Tehran University Research Articles

The Effectiveness of Metacognitive Therapy on Depression, Emotion Regulation, and Self-concept in Students with Social Anxiety Disorder: A Quasi-Experimental Study

Background: Students with social anxiety disorder (SAD) face unique challenges that can significantly impact their academic performance, social interactions, and overall well-being. Objectives: This study aimed to explore the impact of metacognitive therapy (MCT) on depression, emotion regulation, and self-concept among students diagnosed with SAD. Methods: The study employed a quasi-experimental research design with a pre-test-post-test format and a control group. The statistical population consisted of students diagnosed with SAD at the University of Tehran in 2022. A total of 36 students with SAD were selected for the research sample through purposive sampling and were divided into experimental (n = 18) and control (n = 18) groups. The experimental group participated in ten 90-minute sessions of MCT, while the control group did not receive any intervention. Data collection involved administering the Liebowitz Social Anxiety Scale, Beck Depression Inventory, Emotion Regulation Questionnaire, and Beck Self-concept Test. The collected data were analyzed using multivariate analysis of covariance (MANCOVA) in SPSS-24. Results: The results indicated that Metacognitive Therapy (MCT) significantly decreased depression (F = 50.65, P = 0.001, η² = 0.63) and significantly increased cognitive reappraisal (F = 61.81, P = 0.001, η² = 0.67), expressive suppression (F = 34.72, P = 0.001, η² = 0.54), and self-concept (F = 48.53, P = 0.001, η² = 0.62) in students with SAD. Conclusions: The findings of this study enhance our understanding of the therapeutic benefits of MCT for students struggling with SAD. By addressing critical psychological areas such as depression, emotion regulation, and self-concept, MCT shows promise as a valuable intervention for improving the well-being and functioning of students dealing with SAD.

The Assessment of Effectiveness of Anesthesiology Rotation in Acquiring Proficiency in Cardiopulmonary and Cerebral Resuscitation for Medical Students at Tehran University of Medical Sciences in the Years 2021-2022

Background: Whether in a hospital setting or in the community, medical students are exposed to cardiac arrests. Fast response and effective resuscitation are main determinants for decreasing mortality and morbidity due to cardiac arrests. We assessed the effectiveness of anesthesiology rotation in acquiring cardiopulmonary and cerebral resuscitation for medical students. Methods: A pre-test/post-test quasi experimental design was undertaken, comprising A fortuitious sample of medical students who were undergoing anesthesiology rotation at Tehran University of Medical Sciences hospital complexes during the academic year 2021-2022 using a self-administered questionnaire to evaluate their theoretical comprehension, knowledge, skills and before and after the rotation. Results: The study revealed an increase in theoretical scores (mean dif +2.6/20), increased in self-assessment knowledge scores (mean dif + 0.84/5), as well as an increased in self-assessment skill scores (mean dif +0.96/5). The highest scored were witness in students with previous cardiopulmonary resuscitation (CPR) experience. Conclusion: It is of extreme importance for medical students to participate in anesthesiology rotations. It is essential for possessing the appropriate knowledge and skills to provide effective resuscitation care and gain confidence to perform CPR.

Evaluating the Decision-Making Proficiency Among Medical Residents at Tehran University of Medical Sciences in the Year 2022-2023: A Descriptive Cross-Sectional Study

Background: To evaluate the decision-making proficiency among medical residents at Tehran University of Medical Sciences in the year 2022-2023. Methods: A structured online web-survey via national approved services Author’s designed questionnaire was used to collect the relative data based on variables of the study and was developed by the authors of the study by reviewing the previously conducted studies. The forms were sent to the medical residents at Tehran University of Medical Sciences as a link via electronic mail and social media; assistance was offered via direct or indirect contact upon request. Results: In this study, 88 medical residents of Tehran University of Medical Sciences were evaluated. Out of 88 participants, the frequency of females was 52(59.09%) and frequency of males was found out to be 36(40.91%). The frequency of the first- year residents was 28(37.50), second year residents was 33(21.59), third year residents was 19(9.09) and the fourth- year residents was found out to be 8(31.82). The frequency of different specialties were: Pathology 1(1.14%), Infectious diseases 1(1.14%), Cardio vascular diseases 1(1.14%), Emergency medicine 1(1.14%), Orthopedics2(2.27%), Psychiatry 3(3.41%), ENT 12(13.64%), Internal Medicine 13(14.77%), Pediatrics13(14.77%), OB&GYN 19(21.59%), Anesthesiology19(21.59%), Dermatology 2(2.27%), General Surgery 1(1.14%). Conclusion: There is a significant relationship based on linear regression between not having self - reported availability bias and surgical residency specialties. The surgical specialty is less prone to the availability bias. A difference of communication exists between the surgical and nonsurgical speciality and the nonsurgical specialties need to confirm their decision using other methods to prevent the patient harm.

Investigating the relationship between missed/rationed nursing care and organizational commitment in Iranian nurses

Background and objectiveThe primary objective of the nursing profession is to provide comprehensive and appropriate nursing care that meets the individual needs of patients. However, instances of missed/rationed nursing care can jeopardize the delivery of complete and safe healthcare, potentially putting patients’ lives at risk. The level of organizational commitment demonstrated by nurses is likely to impact various personnel and organizational factors. Therefore, this study aims to predict instances of missed/rationed nursing care by examining the influence of organizational commitment.MethodThis descriptive and cross-sectional study will be conducted in 2023. Three hundred nurses working in general and intensive critical care units at Tehran University of Medical Sciences hospitals were randomly selected. Data collection included Allen and Mayer’s organizational commitment questionnaires, Kalish’s missed care questionnaire, and demographic variables. A multiple linear regression model was used to analyze the prediction of missed care by commitment and other variables. The relationship between these variables was also explored using SPSS version 26 software.FindingsHalf of the nurses reported occasionally missing nursing care. Moreover, more than half of the nurses reported moderate organizational commitment in all dimensions. The most significant reasons identified by nurses for missed care were financial resources, human resources, and communication (p < 0.001). There was a significant relationship between missed/rationed nursing care and organizational commitment (p = 0.042). In the multiple regression equation, a significant portion of missed care due to commitment was predicted when considering demographic variables (p < 0.001).ConclusionBy understanding the relationship between organizational commitment and missed care, and identifying the factors contributing to missed/rationed care, managers can improve the efficiency of human resources and allocate appropriate financial resources. Establishing effective communication with employees can also enhance their commitment to addressing neglected care, ultimately reducing instances of oversight.

The relationship between nurses’ professional commitment and missed nursing care: a cross-sectional study in Iran

Background & aimsMissed nursing care is a global challenge that can have many consequences and it’s known as a common threat to the patient safety and quality of nursing care. The nurses’ professional commitment plays the final role in the occurrence of this issue. This study aimed to investigate the relationship between nurses’ professional commitment and missed nursing care in Iran.Materials & methodsThis cross-sectional descriptive-analytical study was conducted on 270 nurses working in two hospitals affiliated to Tehran University of Medical Sciences (Sina & Shariati) from September to December 2023. The research community included all nurses working in medical-surgical wards in the designated hospitals. The research samples were included in a targeted and quota method after obtaining organizational permits, ethical approval and informed consent. Data collection was done using three questionnaires including demographic information form, Nursing Professional Commitment Scale, and MISSCARE Questionnaire. The collected data was analyzed in SPSS software, version 25.ResultsThe results of Pearson’s correlation test showed that there is a significant negative relationship between nurses’ professional commitment and missed nursing care scores (r=-0.23) (p < 0.001). The mean score of nurses’ professional commitment was reported at a moderate to high level (83.72 ± 16.02) and the mean score of missed nursing care was reported at a low level (47.84 ± 16.48). Nurses over 40 years old, morning shift and employed nurses showed higher professional commitment. Also, nurses under 29 years old and male nurses had more missed nursing care other than their participants. No relationship was observed between other demographic variables with nurses’ professional commitment and missed nursing care.ConclusionThe results of this study showed that higher nurses’ professional commitment leads to higher quality of care and less missed nursing care. Based on findings of this research, it is recommended for health care providers and nursing managers to adopt programs to increase nurses’ professional commitment and consequently reduce missed nursing care.

False Negative Rate of Sentinel Lymph Node Biopsy (SLNB) in Breast Cancer Patients after Neoadjuvant Chemotherapy

Background: Managing the axilla in patients with node-positive breast cancer who converted to node-negative patients after neoadjuvant chemotherapy is a challenging issue. We aim to analyze the false negative rate (FNR) of SLNB after neoadjuvant chemotherapy in this group of patients. Methods: In this cross-sectional study, we reviewed the results of SLNB and ALND in 368 breast cancer patients who underwent neoadjuvant chemotherapy from 2015 to 2019. The study included patients with pathologically proven axillary lymph nodes undergoing NAC. We collected the data from the Breast Diseases Research Center of Tehran University of Medical Sciences. Results: The average age of the patients was 46.58±10.91. Of all the patients, 205 (55.7%) had positive SLN in the histologic analysis after surgery, while 163 (44.3%) had negative results. The study also revealed that the FNR of SLNB was 9.8% (n=16). Our results showed that SLN had 86.55% sensitivity and 100% specificity in detecting involved nodes. Furthermore, after multivariable analysis, we observed that a higher number of ALND dissections were associated with a higher FNR (Odds ratio; OR=1.20, 95% CI: 1.02-1.42); while a higher number of SLN excisions was linked with a lower FNR (OR=0.41; 95% CI: 0.17-0.98). Conclusion: After NAC in breast cancer patients with positive lymph nodes, SLNB is feasible with a low FNR; the latter is correlated with the number of nodes removed during the procedure.

Enhancing E-learning in higher education: Lessons learned from the pandemic

This study delves into the intricate realm of evaluating and refining e-learning practices, drawing upon insights from esteemed higher education professionals. Through a qualitative lens rooted in phenomenology, the research engages in semi-structured interviews with 30 faculty members from seven public universities in Tehran, ensuring theoretical saturation. Employing inductive content analysis, the data examination unveils three core categories of challenges: educational assessment, research evaluation, and university services. Emphasizing the significance of internal task performance, adaptability to dynamic environments, and external institutional promotion, the study advocates for the development of a comprehensive evaluation model and tools to uphold program quality, efficiency, and effectiveness. Against the backdrop of the COVID-19 pandemic, the research highlights the pressing need for Iranian higher education to bolster productivity through strategic resource allocation across education, research, services, and human capital development domains. Such efforts are pivotal in shaping a more prosperous future and bolstering societal scientific and cultural standing.

Introduction and content analysis of "Nurse's voice" WhatsApp group during COVID-19 pandemic: a qualitative study

BackgroundAt the beginning of the COVID-19 pandemic in Iran, the School of Nursing and Midwifery of Tehran University of Medical Sciences created several joint virtual groups to connect with people and answer their questions. One of these groups was called "Nurse's Voice,” in which nursing professors answered people's questions. The purpose of the current study was to analyze the content of questions in this WhatsApp group during the COVID-19 pandemic.MethodThis retrospective media content analysis qualitatively analyzed the text of questions and answers extracted from the above-named WhatsApp group.ResultsOut of 629 questions asked, 364 questions (58.0%) were related to COVID-19 and 265 questions (42.0%) were not. Those related to COVID-19 concerned COVID-19 symptoms and diagnosis, prevention and care, fear and worry, and interpretation of laboratory tests. The questions related to prevention and care fell into four subcategories: basic health principles, prevention of COVID-19 infection, care during COVID-19 infection, and care during COVID-19 recovery.ConclusionsNursing and midwifery schools can take advantage of the scientific capacity of professors and students for virtual communication and social accountability by integrating telehealth into their curricula. Cyberspace provides an opportunity for people to ask questions anonymously. With the results of the current study, the educational needs of the people during possible future epidemics can be predicted and answered faster. Furthermore, the large number of questions unrelated to COVID-19 indicates the need for counseling and training of the general public by nurses in all situations.

COMPARATIVE ANTI-TRICHOMONAS VAGINALIS ACTIVITY EVALUATION OF SAMBUCUS NIGRA L. FLOWERS AND FRUITS EXTRACTS

Background: Sambucus nigra or also known as Black elderberry or European elderberry is been used for the teatment and prevention of various diseases. The plant posseses health beneficial effects such as antimicrobial, antioxidant, antiulcerogenic, antidepressant, antidiabetic, antiviral and antiinflammatory. However, there is limited report on the antitrichomoniasis activity of S. nigra. Objectives: The study aimed to comparatively analyze the in vitro trichomonicidal effects of various extracts prepared from both fruits and flowers of S. nigra. Half maximal inhibitory concentration (IC50) and minimum parasiticide (MPC) values of the extracts against metronidazole-resistant Trichomonas vaginalis 50143 strain were determined by the liquid microdilution method using 96-well microplates. Results: After 48 hrs incubation with parasites, the highest activities were observed for 70% hydroethanolic extract, chloroform extract and methanol extract of fruits with IC50 values of 513, 531 and 566 µg/ml, respectively. Fruit extracts exhibited higher activity than flower extracts, and no activity was observed in methanol extract of flowers. Conclusion: This study is the first comparative analysis of the anti-Trichomonas vaginalis activity of elderberry extracts. This study will serve as a reference source in isolating the components of elderberry fruits with antiparasitic activity. Peer Review History: Received 23 March 2024; Revised 6 May 2024; Accepted 27Metronidazole June; Available online 15 July 2024 Academic Editor: Dr. Amany Mohamed Alboghdadly, Ibn Sina National College for Medical Studies in Jeddah, Saudi Arabia, amanyalboghdadly@gmail.com Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my Dr. George Zhu, Tehran University of Medical Sciences, Tehran, Iran, sansan4240732@163.com

PERIPHERAL BLOOD COUNT RECOVERY TIME COURSE DURING INDUCTION TREATMENT FOR ACUTE LYMPHOBLASTIC LEUKEMIA IN CHILDREN

Background: In recent years, the survival rate of pediatric ALL patients has increased to almost 90%, particularly for individuals with favorable prognoses. This success is mostly attributable to the use of risk-adapted medication, enhanced supportive care, and adjustments to therapy based on each patient's unique pharmacodynamics and pharmacogenomics. This research investigates ALL patients' response to induction chemotherapy, focusing on changes in peripheral blood cell counts and hematological markers, to predict therapeutic outcomes and provide a practical prognostic parameter. Methods: This study involved 100 under 15-year-old patients with newly diagnosed ALL treated in pediatric leukemia units at Kuwait Hospital, Sana'a. Blood markers were collected and analyzed using Epi Info statistical program version 6. The study followed the French, American, and British classifications of pediatric leukemia. Participants were informed of the study's goals and advantages, and their participation was optional. Results: The majority of patients were aged 5-9 years, with a mean age of 6.9 years. Hemoglobin levels were measured before and after treatment, with the mean level being 8.8 mg/dL before and 10.8 mg/dL after treatment. Severe anemia was reported in 10% of patients before treatment and decreased to 2% after treatment. PCV levels were also measured before and after treatment, with the mean PCV level being below normal in 66% of patients before induction chemotherapy but improving to 20% after treatment. The mean RBC count before treatment was 2.9 cells × 106/μl, but improved to 3.8 cells × 106/μl after induction chemotherapy. The study found that over 50% of patients had iron deficiency anemia before and after induction chemotherapy, with a mean neutrophil percentage of 18.2%, lymphocyte percentage of 73.7%, monocyte percentage of 3.8%, and eosinophil percentage of 0.85%. Conclusions: The study reveals that blood cell parameters replenish at different rates during induction chemotherapy, resulting in rapid platelet and slow neutrophil recovery. Changes in blood markers during induction may be prognostic. Peer Review History: Received 6 April 2024; Revised 10 May 2024; Accepted 25 June; Available online 15 July 2024 Academic Editor: Dr. Asia Selman Abdullah, Pharmacy institute, University of Basrah, Iraq, asia_abdullah65@yahoo.com Average Peer review marks at initial stage: 6.5/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Dr. A.A. Mgbahurike, University of Port Harcourt, Nigeria, amaka_mgbahurike@yahoo.com Dr. George Zhu, Tehran University of Medical Sciences, Tehran, Iran, sansan4240732@163.com

EVALUATION OF ANTIBIOTIC SENSITIVITY OF ODONTOGENIC BACTERIA IN CERVICOFACIAL CELLULITIS IN THE CITY OF SANGMELIMA, CAMEROON

Aim and objective: Cervicofacial cellulitis of dental origin is a polymicrobial infection characterized by inflammation of the deep spaces of the neck and face. These conditions can be life-threatening, as the bacteria can spread throughout the body, leading to descending necrotizing mediastinitis, sepsis and death. Management is greatly challenging because of microbial polymorphism, probabilistic antibiotic selection treatment sometimes being hesitant and inappropriate because of lack of consensus. The aim of this study was to evaluate the antibiotic susceptibility of odontogenic bacteria in cervicofacial infections. Material and methods: This was a cross-sectional, descriptive study conducted from May 2019 to August 2020 in the odontostomatology and bacteriology departments of the Sangmelima District and Reference Hospitals in Cameroon. Thirty five samples were collected by swabbing or puncture. Specific culture media were used to inoculate samples. Sensitivity of isolated bacteria was assessed using the Kirby-Bauer diffusion disk method. Data analysis was performed using SPSS 23.0. Results: Thirty samples were positive (85.7%). Bacteria isolated were Streptococcus (40%), Staphylococcus (30%), Pseudomonas (20%), Escherichia coli (6.7%) and Lactobacillus (3.3%). Sensitivity testing was performed with amoxicillin/clavulanic acid, metronidazole, gentamicin, ciprofloxacin, imipenem and clindamycin. Bacteria were more sensitive to amoxicillin/clavulanic acid, ciprofloxacin and imipenem. Conclusion: Bacteria identified in purulent secretions from cervicofacial cellulitis are much more sensitive to amoxicillin/clavulanic acid, ciprofloxacin and imipenem. Peer Review History: Received 25 March 2024; Revised 4 May 2024; Accepted 27 June; Available online 15 July 2024 Academic Editor: Prof. Dr. Gorkem Dulger, Duzce University, Turkey, gorkemdulger@yandex.com Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Dr. George Zhu, Tehran University of Medical Sciences, Tehran, Iran, sansan4240732@163.com Prof. Hassan A.H. Al-Shamahy, Sana'a University, Yemen, shmahe@yemen.net.ye

Metabolic syndrome in personnel of TUMS☆ and its association with types of occupations: A cross-sectional study

BackgroundMetabolic syndrome (MetS) causes an increased risk of cardiovascular disease and death. Hospital staff as an influential segment of the population are at risk of developing MetS. This study aimed to determine the occupational distribution of MetS in hospital staff and to examine the association between the type of occupation and the likelihood of developing MetS. MethodsIn this cross-sectional study we recruited 3128 personnel of TUMS, including healthcare workers, office workers and supportive workers of Tehran's University Teaching Hospitals during 2020–2022. Demographic, anthropometric indices and laboratory tests were recorded. MetS was determined using three definitions including ATP III recommendations, IDF and regional IDF criteria. Statistical analysis was performed using SPSS 26. ResultsMetS was observed in 641 (20.5%) subjects according to ATP III recommendations, 923 (29.5%) based on IDF and 713 (22.8%) using regional IDF criteria. Using regional IDF criteria, age, gender and occupational status were associated with the MetS occurrence (all p < 0.0001). Adjusting for the age and gender of participants, healthcare workers were less likely to develop MetS compared to both office workers (OR = 0.66, 95%CI for OR: 0.55–0.80, p < 0.0001) and supportive workers (OR = 0.72, 95%CI for OR: 0.58–0.90, p = .003). However, office workers and supportive workers did not differ significantly regarding this parameter (p = .44). ConclusionRegardless of the MetS definition criteria, the findings indicated an alarming MetS prevalence, particularly among supportive workers and office workers. Considering the significant role of hospital staff in patients’ health promotion, effective management is essential.

Histopathological, morphological, and molecular characterization of fish-borne zoonotic parasite Eustrongylides Excisus infecting Northern pike (Esox lucius) in Iran

Eustrongylides excisus is a fish-borne zoonotic parasite known to infect various fish species, including Northern pike (Esox Lucius). This nematode, belonging to the family Dioctophymatidae, has a complex life cycle involving multiple hosts. This study aimed to investigate the occurrence of Eustrongylides nematodes in Northern pike (E. Lucius) collected from Mijran Dam (Ramsar, Iran). Between June and October 2023, an investigation was conducted on Northern pike from Mijran Dam in Ramsar, Iran, following reports of reddish parasites in their muscle tissues. Sixty fish were examined at the University of Tehran, revealing live parasites in the muscles, which were then analyzed microscopically and preserved for a multidisciplinary study. The skeletal muscle tissues of 85% (51/60) of fish specimens were infected by grossly visible larvae which were microscopically identified as Eustrongylides spp. In histopathological examination, the lesion was composed of encapsulated parasitic granulomatous myositis. Microscopically, the cystic parasitic granulomas compressed the adjacent muscle fibers, leading to their atrophy and Zenker’s necrosis. Moreover, epithelioid macrophages, giant cells and mononuclear inflammatory cells were present around the larvae and between the muscle fibers. Finally, a molecular analysis by examining the ITS gene region, revealed that they belong to the species E. excisus. Eustrongylidiasis in northern Iran necessitates further research into the biology, epidemiology, and control of Eustrongylides nematodes, focusing on various hosts. This study is the first to comprehensively characterize E. excisus in Northern pike in Ramsar, Iran, raising concerns about possible zoonotic transmission.

Designing a Temporary Camp for Accommodation After an Earthquake in a Multi-purpose Open Space Located in Shahid Beheshti University in Tehran, Iran

Background and objective The current study focuses on the planning, management and design of a temporary accommodation camp as well as the design of a temporary shelter. The main goal is to present the physical model of a temporary accommodation camp in the football field of Shahid Beheshti University, Tehran, Iran, to be used after an earthquake, considering the physical and non-physical conditions of the district 1 and the existing infrastructure. Method This is a descriptive-analytical survey study. Qualitative method and interviews with five experts, field observation, documents, and articles were used for collecting information. Results Based on the findings of observations, interviews and the library information, the suitable space for the establishment of the accommodation complex in the university was identified and predicted, based on this, the description of the duties of the established bodies was stated. Then, according to the design priorities, temporary accommodation was designed and the production arrangements were determined to reduce the risk. Conclusion The results give an insight into the construction projects related to temporary accommodation and transportation in post-disaster conditions in Tehran, considering the economic, environmental and psychological conditions of the residents, and issues such as innovative locating, flexibility, coordinated planning and reusability and the use of social capital.

“I know how much he cares for our learning”: Moral Life in University ELT Classrooms

In Iran’s higher education English language teaching (ELT) context, we explored the moral nuances embedded in two conversation courses offered to undergraduate students majoring in English Literature at one of the branches of the Islamic Azad University (IAU) in Tehran. We collected data through classroom observations, field notes, and semi-structured individual and focus group interviews. Our content analysis revealed a range of moral values embedded in significant incidents, which we categorized into five themes: (a) relations, (b) rules and regulations, (c) assessment, (d) curricular substructure, and (I) culture. These themes were further subcategorized into more specific concepts that emerged from our data. We selectively focused on two subthemes, Class Initiation and Turn Taking, as well as the main theme of Assessment, as these were the most morally loaded, with a higher frequency of moral values extracted from related class episodes compared to other categories. This selection allowed for a deeper exploration of the complexities and nuances within these themes, providing further support on the role of ELT teachers as moral exemplars for their students.

The Effect of Random Biopsy and Endo-Cervical Curettage in Diagnosis of Precancerous Cervical Lesions in Women With Normal Colposcopy.

This study aimed to evaluate the effect of random biopsy and endo-cervical curettage (ECC), alone and together, in the diagnosis of high-grade cervical lesions in women with normal colposcopy. This cross-sectional study was conducted on 356 women who underwent colposcopy examination in the gynecology-oncology clinic of Roointan-Arash Women's Hospital, affiliated with Tehran University of Medical Sciences. All eligible women underwent colposcopy. In colposcopy examination, up to four biopsy samples were randomly taken for each quartile of the cervix and ECC was performed. Finally, the rate of intraepithelial neoplasia (CIN) and the relation between random biopsy and ECC and CIN diagnosis was determined. In total, 27 (7.6%) low-grade squamous intraepithelial lesions (LSIL) and 19 (5.3%) high-grade squamous intraepithelial lesions (HSIL) were detected in cases that underwent random biopsy. There was a significant association between the abnormal random biopsy results and having multiple sexual partners (p=0.001), cigarette (p=0.041), and hookah (p=0.033) smoking. Furthermore, 31 (8.7%) LSIL and 42 (11.8%) HSIL were reported in women who underwent ECC. There was a significant relation between the abnormal results of ECC and hookah consumption (p=0.008) and human papillomavirus (HPV) infection (p=0.011). The concordance (p=0.001) between random biopsy and ECC result was 71.2% and only in 28.8% of the cases one of the methods was normal and the other was abnormal. The present study showed that the simultaneous use of ECC and random biopsy in women with normal colposcopy increases the detection rate of precancerous lesions by up to 28.8%.

The Covid-19 Infection Effect on Assisted Reproductive Method Outcomes.

COVID-19 can have potential pathogenic effects on the oocyte and embryos, but there is limited data about its impact. This study aimed to investigate the COVID-19 impact on the outcome of Assisted Reproduction Techniques (ART) methods. This case-control study was conducted on 190 infertile women who underwent oocyte retrieval at Yas Hospital in vitro fertilization (IVF) department affiliated to Tehran University of Medical Sciences, from October 2021 to October 2022. The case group was defined as women whose PCR test was positive on puncture day and the control group was women with COVID-19 negative tests on puncture day. The study outcome measurements included the number of oocytes retrieved and the number and quality of embryos. Finally, the data were analyzed by SPSS 24. The mean age of the participants was 32.89 ± 5.58 years with an age range of 18-49 years. No significant difference was observed between the two groups regarding baseline variables. The mean number of oocytes was significantly (p =0.001) lower in the case (6.68±4.25) group versus the control (9.07±4.10) group. While there was no statistically significant difference regarding the mean number of embryos in the study groups, No grade A embryos were observed in more than half (57.5%) of the women in the case group. Furthermore, the frequency of grade C embryos on average was 1.08±1.11 in the case group and 0.57±0.75 in the control group, with a statistically significant difference (p =0.010). The findings of this research highlighted that women infected with COVID-19 on the puncture day have a lower number of oocytes and also good-quality embryos.

Designing a Multiple-Epitope Vaccine Candidate against Leishmania major and Leishmania infantum for Monocyte-Derived Exosome Preparation.

Leishmania is a vector-borne protozoon, which causes visceral, cutaneous and mucocutaneous leishmaniosis in human and animals. Monocyte-derived exosome vaccines can be used as prophylaxis and immunotherapy strategies. The aim of this study was to design a multiple-epitope candidate vaccine using leishmaniolysin (GP63) and rK39 proteins against Leishmania major and L. infantum for monocyte-derived exosome preparation. This study was carried out in Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, 2023-2024. Effective immunodominant epitopes were selected from two antigenic proteins of GP63 and rK39 using various immunoinformatics and bioinformatics approaches. Vibrio cholerae β-subunit was used as an adjuvant to stimulate immune responses. Then, appropriate linkers were selected for the fusion of epitopes. The 3D model of candidate vaccine was predicted and validated. This designed candidate vaccine could effectively be used as a prophylaxis strategy against leishmaniosis. A candidate vaccine was designed using bioinformatic and immunoinformatic studies with virtual acceptable quality; however, effectiveness of this vaccine should be verified through further in-vitro and in-vivo studies.

Evaluation of Nanonanoliposomal Curcumin on Cutaneous Leishmaniasis Skin Lesions Caused by Leishmania major in BALB/c Mice.

Curcumin is an extract of rhizome turmeric (diferuloylmethane), with antioxidant, anti-inflammatory, antimicrobial, and anti-parasitic properties, which making it a potential candidate for the treatment of leishmaniasis. The aim of the presented study was to evaluate curcumin as possible candidate for treatment of cutaneous leishmaniasis. We investigated the physicochemical properties and anti-leishmanial effects of nanoliposomal curcumin (40, 80, and 120 μM) in Leishmania major (MRHO/IR/75/ER) infected BALB/c mice at the faculty of Veterinary Medicinem University of Tehran, Iran. For this aim, L. major promastigotes (MHROM/IR/75/ER) at stationary phase (2×106) were inoculated sub-cutaneously into the upper area of the tail in BALB/c mice (six groups, n= 10 per group). For evaluation of nanoliposomal curcumin, the zeta potential, particle size and stability of nanoliposomal curcumin was determined. Furthermore, the anti-leishmanial effects of nanoliposomal curcumin formulation on the lesion sizes was determined and the parasite burden in the leishmania induced lesion was performed using semi quantitative PCR. Treatment of L. major infected BALB/c mice with nanoliposomal curcumin led to a reduction in the kinetic of the skin lesion size development. The semi quantitative PCR analysis of DNA extracted from the lesions showed reduction of parasite burden. The most effective treatment could be found in 80 μM nanoliposomal curcumin. Treatment with Glucantime, as a positive control, also showed a nearly similar effect compared to the effect of 80 μM nanoliposomal curcumin. Nanoliposomal curcumin could be considered as a potential drug against cutaneous leishmaniasis caused by L. major in susceptible animal models.

Genetic Diversity and Phylogenetic Study of Leishmania Species in Iran by Multilocus Sequence Typing.

Leishmaniasis is an important public health parasitic infection, which is endemic in many parts of the world, including Iran. We aimed to investigate genetic diversity and phylogenetic relationship among different Leishmania isolates using multi-locus sequence typing (MLST). Totally, 41 isolates collected either from patients referred to Leishmaniasis Diagnostics and Treatment Center at Tehran University of Medical Sciences, Tehran, Iran or from animals during 2019-2021, were subjected to the study. They included L. major and L. tropica from human, L. infantum from canine, and L. turanica from rodents from different endemic foci of Iran analyzed using MLST including gp63, g6pdh, lack, nagt, and hsp70 genes. A total of 5010 bps was analyzed from each isolate. The three targets, nagt, lack, and g6pdh, generated better topology comparing to the other genes. In the 44 isolates, 22 haplotypes (STs) were identified. Leishmania tropica contained the highest number of haplotypes (n=12) comparing to L. major (n=8), L. infantum (n=1) and L. turanica (n=1). All five genomic loci caused separation of Iranian Leishmania species at the species level, indicating conservation of these genes in the Leishmania parasite. The highest number of haplotypes belonged to L. tropica, indicating that the genetic diversity of this species is higher than that of L. major. It was further confirmed that the MLST is a suitable method to examine genetic variation of Leishmania parasites with respect to evolutionary and epidemiological studies.