The presence of odontoblasts in dentinal tubules has been noted by numerous investigators. One of the first to describe their presence was B. Orban (l Dent Res 20:553-557, 1941). The cells were in tubules associated with forceps marks on the root surfaces. Odontoblasts in dentinal tubules have been reported due to various types of trauma to pulps. Cavity preparation (V. E. JAMES and I. SCHOUR, J Dent Res 34:698-699, 1955; LEFKOWITZ ET AL, Ohio State Dent Soc J 31:24-27, 1957; E. A. MARSLAND and D. S. SHOVELTON, Brit Dent J 102: 213-222, 1957; D. S. SHOVELTON and E. A. MARSLAND, Brit Dent J 105:16-26, 1958; K. LANGELAND, Odont T 65:249-386, 1957; J. J. KENNEDY ET AL, Dent Prog 3:10-16, 1962; M. BRANNSTROM, Odont Rev 14:224-253, 1963; and others) elicited this response when the cavities were cut dry or when severe trauma was provoked. Others such as I. R. H. Kramer and J. W. McLean (Brit Dent J 92:255-261, 1952), 0. Muller and B. Maeglin (Int Dent J 4:167, 1953), and K. Langeland (Odont T 68:463472, 1960) found this response under various filling materials, especially in unlined cavities. H. R. Stanley and H. Swerdlow (JADA 58: 49-59, 1959) related the appearance of aspirated odontoblasts to the degree of inflammation. Most investigators agree that this occurs soon after the toxic stimulus is applied, as noted in specimens collected 24 hours after treatment. M. B. Weiss, M. Massler, and J. M. Spence (Dent Prog 4:10-19, 1963) also found that extraction of teeth may cause this response. Thus squeezing of the pulp may force the odontoblasts into the dentinal tubules. The present observations on the teeth of mature mice prepared for electron microscopy in a study of the normal development pattern of dentin included study of the ultrastructural appearance of odontoblasts in the tubules in some of the teeth that had been extracted with forceps. The teeth were fixed in 1.33% osmic acid in s-collidine buffer at 4 C for two hours, dehydrated through graded alcohols and propylene oxide and embedded in Epon 812, sectioned at 500 to 800 A, and viewed on an Hitachi 8 electron microscope. In Figure 1 an elongated odontoblast is seen in the upper left within a tubule located partly in the predentin (lower left) and partly in the more highly calcified dentin (upper right). This electron micrograph shows the presence of the organelles within the cell as well as the dark stained
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