitable assay, especially for resource-constrained regions where scant technical and socioeconomic resources restrict the options for testing with multiple assays. Further research is imperative to evaluate the existing assays through comprehensive comparative analyses. Such analyses are crucial for health agencies and wastewater surveillance practitioners in the selection of appropriate methods for monitoring COVID-19. In this study, untreated wastewater samples were collected weekly from the Detroit wastewater treatment plant, Michigan, USA, between January and December 2023. Polyethylene glycol precipitation (PEG) was applied to concentrate the samples followed by RNA extraction and RT-ddPCR. Three assays including N1, N2 (US CDC Real-Time Reverse Transcription PCR Panel for Detection of SARS-CoV-2), and SC2 assay (US CDC Influenza SARS-CoV-2 Multiplex Assay) were implemented to detect SARS-CoV-2 in wastewater. The limit of blank and limit of detection for the three assays were experimentally determined. SARS-CoV-2 RNA concentrations were evaluated and compared through three statistical approaches, including Pearson and Spearman's rank correlations, Dynamic Time Warping, and vector autoregressive models. N1 and N2 demonstrated the highest correlation and most similar time series patterns. Conversely, N2 and SC2 assay demonstrated the lowest correlation and least similar time series patterns. N2 was identified as the optimal target to predict COVID-19 cases. This study presents a rigorous effort in evaluating and comparing SARS-CoV-2 RNA concentrations quantified with N1, N2, and SC2 assays and their interrelations and correlations with clinical cases. This study provides valuable insights into identifying the optimal target for monitoring COVID-19 through wastewater surveillance.

Microwave-assisted hot air drying of Cannabis sativa: Effect of vacuum and pre-freezing on drying kinetics and quality

Drying is an important postharvest step preceding long term storage of medicinal plants, and optimized drying conditions are critical for secondary metabolite preservation. The objective of this research was to determine the effect of pre-freezing, vacuum, drying temperature, and microwave power on cannabis, including drying kinetics, color retention, and moisture diffusivity, and cannabinoid and terpene concentrations. Harvested inflorescence was dried under four drying conditions, namely untreated, dried without vacuum, untreated, dried with vacuum, pre-frozen, dried without vacuum, and pre-frozen, dried with vacuum. The untreated groups were dried and analysed immediately after harvest and the pre-frozen group was frozen at −20°C for 24 h prior to drying and analysis. Results showed that moisture diffusion was significantly affected by the temperature, vacuum and microwave power and reduced exponentially with time. For untreated samples dried at a microwave power of 2 W g−1, it took only 61 min (with no vacuum) and 57.3 min (under vacuum) at 65°C to reach the desired final moisture content of 11 % dry basis. Increasing drying temperature from 35°C to 50°C at 1 W g−1 under vacuum reduced the drying time by 60.1 % and 68 % for pre-frozen and untreated samples, respectively and reduced by 68 % and 65.5 % for pre-frozen and untreated cannabis samples, respectively for samples dried without vacuum. Pre-freezing prior to hot air drying with and without vacuum can be used to preserve the color of biomass and cannabinoids. The effect was prominent in the acidic cannabinoids where pre-freezing prior to hot air drying at 50°C without vacuum increased tetrahydrocannabinolic acid and cannabigerolic acid concentrations from 20.6 to 23.6 g 100 g dry matter−1 and 0.47–0.61 g 100 g dry matter−1. Results presented add important knowledge for the optimization of postharvest steps for cannabis.

Solvent bar microextraction based on using deep eutectic solvent and multivariate optimization for simultaneous determination of antidepressant and antiepileptic drugs in biological fluids

AbstractMonitoring medications in biological fluids is an essential aspect of patient care, particularly in cases of altered mental status where accurate diagnosis, effective treatment, and even forensic examinations are crucial. In this study, a new approach combining hydrophobic-deep-eutectic and solvent-bar-microextraction (HDE-SBME) followed by a high-performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the simultaneous determination of desipramine, clomipramine, as antidepressant and carbamazepine as antiepileptic agents in untreated human urine and plasma samples. The HDE solvents, synthesized using various ratios of menthol and fatty acids, were utilized in the SBME setups. Computational methods were employed to predict the structure and modes of interaction between HDE and the chosen analytes. Central composite design methodology (CCD) was used for multivariate optimization of the effects of different parameters influencing the extraction efficiency of the proposed method. Under optimized experimental conditions, the calibration graph of the spiked selected drugs in urine and plasma samples demonstrated excellent linearity (R2 ≥ 0.994), with limits of detection/quantification below 0.60/2.02 μg L−1. The extraction recoveries achieved were 88–97%, and the repeatability/reproducibility (RSD%, n = 5) was less than 6.12/7.57. The proposed method was successfully applied to determine selected drugs in patients' urine and plasma samples. The proposed method detects three drugs in patients' urine and plasma samples without using toxic volatile organic solvents. The proposed microextraction technique exhibited a confident sensitivity, feasible operation, and simplicity compared with other published methods. Thus, it can be considered a promising method for monitoring the therapeutic levels of specific antidepressant and antiepileptic drugs in urine and plasma samples.

Effect of Chemical Preservative and Packaging Material during Storage

Antinutritional properties of yam flour treated with chemical preservatives during six months of storage were carried out. Yam samples were purchased from the wurukum market, processed to obtain yam flour treated with chemical preservatives and packaged in plastic and low-density polyethene. About 100 grams of each of the different samples were separated into five portions. The first portion (sample A) was treated in a water bath with 250 ml of water with 0.5% of sodium metabisulphite for 15 minutes, drained and dried in an automated drier at about 70oC until dried to brittleness, Second portion (Sample B) was immersed in a solution of 0.5% Ascorbic acid for 15 minutes respectively, Third portion(Sample C) was immersed in a solution of 0.5% of Citric acid for 15 minutes, Fourth portion ( Sample D) was immersed in a solution of 0.5% of Ascorbic and Citric Acid, Fifth portion was blanched at 70oC for 5 minutes respectively. The yam slices were dried to brittleness and milled separately with a laboratory hammer mill and sieved using a 250-um mesh to obtain yam flour referred to as high–quality yam flour. The flour samples were analyzed for antinutritional using standard laboratory procedures. The anti-nutritional factors in the high‐quality yam flour were significantly different (p ≤ 0.05) from each other in terms of pretreatment but there was no significant difference in packaging material across storage. Antinutritional factors of yam flour samples decreased as storage progressed. The alkaloid contents of the different yam flour samples ranged from 0.17mg/100 g before storage to 0.39mg/100 g two months after storage, The Tannin contents of the different yam flour samples ranged from 0.32–0.68 mg/100 g (month 0), 0.04–0.64 mg/100 g (month 2), 0.31–0.58 mg/100 g (month 4), and 0.28–0.52mg/100 g (month 6) and The saponnin contents of the different yam flour samples ranged from 0.21–0.39 mg/100 g (month 0), 0.20–0.34 mg/100 g (month 2), 0.19–0.35 mg/100 g (month 4), and 0.21–0.32 mg/100 g (month 6). The anti-nutritional factors in the treated and untreated yam flour samples were significantly (p &lt; .05) affected by pretreatment, storage and packaging materials. Chemical preservatives used in yam processing makes treated yam flour safe for consumption after six months of storage.

Integrated analysis of ferroptosis and stemness based on single-cell and bulk RNA-sequencing data provide insights into the prognosis and treatment of esophageal carcinoma

BackgroundCancer stem cells (CSCs) play a significant role in the recurrence and drug resistance of esophageal carcinoma (ESCA). Ferroptosis is a promising anticancer therapeutic strategy that effectively targets CSCs exhibiting high tumorigenicity and treatment resistance. However, there is a lack of research on the combined role of ferroptosis-related genes (FRGs) and stemness signature in the prognosis of ESCA. MethodsThe cellular compositions were characterized using single-cell RNA sequencing (scRNA-seq) data from 18 untreated ESCA samples. 50 ferroptosis-related stemness genes (FRSGs) were identified by integrating FRGs with stemness-related genes (SRGs), and then the cells were grouped by AUCell analysis. Next, functional enrichment, intercellular communication, and trajectory analyses were performed to characterize the different groups of cells. Subsequently, the stem-ferr-index was calculated using machine learning algorithms based on the expression profiles of the identified risk genes. Additionally, therapeutic drugs were predicted by analyzing the GDSC2 database. Finally, the expression and functional roles of the identified marker genes were validated through in vitro experiments. ResultsThe analysis of scRNA-seq data demonstrates the diversity and cellular heterogeneity of ESCA. Then, we identified 50 FRSGs and classified cells into high or low ferroptosis score stemness cells accordingly. Functional enrichment analysis conducted on the differentially up-regulated genes between these groups revealed predominant enrichment in pathways associated with intercellular communication and cell differentiation. Subsequently, we identified 9 risk genes and developed a prognostic signature, termed stem_ferr_index, based on these identified risk genes. We found that the stem-ferr-index was correlated with the clinical characteristics of patients, and patients with high stem-ferr-index had poor prognosis. Furthermore, we identified four drugs (Navitoclax, Foretinib, Axitinib, and Talazoparib) with potential efficacy targeting patients with a high stem_ferr_index. Additionally, we delineated two marker genes (STMN1 and SLC2A1). Particularly noteworthy, SLC2A1 exhibited elevated expression levels in ESCA tissues and cells. We provided evidence suggesting that SLC2A1 could influence the migration, invasion, and stemness of ESCA cells, and it was associated with sensitivity to Foretinib. ConclusionThis study constructed a novel ferroptosis-related stemness signature, identified two marker genes for ESCA, and provided valuable insights for developing more effective therapeutic targets targeting ESCA CSCs in the future.

Fabrication of gradient structure for enhancing wear resistance of GCr15 bearing steel friction shim

The wear resistance of metallic shims under heavy load is crucial for the friction behavior of friction damper in civil engineering. This study verifies the potential of the strengthening grinding process (SGP) to enhance the wear resistance of metallic materials under heavy load. A multidisciplinary effort is shown in this paper to investigate the wear mechanism of SGP-treated GCr15 bearing steel in terms of the application scenario of civil engineering. The SGP treatment uses a multiphase abrasive comprising steel balls, corundum powder, and strengthening liquid to impact the surface of the GCr15 bearing steel. Then, a dry friction sliding test was performed on the polished, sandblasted, and SGP-treated GCr15 bearing steel. The microtopography and microstructure of these three friction shims with different treatments before and after the wear test were compared and analysed. The test results indicated the feasibility of SGP in enhancing the wear resistance of GCr15 bearing steel friction shims under heavy loads in terms of the application scenario of civil engineering. The SGP-treated sample had a more stable friction coefficient (0.475) and slighter wear compared to the other two samples due to its enhanced layer with a gradient structure and a thickness of approximately 23 μm.

The Development of Sustainable Biocomposite Materials Based on Poly(lactic acid) and Silverskin, a Coffee Industry By-Product, for Food Packaging Applications

Aligned with the principles of the circular economy and aiming at the production of environmentally friendly materials for food packaging applications, sustainable biocomposite films based on poly(lactic acid) (PLA) and coffee silverskin (SS), were developed. Coffee silverskin is a by-product of the coffee roasting process, while PLA is one of the most promising bio-based polymers. Several composites were prepared with different loadings of SS, ranging from 2.5 to 20 wt.%, via the solution casting method. The findings indicated that the effective dispersion of coffee silverskin in PLA was successfully accomplished and that a bleaching treatment of the filler leads to better interfacial interaction. The addition of silverskin, in any proportion, did not affect the melting point and glass transition temperature of the polymer matrix or the oxygen permeability of the film. Moreover, the degree of swelling was increased, more so for the films with modified particles, whereas the water vapor transmission rate and permeability increased only after the addition of high amounts (&gt;10%) of surface-treated silverskin. A gradual decrease in color lightness was measured with the increasing concentration of silverskin, and the color was more intense in the untreated samples. The antioxidant activity of the films increased gradually with increasing additions of coffee silverskin due to the presence of compounds such as polyphenols. The chemical treatment of coffee silverskin resulted in the films having improved mechanical properties, as the chemical treatment facilitated stronger bonding between the base material and the additive. Therefore, sustainable composites with enhanced antioxidant activity can be produced by the incorporation of a food industry by-product into a PLA matrix.

Effect of corrosion on the fatigue damage behavior of M50 steel treated by ultrasonic surface rolling process

Ultrasonic surface rolling process (USRP) can improve the fatigue performance of M50 steel, but corrosion during service may affect the anti-fatigue performance. Microstructure and corrosion resistance of M50 steel treated by USRP is analyzed by field-emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) and electrochemical impedance spectroscopy (EIS). Besides, the fatigue behavior of USRP sample under corrosion is analyzed by conducting fatigue tests after immersion corrosion tests. Results show that USRP treatment refines the surface grains to nanoscale (29.8 nm), introduces a certain residual compressive stress and decrease the precipitations of M50 steel, which improve the density of passive film and the resistance to local corrosion for M50 steel. Compared to M50 steel matrix, USRP sample has fewer and smaller corrosion pits at the same corrosion time, thereby reducing the risk of crack initiation. Meanwhile, the residual compressive stress value of USRP sample remains at a higher level. So the fatigue life of USRP sample is always higher than that of untreated sample under the same corrosion time. However, the corrosion process still leads to the destruction of surface integrity and the release of residual compressive stress, and the fatigue performance of USRP sample will gradually decrease with increasing the corrosion time.

Steam Explosion-Assisted Extraction of Ergosterol and Polysaccharides from Flammulina velutipes (Golden Needle Mushroom) Root Waste.

In this work, steam explosion (SE) was applied to prompt the rapid extraction of ergosterol and polysaccharides from Flammulina velutipes root (FVR) waste. Ultrasound-assisted saponification extraction (UASE) followed by water extraction was used to prepare ergosterol and polysaccharides. The results indicated that SE destroyed the complicated structure of FVR and increased its internal porosity and surface roughness. SE caused the thermal degradation of FVR's structural components and increased the polysaccharide content 0.97-fold. As a result, the extraction yield and efficiency of ergosterol and polysaccharides were improved. The theoretical maximum extraction concentration (C∞) and diffusion coefficient (D) were increased by 34.10% and 78.04% (ergosterol) and 27.69% and 48.67% (polysaccharides), respectively. The extraction yields obtained within 20-30 min of extraction time exceeded those of untreated samples extracted after several hours. For polysaccharides, SE led to a significant reduction in the average molecular weight, increased the percentage of uronic acids and decreased the neutral sugar percentage. The monosaccharide composition was changed by SE, with an increase in the molar ratio of glucose of 64.06% and some reductions in those of other monosaccharides. This work provides an effective method for the processing of fungi waste and adds to its economic value, supporting its high-value utilization in healthcare products.

Synergistic Effects of Combined Flavourzyme and Floating Electrode-Dielectric Barrier Discharge Plasma on Reduction of Escherichia coli Biofilms in Squid (Todarodes pacificus).

This study investigated the synergistic effect of combining flavourzyme, a natural enzyme, and floating electrode-dielectric barrier discharge (FE-DBD) plasma (1.1 kV, 43 kHz, N2 1.5 m/s) treatment, a non-thermal decontamination technology, against Escherichia coli biofilms in squid. E. coli (ATCC 35150 and ATCC 14301) biofilms were formed on the surface of squid and treated with different minimum inhibitory concentrations (MICs) of flavourzyme (1/8; 31.25 μL/mL, 1/4; 62.5 μL/mL, 2/4; 125 μL/mL, and 3/4 MIC; 250 μL/mL) and FE-DBD plasma (5, 10, 30, and 60 min). Independently, flavourzyme and FE-DBD plasma treatment decreased by 0.26-1.71 and 0.19-1.03 log CFU/cm2, respectively. The most effective synergistic combination against E. coli biofilms was observed at 3/4 MIC flavourzyme + 60 min FE-DBD plasma exposure, resulting in a reduction of 1.55 log CFU/cm2. Furthermore, the combined treatment exhibited higher efficacy in E. coli biofilm inactivation in squid compared to individual treatments. The pH values of the synergistic combinations were not significantly different from those of the untreated samples. The outcomes indicate that the combined treatment with flavourzyme and FE-DBD plasma can effectively provide effective control of E. coli biofilms without causing pH changes in squid. Therefore, our study suggests a new microbial control method for microbial safety in the seafood industry.

Fungal degradation of bamboo treated with crude lake salt and a mixture of borax and boric acid

This study compared the effectiveness of crude lake salt, a traditional preservative used by artisans in Uganda, and borax-boric acid, a conventional preservative, against fungal degradation. Using the European standard, an experiment was set up to determine the durability of Oxytenanthera abyssinica, Oldeania alpina and Bambusa vulgaris treated with 2 % and 6 % crude lake salt and borax-boric acid against Gloeophyllum trabeum, Coniophora puteana and Trametes versicolor. Mass loss comparisons between treated and untreated bamboo samples were made. Durability classes were assigned according to the amount of mass lost. Bamboo samples exposed to G. trabeum had lower surface hyphal coverage compared to those exposed to C. puteana and T. versicolor irrespective of the preservative used. Samples treated with borax-boric acid were in durability class I compared to those treated with crude lake salt that were between class II and III according to European standard. The lowest mass loss for bamboo treated with crude lake salt was 5.9 % in O. abyssinica exposed to G. trabeum. The highest mass loss for bamboo treated with crude lake salt was 14.24 % in B. vulgaris exposed to T. Versicolor. The study confirmed that while artisans use crude lake salt as a traditional preservative, it does not protect against white and brown rot while all concentrations of borax-boric acid provided protection against all fungi.

Enhancing performance of Prosopis juliflora fiber reinforced epoxy composites with silane treatment and Syzygium cumini filler

The utilization of natural fibers and fillers in composite materials has gained significant attention for their potential to enhance mechanical, thermal, and tribological properties while promoting sustainability. In this study, Prosopis juliflora fiber (PJF) reinforced epoxy composites, treated with silane and incorporating Syzygium cumini filler (SCF), were investigated to assess their performance across various parameters. The composites were subjected to comprehensive mechanical, tribological, thermo-gravimetric, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), 3D profilometer, and morphology studies to elucidate the effects of silane treatment and SCF content on composite properties. The results of the mechanical characterization revealed significant improvements in tensile strength, impact strength, and microhardness in silane-treated composites with higher SCF content compared to untreated samples and those with lower SCF content. Specifically, the ST/SCF3 composite exhibited the highest values, with a tensile strength of 118.65 ± 4.63 MPa, impact strength of 27.83 ± 1.96 kJ/m2, and microhardness of 91.63 ± 2.84 HV. Tribological analysis indicated enhanced wear resistance and reduced coefficient of friction in silane-treated composites with higher SCF content, with the ST/SCF3 composite demonstrating the lowest wear loss (62 μm) and frictional force (5.2 N). Thermal characterization revealed improved thermal stability in silane-treated composites, particularly in the ST/SCF1 and ST/SCF3 formulations, suggesting increased resistance to temperature-induced degradation.

Effect of Application of a Cactus Pear Mucilage-Based Edible Coating Enriched with Glycerol and L-Glutamine on Minimally Processed White-Flesh Loquats

Loquat (Eriobotrya japonica Lindl.), a non-climacteric fruit, is susceptible to physical and mechanical damage, as well as decay, especially after minimal processing, resulting in a short postharvest lifespan The objective of our study was to evaluate the impact of a cactus pear (OFI) mucilage-based edible coating enriched with glycerol and l-glutamine on the quality and nutraceutical value of minimally processed white-flesh Martorana loquat fruits during cold storage. After washing and processing the cladodes, mucilage was extracted, and two different coatings (EC1: 60% OFI mucilage, 40% glycerol; EC2: 67% OFI mucilage, 30% glycerol, 3% glutamine) were formulated and compared with an untreated sample (CTR). Our analyses covered various parameters, including color, total soluble solid content, titratable acidity, antioxidant activity, and total phenols. Additionally, sensory analysis was conducted and visual scores were obtained. The results suggest that the application of a cactus pear mucilage-based edible coating, supplemented with glycerol and L-glutamine, effectively preserves the quality attributes of minimally processed loquat fruits.

Comparative study on the fretting and sliding wear properties of micro-arc oxidation-treated aluminum alloy at different temperatures

Micro-arc oxidation is an effective method to enhance surface wear resistance. This paper comparatively studies the fretting and sliding wear properties of micro-arc oxidation-treated 7075 aluminum alloy at different temperatures. Research indicates that both the untreated and MAO-coated samples exhibit peak friction coefficient values due to adhesion at elevated temperatures. At both room temperature and 150 °C, the fretting wear rate of the MAO-coated sample is lower than that of the sliding wear rate, which can be attributed to distinct surface damage mechanisms and third body behavior under different wear modes. Adhesive wear is more prominent in MAO-coated samples during high-temperature fretting compared to room temperature, while abrasive wear and spalling are more prevalent during high-temperature sliding.

Dynamic response, durability, and carbon footprint analysis of the marl clay treated with sodium lignosulfonate as a sustainable-environmentally friendly approach

In the current study, the marl clay was improved by different contents of sodium lignosulfonate (NLS) and cured at different times and then, all samples were subjected to Bender Element (BE), Unconfined Compressive Strength (UCS), and Brazilian Tensile Strength (BTS) tests considering two different dry condition (DC) and wet condition (WC). The durability of the samples was further controlled by a special technique, namely the soaking test. The carbon footprint analysis was undertaken for a low-volume trench project to address the sustainability benefits associated with replacing cement and lime as traditional stabilizers with NLS. The results show that the reuse of NLS as a non-traditional alternative for stabilizing marl soil can play an influential role in improving dynamic parameters as well as sustainable development. It has been observed that the CO2 emission decreases up to 5.6 and 4.4 times compared to lime and cement, respectively. Additionally, the use of NLS enhances the UCS by 249%, BTS by 208%, and small strain shear modulus by 117%. Furthermore, reducing the adverse effects of the WC on soil properties, among others, was the main finding of utilizing the NLS in marl stabilization with curing time. NLS-treated marl samples were able to preserve the integrity of their particles even after being soaked in water for a period of 3 weeks. In contrast, the particles of the untreated sample started to disintegrate within a few seconds of initiating the soaking test. Finally, possible equations correlating the dynamic and static moduli were reported in this study.

Integration of pre-treatment computational radiomics, deep radiomics, and transcriptomics enhances soft-tissue sarcoma patient prognosis

Our objective was to capture subgroups of soft-tissue sarcoma (STS) using handcraft and deep radiomics approaches to understand their relationship with histopathology, gene-expression profiles, and metastatic relapse-free survival (MFS). We included all consecutive adults with newly diagnosed locally advanced STS (N = 225, 120 men, median age: 62 years) managed at our sarcoma reference center between 2008 and 2020, with contrast-enhanced baseline MRI. After MRI postprocessing, segmentation, and reproducibility assessment, 175 handcrafted radiomics features (h-RFs) were calculated. Convolutional autoencoder neural network (CAE) and half-supervised CAE (HSCAE) were trained in repeated cross-validation on representative contrast-enhanced slices to extract 1024 deep radiomics features (d-RFs). Gene-expression levels were calculated following RNA sequencing (RNAseq) of 110 untreated samples from the same cohort. Unsupervised classifications based on h-RFs, CAE, HSCAE, and RNAseq were built. The h-RFs, CAE, and HSCAE grouping were not associated with the transcriptomics groups but with prognostic radiological features known to correlate with lower survivals and higher grade and SARCULATOR groups (a validated prognostic clinical-histological nomogram). HSCAE and h-RF groups were also associated with MFS in multivariable Cox regressions. Combining HSCAE and transcriptomics groups significantly improved the prognostic performances compared to each group alone, according to the concordance index. The combined radiomic-transcriptomic group with worse MFS was characterized by the up-regulation of 707 genes and 292 genesets related to inflammation, hypoxia, apoptosis, and cell differentiation. Overall, subgroups of STS identified on pre-treatment MRI using handcrafted and deep radiomics were associated with meaningful clinical, histological, and radiological characteristics, and could strengthen the prognostic value of transcriptomics signatures.

Optimizing color enhancement in dried Penaeus vannamei through high-humidity hot air impingement cooking: Enzyme inactivation, reduced drying time, and Maillard reaction inhibition

This study was aimed to evaluate the potential of high-humidity hot air impingement cooking (HHAIC) on Penaeus vannamei, focusing on its drying characteristics, microstructure, water distribution, enzyme activity, astaxanthin content, antioxidant capacity, color, and Maillard reaction. Results demonstrated that a 3 min HHAIC significantly improved the shrimp's color and optimized astaxanthin content with a notable increase in scavenging capacity based on an in-vitro as antioxidation activity evaluation. Compared to the untreated samples, HHAIC could significantly inactivate polyphenol oxidase by 95.76%. Also, it suppressed the Maillard reaction by decreasing 5-hydroxymethylfurfural content and shortened the drying time by 40%. In addition, the low-field nuclear magnetic resonance and microstructure analysis showed alterations in the shrimp muscle fiber structure and water distribution. This study indicated that HHAIC could elevate quality, enhance appearance, and reduce the processing time of dried shrimp, presenting valuable implications for industry progress.

Surface Growth of Boronize Coatings Studied with Mathematical Models of Diffusion

The following investigation focused on examining the kinetics of Fe2B coating formation on the surface of ASTM A681 steel during the powder-pack boronizing process. The study measured Fe2B coating thicknesses at various temperatures and exposure times to confirm the diffusion-controlled growth mechanism during boronizing. Five distinct mathematical models were devised to determine the boron diffusion coefficients in Fe2B coatings. Understanding the growth kinetics of boronize coatings is imperative as it facilitates the optimization and automation of industrial processes. This ensures the efficient and consistent production of boronize coatings on cutting tools, such as drills and milling cutters, due to their high hardness and wear resistance. The value of the activation energy estimated with five mathematical diffusion models for the Fe2B coating was 209.8 kJ∙mol−1. The X-ray diffraction technique was used to identify the presence of the iron boronize phase. Tribological studies were also performed to evaluate the coefficient of friction (COF) of the boronized (0.256) and untreated (0.781) samples, having a 300% positive effect of the boronize coating on wear resistance. Finally, the models were empirically validated for two supplementary treatment conditions for 1223 K for 3 h and 1273 K for 1.5 h, where the percentage error for both conditions was estimated to be approximately 2.5%.