Abstract An innovative procedure is introduced for the identification of the mechanical parameters of orthotropic plates of arbitrary shape, under various boundary conditions, based on vibration data. The method employs a combination of a convenient Rayleigh-Ritz approach and Particle-Swarm Optimization to estimate elastic constants of the orthotropic material in a straightforward manner, without requiring computationally demanding iterative Finite Element analyses. Specifically, the pb-2 Rayleigh-Ritz procedure is extended and applied to deal with orthotropic plates, simplifying the approach to more easily treat generic plate shapes, taking advantage of the Green's theorem. The method is then appropriately combined with the Particle-Swarm Optimization procedure to expeditiously identify material parameters based on available vibration data. Several numerical applications are presented to show the reliability of the approach, and comparisons with pertinent results available in the literature demonstrate the efficiency and accuracy of the proposed procedure. The study is then supplemented by experimental tests developed in the Laboratory of Experimental Dynamics at the University of Palermo, Italy. In this context, because of the obvious relevance for modern additive manufacturing processes, vibration tests are performed on several 3D printed stiffened plates. Numerical vis-a-vis experimental data are examined, showing that the proposed procedure accurately capture equivalent orthotropic parameters of the stiffened plates.