Hydrogen energy is one of the most promising sources of carbon neutrality from well to wheel. Among various applications of hydrogen energy, hydrogen internal combustion engines serve as the critical technical pathway. Nevertheless, several challenges persist, primarily related to abnormal combustion resulting from excessive flame propagation velocity. Of these challenges, backfire emerges as the most recurrent and pressing issue to be resolved in the port fuel-injected hydrogen internal combustion engine (PFI-H2ICE). In order to delve into the characteristics and mechanisms of backfire, this study concentrates on a critical internal parameter, namely the start of injection (SOI), and comprehensively assesses the backfire occurrences under varying lambda and engine speed conditions. Experimental findings reveal that SOI encompasses one of the pivotal factors influencing backfire. Backfire predominantly arises within two ranges of SOI conditions: the region before −430 degCA ATDC (Zone I) and the stretch between −360 degCA ATDC and − 280 degCA ATDC (Zone II). In the process of employing EGR to depress the effects of backfire on subsequent tests, it was incidentally found that the Universal Exhaust Gas Oxygen Sensor (UEGO) installed on the intake manifold for executing EGR rate calculations acted as a hotspot (with temperatures exceeding 100 °C). This not only intensified the backfire strength and the scope in Zone I but also caused the most severe backfire events to occur after −270 degCA ATDC (in Zone III). Despite this, the EGR strategy still demonstrated a significant backfire suppression effect, only exhibiting almost no influence on backfire caused by hotspots within the intake manifold. With an escalation in engine speed, the intensity of backfire diminishes, and the range of backfire occurrence decreases accordingly. By exercising control over SOI within the operating range from -430degCA to -360degCA ATDC (Zone A), the risk of backfire can be dramatically reduced, thereby achieving nearly backfire-free operation. Detailed analysis of cylinder pressure indicates that backfire may also give rise to other abnormal combustions, such as misfire, pre-ignition, and knock, in subsequent cycles. Moreover, this effect amplifies in tandem with an increase in mixture concentration. A certain degree of mutual promotion among diverse abnormal combustions is also observed. This study furnishes the most direct and practical foundation for internal and external strategies in the realm of backfire control.