Universitas Padjadjaran Research Articles

Graduate income and profession linkage: Tracer study of public relations graduates

Background: Higher educational institutions must prepare graduates for careers by monitoring their performance. They must also evaluate academic relevancy to practical needs. The PR program at Universitas Padjadjaran strives to deliver the best education and monitor graduates’ performance. Purpose: This paper aims to study how graduates’ first income is associated with GPA, study duration, and job wait time. This study also linked public relations education with graduates’ current position in their work based on their self-assessment. Methods: Using tracer study data from the Career and Development Centre of Universitas Padjadjaran, exploratory data analysis was done to examine the characteristics of graduates based on the observed variables. The bivariate analysis investigated the association between income, GPA, study duration, and job wait time. Conclusion: Findings of this study show no statistical evidence of the relationship between first salary, GPA, and duration of the study. Exploratory data analysis shows that PR program graduates perform well when comparing the average income of undergraduates on a national level. Graduates’ self-assessment of study relevancy indicated that most graduates possess the relevant educational background and appropriate level of education for their current occupational position. As the statistical testing for this study was conducted post hoc, careful considerations need to be taken when taking the results at face value, considering potential flaws in the sampling frame and the power of the tests. Implications: The findings in this study would serve as a platform for future references on graduates or tracer study, more specifically public relations graduates, as it provides a starting point for measuring graduates’ performances. As the database provides more data with more graduates in future years, an updated finding of this particular study would be very important to measure how well graduates fare post-graduation.

Nursing Students’ Practicum Readiness Using Virtual Learning Reality: A Pre-Experimental One Group Study

Technology can be utilized in various fields, with no exception of nursing education in which virtual reality is used as an educational tool. However, the use of virtual reality as a learning media for nursing is still limited, especially as an introductory tool to practicum sessions. This study aimed to give a description on the use of virtual reality as a learning media towards the readiness of students in Faculty of Nursing, Universitas Padjadjaran. A pre-experimental one-group post-test only design was used. A total of 68 nursing students participated and completed a virtual reality suctioning simulation for 20 minutes. The data were analysed using a descriptive analysis consisting scores of minimums, maximums, mean, and standard deviation. The means and standard deviation for practicum readiness were 16.84 and 6.899 respectively. The minimum score acquired in total was 6 out of a possible correct answer of 45, with the maximum score acquired being 34. Based on the result, virtual reality as a learning media has effects on practicum readiness. Further studies should take the implementation of a comparison group and the number of opportunities for participants to do the simulation procedure into consideration so that practicum readiness is more comprehensively described

Stress Levels and Sleep Disorders among Undergraduate Nursing Students During COVID-19 Pandemic: A Cross-Sectional Study from Indonesia

The COVID-19 pandemic has forced universities to change their teaching and learning activities using online methods, which can impact students' stress problems, including undergraduate nursing students. This study aims to identify the relationship between stress levels and sleep disorders among nursing students in online learning during the COVID-19 pandemic. A cross-sectional study included 253 nursing students from the Faculty of Nursing at Universitas Padjadjaran between 2018 and 2021, and completed the Student Nurse Stress Index (SNSI) questionnaire and the Pittsburgh Sleep Quality Index (PSQI) questionnaire from June 2022 to July 2022. Study findings, all individuals reported experiencing moderate to severe stress levels and had an impact on sleep disorders, with a sig. (2-tailed) namely 0.001 (<0.05) and correlation (r) 0.290. The majority reported moderate stress, i.e. less disturbed sleep (70.6%), disturbed (63.2%), and very disturbed (52.6%). Meanwhile, severe stress, namely disturbed sleep (33%) and very disturbed (47.4%). Although, there were 14.3% had mild stress and slept less disturbed. The highest levels of stress and sleep disorders were reported by women (85.4%), 17-21 years old (78.7%), and lived with their family (77.1%). This study highlights that the COVID-19 pandemic related to stress and sleep disorders has a significant relationship. Therefore, future studies using meta-regression are needed to investigate the factors influencing and exacerbating stress and sleep disorder among nursing students in online learning during the COVID-19 pandemic to minimize results bias.

Rancangan program alternatif metrik pada Pusat Pengelolaan Pengetahuan Universitas Padjadjaran

Padjadjaran University Knowledge Management Center has rebuilt its website by adding the GLAM Repository element. The website plans to install alternative metric tools: PlumX which is found in a subscription package repository called Digital Commons. This research aims to create a program design based on the four substances of information system management: technology, databases, procedures, and human resources. This study uses a qualitative approach with method action research. The data collection process is carried out using interviews, observation, literature studies, documentation, and focus group discussions. Data are gathered from librarians and staff through interviews and FGDs. Data analysis techniques conducted by researchers included: data reduction, data presentation, and data conclusion. Results researched shows that in the field of technology and databases, the website was rebuilt through the addition of GLAM and repository elements. Knowledge Management Center unify databases between faculty libraries. Alternative metric procedures has been successfully submitted to Universitas Padjadjaran. The librarians need to learn about alternative metrics. The outputs of alternative metric implementation program designs are proposals, draft terms of reference, and Digital Common and Plumx learning materials. The conclusion of the research is that the design of alternative metric programs requires technological support, databases, updating of computer units, and staff training in managing alternative metrics.

Pengetahuan pengguna tentang kematangan karir ditinjau dari kualitas informasi pada Instagram @glintsid

Glints Indonesia is one of the media that is commonly used as an ecosystem for career maturity, career development, and online-based job recruitment. Not only active through the website, but Glints Indonesia is also active through the Instagram platform with the username @glintsid. The information contained in @glintsid's post suggests new knowledge to its users. This study aims to determine user knowledge about career maturity in terms of the quality of Instagram @glintsid on career tips content. The research method used in this research is qualitative.descriptive Data collection techniques used is observation and interviews with 5 (five) respondents who are final year students of Universitas Padjadjaran who are also Instagram followers @glintsid. The data analysis method used in this research includes data reduction, data presentation, and conclusion. The results of this study indicate that @glintsid's Instagram posts have fairly information quality with accurate, relevant, timely, and completeness indicator. Respondents found that the information content of @glintsid's posts is easy to understand, relevant to needs, timeliness, and clear enough to avoid ambiguity in seeking career tips. In terms of user knowledge indicators, respondents considered that the presence of career tips content on Instagram @glintsid had increased their knowledge through fulfilling indicators such as career planning, career exploration, knowledge about decision making, a world of work information, knowledge of preferred occupation group, and knowledge about the realization of career decisions. The study results show that the quality of information on Instagram @glintsid is quite good and can add new knowledge to respondents.

Hubungan antara kemampuan literasi digital dengan pencegahan berita hoaks di kalangan mahasiswa

One of the impacts of the development of information and communication technology is the internet, which allows us to create and receive information from anywhere and at any time. This can have bad consequences because not all information on the internet can be accounted for. The purpose of this study is to determine how much the relationship between digital literacy skills consisting of internet searching skills, hypertextual navigation, content evaluation, and knowledge assembly with the prevention of hoax news in students of the Faculty of Communication Sciences, Padjadjaran University. The approach used in this research is a quantitative correlational approach. Data collection techniques were carried out by distributing questionnaires online and a literature study. Respondents in this study were active students of class 2017, 2018, and 2019 of the Faculty of Communication Sciences, Padjadjaran University, as many as 95 people. The sampling technique in this study used proportional stratified random sampling (random and stratified sampling). The results of this study show a significant relationship between students' digital literacy skills consisting of internet searching, hypertextual navigation, content evaluation, and knowledge assembly with the ability to prevent hoaxes in students of the Faculty of Communication Sciences, Padjadjaran University. This research is expected to add insight into the field of digital literacy and hoax prevention and can be a reference for further research, especially for those who will examine digital literacy at the Faculty of Communication Sciences, Universitas Padjadjaran.

Sustainable Development Goals (SDGs), Digital Education and Digital School

Purpose: Promoting digital education, the SDG Fund is convening partnerships between UN Agencies, governments and the telecommunications industry to better use information technologies to advance SDG4. Affordable, reliable and context-sensitive digital education, can promote equal opportunities for girls and boys and reduce inequalities by ensuring every child has access to high quality content. Digital education technologies improves fundamental skills such as collaboration, problem solving and global awareness.
 Design/methodology/approach: Sustainable Development Goals (SDGs), Digital Education Documents by funding sponsor, Compare the document counts for up to 15 funding sponsors, first European Commission, followed by National Natural Science Foundation of China, and Horizon 2020 Framework Programme. Documents by affiliation, Compare the document counts for up to 15 affiliations, first University College London, followed by University of Oxford and University of Washington. Subject area Documents, first Social Sciences, followed by Environmental Science and Computer Science. Documents by country or territory, Compare the document counts for up to 15 countries/territories, first United States, followed by United Kingdom and Spain. In ASIA, first India and China. In ASEAN, first Malaysia, followed by Indonesia, Singapore, Thailand, Philippines, and Viet Nam. From Indonesia, Documents by funding sponsor, Compare the document counts for up to 15 funding sponsors, first Direktorat Jenderal Pendidikan Tinggi, followed by Universitas Airlangga, Universitas Indonesia and Universitas Padjadjaran. Documents by affiliation, Compare the document counts for up to 15 affiliations, first Universitas Indonesia and Universitas Sebelas Maret. Sustainable Development Goals (SDGs), Digital Education, Documents by funding sponsor, Compare the document counts for up to 15 funding sponsors, first National Natural Science Foundation of China, European Commission, and Horizon 2020 Framework Programme.
 Findings: Documents per year by source, first Sustainability Switzerland journal, Journal Of Cleaner Production, and Remote Sensing journal. Documents by affiliation, Compare the document counts for up to 15 affiliations, first University of Washington, Columbia University, and London School of Hygiene. Documents by country or territory, Compare the document counts for up to 15 countries/territories, first United Kingdom, United States, and Australia, in ASIA, first China, India, and Japan. In ASEAN first Malaysia, Singapore, Thailand, Indonesia, Viet Nam, Philippines, and Cambodia. In Indonesia, Documents by funding sponsor, Compare the document counts for up to 15 funding sponsors, first Binus University, Direktorat Jenderal Pendidikan Tinggi, Lembaga Pengelola Dana Pendidikan, Universitas Airlangga, Universitas Indonesia, Universitas Katolik Parahyangan, and Universitas Sebelas Maret. Documents by affiliation, Compare the document counts for up to 15 affiliations, first Universitas Indonesia and Universitas Sebelas Maret. Universitas Indonesia and Universitas Sebelas Maret are dominant in Indonesia for Sustainable Development Goals (SDGs), Digital Education, Digital Education, research publication.
 Paper type: Research paper

DINAMIKA PERILAKU KONSUMEN MINUMAN KOPI DI MASA PANDEMI PADA KALANGAN MAHASISWA (Suatu Kasus Pada Mahasiswa Angkatan 2018 Program Studi Agribisnis Fakultas Pertanian Universitas Padjadjaran)

Drinking coffee has now become a lifestyle among college students. There are various types of coffee drink products that can be consumed, such as coffee shop-processed drinks and instant coffee. Since December 2019, there has been an outbreak of the Covid 19 disease and the issuance of a Large-Scale Social Restriction, leading to changes in consumer behavior in buying coffee drink products. The purpose of this study was to find out how the consumer behavior of coffee drink products before and during the pandemic among college students, and whether there were changes in consumer behavior of coffee drink products before and during the pandemic among college students. This research uses descriptive analysis with a survey method. The study was conducted on students of the Agribusiness Major, Faculty of Agriculture, Universitas Padjadjaran, with a sample of 100 people. The data analysis technique used is descriptive analysis. The results showed: (1) Prior to the pandemic, the majority of students received information from social media, received funds from their parents, consumed coffee shop-processed drinks, consumed it at the coffee shops, made purchases using the offline method, paid by cash, consuming it at a coffee shop at night, evaluating the product, and evaluate the product directly (2) During the pandemic period, the majority of students get information sources from social media, get funds from their parents, consume coffee shop-processed drinks, consume it at the coffee shops, make purchases using the offline method, make payments using e-wallet, consuming it at home in the afternoon, and not evaluating the product. (3) There are changes in some consumer behavior of coffee drink products among students.

Evaluation of Indonesian Butterfly Pea (Clitoria ternatea L.) Using Stability Analysis and Sustainability Index

Yield and yield attributes are important components in genotypic evaluation. The butterfly pea is a native plant of Indonesia, and it is considered an underutilized crop. The goals of this study were to evaluate genotypes using environment (year) interactions (GEIs) with yield and yield attributes, and evaluate butterfly pea genotypes based on stability measurements and sustainability index (SI). The study was conducted at the Ciparanje Experimental Field, Faculty of Agriculture, Universitas Padjadjaran using 35 butterfly pea genotypes in a randomized complete block design with two replications. The field trial was conducted over three years (2018–2020). The results showed that the yield and yield attributes were influenced by GEIs. Additive main effects and multiplicative interaction (AMMI) selected 11 stable genotypes (31.43%); genotype plus genotype by environment interaction (GGE) biplot, AMMI stability value (ASV), and genotype stability index (GSI), each selected six genotypes (17.14%) that were stable and high-yielding, and SI selected 18 genotypes (51.43%) that were stable and high-yielding. There were three genotypes identified by all measurements, namely G2, G14, and G16. These three genotypes can be selected as the superior genotypes of the butterfly pea for flower production, and can be used as material for crosses in plant-breeding prog.

The influence of hedonic values and extraversion on online impulse buying: Empirical evidence from Indonesia

The purpose of this study is to analyze Indonesian consumer hedonic values, extraversion, and online impulse buying, as well as to examine the influence of consumer hedonic values and extraversion personality on impulsive online buying. This study uses a quantitative research method that employs descriptive and associative tools. The primary data were gathered through social media surveys of Indonesian consumers who buy goods through e-commerce. Of the 440 respondents who received questionnaires, only 400 completed them accurately. According to the survey, at least 75% of respondents shop online regularly. The study’s findings describe three types of Indonesian online consumers: those with hedonic values, those with reasonably high extrovert personalities, and those prone to online impulse buying. Path analysis results indicate that both hedonic value and extraversion have a significant influence on online impulse buying. Hedonic consumers enjoy online shopping, and as a result, they discover items they had not previously considered purchasing without careful consideration. On the other hand, extroverted consumers who are outgoing, passionate, and pleasant in social situations are more likely to be interested in impulsive online buying. These results provide online business owners with necessary guidance by demonstrating the importance of developing a website that is not only informative but also visually appealing and engaging to trigger impulse buying. Acknowledgment This study was supported by a research grant from Universitas Padjadjaran, Indonesia.

Perkembangan, Dinamika, dan Tren Penelitian Jurnalisme di Indonesia Periode 2001-2020

After the authoritarian New Order regime collapsed, the study of journalism as a part of communication studies is projected will develop and helps rehabilitating the ecosystem of Indonesian media. However, there is no meta-analysis study that can prove how the development and dynamics of journalism research in Indonesia, especially after more than two decades of the collapse of the New Order. This study, by analyzing 641 journalism studies articles scattered in authoritative scientific publications on communication in Indonesia, in the span of two decades from January 2001 to December 2020, found that Indonesian journalism scholars were enriching literature reference, and were increasingly connected with other scholars. The findings obtained by this study directly refute many accusations that communication studies, especially in the scope of journalism studies in Indonesia, is dominated by positivistic paradigm. During the 2001- 2020 period, although there was an increase in publications that relied on a quantitative approach, it can be seen the dominance of the qualitative approach has not been deterred. The Ministry of Communication and Information Technology of Indonesia in the second position, as the institution with the highest number of scientific article writers after the Universitas Islam Bandung in first place, even surpassing Universitas Padjadjaran and Universitas Diponegoro shows that researchers from government institutions are one of the agents of production and reproduction of journalism knowledge and discourse in Indonesia. Based on keyword trends, the deliberative vision of journalistic research in Indonesia tends not to develop progressively.
 Keywords: Indonesia, journalism studies, meta-analysis, journalism researc

Differences in mandibular morphology between bruxism and non-bruxism patients based on the Levandoski analysis

Bruxism is a condition that involves grinding and clenching which can place a large load on the mandible, so it can change the morphology of the mandible. Based on the Levandoski analysis, this study aimed to determine whether there are differences in the mandibular morphology between bruxism and non-bruxism patients. This study was a cross-sectional analytic study conducted on the panoramic radiographic samples of bruxism and non-bruxism patients with a total sample of 30 patients (n = 30) in each group, obtained from the radiographic archives at the Radiology Installation of RSGM Universitas Padjadjaran which have been confirmed as bruxism and non-bruxism patients. Measurements were carried out using ImageJ software based on the reference lines by Levandoski. The results of the study were analyzed using SPSS software by performing normality and homogeneity tests, followed by a statistically independent t-test or Mann-Whitney test. The results of the independent t-test showed that three lines had a p-value < 0.05, namely the line from the point gonion to the maxillary vertical line on the right side, the distance from the top point of the condyle to the maxillary interincisal point on the right side, and the distance from the top point of the condyle to the mandibular interincisal point on the right side. The Mann-Whitney test results showed that two lines had a p-value < 0.05, namely the maxillary vertical midline and the distance from the condyle to the left maxillary vertical line. The other line had a p-value> 0.05. There were differences in the mandibular morphology between bruxism and non-bruxism patients based on the Levandoski analysis, namely the length of the maxillary vertical midline, the distance from the condyle top point to the maxillary vertical midline, the distance from the point gonion to the maxillary vertical midline, the distance from the top point of the condyle to the maxillary interincisal point on the left side, and the distance from the top point of the condyle to the mandibular interincisal point on the left side. On the other four lines, there was no significant difference between bruxism and non-bruxism patients.

Validation and Reliability of Lifestyle Instruments for Indonesian Adolescents with Hypertension Family History: A Rasch Model

BACKGROUND: There are lifestyle changes among adolescents in this modern era, such as less physical activities, because many activities can be carried out using modern technology, unhealthy food habits such as eating junk food, canned food and beverages, and preserved fruits and vegetables, poor sleeping habits, stress, and smoking behavior. These lifestyles are harmful and lead to cardiometabolic diseases such as obesity and hypertension, especially in adolescents with hypertension history in the family. AIM: This study aimed to assess the validation and reliability of healthy lifestyle instruments for Indonesian adolescents with a family history of hypertension. METHODS: This study is a survey research conducted from July 2021 to February 2022 in South Sumatera, Indonesia. The subjects were adolescents with a family history of hypertension and recruited using purposive random sampling. Adolescents aged 10–18 years old who returned signed parental consent forms, gave written assent to participate, and completed the questionnaire were included in the study. The questionnaire was consisted of three sections, Section I contained internal factors, Section II related to external factors, and Section III related to healthy lifestyle. The study was approved by the Research Ethics Committee Universitas Padjadjaran Bandung. Winsteps software was used to analyze the validity, reliability, unidimentionality, and rating scale. RESULTS: Obtained 103 valid question items with OUTFIT values 0.5 < mean square < 1.5; −2.0 < ZSTD < 2.0; and the point measure correlation (Pt Mean Corr) value is in the range of 0.32 < Pt Measure Corr < 0.85. The reliability value of respondents and instruments is 0.88, with a ’Cronbach’s alpha value of 0.95. The raw variance explained by the measured value is 72.3%, and all unexplained variance values are <15%. CONCLUSIONS: Healthy lifestyle instruments are valid and reliable, so it is feasible to measure the healthy lifestyle of Indonesian adolescents with a family history of hypertension. This instrument can measure the success of a program or intervention in changing a lifestyle in adolescents at risk of developing hypertension, especially adolescents with a family history of hypertension. We suggest further research involving more participants in a broader area. We hope the healthy lifestyle instruments can be used in a standardized manner for all regions of Indonesia.

Changes of Soil Nutrient Content as Influenced by Water Hyacinth Compost and Inorganic Fertilizers on Shallot

One of the problems with Inceptisols is their low level of soil fertility. The addition of one or more available nutrients can help the soil become more fertile and enhance quality of plants. The goal of this experiment was to find out the optimum ratio of water hyacinth compost, N, P, K, and S fertilizers in relation to variations in the soil’s nutrient content, quality and yield of shallot Batu Ijo Variety. This research was conducted from February to August 2020, at the Laboratory of Soil Chemistry and Plant Nutrition Field Experiments, Universitas Padjadjaran. The research design used in this study was a randomized block design (RBD) with seven treatments, which were: one control treatment, two recommended dosage treatments of N, P, K, S fertilizers, and four combination treatments of water hyacinth compost and N, P, K, and S fertilizers. The findings demonstrated that the treatment of 1 dose recommendation of compost plus ¾ dose recommendation of N, P, K, and S was able to increase soil nutrient content and quantity (number of shallot bulbs). The use of this experiment is as information to optimize the use of water hyacinth compost in efforts to increase the productivity of soil and shallot plants.

The effect of Trichoderma-enriched biofertilizer application on total nitrogen, organic carbon, and peanut (Arachis hypogaea L.) yield in Inceptisols

The addition of N, P, and K nutrients through the application of fertilizer that is not in accordance with recommendation dosage still happening nowadays. One of the efforts to minimize this problem is the use of biofertilizer. This research aimed to study the effect of Trichoderma-enriched biofertilizer (T-EB) application on total nitrogen, organic carbon, and peanut (Arachis hypogaea L.) yield in Inceptisols. The research was conducted at The Laboratory of Soil Chemistry and Plant Nutrition, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor from May to October 2022. The design used was randomized block design (RBD) with nine treatments and three replications: (A)Control, (B)NPK Standard, (C) ½(T-EB)+1NPK, (D) ¾ (T-EB) + 1NPK, (E) 1(T-EB) + 1NPK, (F)1½(T-EB) + 1NPK, (G)1(T-EB) + ¾NPK, (H)1(T-EB) + ½NPK, (I)1(T-EB) + ¼ NPK. The results showed that the use of Trichoderma-enriched biofertilizer had a significant effect on soil total nitrogen, soil organic carbon, and peanut yield components compared to the control (without fertilizer) and NPK standard. A single dose of (T-EB) (2 kg/ha) effectively increased peanut yield when mixed with the NPK dose of standard fertilizer at ¾ (37.5 kg/ha). The use of (T-EB) at that dose can also reduce NPK usage in Inceptisols.

Effect of NPK, and Si Fertilizers on Exchangeable Potassium, K Uptake and Growth of Black Rice (Oryza sativa L. indica) on Ultisols

Ultisols are one of the soil orders that dominate dry land in Indonesia, and they have a lot of fertility issues. NPK and Si fertilization is used to enhance soil conditions and deliver nutrients to plants. Jeliteng cultivar black rice is a type of rice that can be cultivated on dry land. This research was conducted from October 2021 to January 2022 at the Experimental Land for Soil Chemistry and Plant Nutrition, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, West Java with an altitude of 750 meters above sea level. The experimental design was carried out using a Randomized Block Design with nine treatments and three replications. The results showed that the combination of 1 NPK + 1 Silica fertilizer was the best treatment in increasing the exchangeable potassium of the soil, which was 0.70 cmol.kg-1 and K uptake was 1.91 mg/tanaman. There is no one best treatment dose for plant growth, but the addition of NPK, and Si fertilizers, can increase plant growth, specifically plant height and number of tillers.

Dental age estimation in Indonesian adults: An investigation of the maxillary canine pulp-to-tooth volume ratio using cone-beam computed tomography.

This study was performed to develop a linear regression model using the pulp-to-tooth volume ratio (PTVR) ratio of the maxillary canine, assessed through cone-beam computed tomography (CBCT) images, to predict chronological age (CA) in Indonesian adults. A sample of 99 maxillary canines was collected from patients between 20 and 49.99 years old. These samples were obtained from CBCT scans taken at the Universitas Padjadjaran Dental Hospital in Indonesia between 2018 and 2022. Pulp volume (PV) and tooth volume (TV) were measured using ITK-SNAP, while PTVR was calculated from the PV/TV ratio. Using RStudio, a linear regression was performed to predict CA using PTVR. Additionally, correlation and observer agreement were assessed. The PTVR method demonstrated excellent reproducibility, and a significant correlation was found between the PTVR of the maxillary canine and CA (r=-0.74, P<0.01). The linear regression analysis showed an R2 of 0.58, a root mean square error of 5.85, and a mean absolute error of 4.31. Linear regression using the PTVR can be effectively applied to predict CA in Indonesian adults between 20 and 49.99 years of age. As models of this type can be population-specific, recalibration for each population is encouraged. Additionally, future research should explore the use of other teeth, such as molars.

Reference service user’ satisfaction at the Animal Husbandry Faculty Library, Universitas Padjadjaran

Reference services satisfaction assessment in libraries are one of the factors that determine the satisfaction of library users. Therefore, periodic improvement and development of reference services is required. This study was aimed to determine the extent of user satisfaction with reference services that have been implemented in the Library of the Faculty of Animal Husbandry, Universitas Padjadjaran. This study employed a quantitative descriptive approach based on survey to 132 respondents representing each academic year. The survey data were analyzed descriptively and presented in graphs. The questions in the survey cover four topics, namely the ease of borrowing and returning procedures, staff performance, reference information access, and library facilities. The results showed that respondents answered satisfied with a consistent level across the topics measured above. Even so, improvements are needed, especially in the aspect of library facilities, which are marked by responses with low scores. Service improvement in these four aspects can generally be focused on changing the paradigm from "library-centered" to "user-centered" by accommodating the expectations and desires of most users. In addition, after conducting training on access to reference collections, respondents answered that the training was considered very useful and was expected to be carried out periodically. In conclusion, users are satisfied with the reference service at the Faculty of Animal Husbandry Library, Universitas Padjadjaran. In addition, the training program on access to library collections has been successfully implemented and felt very useful by respondents and can be categorized as a service improvement activity.

The Effect of Water Extract of Salam Koja Leaf (Murraya Koenigii (L.) Spreng) Against Root-Knot Nematode (Meloidogyne Spp.) in Tomato Plants

Root-knot nematodes (Meloidogyne spp.) is one of the pathogens that attack tomato plants so that it can reduce tomato production. One alternative to control Meloidogyne spp. environmentally friendly is to use botanical nematicides. Salam Koja (Murraya koenigii (L.) Spreng) has potential as a botanical nematicide because its leaves contain secondary metabolites which are antihelmintic. The purpose of this study was to determine the effect of water extract of M. koenigii leaves and to obtain the best concentration in suppressing the attack of Meloidogyne spp. on tomato plants. This research was conducted at the Greenhouse, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor. The study used an experimental method with a randomized block design consisting of 6 treatments and 4 replications. The treatments included control (without water extract of M. koenigii leaves), concentrations of water extract of M. koenigii leaves 6%, 9%, 12%, 15%, and carbofuran 2 g/plant. The results showed that the water extract of M. koenigii leaves had an effect on suppressing root-knot nematodes (Meloidogyne spp.) on tomato plants. The water extract of M. koenigii leaves at the highest concentration of 15% is effective in suppression of the number of galls in the roots (38.57%) and suppression of the number of juvenile 2 (J2) Meloidogyne spp. in 100 ml of soil (81.03%). Murraya koenogii leaf water extract can be used to control Meloidogyne spp. in tomato plants.

Application of Replicate Organism Detection and Counting Method (RODAC) in Measuring Mycobacterium Tuberculosis Contamination in High Burden Laboratories

Background: Technicians working in high burden tuberculosis (TB) laboratories pose a higher risk of being infected by Mycobacterium tuberculosis from clinical samples. Contamination control is mandatory to detect the release of bacteria into the working environment and to minimize the risk of exposure to the workers. The contamination measurement is rarely performed due to the lack of standard methodology. This study optimized and applied a unique culture-based method named Replicate Organism Detection and Counting (RODAC) plates to assess the presence of M. tuberculosis contaminant in the laboratory with high burden of clinical samples. Methods: RODAC was applied on twenty working surfaces in the Mycobacteriology Laboratory of Universitas Padjadjaran. The results of RODAC were compared with DNA-based detection from the same working surfaces using in-house IS6110 real-time PCR (IS6110-qPCR). The detection limit of the RODAC plate was 19.6 CFU mL-1.Results: From all working surfaces tested, two distinct colonies were found on RODAC plate stamped on the Ziehl-Neelsen staining basin. Those colonies were identified as M. tuberculosis and non-tuberculous mycobacteria (NTM), as confirmed by the MPT64 antigen test and the presence of acid-fast bacilli. IS6110-qPCR detected the presence of M. tuberculosis DNA in ten sampling points, including the ZN staining basin, incubators, and microscopy areas. IS6110-qPCR detected more working surface contamination versus RODAC. However, it was noted that RODAC, which was a culture-based method, detected live bacteria, while PCR could not distinguish between live and dead bacteria.Conclusion: The application of the RODAC plate is more suitable for monitoring the contamination of live bacteria in the working environment and to inform a proper corrective action.