Unloaded Condition Research Articles

Impact of Implant Surface and Smoking on Peri-Implant Human Bone: What we Learned from The Last 20 Years?

The present review summarizes the findings from human histological studies conducted over the past 20 years at the University of Guarulhos, Brazil, examining the impact of various implant surface topographies and smoking on peri-implant bone response. Seven different implant surfaces were evaluated in 90 partially or completely edentulous individuals using a total of 123 micro-implants. Histometric parameters, including bone-implant contact (BIC%), bone area within the threads (BA%), and bone density (BD), were assessed after an 8-week healing period. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses were also performed. Results showed that treated surfaces, regardless of the treatment type, consistently demonstrated better histometric outcomes compared to machined surfaces. Anodized surfaces and those subjected to airborne particle abrasion, followed by acid etching, exhibited higher BIC% values than machined surfaces in smoker patients. Smoking reduced BIC% around anodized implants. The presence of inflammatory cells was observed adjacent to the peri-implant soft tissue on some treated surfaces. In conclusion, implant surface topography significantly influences early bone response under unloaded conditions, with treated surfaces promoting better human bone tissue response than machined surfaces. However, smoking negatively impacts peri-implant bone healing, emphasizing the importance of smoking cessation for optimal osseointegration.

The Acute Effects of Velocity Loss During Half Squat Exercise on Jump Performance

The velocity loss (VL) approach during squat exercise may increase the post-activation potentiation enhancement effect on squat jump performance. If this method succeeds, then different conditions of VL should be researched before its implementation to the field. This study hypothesized that squat jump performance would be increased after different volumed VL conditions during half-squat exercise. Eighteen resistance-trained men (mean [M] ± standard deviation [SD]; age: 24.00±3.53 years; body mass: 78.37±5.53 kg; height: 179.35±7.04 cm; one-repetition maximum (1RM) half squat: 110.85±11.92 kg) voluntarily performed squat jump under unloaded and four different VL conditions (R6: six repetitions, Ruf: repetitions until failure, VL10: velocity loss thresholds 10%, VL20: velocity loss thresholds 20%) after a set of half-squat exercises at 80% of one-repetition maximum separated by at least 72 hours. The results revealed that subjects demonstrated significantly better squat jump performance in VL10, VL20, and R6 conditions than the unloaded and Ruf conditions (p

Design and validation of a novel knee biomechanical test method

A novel structural dynamics test method and device were designed to test the biomechanical effects of dynamic axial loading on knee cartilage and meniscus. Firstly, the maximum acceleration signal-to-noise ratio of the experimental device was calculated by applying axial dynamic load to the experimental device under unloaded condition with different force hammers. Then the experimental samples were divided into non-specimen group (no specimen loaded), sham specimen group (loaded with polypropylene samples) and bovine knee joint specimen group (loaded with bovine knee joint samples) for testing. The test results show that the experimental device and method can provide stable axial dynamic load, and the experimental results have good repeatability. The final results confirm that the dynamic characteristics of experimental samples can be distinguished effectively by this device. The experimental method proposed in this study provides a new way to further study the biomechanical mechanism of knee joint structural response under axial dynamic load.

Lower limbs micro-loading acutely attenuates repeated change-of-direction performance in male youth during small-sided soccer games

BackgroundSoccer players often wear light-weighted wearable resistance (WR) attached to different body parts during the warm-up period with the aim to improve measures of physical fitness. However, the effect of WR on physical performance is unknown. This study evaluated the effects of WR with different micro-loadings on repeated change-of-direction (RCoD) performance while executing small-sided soccer games (SSG).MethodsTwenty male soccer players aged 16.0 ± 1.5 years (body mass 74.0 ± 7.4 kg, body-height 175.0 ± 10.0 cm) volunteered to participate in this study. Following a within-subject study design, players performed four specific warm-up protocols in randomized order with a rest of 72 h between protocols: (1) WR micro-loadings with 0.1% of body mass (WR0.1); (2) WR micro-loadings with 0.2% of body mass (WR0.2); (3) WR micro-loadings with 0.3% of body mass (WR0.3); (4) no WR (control = CONT). After the warm-up protocols, players performed 2 sets of 20-min SSG. The RCoD was collected at the 8th min of SSG (SSG 1–8 min), the 15th min of SSG1 (SSG1-15 min), and at the 15th min of SSG2 (SSG2-15 min). Outcomes included mean and total RCoD indices (i.e., mean time and total time for each condition).ResultsBased on the outcomes of a two-way repeated measures analysis of variance (ANOVA), WR0.1 and WR0.2 were more effective than control in dampening the decrease of RCoD’s total time during SSG1-8 min, and SSG2-15 min (small ES: 0.24–0.35; p < 0.05). However, no significant differences were observed between WR0.3 and control. In addition, WR0.1 and WR0.2 significantly affected the decreases in RCoD’s mean best time during SSG1 and SSG2 which was observed in the unloaded condition (CONT) and consequently displayed a lower rate of RCoD performance decrease.ConclusionThis study reports that wearing lower extremity WRs with micro-loads of 0.1% or 0.2% of body mass attenuates physical fatigue indicated in attenuated RCoD performance while executing SSG.

Quantitative analysis of microstructure evolution, stress partitioning and thermodynamics in the dynamic transformation of Fe-14Ni alloy

Dynamic transformation (DT) of austenite (γ) to ferrite (α) in the hot deformation of various carbon steels was widely investigated. However, the nature of DT remains unclear due to the lack of quantitative analysis of stress partitioning between two phases and the uncertainty of local distribution of substitutional elements at the interface in multi-component carbon steels used in the previous studies. Therefore, in the present study, a binary Fe-Ni alloy with α + γ duplex microstructure in equilibrium was prepared and isothermally compressed in α + γ two-phase region to achieve a quantitative analysis of microstructure evolution, stress partitioning, and thermodynamics during DT. γ to α DT during isothermal compression and α to γ reverse transformation on isothermal annealing under unloaded condition after deformation were accompanied by Ni partitioning. The lattice strains during thermomechanical processing were obtained via in-situ neutron diffraction measurement, based on which the stress partitioning behavior between γ and α was discussed by using the generalized Hooke's law. A thermodynamic framework for the isothermal deformation in solids was established based on the basic laws of thermodynamics, and it was shown that the total Helmholtz free energy change in the deformable material during the isothermal process should be smaller than the work done to the deformable material. Under the present thermodynamic framework, the microstructure evolution in the isothermal compression of Fe-14Ni alloy was well explained by considering the changes in chemical free energy, plastic and elastic energies, and the work done to the material. In addition, the stabilization of the soft α phase in Fe-14Ni alloy by deformation was rationalized since the γ to α transformation decreased the total Helmholtz free energy by decreasing the elastic and dislocation energies.

QUANTITATIVE ULTRASOUND AND MRI IMAGING OF THE BUTTOCKS AND THIGHS IN A SEATED POSITION

Pressure ulcers are a common occurrence in individuals with spinal cord injuries, and are attributed to prolonged sitting and limited mobility. This therefore creates the need to better understand soft tissue composition, in the attempt to prevent and treat pressure ulcers. In this study, novel approaches to imaging the soft tissue of the buttocks were investigated in the loaded and unloaded position using ultrasound (US) and magnetic resonance imaging (MRI).Twenty-six able-bodied participants (n=26, 13 males and 13 females) were recruited for this study and 1 male with a spinal cord injury. Two visits using US were required, as well as one MRI visit to evaluate soft tissue thickness and composition. US Imaging for the loaded conditions was performed using an innovative chair which allowed image acquisition in the seated upright position and MRI was done in the lateral decubitus position and loading was applied to the buttocks using a newly developed MRI compatible loader. The unloaded condition was a lateral decubitus position. Soft tissue was measured between the peak of the ischial tuberosity (IT) and the proximal femur and skin.Tissue thickness reliability for US was excellent, ICC=0.934–0.981 with no significant differences between the scan days. US and MRI measures of tissue thickness were significantly correlated (r=0.68–0.91). US underestimated unloaded tissue thicknesses with a mean bias of 0.39 – 0.56 for total tissue and muscle + tendon thickness. When the buttocks were loaded, total tissue thickness was reduced by 64.2±9.1%.US assessment of soft tissue thicknesses was reliable in both positions. The unloaded measurements using US were validated with MRI with acceptable limits of agreement, albeit tended to underestimate tissue thickness. Tissue thickness, but not fatty infiltration of muscle played a role in how the soft tissue of the buttocks responded to loading.

Therapeutic potential of human induced pluripotent stem cell–derived cardiac tissue in an ischemic model with unloaded condition mimicking left ventricular assist device

ObjectiveThis study aimed to explore the therapeutic potential of human induced pluripotent stem cell (hiPSC)-derived cardiac tissues (HiCTs) in the emerging approach of bridge to recovery for severe heart failure with ventricular assist devices. We used a rat model of heterotopic heart transplantation (HTx) to mimic ventricular assist device support and heart unloading. MethodsHiCTs were created by inserting gelatin hydrogel microspheres between cell sheets made from hiPSC-derived cardiovascular cells. Male athymic nude rats underwent myocardial infarction (MI) and were divided into the following groups: MI (loaded, untreated control), MI + HTx (unloaded, untreated control), MI + HTx + HiCT (unloaded, treated), and MI + HiCT (loaded, treated). HiCTs were placed on the epicardium of the heart in treated groups. We evaluated HiCT engraftment, fibrosis, and neovascularization using histologic analysis. ResultsAfter 4 weeks, HiCTs successfully engrafted in 5 of 6 rats in the MI + HTx + HiCT group (83.3%). The engrafted HiCT area was greater under unloaded conditions (MI + HTx + HiCT) than loaded conditions (MI + HiCT) (P < .05). MI + HTx + HiCT had a significantly smaller infarct area compared with MI and MI + HTx. The MI + HTx + MiCT group exhibited greater vascular density in the border zone than MI and MI + HTx. HiCT treatment suppressed cardiomyocyte atrophy due to left ventricular unloading (P = .001). The protein level of muscle-specific RING finger 1, an atrophy-related ubiquitin ligase, was lower in the MI + HTx + HiCT group than in MI + HTx (P = .036). ConclusionsTransplanting HiCTs into ischemic hearts under unloaded conditions promoted engraftment, neovascularization, attenuated infarct remodeling, and suppressed myocyte atrophy. These results suggest that HiCT treatment could contribute to future advancements in bridge to recovery.

Extrusion of the medial meniscus under a weight-loading condition in early knee osteoarthritis: an investigation using special upright magnetic resonance imaging

BackgroundWhether the medial meniscus morphology and movement occur under upright loading conditions in early knee osteoarthritis (OA) or medial meniscus posterior root tear (MMPRT) remains unknown. This study aimed to evaluate the medial and anteroposterior extrusion of the medial meniscus under unloaded and upright-loaded conditions in patients with early knee OA.MethodsTwelve patients with early knee OA and 18 healthy adult volunteers participated in this study. Magnetic resonance imaging using special equipment was performed with the participants in the unloaded and upright-loaded conditions. Medial, anterior, and posterior extrusions of the medial meniscus against the tibial edge were evaluated and compared between the early knee OA and healthy adult control groups. Additionally, 12 patients in the early knee OA group were divided into 2 subgroups based on whether MMPRT was observed, and the extrusion of the medial meniscus was compared.ResultsThe amount of medial extrusion of the medial meniscus in both the unloaded and upright-loaded conditions was significantly greater in the early knee OA group than in the control group (unloaded: 2.6 ± 1.0 mm vs 0.7 ± 0.5 mm; upright-loaded: 3.7 ± 0.9 mm vs 1.8 ± 0.8 mm). Similarly, the anterior and posterior extrusion of the medial meniscus in the upright-loaded condition was significantly larger in the early knee OA group (anterior: 4.6 ± 1.0 mm vs 3.7 ± 1.1 mm; posterior: -3.4 ± 1.1 mm vs -4.6 ± 1.6 mm). However, no difference was observed in meniscal extrusion between unloaded and upright-loaded conditions. The posterior extrusion of the medial meniscus in the upright-loaded condition was significantly greater in MMPRT cases than in non-MMPRT cases in the early knee OA group (MMPRT: -2.7 ± 1.1 mm; non-MMPRT -4.1 ± 1.5 mm).ConclusionsIn early knee OA, significantly large meniscal extrusions of the medial meniscus in both unloaded and upright-loaded conditions were found compared with healthy adults. Among patients with early knee OA, those with MMPRT showed a large posterior extrusion of the medial meniscus in the upright-loaded condition compared with those without MMPRT.Level of evidenceLevel IV.

Overall Design of Multi-module Offshore Floating Bridge and a Method for Random Analysis of Anchor Tension

This paper proposes a multi-module floating bridge design for offshore applications and establishes a coupled motion response analysis method for the anchoring system of the floating bridge. Time-domain coupled analysis is performed under the combined effects of wind, wave, and current loads, resulting in the maximum tension of the anchor chain in random waves. Using the typical island and reef sea area of Zhoushan, China as an example, the randomness of waves is considered, and a random sampling method is adopted. The main configuration, connection structure, and mooring system design schemes are presented, and the maximum tension of the anchoring system is numerically calculated and analyzed. The random distribution of the maximum anchor chain tension is obtained, the goodness-of-fit test is conducted for the fitting results, and the main degree-of-freedom motion response is analyzed under different exceedance probabilities. The results show that the multi-module offshore floating bridge meets the design requirements, and the statistical characteristics of the maximum tension of the anchor chain under random waves conform to the Gumbel distribution. The mode of the random distribution of the maximum anchor chain tension increases by 7.65% when fully loaded compared to the unloaded condition. In different exceedance probability scenarios, there are significant differences in the maximum tension of the anchor chain and the motion response of the main degrees of freedom of the floating bridge.

CHANGE OF DIRECTION SPEED UNDER TWO LOADING CONDITIONS AMONG FEMALE POLICE OFFICERS: ASSOCIATION WITH BODY MORPHOLOGY

Change of direction speed (CODS) is an important performance ability for police officers. This is even more emphasized when officers perform tasks while carrying their occupational load (e.g., protective vest, weapon, radio, cuffs, etc.). The absolute weight of the equipment remains the same regardless of officer’s body size and weight, which is of importance for female police officers whose morphology is different than in males. This study investigated the associations between selected measures of body morphology and CODS among female police officers under two loading conditions. The sample consisted of 29 female police officers (age = 32.00±5.09 yrs, body height = 162.92±5.01 cm, and body mass = 70.88±13.42 kg). Anthropometric variables included height, weight, and body mass index (BMI), while body composition characteristics included percent body fat, (PBF), percentage of skeletal muscle mass (PSMM), and index of hypokinesia (IH). CODS was assessed using the Illinois agility tests under loaded (LIAT) (10 kg vest) and unloaded (IAT) conditions. Participants’ CODS times were significantly slower in the LIAT condition (p &lt; 0.001). IAT correlated to BMI (r=0.479, p&lt;0.05), PBF (r=0.647, p&lt;0.001), PSMM (r=-0.655, p≤0.001), and IH (r=0.462, p&lt;0.05). Similarly, LIAT was associated with BMI (r=0.446, p&lt;0.05), PBF (r=0.651, p&lt;0.001), PSMM (r=-0.672, p&lt;0.001), and IH (r= 0.503, p&lt;0.01). These findings highlight the need for developing specific physical training programs aimed at improving and maintaining healthy body composition levels among female officers if improved CODS is the goal.

Comparison of effects of peripheral vasculature on tonometric radial pulse and cuff-based brachial pulse waveform as used in estimation of central aortic pressures.

Aortic pressure estimation requires reliable peripheral pulse waveform acquisition. The peripheral waveform can change with local vascular effects that can be independent of aortic pressure. This study quantifies the effects of peripheral vasculature changes on radial and brachial waveforms. In 20 subjects (37± 15 years, 7 female), brachial volumetric displacement (cuff-based) and radial tonometry waveforms were simultaneously measured whilst a cuff around the hand on the same arm was inflated to induce transmural pressures of -60, -30, -15, 0, 15 and 30 mmHg, altering local peripheral resistance and compliance by graded arterial wall unloading. Aortic blood pressure (BP), augmentation index (AIx) and ejection duration were calculated from the measurements using a generalized transfer function. The parameters under unloaded conditions were compared to baseline measurements. Brachial systolic and diastolic BP did not change throughout the experiment. Altering peripheral resistance and compliance did not significantly change calculated aortic BP values, although changes were nominally greater for radial (maximum +8±1 mmHg) compared to brachial (maximum +2±1 mmHg) waveforms. AIx at 0 mmHg transmural pressure (maximum arterial wall unloading) was higher when derived from radial waveforms (+24±3%, p<0.001) but not when derived from brachial waveforms. Localized changes in peripheral resistance and compliance affect tonometer acquired radial waveforms but not volumetric displacement acquired brachial pressure waveforms, as judged by computed central aortic augmentation pressure parameters. This suggests aortic pressure estimation from the brachial cuff waveform is less sensitive to peripheral vasculature disturbances that alter the peripheral arterial pulse morphology.

First Tarsometatarsal Joint Fusion in Foot-A Biomechanical Human Anatomical Specimen Analysis with Use of Low-Profile Nitinol Staples Acting as Continuous Compression Implants.

Background and Objectives: The aim of this study was to investigate under dynamic loading the potential biomechanical benefit of simulated first tarsometatarsal (TMT-1) fusion with low-profile superelastic nitinol staples used as continuous compression implants (CCIs) in two different configurations in comparison to crossed screws and locked plating in a human anatomical model. Materials and Methods: Thirty-two paired human anatomical lower legs were randomized to four groups for TMT-1 treatment via: (1) crossed-screws fixation with two 4.0 mm fully threaded lag screws; (2) plate-and-screw fixation with a 4.0 mm standard fully threaded cortex screw, inserted axially in lag fashion, and a 6-hole TMT-1 Variable-Angle (VA) Fusion Plate 2.4/2.7; (3) CCI fixation with two two-leg staples placed orthogonally to each other; (4) CCI fixation with one two-leg staple and one four-leg staple placed orthogonally to each other. Each specimen was biomechanically tested simulating forefoot weightbearing on the toes and metatarsals. The testing was performed at 35-37 °C under progressively increasing cyclic axial loading until construct failure, accompanied by motion tracking capturing movements in the joints. Results: Combined adduction and dorsiflexion movement of the TMT-1 joint in unloaded foot condition was associated with no significant differences among all pairs of groups (p ≥ 0.128). In contrast, the amplitude of this movement between unloaded and loaded foot conditions within each cycle was significantly bigger for the two CCI fixation techniques compared to both crossed-screws and plate-and-screw techniques (p ≤ 0.041). No significant differences were detected between the two CCI fixation techniques, as well as between the crossed-screws and plate-and-screw techniques (p ≥ 0.493) for this parameter of interest. Furthermore, displacements at the dorsal and plantar aspects of the TMT-1 joint in unloaded foot condition, together with their amplitudes, did not differ significantly among all pairs of groups (p ≥ 0.224). Conclusions: The low-profile superelastic nitinol staples demonstrate comparable biomechanical performance to established crossed-screws and plate-and-screw techniques applied for fusion of the first tarsometatarsal joint.

Long-term flexural response of reinforced calcium sulfoaluminate/cement concrete beams

Calcium sulfoaluminate binder is an up-to-date solution for reducing the environmental impact of construction industry. Several studies showed satisfactory mechanical performance of concrete realized with such binder, as well as a significant CO2 emission reduction compared to ordinary Portland cement. However, a lack of knowledge on the mechanical behaviour of calcium sulfoaluminate concrete elements still exists. Particularly, a limited number of studies on long-term mechanical response is available in the literature. In this work, an investigation on full-scaled reinforced concrete beams made of calcium sulfoaluminate binder is reported, aiming at analysing their long-term response in loaded and un-loaded conditions. Two sets of beams, with different calcium sulfoaluminate and ordinary Portland cement percentage were realized. In the first set, serviceability performance level load was applied, while no loading condition was considered in the second set. Both sets were subjected to long-term exposure in ordinary environment and subsequently tested through four point-bending loading up to failure. The non-linear load–deflection response was deeply analysed alongside that of virtually identical unexposed beams tested in a previous experimental campaign. The experimental results were compared to analytical predictions to assess the reliability of existing formulations in reproducing short- and long-term behaviour. A satisfactory long-term performance of the beams realized using calcium sulfoaluminate binder was observed, confirming the suitability of this technology for structural applications. On the other hand, results evidenced the need of updating existing analytical formulations to accurately predict deformation capacity and crack pattern in calcium sulfoaluminate binder elements.

Multi-modality imaging evaluation and pre-surgical planning for aortic valve-sparing operations in patients with aortic root aneurysm.

Cardiac computed tomography (CT) and magnetic resonance (CMR) supplement echocardiography in the evaluation of those with aortic root and ascending aortic dilation, determining timing for intervention, guiding pre-surgical planning and post-operative surveillance. The dynamic, three-dimensional complexity of the aortic root and how it relates to the base of the left ventricle must be understood in any surgical approach addressing the aneurysmal aortic root. With improved imaging technology and the importance for proper patient counseling, it is no longer acceptable to enter the operating theater without a detailed blue print of what the problem is, and how best to address it. In addition, reliance on surgical expertise alone for intraoperative evaluation and decision making could be suboptimal due to the unloaded condition of the aortic root and the variance of experience of the surgeons to successfully repair the aortic valve. This is exemplified by the selective surgeons and centers who have the ability to tackle these aortic valve and root pathologies, compared to mitral valve repair techniques that have been codified and are generalizable. This review discusses a multimodality imaging approach in the patient with aortic root aneurysm, focusing on the precision added with pre-surgical CT assessment to guide aortic-valve sparing operations. This precision is afforded with a detailed understanding of the anatomy of the aortic root and underlying support, and its accurate evaluation by standard two- and three-dimensional imaging. Furthermore, we describe the evolving ability to predict the location of ventricular components of the atrioventricular conduction axis with further clinical imaging to personalize surgical strategies.

Effect of static and dynamic loading of backpack on cervical and shoulder posture in school going children of age group 10-15 years old - pre-test and post -test experimental study

Abstract: Background: Musculoskeletal problems associated with use of backpack have become an increasing concern with school children due to its direct and indirect effect on health. School bag loads are reported to cause many problems in children such as body pain, cardio?respiratory changes, postural changes, and balance impairment. Method: A pre-test and post-test experimental study carried out to study the effect of static and dynamic loading of backpack on cervical and shoulder posture in school going children of age group 10-15 years old. Result: The result shows that there is significant effect of static and dynamic loading of backpack on cervical and shoulder posture in school going children of age group 10–15 years old.Conclusion: The study concludes that backpack weighing 10% of body weight shows significant changes in cervical and shoulder posture. These changes are more after dynamic activities when compared with static loading and unloaded condition.

Determination of Loading and Residual Stresses on Offshore Jacket Structures by X-ray Diffraction

As basements of offshore wind turbines (OWTs) in deep water (&gt;50 m), jacket structures are an economic alternative to monopiles. For this reason, the structural durability of jackets has become more important. In such structures, welded tubular joints are weak points for fatigue design. The harmful effect of tensile residual stresses in welding joints is well known. For these reasons, the residual stresses and the loading stresses of offshore jacket structures were determined by X-ray diffraction (XRD) using a mobile diffractometer. This allows us to directly determine the load stress at the fatigue-critical locations, namely at the weld toe at the testing rig. High tensile residual stresses up to 250 MPa were determined in a welded (and unloaded) condition. At a loaded structure (10,000 load cycles), a lower residual stress level was determined. During loading, a local increase in the stress at the welded joint that is between 1.4 and 4 times higher than the applied nominal stress was determined. Furthermore, it is shown that additional treatment (grinding and clean blasting) influences the local stress state significantly.

Reinforcement of Hydrogels with a 3D-Printed Polycaprolactone (PCL) Structure Enhances Cell Numbers and Cartilage ECM Production under Compression

Hydrogels show promise in cartilage tissue engineering (CTE) by supporting chondrocytes and maintaining their phenotype and extracellular matrix (ECM) production. Under prolonged mechanical forces, however, hydrogels can be structurally unstable, leading to cell and ECM loss. Furthermore, long periods of mechanical loading might alter the production of cartilage ECM molecules, including glycosaminoglycans (GAGs) and collagen type 2 (Col2), specifically with the negative effect of stimulating fibrocartilage, typified by collagen type 1 (Col1) secretion. Reinforcing hydrogels with 3D-printed Polycaprolactone (PCL) structures offer a solution to enhance the structural integrity and mechanical response of impregnated chondrocytes. This study aimed to assess the impact of compression duration and PCL reinforcement on the performance of chondrocytes impregnated with hydrogel. Results showed that shorter loading periods did not significantly affect cell numbers and ECM production in 3D-bioprinted hydrogels, but longer periods tended to reduce cell numbers and ECM compared to unloaded conditions. PCL reinforcement enhanced cell numbers under mechanical compression compared to unreinforced hydrogels. However, the reinforced constructs seemed to produce more fibrocartilage-like, Col1-positive ECM. These findings suggest that reinforced hydrogel constructs hold potential for in vivo cartilage regeneration and defect treatment by retaining higher cell numbers and ECM content. To further enhance hyaline cartilage ECM formation, future studies should focus on adjusting the mechanical properties of reinforced constructs and exploring mechanotransduction pathways.

Permittivity measurement of biological tissues using interdigital capacitor based resonator

A CPW resonator etched on the central conductor is presented to measure the complex permittivity of biological tissues. In the unloaded condition, the proposed structure works as a resonator with a resonant frequency of 5.625 GHz. The proposed sensor is able to cover a wide range of permittivity measurement from approximately 5 (fat tissue) to 70 (malignant tissue) with sensitivity of 4.01 % and FDR( Frequency Detection Resolution) of 270 MHz . Breast phantoms with high fidelity need to be fabricated to imitate the various layers of the breast like skin, fat, glandular tissue, and tumor tissue regarding permittivity with different chemicals in different proportions. The major challenge in fabricating such phantoms is in creating an appropriate combination of substances to get the required characteristics for these biological tissues. A sample of various tissue types was fabricated using Gelatin and safflower-based recipes. The complex permittivity tissue phantoms are measured using a fabricated resonator. The measured dielectric characteristics of biological tissues are in good agreement with the theoretical values from the database.

Right ventricular function in hearts of a mouse model of Duchenne muscular dystrophy following increased ventricular load

Duchenne muscular dystrophy (DMD) is an X-linked recessive myopathy caused by mutations in the gene encoding dystrophin. Dystrophin is present in striated muscle cells and stabilizes the sarcolemma to mitigate contraction-induced damage. The main cause of death in DMD is cardiopulmonary failure by age 30. Diaphragmatic weakness in DMD causes restrictive lung disease and excessive afterload on the right ventricle (RV). Thus, RV dysfunction in DMD patients is typically observed early in disease progression. The objective of this study was to investigate ex vivo RV function in a mouse model of muscular dystrophy and test the hypothesis that RV function is impaired following a sustained increase in ventricular preload and afterload. Right-ventricular pressure development (P dev ) and rate of pressure decay (dP/dt Min ) were monitored in isolated perfused hearts of 2-6 month normal (C57BL/6, n=5) and dystrophic (mdx 4CV , n=5) male mice. Measurements were obtained under unloaded conditions and during right-ventricular load challenge (10 mmHg preload; 20 mmHg afterload). Coronary effluent lactate dehydrogenase (LDH) levels were measured following 30 minutes of sustained load challenge to assess cardiac damage. Our results show dystrophic hearts exhibited greater RV P dev than normal hearts under unloaded conditions (24±2 mmHg mdx 4CV vs 15±2 mmHg C57BL/6, P=0.015). In response to load challenge, P dev increased (P&lt;0.05) in both groups, but it was similar between dystrophic and normal hearts (32±1 mmHg mdx 4CV vs 33±3 mmHg C57BL/6). Rate of pressure decay was similar between dystrophic and normal hearts under both conditions. Levels of LDH following load challenge were higher (P&lt;0.05) in dystrophic versus normal hearts. In conclusion, in the absence of ventricular load RV contractile function was enhanced in dystrophic hearts. With RV load both dystrophic and normal hearts exhibited similar contractile function, yet sustained load challenge increased cellular damage in dystrophic versus normal hearts. National Institutes of Health, University of Missouri School of Medicine, University of Missouri System This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Mechanical behavior of additively and conventionally manufactured 316L stainless steel plates joined by gas metal arc welding

Combining several additive manufactured (AM) parts to larger parts by welding may be required due the limited building volume of powder bed AM methods. Laser powder bed fusion (LPBF) has a great potential because it enables the production of nearly full-density components through AM processes; however, additional residual stresses and production defects are induced by LPBF. These residual stresses affect the residual stress state of welded AM parts. In combination with the production related defects, both alter the mechanical and—in particular—the fatigue behavior of these welded joints. In this study, various tests are performed to characterize the butt joints of 316L AM steel plates made by gas metal arc welding. To this goal, joints are produced with weld seams parallel and vertical to the layer orientation of AM plates. The results are compared to joints of conventionally rolled steel plates produced with the same welding parameter. The residual stress states in initial (unloaded) condition and after cyclic loading were determined by X-ray diffraction techniques for AM and rolled plates. Complex residual stress states were determined at the welds made of AM steel plates compared to the welds made of rolled steel plates; however, the residual stress level in the heat affected zone of the butt-welded AM steel plates was similar to the welds made of hot-rolled steel plates. After cyclic loading with a high load level, high residual stress relaxations were observed in the parent materials. The fatigue design curve for butt joints from international standards is exceeded by all three test series, but the fatigue strength of the butt joints made by LBPF and hot rolling vary significantly. This is thought to be related to differences between the AM and conventional joints in microstructure, static strength, residual stress level, and small crack-like defects that partially interact with stress concentrations at the weld transition.