Universitas Padjadjaran Research Articles

Enhancing Broccoli growth by bacillus rhizosphere bacteria

This study examines the enhancement of broccoli growth by Bacillus Rhizosphere. Bacteria Broccoli is a nutrient-rich vegetable known for its high content of polyphenols, flavonoids, vitamins, minerals, fiber, and low calorie count, making it a recommended daily food. Tropical regions grow broccoli in mountainous areas. Sustainable agriculture emphasizes minimizing chemical fertilizers, and biofertilizers like Plant Growth Promoting Rhizobacteria (PGPR), such as Bacillus, can reduce their use. Bacillus improves plant growth by fixing nitrogen, solubilizing phosphate and potassium, and producing phytohormones. Understanding Bacillus growth dynamics is vital for optimizing its agricultural application. This study aimed to analyze growth curves of different Bacillus species and investigate their potential role in promoting broccoli growth. This study was carried out in January–April 2024 at the Soil Biology Laboratory, Faculty of Agriculture, Universitas Padjadjaran, and Bumi Agro Technology Company, Lembang. The research was divided into 3 stages: growth curve determination, Bacillus liquid inoculant preparation, and application of treatment to plants. The findings showed that isolates of B. safensis strain MDL5, B. altitudinis strain RPW2, B. subtilis strain YPS4, and Bacillus sp. strain SZ057 reached the highest point in their growth curves after 4 and 5 days of incubation. After that, the curves slowly went down. Inoculating Bacillus into broccoli plants resulted in an increase in plant height by 43.28–58.75% compared to the control.

Effectiveness of Short-Term Use Denosumab and Risedronate Using β-Crosslaps and Histopathology as a Parameter in Osteoporotic Rat Model

Osteoporosis is a condition of decreased bone mass density. Pharmacological management uses drugs that decrease bone resorption or increase bone formation. The most commonly used drugs are bisphosphonates. Risedronate is one of these bisphosphonates. It inhibits osteoclasts, resulting in osteoclast apoptosis. Denosumab is a human monoclonal antibody that inhibits receptor activator kappa-B ligand, inhibiting osteoclast activation. Both drugs are widely used. Research on the efficacy of these two mechanisms has yet to obtain conclusive results. β-cross-laps is a parameter that can be used to evaluate the effectiveness of therapy through monitoring the bone resorption process. This experimental study used female rats >9 weeks old and was conducted at the Pharmacology and Therapeutic Laboratory, Universitas Padjadjaran Bandung, Indonesia, from June to September 2021. This study utilized a simple random sampling to allocate 24 experimental animals into three groups: control, risedronate, and denosumab. β-Crosslaps expression values before ovariectomy, post ovariectomy before receiving medication, and post ovariectomy and receiving medication was recorded and statistically analyzed using the SPSS version 24.0. The analysis of 24 samples revealed a statistically significant decrease in the median value of β-Crosslaps after ovariectomy in the denosumab group (p=0.036) when compared to the control group, whereas the decrease in the risedronate group was not significant (p=0.687). Administration of denosumab in rat models is more effective in reducing bone resorption compared to risedronate.

Respons pertumbuhan tanaman secang (Caesalpinia sappan L.) terhadap dosis pupuk kandang sapi

Sappan wood (Caesalpinia sappan L.) is a plant that contains the compound brazilin, which is found in the pith of its stem. The pith of the sappan wood stem can be used as a raw material for traditional medicine or as a dye with red pigment. Currently, there are no procedures or guidelines for cultivating techniques for sappan wood plants. Each annual plant requires a certain amount of organic material at the beginning of planting to support its growth. The organic material commonly used is manure, one of which is cow manure. This research aims to determine the effect of cow manure dosage on the growth of sappan plants. The experiment was conducted from March 2023 to December 2023 at Ciparanje Research Center, Faculty of Agriculture, Universitas Padjadjaran. The method used is experimental by design Randomized Block Design consisting of three treatments namely, it was repeated six times: 1 kg cow manure/plant, 2 kg cow manure/plant, and 3 kg cow manure/plant. The experimental results showed that treatment with 2 kg cow manure/plant had the best effect on plant height at 5,6,7,8, and 9 months after treatment (MAT) and the number of leaves at 5 and 9 MAT. There has been no response in plant diameter to the dose of cow manure fertilizer

Balancing Academic and Personal Life: Challenges for Medical StudentsPersonal Life in Medical Students

Medical students are found to experience stress with a considerable impact because of the academic studies they are undertaking. High demands compared to non-medical students can interfere with other life domains, such as personal life. This interference can manifest in several ways, such as diminished time for social interactions and leisure activities. The purpose of this study was to explore and understand the potential impact on the relationship between academic life and personal life. The research was conducted with a sample of 7 medical student participants from Universitas Padjadjaran. Participants completed a questionnaire with 5 open-ended questions designed to capture their experiences and perspectives in detail. The findings revealed that a significant number of participants struggle with time management, feeling that their academic commitments leave them with minimal time for free activities and social engagements. This imbalance contributes to a sense of inadequacy in maintaining a fulfilling personal life. The study highlighted that a well-rounded personal life is crucial for sustaining optimal academic performance. A good personal life can provide good academic conditions, but the lack of free time for personal life makes participants do other alternatives such as postponing, procrastinating, and causing stress. Addressing these issues requires a holistic approach that not only alleviates academic burdens but also promotes a healthy balance between professional and personal domains. It’s essential for ensuring the well-being and success of medical students in both their academic pursuits and personal life.

Effects of application methods of microbial mixture on early blight disease (Alternaria solani) and growth of potato

Early blight (Alternaria solani) is one of important diseases in potato in Indonesia. In previous study, a microbial consortium (Trichoderma harzianum, Bacillus subtilis and Pseudomonas sp.) suppressed the bacterial wilt disease in potato. For application, the microbial consortium was mixed with compost (10%). As the microbial consortium is originally intended for controlling soil-borne diseases, this study examined the ability of the microbial consortium to suppress early blight disease and the effects of application methods on its efficacy. The application methods examined were the methods that targeted for soil-borne as well as airborne diseases. The experiment was conducted in a greenhouse at the Department of Plants and Diseases, Faculty of Agriculture, Universitas Padjadjaran, Sumedang, West Java, Indonesia. The experiment used Randomized Complete Block Design with 10 treatments and three replications. The treatments included application of microbes mixed with compost in planting hole solely or in combination with soaking the potato tubers in suspension of the microbes, drenching water extract of the mixture at 2, 4, 6 weeks after planting (WAP) with or without foliar application of the water extract. The result showed that application method influenced the abilities of the microbial mixture in suppressing early blight disease and supporting the potato growth. Even though the application of microbe-compost in planting medium inhibited the development of early blight disease by 37.8-39.5%, the regular foliar spray of the water extract of the mixture enhanced the disease suppression. Combination between application of the microbe-compost in the planting hole, drenching its water extract at 2, 4, 6 WAP and weekly foliar spray of the water extract suppressed early blight disease by 80.8%. This combination of application methods also supported better plant height, numbers of potato leaflets, roots fresh weight and production of potato tuber.

Cytotoxicity Effect of Aqueous Propolis Extract of Geniotrigona thoracica Sumatrans on Colo-201 Colon Cancer Cell Line and Senescence Colo-201 Colon Cancer Cell Line Induced by Low-dose Doxorubicin

Propolis, a resinous compound honeybees produce, demonstrates an extensive spectrum of powerful biological properties. However, the anti-cancer activity of propolis derived from Geniotrigona thoracica Sumatrans has yet to be reported. Thus, we sought to investigate the cytotoxicity of aqueous propolis extracts from Geniotrigona thoracica Sumatrans against Colo-201 colon cancer cell line and senescence Colo-201 colon cancer cell line induced by low-dose doxorubicin. This study was conducted at the Parasitology Laboratory of Human, Safety, and Environment, Universitas Padjadjaran Bandung from January to May 2024. This study assessed cell viability using the WST-1 test. Non-induced Colo-201 cells were treated with an aqueous extract of propolis (AEP) 100 ppm, or 5-fluorouracil (5-FU) 5 mg/ml as the positive control or water as a vehicle on untreated control. Colo-201 senescence was induced by doxorubicin 0.1 µM for three days. Doxorubicin-induced Colo-201 senescence was then treated with AEP 100 ppm, with 5-FU 5 mg/ml as the positive control, or with the combination of AEP 100 ppm and 5-FU 5 mg/ml, or water as a vehicle on untreated control. The data were analyzed using SPSS version 25.0, a one-way ANOVA, and Tukey’s post hoc test. The results showed that AEP has cancer-killing effects on Colo-201 cells and Colo-201 senescent cells induced by low-dose doxorubicin. AEP-treated Colo-201 cells and Colo-201 senescent cells induced by low-dose doxorubicin viability were significantly reduced to 37.15% and 13.72%, respectively, although slightly higher than those of the 5-FU-treated one at this concentration. There was also a decrease in the cancer-killing effect of 5-FU from 88.55% in non-induced Colo-201 cells to 41.5% in the doxorubicin-induced Colo-201 senescence model. In conclusion, aqueous extract of propolis from Geniotrigona thoracica Sumatrans showed cancer-killing-effects both on the Colo-201 colon cancer cell line and senescence Colo-201 colon cancer cell line induced by low-dose doxorubicin.

Influence of phosphate and boron addition to mixed liquid fertilizer on the growth and yield of red chili cultivated in the subsoil layer

This study assesses the impact of adding phosphate and boron in a mixed liquid fertilizer (MLF) on the growth and yield of red chili plants cultivated in a subsoil environment. The experiment was conducted to comprehend how these nutrients affect the performance of red chili plants, particularly when cultivated in less ideal conditions such as subsoil, a remnant of excavation for brick production. The research was carried out at the Ciparanje Garden of the Faculty of Agriculture, Universitas Padjadjaran. The research design employed a Randomized Completely Block Design with the following treatments: A = Control (degraded soil without fertilizer), B = 0% MLF + 1 NPK, C = 0.25% MLF + 1 NPK, D = 0.50% MLF + 1 NPK, E = 0.75% MLF + 1 NPK, F = 1.00% MLF + 1 NPK, G = 0.5% MLF + 3/4 NPK, H = 0.5% MLF + 1/2 NPK, I = 0.5% MLF + 1/4 NPK, J = 0.5% MLF + 0 NPK, and K = 1 NPK in normal soil. The results revealed that the addition of phosphate and boron in MLF significantly influenced the growth and yield of red chili in subsoil conditions. The recommended MLF concentration was 0.75%, alongside the standard NPK dose. These findings provide crucial insights for the development of more efficient and sustainable agricultural practices, especially in challenging soil conditions like subsoil, where nutrient availability can be a determining factor for agricultural success.

Evaluating the effect of plantlet age and Trichoderma spp. application on acclimatization of Boehmeria nivea L. Gaud

One of the crucial stages in the ramie plant (Boehmeria nivea L. Gaud) tissue culture is the acclimatization phase, which transitions between the in vitro and ex-vitro environments. Determining the appropriate age of plantlets and providing nutrients in the substrate play significant roles in the success of acclimatization. This research is conducted to determine the most suitable age of plantlets for acclimatization and assess the impact of providing nutrients through Trichoderma spp. application on the growth of ramie seedlings during the acclimatization phase. The experiment was conducted between January and March 2023 at the Screenhouse Bale Tatanen, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, Sumedang. The experimental design used was a Randomized Complete Block Design (RCBD) with two factors: plantlet age with three levels (4, 8, and 12 weeks old) and Trichoderma spp. fertilizer application with two levels (without and with Trichoderma) with 4 replications. The results showed that the 4-week-old plantlets exhibited the best height, root number, root length, and leaf number growth. Additionally, the application of Trichoderma spp. increased the stem diameter of the ramie seedlings during the acclimatization phase.

Effect of biochar composition and paclobutrazol concentration on growth and yield of G1 Medians cultivar potato seeds in Jatinangor medium plain

Potato seed production in the medium plain is necessary to avoid environmental damage and the limited area of potato seed production in the high plain. The effort to increase the production of G1 potato seeds on the medium plain is the evaluation of the biochar composition and concentration of paclobutrazol that can increase the production G1 potato seeds cv Medians in the medium plain of Jatinangor. The research was conducted from August to December 2022 at the Ciparanje Experimental Field. Jatinangor. Faculty of Agriculture. Universitas Padjadjaran. The experiment used a Factorial Randomized Block Design (RBD). The first factor was the biochar composition: m1 (100% compost), m2 (80% compost + 20% rice husk biochar), m3 (80% compost + 20% coconut shell biochar), and m4 (80% compost + 10% biochar husk rice + 10% coconut shell biochar). The second factor was the paclobutrazol concentration: p1 (without paclobutrazol), p2 (100 ppm paclobutrazol), and p3 (150 ppm paclobutrazol). The experimental results showed that there was no reaction effect of biochar compositions and paclobutrazol concentrations on the growth and yield of G1 potato seeds. The application of 80% compost + 20 % rice husk biochar gave significantly higher yields than treatment without biochar. The number of tubers was 6.29 knol per plant and the tuber weight was 66.80 g per plant. Application of 100 ppm paclobutrazol resulted in lower plant height, gave the number of tubers was 6.39 knol per plant, tuber weight per plant was 63.34 g higher than without paclobutrazol and produced more seed tubers with S class.

Seed Longevity of Soybean (Glycine max L.) Cultivars Under Lightless Storage

Soybean seeds in tropical countries are prone to rapid deterioration due to environmental condition during storage, leading to a decrease in seed quality and ultimately resulting in low potential yield. Longevity of seed during storage is influenced by genetic and environmental factors such as light. Therefore, this study aimed to determine the effect of light on the vigour of two soybean seeds cultivars (Demas 1 & Devon 1 cvs) stored for three months. The investigation was conducted from February to May 2022 at Seed Technology Laboratory, Faculty of Agriculture, Universitas Padjadjaran. A correlational method was employed to assess the relationship between seed quality of Demas 1 and Devon 1 in light and lightless storage. Furthermore, data were analyzed using correlation regression and t-test to compare the two populations. The results showed that there was a high correlation between the physical and physiological quality of seeds. There were also differences in seed response to light, and this was observed from the moisture content, weight of 100 seeds, germination rate, and simultaneous growth percentage traits. Considering the seed quality traits, Demas 1 had the best vigour value after storage in dark conditions. It is worth noting that both cultivars exhibited signs of deterioration during storage. However, the rate of deterioration was slowed down due to lightless condition.

HUBUNGAN LITERASI DIGITAL DENGAN PERILAKU KEAMANAN SIBER PADA MAHASISWA PERPUSTAKAAN DAN SAINS INFORMASI UNIVERSITAS PADJADJARAN

This research aims to find the relationship between digital literacy and cybersecurity behavior among Library and Information Science students in Universitas Padjadjaran. The concept of digital literacy used in this research is based on three dimensions, including technical, cognitive and social-emotional dimensions. This study used a quantitative method with correlational approach, that attempts to analyze the relationship between two or more variables and the strength of the relationship. 75 students from classes of 2020, 2021 and 2022 in the department have been selected as the target of this study with simple random sampling technique. The results of the research show that the three dimensions, namely technical, cognitive and socio-emotional dimensions, all are having a significant relationship with cybersecurity behavior. The three are also having a low level of relationship when it comes to cybersecurity behavior. Based on the results, it can be concluded that digital literacy has a significant relationship with cybersecurity behavior, despite the level of the relationship between the two are low. Which also means that a high level of digital literacy doesn’t guarantee good cybersecurity behavior either.

The effect of botanical pesticide application and mycorrhiza on colonization of mycorrhiza, soil microbial communities, growth and yield of maize on Ultisols

An experiment to study the effect of synthetic and botanical pesticide and arbuscular mycorrhizae (AMF) on mycorrhizal colonization, population of soil microbes, growth and yield of maize on Ultisols was carried out in green house of Agriculture Faculty, Universitas Padjadjaran at Jatinangor, Sumedang District, West Java Indonesia The experiment design used was Factorial Randomized Block, consisted two factors. The first factor was pesticide consisted five levels i.e. without pesticide; neem (Azadirachta indica A. Juss) pesticide; Annona muricata pesticide ; furadan and regent. The second factor was mycorrhiza consisted two levels i.e. without AMF inoculation and AMF inoculation. The experiment consisted two units with three replications; the first unit was harvested in the end of vegetative period to observe percentage of mycorrhizal infection, population of soil bacteria and fungi, fosfor uptake and dry weight of shoot, and the second unit was harvested in the end of generative period to find out the yield of maize. The results of this experiment showed that there was no interaction effect between pesticide and AMF on percentage of mycorrhizal infection, population of soil bacteria and fungi, fosfor uptake, dry weight of shoot and yield of maize. Pesticide decreased percentage of mycorrhizal infection and population of soil bacteria and increased yield of maize. Furthermore, AMF increased fosfor uptake and yield of maize.

COMBINATION OF ETHNOMATHEMATICS AND THE MOZART EFFECT TO IMPROVE PROBLEM-SOLVING SKILLS AND MATHEMATICAL DISPOSITION

The background of this research is that student learning outcomes in analytical geometry lecture during the transition from pandemic to Covid-19 endemic are still low, which is due to a lack of student interest in learning, and they are still accustomed to online learning, thus having an impact on their low problem-solving skills and mathematical disposition. This research aims to determine to what extent the implementation of ethnomathematics and the Mozart effect can improve students' problem-solving skills and mathematical disposition in analytical geometry lecture during the transition from pandemic to endemic COVID-19, so the research is important to do. The implementation of ethnomathematics and the Mozart effect in mathematics learning is unique because it is a combination of learning approaches that have never been used before in Indonesia and other countries. The research method used was a quasi-experimental non-equivalent control group design because this research was experimental and sample determination was not carried out randomly, but using purposive sampling technique on the second-semester students of the mathematics undergraduate program, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran. The instruments used in this study were problem-solving skills test, mathematical disposition scale, and students’ attitude questionnaire toward learning with the implementation of ethnomathematics and the Mozart effect. The results showed that: (1) problem-solving skills of students who received learning by implementing ethnomathematics and the Mozart effect are better than students who achieved direct instruction; (2) mathematical disposition of students who received learning by implementing ethnomathematics and the Mozart effect is better than students who achieved direct instruction; and (3) students are interested and motivated to learn mathematics by implementing ethnomathematics and the Mozart effect. This research concludes that the implementation of ethnomathematics and the Mozart effect can improve students' problem-solving skills and mathematical disposition in analytical geometry lecture during the transition period from the pandemic to endemic COVID-19. It can be seen from good average post test scores achieved by students.

The Level of Satisfaction Among Patients with Dental Bridges

Dental bridges are one of the treatments for tooth loss cases. Dental profession students at the Faculty of Dentistry of Universitas Padjadjaran (Unpad) conducted their first dental bridge treatment on patients. Evaluating patient satisfaction is essential to measure the success of treatments provided by dental students. This study aims to determine patient satisfaction with the dental bridges treatment provided by dental professional students at the Prosthodontics Polyclinic in terms of chewing, aesthetics and phonetics function, maintenance of the denture, and psychological aspects. This type of research is descriptive cross-sectional. Eighty-two dental bridges users were selected using total sampling at the Prosthodontic Polyclinic from September 2022 to February 2023 out of a total population of 86 users. The inclusion criteria included male or female patients using PFM or full metal dental bridges in the upper or lower jaw, worn for at least seven days to 6 months after definitive cementation, and made a control visit after the insertion of dental bridges. The instrument used was a modified questionnaire called the Patient Denture Assessment (PDA) and an Indonesian version of PDA (PDA-ID), with 12 modified Likert scale questions given online. The results of the study showed that 86,6% of the respondents were satisfied with masticatory function, 97,6% were satisfied with aesthetics and phonetics, 82,9% were satisfied with the maintenance of the denture, and 93,9% were satisfied with the psychological aspects of the use of dental bridges. Most patients were satisfied with dental bridges regarding chewing, aesthetics and phonetics function, denture maintenance, and psychological aspects.

Response of growth and tuber seed production of G0 potato (Solanum tuberosum L.) cv Medians in medium lands of Jatinangor to biochar composition and retardant type

Potatoes are the third most consumed food in Indonesia. The availability of high-quality potato tuber seeds is still low which affects potato production. Potato planting in the highlands is limited, so it needs to be expanded into medium lands. The modification that can increase the production of G0 potato tuber seeds on medium lands is the use of biochar and retardant. The purpose of this research was to determine the composition of biochar and the type of retardant to increase the G0 tuber seed yield of potato cv Medians in the medium lands of Jatinangor. The research was conducted from August 2022 to February 2023 at the Station Ciparanje, Jatinangor, Faculty of Agriculture, Universitas Padjadjaran. The experiment used Factorial Randomized Block Design (RBD). The first factor was the biochar compositions: b1 (100% compost), b2 (80% compost + 20% rice husk biochar), b3 (80% compost + 20% coconut shell biochar), and b4 (80% compost + 10% biochar husk rice + 10% coconut shell biochar). The second factor was the retardant type: r1 (without retardant), r2 (100 ppm paclobutrazol), and r3 (100 ppm prohexadione-Ca). The experimental results showed that there was no interaction effect of biochar compositions and retardant type on the growth and yield of G0 potato tuber seeds. Treatment of 80% compost + 20% coconut shell biochar suppressed plant height but increased the number of tubers. Treatment of 100 ppm paclobutrazol resulted in lower plant height but total chlorophyll content, percentage of stolon forming tubers, the number of tubers, and tuber weight per plant were higher compared to treatments without retardant and with 100 ppm prohexadione-Ca.

Growth and yield response of Watani Wado job's tears (Coix lacryma-jobi L.) to the application frequency of phosphate fertilizer and paclobutrazol in different growing seasons

Job's tears (Coix lacryma-jobi L.) is a carbohydrate source functional food crop that can be developed as a substitute for rice. This study aims to determine the best application frequency of phosphate (P) fertilizer and paclobutrazol for supporting the growth and yield of job's tears in both rainy and dry seasons. The research was conducted from November 2021 - November 2022 at the Ciparanje research station and Laboratory Centre Universitas Padjadjaran, Jatinangor, Sumedang. The experiment used a randomized block design (RBD) consisting of P fertilizer application frequency (once, twice, and thrice) and paclobutrazol application frequency (once, twice, and thrice) and three replications.. The best season was determined using the T-test. The results showed that there was no interaction between growth components and job's tears yield in both rainy and dry season planting. P fertilizer application had a significant effect on lateral branch per tiller in the rainy season and root-shoot ratio in the dry season. Paclobutrazol application had a significant effect on the number of tillers per plant and number of seeds per plant in the rainy season, and the lateral branch per tiller in the dry season. Planting in the rainy season had the best growth and yield components compared to the dry season.

Response of growth, yield, and seeds quality of G0 potato on different potassium fertilizer doses and retardant concentrations in Jatinangor medium land

This study aims to determine the best interaction of potassium (K) fertilizer dose and retardant concentration on the growth, yield, and seed quality of G0 potato grown in the medium land of Jantinangor. The experiment was conducted at Screen House Station Ciparanje, Jatinangor, Faculty of Agriculture Universitas Padjadjaran from November 2022 until January 2023 at an elevation of ± 685 meters above sea level. The experimental design used was the Factorial Randomized Block Design (RBD). The first factor was the K fertilizer dose including 50%, 100%, and 150%. The second was the retardant concentration including without retardant, 100 ppm paclobutrazol, 100 ppm prohexadione-Ca, and 150 ppm prohexadione-Ca. The experimental results showed that there was an interaction effect between the K dose and retardant concentration. The interaction effect of 150% K and 100 ppm prohexadione-Ca showed the highest percentage of L class seed tuber and the lowest tuber weight loss. Independently, 150% K improved growth rate, number of tubers (6.67 knol/plant), and weight of tubers (73.33 g/plant). Independently, 100 ppm paclobutrazol concentration increased the chlorophyll content index. 150 ppm prohexadione-Ca concentration suppressed leaf area and plant height but increased stomatal conductance, fast emergence shoot time, and shoot length. 100 ppm prohexadione-Ca concentration produced the highest number of tubers and the weight of tubers, i.e., 6.78 knol/plant and 74.33 g/plant, respectively.

Epidemiology of cleft lip and palate charity mission surgery at Bandung Cleft Lip and Palate Center, Indonesia: a 14-year institutional review.

The management of cleft lip and palate aims at improving the patient's aesthetic and functional outcomes. Delaying primary repair can disrupt the patient's functional status. Long-term follow-up is essential to evaluate the need for secondary repair or revision surgery. This article presents the epidemiology of cleft lip and palate, including comprehensive patient characteristics, the extent of delay, and secondary repair at our institutional center, the Bandung Cleft Lip and Palate Center, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia. This retrospective study aimed to determine the epidemiology and recurrence rates of cleft lip and palate at the Bandung Cleft Lip and Palate Center, Indonesia, from January 2007 to December 2021. The inclusion criteria were patients diagnosed with cleft lip and/or palate. Procedures such as labioplasty, palatoplasty, secondary lip and nasal repair, and alveolar bone grafting were performed, and data on recurrence were available. In total, there were 3,618 patients with cleft lip and palate, with an age range of 12 months to 67 years. The mean age was 4.33 years, and the median age was 1.35 years. Males predominated over females in all cleft types (60.4%), and the cleft lip was on the left side in 1,677 patients (46.4%). Most cases were unilateral (2,531; 70.0%) and complete (2,349; 64.9%), and involved a diagnosis of cleft lip and palate (1,981; 54.8%). Delayed primary labioplasty can affect daily functioning. Primary repair for patients with cleft lip and palate may be postponed due to limited awareness, socioeconomic factors, inadequate facilities, and varying adherence to treatment guidelines. Despite variations in the timing of primary cleft lip repair (not adhering to the recommended protocol), only 10% of these patients undergo reoperation. Healthcare providers should prioritize the importance of the ideal timing for primary repair in order to optimize physiological function without compromising the aesthetic results.

Manuscript_Socialization and Training on Tax Obligations for MSME to Support Tax Revenue in Indonesia

Taxes are an important source of state revenue. Every year, the contribution of taxes to state income increases, but it is not optimal. As observed, Micro, Small, and Medium Enterprise (MSME) is an important sector in the Indonesian economy, serving as one of the main contributors to state revenue. In order to improve this contribution, diverse strategies have been adopted by the government to augment tax paid by enterprise. One such strategy is the provision of various convenience facilities including online taxation services. However, it is equally crucial to also acknowledge that despite the provision of these convenience facilities, field observations showed a significant lack of interest and resistance from both the community and taxpayers concerning handling tax reports. Based on these conditions, lecturers at the Accounting Department at Universitas Padjadjaran conducted socialization and training webinars with the primary aim of providing substantial information concerning taxation for MSME in Indonesia. The results showed that after the conducted training, there was an increased understanding among MSME actors regarding tax regulations as well as an improved comprehension of tax calculations, payment compliance, and reporting.Keywords: Tax, MSME, Socialization and Training

Biocontrol potential of rhizospheric fungi against Rigidoporus microporus, the causal agent of white root rot disease in tea plants

White root rot disease (WRD), caused by Rigidoporus microporus, poses a serious threat to various tropical trees, resulting in substantial crop damage and even plant death. However, there is still a lack of information regarding WRD in tea plants. Due to soil-borne in nature, synthetic fungicide is not recommended to control WRD. Therefore, biocontrol using natural microorganisms found in the tea's rhizosphere has been explored as a potential solution. This study aimed to identify rhizospheric fungi of tea plants with antagonistic properties that can be used as a biocontrol agent against R. microporus. The study involved a field survey and laboratory works had been conducted in a tea plantation at Rancabali, West Java and the Phytopathology Laboratory, Faculty of Agriculture, Universitas Padjadjaran from March to October 2021. The disease incidence and severity were assessed in three blocks of land representing different topographic conditions of the plantation while antagonism tests were conducted using the completely randomized design and dual culture method. The results showed that the highest occurrence of WRD was in the central part of the hill with the disease incidence and severity of 70 and 50%, respectively. Thirteen fungal isolates were obtained from the rhizosphere of tea plants, all of which were found to inhibit the growth of R. microporus. These isolates belonged to the genera of Trichoderma, Gliocladium, Paecilomyces, Penicillium, Aureobasidium, Acremonium and Rhizopus. The considerable inhibition of R. microporus colony growth was performed by the isolates of Aureobasidium sp. (51.23%), Rhizopus sp. (50%), and Trichoderma sp. (43.96%). The results suggested that rhizospheric fungi of tea plants have the potential as biocontrol agents against R. microporus.