Untreated Areas Research Articles

Phytoindication assessment of the effect of reconstruction on the light regime of an urban park

The ecological restoration of urban parks is used to increase their recreational attractiveness, improve air quality, mitigate urban heat island effects, improve stormwater infiltration, and provide other social and environmental benefits. The dynamics of plant communities after urban forest restoration requires investigation. The study assessed the impact of urban park reconstruction on the state of grass cover, phytoindication of changes in light regime caused by park reconstruction and found out the dependence of reliability of phytoindication assessment on the number of species in the relevant area. The study was conducted in the recreational area of the Botanical Garden of the Oles Honchar Dnipro National University (Ukraine). A tree plantation was created after the Second World War in the location of a natural oak forest. In 2019, a 2.8 ha area of the park was reconstructed. The samples were taken within polygons, two of which were placed in the reconstruction area and two of which were placed in a similar section of the park where no reconstruction was performed. During the reconstruction process, walkways were rebuilt, shrubs were removed, old, damaged trees were removed, and tree crowns were trimmed. Juvenile trees were planted in place of the removed old trees. Old outbuildings, which greatly impaired the aesthetic perception of the park, were also removed. Transport and construction machinery was involved in the reconstruction. A total of 65 plant species were found within the studied polygons. The number of herbaceous species in the park area after reconstruction was higher than without reconstruction. The crown closure in the reconstructed area was significantly lower than that in the untreated conditions. The phytoindication assessment showed that the light regime varies from the conditions suitable for the scyophytes (plants of typical foliage forests) to the conditions suitable for the sub-heliophytes (plants of light forests and shrubberies, or high herbaceous communities; lower layers are in the shade). The light regime in the park area after reconstruction was statistically significantly different from the regime in the untreated park area. The lighting regime after the reconstruction was favourable to sub-heliophytes, and without reconstruction the regime favoured hemi-scyophytes. Tree canopy crown closure negatively correlated with grass height and herbaceous layer projective cover. The tree canopy crown closure, grass height, and herbaceous layer projective cover were able to explain 86% of the phytoindication assessment of the lighting regime variation. These parameters negatively affected the light regime. The prospect of further research is to investigate the dependence of indicative reliability of the assessment of other environmental factors with the help of phytoindication depending on the number of species. In addition to the indication of traditional ecological factors it is of particular interest to clarify the aspect of the dynamics of hemeroby indicators as a result of park reconstruction.

Short-Term Changes in Erosion Dynamics and Quality of Soils Affected by a Wildfire and Mulched with Straw in a Mediterranean Forest

Wildfire heavily impacts the quality of forest soils, and the precipitation occurring immediately after fire can determine high runoff and erosion rates, which may lead to noticeable soil degradation. Mulching is commonly used to limit the hydrological impacts of wildfire and climate, but this post-fire management technique may alter the erosion–deposition dynamics at the hillslope scale and, consequently, alter soil quality. In order to explore the magnitude and significance of these changes (little was studied in the literature until now), this communication reports the first results of a field activity that evaluated the changes in soil quality in areas affected by a wildfire and subjected to different post-fire treatments in Mediterranean forests. The main properties of sediments eroded from burned and untreated soils, and mulched soils (using a straw dose of 0.2 kg/m2 of dry weight), were measured after the first rainstorm (height of 37 mm and maximum intensity of 11.6 mm h−1) occurring two months after a wildfire (occurred on 30 June 2016) in a pine forest of Castilla-La Mancha (Spain). This event produced a runoff volume of 0.07 ± 0.02 mm in mulched soils and 0.10 ± 0.10 mm in non-mulched soils; soil loss was 0.20 ± 0.06 g/m2 in the mulched area and 0.60 ± 0.60 g/m2 in the non-mulched area. In comparison to burned and non-treated areas, this study showed: (i) increases in salinity, and reductions in organic matter, nutrients, nitrates, and micro-elements in burned and untreated soils; (ii) reductions in runoff (−20%) and in soil erosion (−60%) as a result of mulch cover; (iii) effectiveness of mulching in limiting the declines in soil quality detected in burned and eroded areas; and (iv) transport of low amounts (less than 10–15%) of some compounds (organic matter and nutrients) downstream of the fire-affected areas (both mulched and untreated). Phosphorous runoff toward valley areas and nitrate incorporation into the soil, detected in both mulched and untreated areas, require attention, since these processes may cause eutrophication of water bodies or nitrate pollution in groundwater.

Shrub encroachment, landscape restoration, and intraguild predation

Shrub encroachment into arid grasslands occurs globally with the potential to affect vertebrates and their interactions. In the Chihuahuan Desert of southern New Mexico, shrub encroachment has prompted intensive efforts by land management agencies to remove shrubs and restore historical grassland habitats. We asked if restoration actions involving shrub removal affected dynamics of intraguild predation (IGP) including an IGP predator (coyote, Canis latrans), an IGP prey (kit fox, Vulpes macrotis), plus their shared lagomorph prey. We used camera traps on 14 sites with paired treated and untreated areas to examine spatial and temporal niche partitioning of coyotes and kit foxes. Shrub removal did not produce straightforward effects on abundances of coyotes, kit foxes, or their prey resources. Instead, abundances of kit foxes were constrained when coyote abundance reached a threshold. Below this threshold, kit foxes were more common on areas with low shrub cover, possibly due to lack of hiding cover for lagomorph prey that increased their predation risk. Our system included two alternative states: IGP predator dominated and coexistence of IGP predator and prey. Coexistence may have been facilitated by temporal niche partitioning as diel activity patterns differed for coyotes and kit foxes. Future research on intraguild predation should integrate spatial and temporal niches to understand species coexistence including on dynamic landscapes undergoing restoration.

Sterile Insect Technique: Successful Suppression of an Aedes aegypti Field Population in Cuba.

Simple SummaryThe sterile insect technique (SIT) is a species-specific and environment-friendly method of insect control that relies on the release of large numbers of sterile insects. Mating released sterile males with wild females leads to a decrease in the reproductive potential and to the local suppression of the target population. There is increased interest in applying this approach to manage disease-transmitting mosquito populations. The main focus of this pilot trial was to assess the efficacy of the SIT for the suppression of Aedes aegypti populations. Two areas in Havana city, Cuba, were selected as control and release trial sites. The presence, density and fertility of the target wild population were monitored through a network of ovitraps. Approximately 1,270,000 irradiated Ae. aegypti males were released in the 50 ha target area over a period of 20 weeks. The released mosquitoes showed excellent mating competitiveness and induced high levels of sterility in the wild Ae. aegypti population. The target natural population was suppressed as reflected in the ovitrap index and in the mean number of eggs/trap values which dropped to zero by the last 3 weeks of the trial. We conclude that the released sterile male Ae. aegypti competed successfully and induced significant sterility in the local target Ae. aegypti population, resulting in suppression of the vector.Dengue virus infections are a serious public health problem worldwide. Aedes aegypti is the primary vector of dengue in Cuba. As there is no vaccine or specific treatment, the control efforts are directed to the reduction of mosquito populations. The indiscriminate use of insecticides can lead to adverse effects on ecosystems, including human health. The sterile insect technique is a species-specific and environment-friendly method of insect population control based on the release of large numbers of sterile insects, ideally males only. The success of this technique for the sustainable management of agricultural pests has encouraged its evaluation for the population suppression of mosquito vector species. Here, we describe an open field trial to evaluate the effect of the release of irradiated male Ae. aegypti on a wild population. The pilot trial was carried out in a suburb of Havana and compared the mosquito population density before and after the intervention, in both untreated control and release areas. The wild population was monitored by an ovitrap network, recording frequency and density of eggs as well as their hatch rate. A significant amount of sterility was induced in the field population of the release area, as compared with the untreated control area. The ovitrap index and the mean number of eggs/trap declined dramatically after 12 and 5 weeks of releases, respectively. For the last 3 weeks, no eggs were collected in the treatment area, clearly indicating a significant suppression of the wild target population. We conclude that the sterile males released competed successfully and induced enough sterility to suppress the local Ae. aegypti population.

Molecular and Histological Evidence Detailing Clinically Observed Skin Improvement Following Cryolipolysis.

BackgroundIn addition to body contouring, there is anecdotal and clinical evidence of reduced laxity caused by skin tightening after cryolipolysis. However, it has not been established how cryolipolysis triggers dermal changes. ObjectivesThe aim of this study was to investigate the fundamental mechanisms behind clinically observed dermal changes by molecular and immunohistochemistry (IHC) analytical methods.MethodsThis feasibility study involved 7 subjects who received cryolipolysis treatment. Tissue samples were harvested from 3 days to 5 weeks after treatment. RNA-sequencing examined differential gene expression of major collagens. RNA in situ hybridization (RNA-ISH) investigated the distribution of 1 of the gene markers for collagen type I (COL1A1). IHC for procollagen type I, heat shock protein 47 (HSP47), transforming growth factor β (TGF-β), and tropoelastin was performed and quantified.ResultsGene expression analysis highlighted a gradual upregulation of collagen mRNA genes. RNA-ISH confirmed upregulation of COL1A1 mRNA and showed a homogeneous distribution through the dermis. IHC showed increases in protein expression. Quantification revealed a 3.62-fold increase of procollagen type I (P < 0.0071), a 2.91-fold increase of TGF-β (P < 0.041), a 1.54-fold increase of HSP47 (P < 0.007), and a 1.57-fold increase of tropoelastin (P < 0.39) compared with untreated areas.ConclusionsThis study revealed significant induction of molecular and protein markers of type I collagen, which supports neocollagenesis and may play an essential role in clinically relevant skin improvement. A dermal remodeling process driven by increased TGF-β and higher expression of HSP47 was observed. Overall, these data provide the first evidence of dermal remodeling and clarify the mechanism by which cryolipolysis may induce skin improvement.Level of Evidence: 4

Ecological restoration guided by historical reference conditions can increase resilience to climate change of southwestern U.S. Ponderosa pine forests

Broad-scale forest restoration projects are implemented across the western United States to restore seasonally dry, frequent-fire-adapted ecosystems to improve ecological function and enhance resilience by increasing resistance to crown fire and climatic stressors. Despite the widespread use of restoration treatments that center on tree thinning and application of prescribed fire, the longevity of beneficial effects and the robustness of outcomes under future climate change predictions remains unclear. In this study, we remeasured a set of experimental areas established a minimum of 20 years ago that comprise a network of ponderosa pine (Pinus ponderosa) forest restoration study sites in northern Arizona. We analyzed ecological resiliency by evaluating forest conditions in terms of resistance to climatic stressors and potential crown fire in units that were thinned following evidence-based restoration guidelines (ERG), then burned with prescribed fire at multiple intervals, compared against paired untreated controls. Resilience indicators included forest structure, tree mortality, tree growth, regeneration, canopy fuels, and crowning index. We also simulated future forest conditions under a warming climate scenario (RCP 4.5) with a range of prescribed fire return intervals. Results indicated that experimental areas where restoration treatments were implemented remained more resilient to climate stressors compared to controls after 20 years. Treated areas had significantly lower tree mortality and greater average diameter growth compared to controls. Furthermore, forest structure generally remained similar to historical reference conditions in treated units with the exception of increases in ingrowth of sprouting species at the drier sites. Canopy fuel load and crown fire hazard in treated units remained significantly lower than controls, indicating that treatments remained effective in reducing crown fire potential over the 20-year study period without the need for additional tree thinning. Modeling basal area, crowning index, and the proportion of basal area in large trees under a future warming scenario suggested that the treated units underwent less changed than untreated areas. Under climate change, management of fire regimes even at longer-than-historical intervals (historical ≈ 5 yr, tested 5, 10, and 20 yr) would maintain basal area within our historical range of variability and maintain fire resistant forest over the next several decades. However, decline by the end of the century is concerning. Our results suggest that forest restoration treatments, guided by historical reference conditions, promote ecological resilience in the long-term and continued maintenance burning into the future is likely warranted even with continued drought and warming.

Comparison of phytoplankton control measures in reducing cyanobacteria assemblage of reservoirs found in the arid region of Southern Africa.

Ecological restorations of reservoirs are implemented worldwide; however, minimal successes are reported and understood for warmer African lakes like Swakoppoort Dam, Namibia. The objectives of the study were (a) to establish the effectiveness of the two control measures in reducing cyanobacteria growths in comparison with untreated control areas and (b) to compare the results generated before and after control measures with the reference Von Bach Dam. During Phoslock® treatment, the average cyanobacteria cells and total phosphate (TP) were 90,521cells/ml and 0.3mg/L in the treated area and 55,338cells/ml and 0.1mg/L in the control area. During Solar Powered Circulation (SPC) treatment, the average cyanobacteria cells were on average 906,420cells/ml in the treated areas and 121,891cells/ml in the control area. The TP on average was 0.3mg/L during SPC treatment, while during the combined treatment, the average cyanobacteria cells, TP, and total nitrogen (TN) were 18,387,226cells/ml, 0.27mg/L, and 2.41mg/L before and 22,836,511cells/ml, 0.42mg/L, and 1.50mg/L after treatment. This was higher compared to the reference site. PCA triplot indicates no grouping pattern, and the repeated-measures mixed model analyses indicate that treatment had no significant effect on cyanobacteria cells. It was evident that the two control measures were ineffective in reducing cyanobacterial cells. PRACTITIONER POINTS: Key findings of the article: Two phytoplankton control measures were found ineffective to reduce the cyanobacterial cell numbers. High cell numbers of cyanobacteria were recorded at the treatment areas compared to untreated control areas during both treatments. The combined effect of the two control measures was ineffective as more cyanobacterial cells were recorded during the treatment. During control measure treatment, the Swakoppoort Dam was hypertrophic, which could be due to a malfunctioned WWTP upstream. The inefficiency of the control measures could be due to small treatment area, higher nutrients, or treatment period. The implications of the results to water/wastewater practice: The selection of appropriate mitigation measures considering treatment area for dams with high nutrient situated in warmer arid environments. There is a need to understand the trophic relationships, climatic conditions, and the sources of the internal and external nutrients to manage water quality. Focus on point and non-point sources of nutrients as the root causes of the degradation of Swakoppoort Dam water.

Use of Multi-Temporal LiDAR to Quantify Fertilization Effects on Stand Volume and Biomass in Late-Rotation Coastal Douglas-Fir Forests

Forest fertilization is common in coastal British Columbia as a means to increase wood production and potentially enhance carbon sequestration. Generally, the effects of fertilization are determined by measuring sample plots pre- and post-treatment, resulting in fertilization effects being determined for a limited portion of the treatment area. Applications of remote sensing-based enhanced forest inventories have allowed for estimations to expand to the wider forested area. However, these applications have not focused on monitoring the effects of silvicultural treatments. The objective of this research was to examine if a multi-temporal application of the LiDAR area-based method can be used to detect the fertilization effects on volume, biomass, and height in a second-growth Douglas-fir (Pseudotsuga menziesii) stand. The study area on Vancouver Island was fertilized in January 2007, and sample plots were established in 2011. LiDAR acquisitions were made in 2004, prior to fertilization, and in 2008, 2011, and 2016, covering both treated and untreated areas. A total of 29 paired LiDAR blocks, comprised of four 20 m resolution raster cells, were selected on either side of the fertilization boundary for analysis of the effects across several different stand types differing in the percentage of Douglas-fir, site index, and age. Random forest (RF) plot-level models were developed to estimate total stem volume and total stem biomass for each year of LiDAR acquisition using an area-based approach. Plot level results showed an increase in stem volume by 13% fertilized over control from 2005 to 2011, which was similar to a 14% increase in above-ground carbon stocks estimated using a tree-ring stand reconstruction approach. Plot-level RF models showed R2 values of 0.86 (volume) and 0.92 (biomass) with relative cross-validated root mean square errors of 12.5% (volume) and 11.9% (biomass). For both the sample plots and LiDAR blocks, statistical results indicated no significant differences in volume or biomass between treatments. However, significant differences in height increments were detected between treatments in LiDAR blocks. The results from this research highlight the promising potential for the use of enhanced forest inventory methods to rapidly expand the assessment of treatment effects beyond sample plots to the stand, block, or landscape level.

608 Neuroimmune control of adult mammalian scarless skin regeneration

Adult mammalian wounds, with rare exception, heal with fibrotic scars that severely disrupt tissue architecture and function. Regenerative medicine seeks methods to avoid scar formation and restore the original tissue structures. We show in three adult mouse models that pharmacologic activation of the nociceptor TRPA1 on cutaneous sensory neurons reduces scar formation and can also promote tissue regeneration. Local activation of TRPA1 induces tissue regeneration on distant untreated areas of injury, demonstrating a systemic effect. Activated TRPA1 stimulates local production of interleukin-23 (IL-23) by dermal dendritic cells, leading to activation of circulating dermal IL-17–producing gd T cells. Genetic ablation of TRPA1, IL-23, dermal dendritic cells, or gd T cells prevents TRPA1-mediated tissue regeneration. These results reveal a cutaneous neuroimmune-regeneration cascade triggered by topical TRPA1 activators that promotes adult mammalian tissue regeneration, presenting a new avenue for research and development of therapies for wounds and scars.

Luminescence of Femtosecond Laser-Processed ZnSe Crystal

The ZnSe single crystal treatment in air environment with linearly polarized Ti/sapphire femtosecond (fs)laser pulses of the energy density of around 0.04-0.05 J/cm2 with central wavelength of 800 nm and the pulse duration of 140 fs at a repetition rate of 1 kHz generates the laser-induced periodic surface structures (LIPSSs). The setup with a cylindrical quartz lens at normal incidence allowed processing a relatively large area of the ZnSe sample in one pass of the laser beam. Morphology analysis of LIPSS by scanning electron microscopy (SEM) and image processing reveals the existence of two periods of around 200.0 nm and 630.0 nm simultaneously. All LIPSSs demonstrate the orientation perpendicular to the laser beam polarization. The possible nature of LIPSS formation on ZnSe single crystal is caused by the synergetic influence of the interference mechanism involving surface plasmon polaritons and hydrodynamic effects of surface morphology modification. The fs-laser-induced changes of carrier concentrations in ZnSe specify obtained periods of high spatial frequency LIPSS. The influence of femtosecond laser processing on luminescent properties of ZnSe single crystal has been studied by an analysis of the photoluminescence (PL) and X-ray luminescence (XRL) spectra of laser-treated and untreated areas in the visible region of spectrum at room and low temperatures. The PL spectra and XRL spectra, as well as temperature dependencies of XRL spectra or thermally stimulated luminescence curves, demonstrate a good correlation for untreated and fs-laser-treated ZnSe surfaces. Specific PL bands related to the extended structural defects do not appear for LIPSS at the ZnSe sample under an excitation of 337 nm (3.68 eV). The Relative intensities and position of separate components of observed luminescence bands after ultrashort laser treatment do not change significantly. Thus, the structural perfection of the ZnSe single crystal surface is preserved.

Persistent expression of interleukin-17 and downstream effector cytokines in recalcitrant psoriatic lesions after ustekinumab treatment.

The interleukin (IL)-23/T-helper (Th)17 axis is considered central to the pathogenesis of psoriasis, with IL-36γ considered a marker for histological differential diagnosis. However, expression data regarding key cytokines in the pathogenesis of psoriasis, as well as data on the effects of IL-23 inhibition on downstream cytokines in human psoriatic skin, are limited. We investigated the expression profile of key cytokines and the effect of ustekinumab (UST) on cytokine expression in human psoriatic tissue. Tumor necrosis factor (TNF)-α, IL-23, IL-17A, and IL-22 were highly expressed in the epidermis, dermal papillae, and upper dermis in patients with psoriasis compared with controls; IL-36γ was strongly expressed in the upper epidermis. Compared with the untreated group, expression intensity and area of IL-23 were significantly decreased in the UST group; expression areas of TNF-α, IL-17A, IL-22, and IL-36γ did not differ. This study identified the distribution and quantitative expression levels of key cytokines in psoriatic lesions and demonstrated that only IL-23 was downregulated without blocking downstream effector cytokines in recalcitrant psoriatic lesions during UST treatment. Our results suggest that, although IL-23 is inhibited, the persistent expression of IL-17 through an alternative pathway maintains the vicious cycle of the TNF-α/IL-23/IL-17 axis with IL-36γ, inducing refractory psoriatic lesions in patients with well-controlled psoriasis.

Multi-Electrode Architecture Modeling and Optimization for Homogeneous Electroporation of Large Volumes of Tissue

Electroporation is a phenomenon that consists of increasing the permeability of the cell membrane by means of high-intensity electric field application. Nowadays, its clinical application to cancer treatment is one of the most relevant branches within the many areas of electroporation. In this area, it is essential to apply homogeneous treatments to achieve complete removal of tumors and avoid relapse. It is necessary to apply an optimized transmembrane potential at each point of the tissue by means of a homogenous electric field application and appropriated electric field orientation. Nevertheless, biological tissues are composed of wide variety, heterogeneous and anisotropic structures and, consequently, predicting the applied electric field distribution is complex. Consequently, by applying the parallel-needle electrodes and single-output generators, homogeneous and predictable treatments are difficult to obtain, often requiring several repositioning/application processes that may leave untreated areas. This paper proposes the use of multi-electrode structure to apply a wide range of electric field vectors to enhance the homogeneity of the treatment. To achieve this aim, a new multi-electrode parallel-plate configuration is proposed to improve the treatment in combination with a multiple-output generator. One method for optimizing the electric field pattern application is studied, and simulation and experimental results are presented and discussed, proving the feasibility of the proposed approach.

Outdoor Thermal Comfort Study on a District Level as Part of the Housing Programs in Abu Dhabi and Al Ain, United Arab Emirates

The United Arab Emirates (UAE) has witnessed fast growth in urban development in the past four decades. A plan to build 7270 houses by 2021 has been initiated by the local authorities. Different local sustainability guidelines are being implemented, including the Public Realm Manual in Abu Dhabi. These local guidelines are tailored to consider the hot and arid climate of the UAE as well as the applied materials, the inclusion of greenery, shading devices, etc. Dubai, Abu Dhabi, and Al Ain are cities that have imposed the application of such guidelines. Additionally, the newly developed housing programs match the governmental plan. To understand the effect of these design programs on the outdoor thermal comfort (OTC), further investigations are necessary for each city. The most widely built prototype is detached villas, which result in untreated waste areas without shading or greenery. In the old local neighborhoods, Arabic houses were built next to each other to maximize the shading and to ease pedestrians’ walkability. This study aims to examine the districts where the housing programs are applied and to determine the most effective strategy to minimize the outdoor air temperatures and enhance walkability. The methodology implements the following processes in order: district analyses of the buildings as well as the externally applied materials, microclimate site measurements, ENVI-met (main software used) models of the current and future scenarios, results and recommendations. The strategies have different impacts in both cites due to the microclimate and other conditions.

An effective 'push-pull' control strategy for European tarnished plant bug, Lygus rugulipennis (Heteroptera: Miridae), in strawberry using synthetic semiochemicals.

European tarnished plant bug, Lygus rugulipennis (Heteroptera: Miridae), is a polyphagous pest damaging a range of arable and horticultural crops. Management is reliant upon chemical insecticides for control. These studies developed a synthetic semiochemical push-pull control strategy to reduce numbers of L. rugulipennis and subsequent fruit damage in UK strawberry crops. Using a series of small field experiments and testing in commercial strawberry crops we explored the efficacy of hexyl butyrate (HB) as the push element and female sex pheromone combined with phenylacetaldehyde as the pull element. HB dispensers placed 1.0, 3.5, 5.0 and 7.0 m from all-green Unitraps baited with L. rugulipennis female sex pheromone significantly reduced male catches by 99%, 54%, 44% and 20% compared with untreated control, respectively. Subsequently, in commercial crops, HB dispensers at 2-m intervals along the crop row (the push) combined with a perimeter pull reduced numbers of adult and nymph L. rugulipennis by up to 80% in organic strawberry crops compared with the untreated control. Finally, the push-pull system halved fruit damage (8%) compared with untreated areas (16%) in conventional crops. In organic strawberry crops, 90% of untreated strawberries had some mirid damage compared with only 41-51% in push-pull-treated areas. To our knowledge, this is the first demonstration of a push-pull approach using synthetic semiochemicals giving a significant reduction in crop damage by mirids and paves the way for non-pesticide control of a range of mirid species on multiple crops. © 2021 Society of Chemical Industry.

Graph-Analytical Method for Determining the Untreated and Treated with the Herbicide Area around the Stem with Over-lapping When the Working Body is Made in the Form of a Probe

In order to solve the problem of uniform surface treatment of bored areas of perennial plantations (treatment with herbicides) the graph-analytical method of examination of treatment quality (determination of untreated and treated area around the overlapped die) is proposed. To do this, you need to specify the diameter of the stamp of perennial planting, the overall size of the deflector in the form of a stylus, its position in the space relative to the diameter of the stamp of perennial planting, the size of the overlap zone when treating with herbicides in the gardens, the distance between the dies and the operating speed of the unit to determine the geometric and operating parameters of the sprayer. The whole stage of the working device (probe), when it is working in the nozzle zone is divided into 4 stages. Analysis of the graphical image of the stylus trajectory during movement around the stamp gives an idea of the surface treatment process with herbicides during spraying. In the first stage, part of the borehole zone is treated with overlap, while in the third stage, the untreated area around the die is left untreated. The dependencies of determining these areas depending on the geometric dimensions of the dies, their location in the gardens, the length and angle of the stylus and the operating speed of the unit are derived. According to the different trajectories of movement, in the process of influence of a stylus on a stamp there is a change of direction of an air-droplet stream, as a result of which the flow of a working liquid and accordingly the processed area changes. It is possible to determine the deviation of the actual rate of fluid flow from the specified rate at different points in the borehole area by calculating the areas treated with overlap and untreated. All obtained dependencies will serve for optimization of geometrical and mode parameters of the working device (feeler gauge) during its operation in the borehole zone.

Micro-catchment water harvesting-based rehabilitation ameliorated soil microbial abundance, diversity and function in a degraded dryland

Degraded drylands have a limited ecosystem functionality and require well-targeted rehabilitation interventions and sustainable land management for improvement. A promising rehabilitation package using mechanized micro-catchment water harvesting, to support the development of out-planted native shrub seedlings, is being introduced in Jordan's Badia. However, the impacts of rehabilitation on the soil microbial communities through the changes in soil physicochemical properties, and microbial potential contribution to soil function and stability are unclear. In the present study, soil microbial properties (abundance, community structure, community composition, diversity, network complexity, and decomposition function) and their relations with selected physicochemical properties (moisture, pH, salinity, and organic matter quantity and quality) were investigated in the micro-catchment water harvesting bunds in comparison with those in untreated interspace areas four years after implementation. At the bunds, fungal and bacterial abundances increased significantly, as did prokaryotic diversity, prokaryotic function to produce decomposing enzymes, and the redundancy of these functions. Furthermore, Burkholderiales (plant growth-promoting bacteria) and Cytophagales (cellulolytic bacteria) increased in the bunds. Enhanced soil moisture, through the rehabilitation, was likely a key for the improvements of the microbiota, including the increases in the abundances of fungi and the specific bacteria (Burkholderiales and Cytophagales), most of the decomposition function, and the functional redundancy. Furthermore, the decrease in salinity due to leaching resultant from the infiltration of collected surface runoff at the bunds likely supported an increase in diversity and parts of the function. The results suggest that the rehabilitation has multiple beneficial impacts on soil microbial communities, which further contribute to long-term ecosystem functionality and stability.

House Prices, Government Quality, and Voting Behavior

The hypothesis that minority voters act in their economic self-interest in supporting municipal candidates of their own race or ethnicity has been tested using changes in municipal spending and employment. However, governments affect voter welfare in many other ways, particularly in the provision of quality public services. This paper exploits a natural experiment arising in 1995 when Congress created an appointed financial control board for the District of Columbia and gave to it many of the powers previously reserved to the local elected government. The natural experiment sets up a triple-difference research design based on changes in property values across District neighborhoods and voting behavior compared to untreated adjacent areas across the District boundary in Maryland. We find that oversight by the control board raised annual appreciation rates in the District by 178 to 254 basis points during the period from 1995 to 2001. Furthermore, effects of the intervention were uniformly positive across areas of the District that varied dramatically in their support for the incumbent administration that lost its powers. This implies, after the fact, that the administration’s supporters had not voted in accord with economic self-interest.

The use of disorientation of the plum moth in the conditions of chemical protection of the orchard

Disorientation of the plum moth (Grapholita funebrana Treitschke) under the conditions of chemical protection of the garden was carried out using dispensers (Z8DDA, 16.7 mg) produced by ZAO Shchelkovo Agrokhim. Dispensers were placed at a tree trunk at a height of 1.5 - 2 m with an application rate of 500 per hectare. The study was carried out on three plots of a 1 ha plum orchard: a plot with chemical treatments and with disorientation, an area with chemical treatments, without disorientation, and an area without chemical treatments and without disorientation. The effectiveness of disorientation at a high abundance during the flight peaks of the second and third generations of the pest ranged from 100 % to 92-93 %. The effectiveness of disorientation, together with the chemical protection of the garden in comparison with untreated area, showed higher results - in the first two months, the efficiency ranged from 100 to 98 %, and during the periods of the 2nd and 3rd flight peaks of the pest (with a flight intensity of 136 - 120 individuals per trap) did not fall below 96 %.

An entomological survey in the Sudanese Guinean environmental transition zone after indoor residual spraying, Chad.

Introductionmalaria is a major public health issue in Africa. In Chad in 2019, with 955,243 confirmed cases and 2,955 deaths, malaria is the main cause of consultations. A longitudinal entomological study was conducted in Moïssala Health District. Its objective was to assess the impact of indoor residual spraying with 80% bendiocarb wettable powder on malaria transmission.Methodstwo areas were defined for the study: Dembo, located in the sprayed area, Moïssala, in the untreated area. Two sampling methods were used: pyrethrum spray catches and human landing catches.Resultssixteen sessions of human landing catches totalling 32 man-nights were conducted and 160 rooms/site were sprayed. Two anopheles were captured in Dembo and 547 in Moïssala. In Moïssala, An coluzzii, An funestus and An rufipes were captured in the rooms and on human bait. An colluzzii and An funestus were captured in pyrethrum spray catches in Dembo. The anophelian human landing catches density was zero in Dembo while it was 8.38 bites/man/night in outdoor and 10.06 bits/man/night in indoor in Moïssala. Only An coluzziiwas found infected in human landing catches and sporozoite index of was 7.46% (10/134) in outdoor and 7.45% (12/161) in indoor in Moïssala. Malaria transmission was estimated at 0.63 infected bites/man/night in outdoor and 0.75 infected bites/man/night in indoor i.e. 229.95 infected bites/man/year in outdoor and 273.75 infected bites/man/year. In pyrethrum spray catches, An coluzzii and An rufipes were the two species found infected in Moïssala with sporozoite indices of 6.70% (23/343) and 20% (2/10) respectively. However, in Dembo, neither of the two captured mosquitoes was found infected.Conclusionthe indoor residual spraying campaign in the eastern zone of Moïssala has led to the collapse of vectors´ density and aggressiveness. However, its evaluation over a short period of time is not sufficient to assess the impact of malaria transmission in this constant and endemic malaria zone.

Using Satellite Imagery and Machine Learning to Estimate the Livelihood Impact of Electricity Access

In many regions of the world, sparse data on key economic outcomes inhibits the development, targeting, and evaluation of public policy. We demonstrate how advancements in satellite imagery and machine learning can help ameliorate these data and inference challenges. In the context of an expansion of the electrical grid across Uganda, we show how a combination of satellite imagery and computer vision can be used to develop local-level livelihood measurements appropriate for inferring the causal impact of electricity access on livelihoods. We then show how ML-based inference techniques deliver more reliable estimates of the causal impact of electrification than traditional alternatives when applied to these data. We estimate that grid access improves village-level asset wealth in rural Uganda by 0.17 standard deviations, more than doubling the growth rate over our study period relative to untreated areas. Our results provide country-scale evidence on the impact of a key infrastructure investment, and provide a low-cost, generalizable approach to future policy evaluation in data sparse environments.