Introduction: Paying attention to the cultural background of universities as one of the influential factors in hidden curriculum leads to the academic and social success of students. It is necessary to be aware of the diverse and numerous cultural needs of students in order to making decisions and cultural planning in the university. This study was conducted with the aim of determining the cultural needs of students of Shahid Beheshti school of nursing and midwifery in Rasht. Method: This is a descriptive analytical cross- sectional study that was conducted on 237 students of the Shahid Beheshti school nursing and midwifery in Rasht in 2022. The research tool was a researcher-made questionnaire with a 5-point Likert scale with 47 items and seven subscales. Data were analyzed using descriptive statistics methods including frequency, mean and standard deviation and Spiroman, Shapiro-Wilk, Mann-Whitney U, Friedman and Bonferroni correction tests through SPSS. Results:The results showed that 137 students (57.8%) were female, 211 students (89%) not working, 152 students (63.7%) native and 226 students (%95.4) single and average age of students was 21.3±3.6 years. The students’ culturally related needs in order of priority; development of sports activities, artistic skills, the need related to social customs and traditions, the development of social skills, spiritual matters, the need related to political thinking and religious matters. The results showed that there is no statistically significant difference between students' cultural needs and gender (p<0.005). Conclusion: According to the results of this study, Students have diverse cultural needs. From the viewpoints` of students, the need to develop sports and artistic activities is in priority. Therefore, it is recommended that the seven needs identified in this study should be taken into consideration by the university officials and to make appropriate planning should be done to meet these needs.