Universal Adhesives Research Articles

Long-term Evaluation of Dentin Bonding Properties of the Photoinitiator System Contained in Universal Adhesives Used in Fiber-Post Luting Procedures.

This study evaluated the long-term push-out bond strength (PBS) and nanoleakage (NL) of universal adhesives with different photo-initiator systems in the root canals of teeth in which fiber posts were luted. One-hundred twenty endodontically treated human premolars were randomly divided into 12 groups based on the following factors: adhesives (Scotchbond Universal [SBU], Ambar Universal [AMB], and Ambar Universal APS [AMB-APS]); adhesive strategy (etch-and-rinse and self-etch), and time of testing (immediately vs after 2 years). The posts were cemented, sectioned into slices, tested for PBS at 0.5 mm/min, and examined for NL using scanning electron microscopy immediately thereafter or after 2 years of water storage. Data were evaluated using a four-way ANOVA (root thirds vs time vs universal adhesive vs adhesive strategies) and Tukey's test (α = 5%). For both evaluation times, AMB-APS demonstrated no significant difference in the PBS or NL when different radicular thirds were compared (p > 0.05). However, for SBU and AMB, the cervical third demonstrated higher PBS and lower NL than those of the apical third at both time points (p < 0.0001). AMB-APS exhibited higher PBS and lower NL in the apical third in comparison with SBU and AMB (p < 0.0001). SBU and AMB displayed a significant decrease in the PBS and increased NL after 2 years (p < 0.0001), whereas AMB-APS demonstrated no significant signs of degradation even after 2 years of water storage (p > 0.05). Independent of the root third evaluated, the universal adhesive containing APS photo-initiator system demonstrated bonding stability at the adhesive interface between the root canal and fiber posts, even after 2 years of water storage.

Influence of universal adhesives and silane coupling primer on bonding performance to CAD-CAM resin-based composites: A laboratory investigation.

Obtaining strong resin bonds to computer-aided design and computer-aided manufacturing (CAD-CAM) resin-based composites with dispersed fillers (CCRBCs) poses a challenge. Therefore, this study aimed to assess the effect of three universal adhesives and a two-component silane coupling agent on the shear bond strength to three (CCRBCs). Eight hundred and sixty-four specimens of Brilliant Crios, Lava Ultimate, and Tetric CAD were polished or grit blasted, bonded with Adhese Universal DC, One Coat 7 Universal (OC7), and Scotchbond Universal Plus Adhesive (SBU) with or without a silane primer. Shear bond strength was measured after 24 h and 10,000 thermocycles. Linear regressions were performed (α = 0.05). After thermocycling, bond strengths were similar for the universal adhesives on polished Brilliant Crios and Lava Ultimate (p ≥ 0.408). Grit blasted Tetric CAD showed no significant differences (p ≥ 0.096). The silane primer had minimal impact on grit blasted Tetric CAD (p ≥ 0.384). The silane primer increased the bond strength of OC7 to Brilliant Crios (p = 0.001) but decreased the adhesion of SBU to Brilliant Crios and Lava Ultimate (p ≤ 0.018). Bond strength of universal adhesives varied with CCRBC type. The two-component silane coupling agent showed mixed effects on adhesive performance. Selecting universal adhesives from the same CCRBC product line does not always guarantee superior bond strength. The efficacy of silane coupling agents differs based on the bonding substrate.

Bonding stability of universal adhesives to feldspathic ceramic with or without prior silane application.

This study aimed to assess the bonding stability of three universal adhesives to a CAD-CAM feldspathic ceramic with or without prior silane application. The universal adhesives tested were Ambar Universal (FGM), Single Bond Universal (3M), and Ybond Universal (Yller). Scotchbond Multipurpose Plus (3M) was used as a control. The silane used was RelyX Ceramic Primer (3M). Microtensile bond strength (μTBS) to Vitablocs Mark II was measured after 24 h or 6 months of water aging (n = 20). Failure modes were observed through scanning electron microscopy, and pH and degree of C = C conversion (DC) of the adhesives were measured (n = 3). Data were analyzed using One-, Two-, and Three-Way ANOVA. The pH values differed significantly among the adhesives (P<0.001), while the DC was similar (P = 0.141). The universal adhesives showed significant differences in μTBS, with higher immediate results when using silane and lower results after aging. Notably, a significant decrease in μTBS was observed after 6 months when silane was applied. In contrast, the control adhesive exhibited consistent results between the 24-hour and 6-month storage periods. The universal adhesives showed a decrease in μTBS ranging from 25.5% to 40.1% after prolonged storage. Adhesive failures were predominant in all groups. The presence or absence of silane in the adhesive composition did not affect the bonding performance. The application of a separate silane coupling agent improved the immediate bond strength of universal adhesives to CAD-CAM feldspathic ceramic. However, this bond strength significantly decreased after 6 months of water aging. The bond strength remained stable for universal adhesives when applied without silane after 6 months of aging. This article is protected by copyright. All rights reserved.

Does the application of dimethyl sulfoxide improve resin bonding to eroded dentine? Four-year in vitro evaluation.

To evaluate the effect of dimethyl sulfoxide (DMSO) on the microtensile bond strength (µTBS) and nanoleakage (NL) of universal adhesives on eroded dentine, immediately and after four years of water storage. Sixty-four sound human molars were distributed into 16 groups according to (1) Dentine surface (sound and eroded dentine); (2) dimethyl sulfoxide application (with or without); (3) Application mode (etch-and-rinse or self-etch) and (4) Storage time (immediate and four years). One mild universal adhesive was used (Scotchbond Universal). The restoration was then performed with a composite resin and the specimens were sectioned into resin-dentine bonded sticks. Resin-dentine bonded sticks were tested (immediately and after four years of water storage) for µTBS (0.5mm/min) or used to assess NL. Data on μTBS and NL were analyzed using four-way ANOVA and Tukey's test (α=0.05). Only the 3-way cross-product interaction 'substrate vs DMSO vs time' was statistically significant (p=0.007). Eroded dentine showed a lower mean of µTBS and a higher mean of NL values than sound dentine. However, when DMSO was applied, no significant decrease of μTBS or NL values was observed after four years of water storage, regardless of adhesive strategies, or dentine evaluated, when compared to immediate results. Water-based DMSO pre-treatments not only prevent degradation of MDP-containing simplified adhesives but also serve as a potential alternative to improve long-term bonding properties to eroded dentine. The versatility of using a single pre-treatment for both self-etch or etch-and-rinse bonding to eroded dentin may facilitate future clinical applications.

Bibliometric analysis of dental adhesives: research status and frontier development

Objective: A visual analysis of the literature in the field of dental adhesives is conducted in order to explore the current state of research, cutting-edge areas of interest, and future development trends in this domain.Methods: English literature related to dental adhesives published between 2000 and 2023 was searched in the Web of Science Core Collection database. The retrieved results were then imported into VOSviewer and CiteSpace software in plain text format. Various data, such as journal names, authors, institutions, countries, and keywords, were extracted for further bibliometric analysis.Results: A total of 19,403 publications were retrieved, featuring 42,365 authors, 7,359 institutions, 121 countries, and 1,523 journals. The annual publication and cumulative publication rates in this field are both on the rise. Among them, DENTAL MATERIALS is the journal with the highest publication rate, cumulative publication rate, and number of citations. Ozcan M is the author with the most publications and within the limitations of this study, is considered an influential author in the field (with the highest intermediary centrality score) and Meerbeek B is the author with the highest number of citations. UNIV SAO PAULO is the institution with the highest publication rate. The United States is the country with the highest publication rate and has the most collaborative partnerships with other countries. Collaboration between different authors, institutions, and countries in this field is indeed close, which has greatly contributed to the rapid development of dental adhesives. Current research focuses on various aspects such as the types of dental adhesives, adhesive strength, dental diseases, and clinical trials. Future research directions mainly concentrate on aspects such as nanoparticles, 3D printing, universal adhesives, antibacterial properties of adhesives, and adhesive strength.Conclusion: Within the defined scope of this study, we have conducted a quantitative and objective analysis of the current research status and emerging trends in dental adhesives. This analysis establishes a knowledge foundation and introduces novel perspectives for future in-depth investigations in this field.

Evaluation of Shear Bond Strength of Resin Cement on the Surface of a Lithium Disilicate Glass-Ceramic Restorative Material after Various Surface Treatments

With bonded restorations gaining rapid popularity in clinical dentistry, manufacturers have introduced a variety of bonding protocols and materials. These materials, including surface modifiers and cleaning agents, are designed to decontaminate surfaces and enhance bonding effectiveness. In this study, six different combinations of mechanical and chemical modifications were tested on a lithium disilicate surface to determine the combination that offers optimal resistance to shear stresses. The tested surface modifications included 9% hydrofluoric acid, sandblasting with 29 μm aluminum oxide (Al2O3) particles, Ivoclean (a recently introduced decontamination agent), Monobond Etch &amp; Prime (a one-stage etching and priming agent for ceramic surfaces), Monobond Plus (a silane agent), and the bonding agent Adhese Universal. Six different sequence combinations were tested and compared to the negative control group. The highest bond strength was achieved using all materials and cleansing methods in a logical order, while the bond strength was lowest in the absence of surface modification (control group). The results indicate a significantly positive influence on bond strength of silane coupling agents present in surface modifiers, including pure forms like Monobond Plus. Potential negative effects of cleansing agents or methods on bond strength were not observed. Multiple and separate stages in the treatment of the lithium disilicate surface positively impact bond strength. Cleansing agents may prove beneficial in clinical conditions, and they do not interfere with bonding.

To etch or not to etch, Part III: On the hydrophobic-rich content and fatigue strength of universal adhesives after long-term ageing

ObjectivesTo examine whether dentin-etching extension and/or the hydrophobic-rich content of hybrid layers would affect fatigue strengths of a mild universal adhesive after long-term aging. MethodsTwin-bonded resin-dentin interfaces were produced by etching sound midcoronal dentin beams with 32 % ortho-phosphoric acid for 15 s (OPA15s), 3 s (OPA3s) or 10 % meta-phosphoric acid for 15 s (MPA15s). Samples were bonded with a mild universal adhesive with or without additional coating using a solvent-free bisGMA-based bonding resin. Self-etch application served as control. Composite buildups were made with a nanofilled composite. Bar-shaped twin-bonded interfaces (0.9 × 0.9 × 12 mm) were aged for two years in artificial saliva at 37 ℃ and tested under 4-point flexure at quasi-static (n = 16) and cyclic loads (n = 35) until failure. The stress-life fatigue behavior was evaluated using the staircase method at 4 Hz. Crack initiation and fracture patterns were evaluated by SEM. Cyclic-loaded data was analyzed by Kruskal-Wallis on Ranks (α = 0.05). ResultsSignificantly higher fatigue life distributions and higher endurance limits were observed for less aggressive etch-and-rinse protocols (OPA3s and MPA15s) after long-term ageing. Hydrophobic-rich coating produced 20–32 % higher endurance limits, prevented micrometer-sized porosities at bonded interfaces, reduced etching-associated variability and lowered crack formation.SignificanceLong-term hydrolysis produces detrimental effects on the fatigue strength of resin-dentin interfaces. The bond-promotion effect of less aggressive etch-and-rinse protocols and the creation of hybrid layers with higher hydrophobic-rich content are critical to extend the durability of mild universal adhesives. Therefore, current oversimplification trends in adhesive dentistry may limit resin-dentin bonding performance.

The Effect Of Er,Cr:YSGG Laser Conditioning on Dentin Bond Strength and Nanoleakage of Universal Adhesive Systems: An In Vitro Study.

To investigate the effect of erbium, chromium: yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser conditioning on dentin bond strength and nanoleakage of different universal and self-etch adhesives. A total of 84 intact human third molar teeth were cut at the dentin level, and half of them were laser conditioned. The specimens were divided into three groups; two different universal resins and one self-etch adhesive resin were applied and composite resin restorations were created. For the microtensile bond strength (µTBS) test, 20 microspecimens were prepared from the laser and control group of each adhesive (n = 20) and tested with a universal device. For nanoleakage observation, 10 specimens were prepared from each group (n = 10) and stored in silver nitrate solution, and then the amount of nanoleakage was analyzed by field emission scanning electron microscopy (FESEM). Data were analyzed with two-way ANOVA, Tukey's honestly significant difference, and chi-square tests. The mean dentin bond strength of all adhesives in the laser groups was found to be statistically significantly lower than those of the control groups (P < .05). No difference was found between the mean bond strength of the adhesives in the laser and control groups (P > .05). Higher nanoleakage was observed in all adhesives in the laser groups compared to the control groups (P < .05). Irradiation of the dentin surface with Er,Cr:YSGG could adversely affect the µTBS and nanoleakage, likely by affecting the structure of the hybrid layer.

Comparative Evaluation of Application of Universal Bonding on the Microtensile Bond Strength of Light Cure and Dual Cure Composites Bonded to Dentin.

Objectives: There is limited knowledge on the efficacy of universal adhesives when used in different etching modes. The aim of this study was to evaluate the microtensile bond strength (µTBS) to dentin using universal adhesives with light-cure and dual-cure composites. Materials and Methods: In this in vitro experimental study, the occlusal third of 60 caries-free human molars were removed and the exposed surfaces were treated and allocated to 10 groups (N=6). Two different bonding agents (Single Bond Universal, G-Premio) were used to bond 2 different composite resins (Z250 light-cure and CoreFlo DC dual-cure) to dentin, using etch-and-rinse (E&R) and self-etch (SE) modes. Single Bond 2 was applied in E&R mode on wet dentin to serve as control. The specimens were sectioned into 1×1 mm2 sticks with a precision saw. A microtensile testing machine was used to measure the µTBS of the specimens with a crosshead speed of 0.5 mm/min. Data were analyzed using three-way ANOVA and Bonferroni post-hoc test (α=0.05). Results: The Single Bond Universal group in SE mode with light-cure composite yielded the highest (39.24MPa), and the G-Premio SE group with dual-cure composite produced the lowest (13.65MPa) mean µTBS (P<0.05). The dominant failure mode was adhesive, in all groups. Conclusion: The bonding agent had a significant effect on µTBS of composite to dentin, but different composites and different etching modes were not significantly different in this respect.

Dentin bond strength and antimicrobial activities of universal adhesives containing silver nanoparticles synthesized with Rosa canina extract.

The purpose in the study was to evaluate the effect of biogenic silver nanoparticles (Ag NPs) synthesized by the green synthesis method on dentin bond strength in three different universal adhesives and investigate their antibiofilm activity against Streptococcus mutans (S. mutans). Three different universal adhesives (single bond universal, all-bond universal, and clearfil universal) were used in this study. Ag NPs were synthesized using rose hip (Rosa canina) extract as a reducing and stabilizing agent and they were characterized with STEM, UV-vis spectrophotometer, DLS, and zeta potential. Ag NPs were added to the adhesive resins at a rate of 0.05% (w/w), and their homogeneous distribution in the adhesive was determined using EDX spectrometry. Samples in all groups were tested at baseline-after 5000 and 10,000 thermal cycles. Adhesive composite discs were used for the live/dead analysis of S. mutans, MTT metabolic activity test, lactic acid production, and determination of colony-forming unit (CFU) values (n = 3). Ninety extracted caries-free human third molars were used to determine microtensile bond strength (μTBS) (n = 10). After the universal adhesive was applied to the dentin surface, composite resin (Z550 XT, 3M ESPE, USA) was placed and sections were taken to form a composite-dentin stick of 1mm × 1mm wideness and 8-mm length. The sticks were broken at a rate of 1mm/min under uniaxial tension in a universal testing machine, and the failure modes were determined by SEM. One-way analysis of variance (ANOVA) for antibacterial tests and two-way analysis of variance for μTBS tests were performed (p < 0.05). All universal adhesive groups containing Ag NPs showed higher antibacterial activity than control groups without Ag NPs (p < 0.05). There was a statistically significant difference in the live/dead assay analysis, MTT metabolic activity test, lactic acid production, and CFU values in the thermal cycled Ag NPs groups (p < 0.05). Antibacterial activity decreased in groups containing Ag NPs subjected to 10,000 thermal cycles. The highest lactic acid production 11.06 (± 0.629) and CFUs 7.61 (± 0.304), live bacteria 31.13 (± 0.466), and S. mutans MTT metabolic activity 0.29 (± 0.376) at AU (All-Bond Universal-Ag NPs) 10,000 thermal cycles group. There was no difference in μTBS values between the initial and 5000 thermal cycle groups, there was a difference between the 10,000 thermal cycle groups. The CU (Clearfil Universal-Ag NPs) group subjected to 10,000 thermal cycles showed lower μTBS 11.1 (± 3.2). In conclusion, universal adhesives containing biogenic Ag NPs showed higher antibacterial activity than the control groups and did not reduce the μTBS. Antibacterial universal adhesives can contribute to restoration success in clinical applications by reducing residual bacteria and preventing secondary caries formation.

Are Universal Adhesives Effective for Bonding to Zirconia in the Long Term?

The bond capacity of universal adhesives should be comparable to a specific primer for zirconia. Thus, this study evaluated the bond strength to zirconia of four universal adhesives and a zirconia primer over long-term storage. The surfaces of 75 samples of zirconia were sandblasted with 50 µm aluminum oxide particles and then divided into groups (n = 15): G1 - Single Bond Universal (SBU); G2 - All Bond Universal; G3 - Peak Universal Bond; G4 - Ambar Universal (AU), and G5 - Z-Prime Plus (ZP). A cone of resin composite was constructed on the applied materials. The samples were submitted to a tensile bond strength test after 24 h using a universal testing machine. Then, the remaining materials were removed from the sample surfaces, and the surfaces were polished and sandblasted again as previously described to obtain the same groups. These new samples were stored in distilled water at 37°C for 12 months and then submitted to a tensile bond strength test. The data were analyzed by two-way analysis of variance and Tukey's test (α =0.05). The material factor (P = 0.001) and the storage factor (P = 0.001) were significant, and the interaction was not significant (P = 0.117). According to Tukey's test, bond strength mean values (in MPa) followed by distinct letters were significantly different. After 24 h, G5 = 21.12 A, G1 = 20.55 A, G4 = 19.19 AB, G2 = 14.22 B, and G3 = 8.44 C. After 12 months, G1 = 7.37 A, G5 = 5.61 AB, G4 = 4.97 B, G2 = 3.32 C, and G3 = 1.93 D. After 12 months of storage, all groups' bond strength significantly decreased. SBU and AU had bond strengths comparable to ZP after 24 h. No material resisted water degradation.

Effect of Er,Cr:YSGG and Nd:YAG Lasers on Microleakage of Composite Resin Restorations Using Universal Adhesives

Background: Clinical long-term success of bonded restorations is depended to reduce marginal microleakage and the laser application with combination of new bonding systems may play positive role. The aim of this study was comparative evaluation of Er,Cr:YSGG and Nd:YAG lasers effect on microleakage of class V composite resin restorations using several universal adhesives. Methods: In this in vitro study, standard class V cavities were prepared on both lingual and buccal surfaces of 72 intact premolar teeth. For evaluation of microleakage, the cavities were divided into 9 groups according to conditioning method (n=15): G1: G-Premio Bond [GP]; G2: Scotchbond Universal Adhesive [SU]; G3: All-Bond Universal [AB]; G4: GP+Nd:YAG Laser; G5: SU+Nd:YAG Laser; G6: AB+Nd:YAG Laser; G7: GP+Er,Cr:YSGG Laser; G8: SU+Er,Cr:YSGG Laser; and G9: AB+Er,Cr:YSGG Laser. The cavities were filled with composite resin and then subjected to thermocycling, placed in methylene blue solutions, embedded in resin blocks and vertically cut in the bucco­lingual direction. The microleakage in occlusal and gingival margins was defined as linear penetration of methylene blue and determined with a stereomicroscope to assign microleakage scores using a four­point scale. Data were analyzed by using SPSS software and Kruskal-wallis and Mann-Whitney U tests. Results: Microleakage scores had statistically significant different before and after laser irradiation in dependent to the adhesive used (P&lt;0.05) but there were no significant different between Nd:YAG and ER,Cr:YSGG laser, also between different universal adhesives (GP, SU, AB). The results showed that total microleakage scores of occlusal margins had significant statistical different in comparison with gingival margins (P&lt;0.001). Conclusion: The results indicate that Nd:YAG and Er,Cr:YSGG lasers irradiation, after using the investigated universal adhesives, had a positive effect on reducing the microleakage of composite Class V restorations. The microleakage rate was shown to be independent of adhesives and lasers type used in this study.

Universal Adhesives in Clinical Dentistry

Universal Adhesives in Clinical Dentistry

Does dimethyl sulfoxide promote the bonding performance of different universal adhesives to dentin in etch-and-rinse approach?

This study examined the influence of dimethyl sulfoxide (DMSO) on the microtensile bond strength (μTBS) of universal adhesives to dry or wet dentin in the etch-and-rinse approach. Human third molars were cut to reveal the flat mid-coronal dentin surface. The specimens were randomly divided into 16 groups (n = 8) based on dentin condition (dry or wet), dentin pretreatment (with or without DMSO), and adhesive agents (Scotchbond Universal (SBU), Ambar Universal Bond (AUB), G-Premio Bond (GPB), and Clearfil Universal Bond (CUB). The etched-dentin surfaces were pretreated with a 50% DMSO solution before adhesive application. After 24 h or thermocycling, the resin-dentin beams were tested using the microtensile device. μTBS data were analyzed using a four-way ANOVA (α = 0.05). Scanning electron microscopy (SEM) was used to detect nanoleakage at adhesive interfaces. DMSO increased μTBS to wet and dry dentin of CUB, SBU, and AUB but not GPB. CUB, SBU, and AUB bonded better to wet dentin than dry dentin, but GPB bonded equally well to both. DMSO's nanoleakage effect dependent on dentin moisture and the adhesive agent used.

The role of camphorquinone in the cytotoxicity of universal dental adhesives

This work aimed to examine the effect of unreacted substances of four universal adhesives on cell viability, taking into account different sample preparation protocols. The protocols for sample preparation were as follows: P1: Disc-shaped specimens made of the adhesive material itself; P2: filter paper disc impregnated with the adhesive system; P3: bovine dentin disc impregnated with the adhesive system. The UHPLC/Q-TOF-MS spectroscopy was utilized to detect the type and quantity of unreacted substances released. The cell viability of the universal adhesives was evaluated considering the sample preparation protocol, as well as the different concentrations of each compound detected by the UHPLC/Q-TOF-MS spectroscopy in their isolated form. The WST-1 assay was employed to assess cell viability. From the extraction media, HEMA, BisGMA, CQ, EDAB, TPO, and UDMA were identified. When evaluated in P1, significantly higher amounts of CQ were detected in all of the universal adhesives (p<0.001). The sample preparation protocol was found to significantly influence cell viability (p<0.001). Notably, all universal adhesives exhibited a cytotoxic effect only when using the P1 protocol. Among the analyzed substances, CQ was identified as the most cytotoxic component (p<0.05). It was observed that disc-shaped specimens made with the material itself resulted in an overestimation of the cell viability outcomes for the universal adhesives.

Influence of surface treatments and adhesive protocols on repair bond strength of glass-matrix and resin-matrix CAD/CAM ceramics.

To specify the effects of self-etching ceramic primer, Monobond Etch and Prime (MEP), and universal adhesive (UA) on repair bond strength between CAD/CAM blocks and resin composite. Vita Mark II (VM), IPS e.max CAD (EMAX), Shofu Block HC (SHC), and Tetric CAD (TET) blocks were sliced and thermocycled. They were divided into four groups according to surface treatments (n = 24): control, sandblasting (AL), hydrofluoric acid etching (HF), and MEP application. SEM analysis assessed surface topography. Subdivided, specimens followed distinct adhesive protocols (n = 12): control (silane + adhesive [SA] or adhesive [A] only for MEP group) and UA. Microshear bond strength (μSBS) was measured following resin composite repair. Data were analyzed using two-way ANOVA and Tukey tests (p < 0.05). The μSBS of CAD/CAM blocks was significantly influenced by surface treatment type and adhesive protocol. The highest μSBS values for each block, considering surface treatment + adhesive protocol, were VM, HF + SA, or HF + UA; EMAX, MEP + A; and SHC and TET, AL + SA, or AL + UA. Except for EMAX, it was not the adhesive protocol that made the significant difference in bond strength for each CAD/CAM block, but the surface treatment. MEP + UA application on glass ceramics lowered μSBS values significantly, so it is not recommended in clinical conditions. Repair is an essential therapeutic option, particularly in esthetic restorations, to swiftly repair the impaired esthetics caused by fracture. Repair protocol is dependent on the restorative material, and to have a reliable repair bond strength, the following surface treatment and adhesive protocol combinations are recommended for each CAD/CAM block: VM, HF + SA, or HF + UA; EMAX, MEP + A; and SHC and TET, AL + SA, or AL + UA.

Evaluation of different decontamination procedures on bond strength to sound and caries affected dentin using “no-wait” universal adhesive

BackgroundCurrent study aimed to evaluate the effect of different decontamination procedures on micro-shear bond strength (μSBS) of sound (SoD) and caries-affected dentin (CAD) of two universal adhesives after blood-saliva contamination.MethodsOne hundred and eighty bovine anterior teeth were prepared and allocated into the respective groups according to tested dentin substrates [SoD, CAD], universal adhesives [Clearfil Bond Universal Quick (UBQ), All-Bond-Universal (ABU)], adhesive contamination stage [none, contamination before and after adhesives light-curing], and according to decontamination procedures [no decontamination, water rinsing, adhesive rebond, Ethylenediaminetetraacetic acid (ETDA) and chlorhexidine (CHX) application]. Universal adhesives were applied according to manufacturer instructions in self-etch (SE) bonding mode. Four composite microrods were built for each tooth. Specimens were kept in distilled water for 24 hours at 37°C before testing μSBS. Four-way ANOVA and Tukey HSD tests were used for data analysis.ResultsA statistically significant difference between contamination stages of both universal adhesives at different decontamination procedures for SoD and CAD. Highest μSBS was recorded for UBQ control group at SoD, while the least was recorded for light-cured ABU upon water rinsing decontamination procedure of CAD.ConclusionsProper cavity isolation is mandatory to avoid possible contamination which can dramatically affect μSBS. CHX is a potent cavity decontaminant that can restore different dentin substrates bond strength. EDTA presents a promising substitute. UBQ adhesive showed better bonding performance than ABU to both dentin substrates. Application of regular cavity decontamination approaches is highly advised in daily practice to avoid possible detrimental effect of accidental cavity contamination.

Effect of the Adhesive Strategy on Clinical Performance and Marginal Integrity of a Universal Adhesive in Non-Carious Cervical Lesions in a Randomized 36-Month Study.

The effectiveness of a universal adhesive applied in three application modes for the preparation of Class V composite restorations was evaluated both clinically and by quantitative marginal analysis (QMA) over 36 months. In 50 patients, three (n = 21) or four (n = 29) non-carious cervical lesions (NCCL) were restored with Venus® Diamond Flow (Kulzer GmbH, Hanau, Germany). The adhesive iBond® Universal (iBU, Kulzer, Germany) was used in self-etch (SE), etch-and-rinse (ER), or selective-enamel-etch mode (SEE). The etch-and-rinse adhesive OptiBondTM FL served as a control (OFL, Kerr GmbH, Herzogenrath, Germany). The restorations were clinically assessed (FDI criteria) at 14 days (BL), 6, 12, 24, and 36 months. Additionally, QMA was conducted on all restorations of 11 randomly selected patients. FDI criteria and marginal gap and perfect margin were compared between and within groups and recalls using McNemar, Wilcoxon, or Mann-Whitney U-tests (α = 0.05). Starting with 12 months, cumulative failure rates were lower in iBU-SE (0.0%, p = 0.016) and iBU-ER groups (2.1%, p = 0.07) compared to OFL (16.7%). At two years, iBU-SEE also showed fewer failures (0.0% SEE vs. 34.6% OFL, p = 0.016), as did iBU-SE compared to iBU-ER after 36 months (2.2 and 19.6%, p = 0.039). From BL, the iBU-SEE group always had the fewest marginal gaps and the highest percentage of perfect margins. From BL, iBU-SEE (0%, p = 0.008) and iBU-ER (0.2%, p = 0.027) showed significantly fewer marginal gaps compared to OFL (2.5%) and more perfect margins were found with iBU-SEE starting at 6 months (p = 0.054). The SEE and ER modes ensured the most excellent marginal quality, with differences from the control appearing earlier with QMA than clinically. In restoring NCCls, iBU showed superior clinical performance over OFL, especially in modes SE and SEE.

Clinical performance of non-carious cervical lesions restored with using self-etch mode of adhesives according to the FDI criteria.

The purpose of this study was to evaluate the 18-month retrospective study of self-etch adhesive (Prime&Bond One Select) and self-etch mode of universal adhesives (Single Bond Universal, Gluma Bond Universal) applied to non-carious cervical lesions according to the World Dental Federation criteria. Thirty patients without any systemic disease, good oral hygiene, having at least 20 teeth and six non-carious cervical lesions treated using a self-etch mode of adhesives operated between January and March 2017. Accordingly, 335 restorations were evaluated according to the World Dental Federation criteria at 3, 6, 12 and 18 months. Pillai's Trace test was used to determine the interaction of criteria with time and adhesive systems. IBM SPSS Statistics 21.0 program was used in the analysis. Value of P < 0.05 was accepted as a criterion for statistical significance. At the end of 18 months, participation was 100%. There was a statistically significant difference P < 0.05 between Prime&Bond One Select and Single Bond Universal, Prime&Bond One Select and Gluma Bond Universal in the 18-month period. In terms of marginal staining, fracture of material and retention, recurrence of caries, erosion and abfraction, tooth integrity criteria at the end of the 18 months, Prime&Bond One Select performed clinically very good, Single Bond Universal and Gluma Bond Universal performed clinically good according to World Dental Federation criteria. Marginal adaptation criteria, Prime&Bond One Select, Single Bond Universal and Gluma Bond Universal performed clinically good. Retention loss rate was found to be the lowest in Prime&Bond One Select and the highest in Gluma Bond Universal. Accordingly, 18-month evaluation of all adhesives used in the study showed clinically acceptable results in all of the World Dental Federation criteria.

Effect of the Absence of HEMA on the Bonding Properties of Universal Adhesive Systems Containing 10-MDP: An In Vitro Study.

To evaluate the absence of 2-hydroxyethyl methacrylate (HEMA) on the adhesive properties with enamel and dentin of universal adhesive systems containing 10-methacryloyloxydecyl dihydrogen phosphate (MDP). One hundred and twelve caries-free third molars were used to test adhesion to dentin (n=64) and enamel (n=48). For each substrate, teeth were divided into eight experimental groups: four different adhesives each using two adhesive strategies. The adhesives used were: (1) Scotchbond Universal (SBU, 3M Oral Care, St Paul, MN, USA) as a HEMA-containing universal adhesive; (2) Gluma Bond Universal (GBU, Kulzer, Hanau, Germany); (3) Solare Universal Bond (SUB, GC, Tokyo, Japan); and (4) Zipbond Universal (ZIP, SDI, Victoria, Australia) as HEMA-free universal adhesives. The adhesive strategies used were etch-and-rinse (ER) and self-etch (SE). For dentin tests, the occlusal third of the crown of all teeth was removed and an adhesive protocol was applied. After completing the restoration, specimens were sectioned into bonded sticks (0.8 mm 2) and tested for microtensile bond strength (μTBS), in situ degree of conversion (DC), and nanoleakage (NL) by scanning electron microscopy. For enamel tests, teeth were sectioned into four parts (buccal, lingual, and proximal), and an adhesive protocol was applied. After completing the restoration, the specimens were tested for their microshear bond strength (μSBS). For in situ degree of conversion (DC) and nanoleakage (NL) evaluation of enamel, the specimens were sectioned in slices to be evaluated. The data for each substrate were subjected to two-way ANOVA and Tukey's test (α=0.05) for each property evaluated. The SBU and ZIP adhesives showed the highest μSBS, and DC (dentin and enamel) and lower NL (dentin) values compared to GBU and SUB (p=0.001). However, SBU showed better results in terms of μTBS and μSBS (SE strategy), and DC (dentin and enamel) than ZIP. Strategy ER presented higher values of μTBS and μSBS when compared to strategy SE (p=0.001), except for SBU. The effect of the absence of HEMA in commercial universal adhesive systems on enamel and dentin adhesive properties appears to be material-dependent.