Unique Variation Research Articles

Unearthing Genetic Treasures: Exploring Lost Autochthonous Vitis vinifera Varieties in Lebanon

Background/Objectives: Lebanon, one of the oldest centers of grapevine (Vitis vinifera L.) cultivation, is home to a rich diversity of local grape varieties. This biodiversity is linked to the country’s unique topography and millennia of cultural history. However, the wine industry primarily utilizes international varieties, putting many local varieties at risk of extinction. Methods: In this study, we analyzed 202 samples from old vineyards, home gardens, and private collections using 21 microsatellite markers to assess their identity and genetic diversity. Results: A total of 67 different genotypes were identified, with 34 not matching any existing profiles in the consulted databases, based on comparisons with the European Vitis Database, the Vitis International Variety Catalogue (VIVC), and the databases established in two previous studies conducted in Armenia and Lebanon. Cluster analyses revealed Lebanon’s rich diversity of local grape varieties, highlighting cases of synonymy, homonymy, and misnaming. All loci were polymorphic, with 228 alleles and an average of 11.4 alleles being detected. The highest number of alleles was observed at the VVIV67 locus (19 alleles), while the lowest was found at the VVIQ52 and VVIN73 loci (5 alleles). The observed heterozygosity was 0.732, slightly below the expected value of 0.757, with gene diversity varying among the markers. Conclusions: Of the 67 genetic profiles identified, 34 are absent from national and international databases, underscoring Lebanon as a hotspot for grapevine genetic diversity. This unique genetic variation, which includes several synonyms due to geographic isolation, could provide valuable opportunities for producing distinctive wines and emphasizes the need for further research and documentation.

Extra-cavity modulating a dark bisoliton

Abstract Although the concept of modulating optical solitons out of fiber laser cavities has already been proposed, to more flexibly change optical solitons’ time or frequency domain properties, compared with traditional intra-cavity modulation. The extra-cavity modulation of dark bisolitons has seldom been touched upon. In this manuscript, we theoretically conduct the research about modulating a dark bisoliton at 1 μm wavelength regime, in an extra-cavity modulation system. The modulation system is composed of different modulation elements. In the simulation, we consider the variations of different parameters. And modulated dark bisoliton’s orthogonal polarization components will show unique variation properties as these soliton parameters vary. For example, as time delay in orthogonal directions varies, a new small pulse peak appears at the position of continuous wave background in vertical polarization direction at output port, when time delay reaches to a specific value. These simulation results further explore the extra-cavity modulation of dark pulses in nonlinear optical fiber modulation systems, which are useful to understand the dark bisolitons’ dynamics in extra-cavity modulation systems. And these results have potential applications in the field of high-speed and long-distance optical fiber communication.

Unveiling the versatility of the thioredoxin framework: Insights from the structural examination of Francisella tularensis DsbA1

In bacteria the formation of disulphide bonds is facilitated by a family of enzymes known as the disulphide bond forming (Dsb) proteins, which, despite low sequence homology, belong to the thioredoxin (TRX) superfamily. Among these enzymes is the disulphide bond-forming protein A (DsbA); a periplasmic thiol oxidase responsible for catalysing the oxidative folding of numerous cell envelope and secreted proteins. Pathogenic bacteria often contain diverse Dsb proteins with distinct functionalities commonly associated with pathogenesis. Here we investigate FtDsbA1, a DsbA homologue from the Gram-negative bacterium Francisella tularensis. Our study shows that FtDsbA1 shares a conserved TRX-like fold bridged by an alpha helical bundle showcased by all DsbA-like proteins. However, FtDsbA1 displays a highly unique variation on this structure, containing an extended and flexible N-terminus and secondary structural elements inserted within the core of the TRX fold itself, which together twist the overall DsbA-like architecture. Additionally, FtDsbA1 exhibits variations to the well conserved active site with an unusual dipeptide in the catalytic CXXC redox centre (CGKC), and a trans configuration for the conserved cis-proline loop, known for governing DsbA-substrate interactions. FtDsbA1’s redox properties are comparable to other DsbA enzymes, however, consistent with its atypical structure, functional analysis reveals FtDsbA1 has a high degree of substrate specificity suggesting a specialised role within F. tularensis’ oxidative folding pathway. Overall, this work underscores the remarkable malleability of the TRX catalytic core, a ubiquitous and ancestral protein fold. This not only contributes to broadening the structural and functional diversity seen within proteins utilising this core fold but will also enhance the accuracy of AI-driven protein structural prediction tools.

Networks extracted from nonlinear fMRI connectivity exhibit unique spatial variation and enhanced sensitivity to differences between individuals with schizophrenia and controls

Schizophrenia is a chronic brain disorder associated with widespread alterations in functional brain connectivity. Although data-driven approaches such as independent component analysis are often used to study how schizophrenia impacts linearly connected networks, alterations within the underlying nonlinear functional connectivity structure remain largely unknown. Here we report the analysis of networks from explicitly nonlinear functional magnetic resonance imaging connectivity in a case–control dataset. We found systematic spatial variation, with higher nonlinear weight within core regions, suggesting that linear analyses underestimate functional connectivity within network centers. We also found that a unique nonlinear network incorporating default-mode, cingulo-opercular and central executive regions exhibits hypoconnectivity in schizophrenia, indicating that typically hidden connectivity patterns may reflect inefficient network integration in psychosis. Moreover, nonlinear networks including those previously implicated in auditory, linguistic and self-referential cognition exhibit heightened statistical sensitivity to schizophrenia diagnosis, collectively underscoring the potential of our methodology to resolve complex brain phenomena and transform clinical connectivity analysis.

Diversities of chromite mineralization induced by chemo–thermal evolution of the mantle during subduction initiation

Ophiolites, mostly formed via subduction initiation at proto-forearcs, exhibit a unique variation of mantle-derived magmatism from MORB-like to low-Ti tholeiitic and bainitic-like affinities. Such variation was suggested to form chromite deposits spanning high-Al to high-Cr types. Nevertheless, the origin of diverse magmatism during subduction initiation and their linkages to different chromite deposits has long been enigmatic. Here we show elemental and Os isotopic compositions of different chromitites from the Zambales ophiolite, Philippines. Combined with data from ophiolites worldwide, high-Al and high-Cr chromitites are revealed to result from low-Ti tholeiitic and boninitic-like magmatism, respectively. Proto-forearc mantle had few chromitites generated during MORB-like magmatism, but afterwards, it was modified first by slab fluids and later by continuous asthenospheric upwelling in the context of slab densification and rollback. The latter modification elevated the geothermal gradient and replenished fertile components in the proto-forearc mantle progressively, inducing increasingly higher degrees of mantle melting and Cr-richer magmatism and chromitites.

Efficient feature selection for histopathological image classification with improved multi-objective WOA

The difficulty of selecting features efficiently in histopathology image analysis remains unresolved. Furthermore, the majority of current approaches have approached feature selection as a single objective issue. This research presents an enhanced multi-objective whale optimisation algorithm-based feature selection technique as a solution. To mine optimal feature sets, the suggested technique makes use of a unique variation known as the enhanced multi-objective whale optimisation algorithm. To verify the optimisation capability, the suggested variation has been evaluated on 10 common multi-objective CEC2009 benchmark functions. Furthermore, by comparing five classifiers in terms of accuracy, mean number of selected features, and calculation time, the effectiveness of the suggested strategy is verified against three other feature-selection techniques already in use. The experimental findings show that, when compared to the other approaches under consideration, the suggested method performed better on the assessed parameters.

Population genetics of the freshwater red alga Batrachospermum gelatinosum (Rhodophyta) II: Phylogeographic analyses reveal spatial genetic structure among and within five major drainage basins in eastern North America.

The freshwater red alga Batrachospermum gelatinosum has a well-documented distribution spanning historically glaciated and unglaciated eastern North America. This alga has no known desiccation-resistant propagule; thus, long-distance dispersal events are likely rare. We predicted strong genetic structure among drainage basins and admixture among sites within basins. We predicted greater genetic diversity at lower latitude sites because they likely serve as refugia and the origin of northward, post-Pleistocene range expansion. We used 10 microsatellite loci to investigate genetic diversity from 311 gametophytes from 18 sites in five major drainage basins: South Atlantic Gulf, Mid-Atlantic, Ohio River, Great Lakes, and Northeast. Our data showed strong genetic partitioning among drainage basins and among sites within basins, yet no isolation by distance was detected. Genetic diversity varied widely among sites and was not strictly related to latitude as predicted. The results from B. gelatinosum provide strong support that each stream site contributes to the unique genetic variation within the species, potentially due to limited dispersal and the prevailing reproductive mode of intragametophytic selfing. Simulations of migration suggested post-Pleistocene dispersal from the Mid-Atlantic. Batrachospermum gelatinosum potentially persisted in refugia that were just south of the ice margins rather than in the southernmost part of its range. Research of other taxa with similar ranges could determine whether these results are generally applicable for freshwater red algae. Nevertheless, these results from B. gelatinosum add to the growing literature focused on the patterns and genetic consequences of post-Pleistocene range expansion by eastern North American biota.

Ectopic Dental Canal: A Case Report Highlighting a Unique Bifid Mandibular Canal Variant

Bifid mandibular canals are common anatomical findings with variations based on direction and location of branching, which carry significant clinical implications for endodontic and surgical dental procedures. This case report describes a previously unreported ectopic dental canal that branches off the superior border of the mandibular canal, enters the apex of a mandibular second molar, traverses through the root, anastomoses with the root canal system, and subsequently exits through the lingual aspect of the root. The anastomosis of this ectopic dental canal with the mesial lingual canal led to significant bleeding during rotary instrumentation. This unique anatomical variation demonstrates the importance of a thorough cone-beam computed tomography analysis to identify critical structures prior to undertaking dental procedures involving the root apices of mandibular posterior teeth and adjacent areas.

Unique Seasonal Variation in Rainfall Diurnal Features on the Yunnan–Guizhou Plateau

Based on hourly precipitation observations, the diurnal variation in precipitation and its seasonal evolution over the Yunnan–Guizhou Plateau (YGP) were analyzed. The results indicate that the seasonal variation in hourly rainfall in the western part of the YGP is unique. The rainfall reaches its hourly maximum during the late afternoon in spring (March–April) and during nighttime in summer (July–August), which contrasts with the pattern in most of eastern China. By further classifying the rainfall into short-duration (1–3 h) and long-duration (more than 6 h) events, the unique seasonal variations in the western YGP are found to mainly be comprised of short-duration rainfall. The long-duration rainfall shares similar diurnal peaks year-round for both the western and eastern parts of the YGP. The short-duration rainfall in the western part of the YGP shows a year-round afternoon peak, which is different from that of the eastern YGP, which peaks from midnight to early morning in spring and in the late afternoon in summer. The surface maximum daily temperature and low-level instabilities reach their annual maximum in spring over the western YGP and are also higher than in other parts of the YGP, together providing favorable conditions for convection to be triggered in spring afternoons over the western YGP.

How important is Forest Age in explaining the species composition of Near-natural Spruce Forests?

Multiple ecological drivers, along with forest age, determine the species composition of boreal forest ecosystems. However, the role of age in successional changes in forests cannot be understood without taking site conditions, the disturbance regime and forest structure into account. In this study, we ask two research questions: 1. What is the relationship between forest age and overall species composition in older near-natural spruce forests, i.e. forests of age beyond harvest maturity? 2. Do species associated with different forest habitats respond similarly to variation in forest age? Data were collected in 257 Norway spruce dominated 0.25 ha plots from three study areas in Southeastern and Central Norway. Species inventories were conducted for lichens and bryophytes on trees and rocks, vascular plants on the forest floor, and for deadwood-associated bryophytes and polypore fungi. Although NMDS ordination analyses of the total species composition identified a main axis related to the age of the oldest trees in two of the study areas, variation partitioning analyses showed that age explained a small fraction of variation of the species composition compared to site conditions, logging history, forest structure, and differences between the sites in all habitats. The unique variation explained by forest age species was, however, significant for all habitats. The fraction of variation in species composition explained by forest age was the largest for lichens and bryophytes on trees, and for deadwood-associated bryophytes and polypore fungi. Our results suggest that practical mapping of near-natural forests for management purposes inventories should include site conditions, forest structure and between site differences in addition to forest age.

Pushed waves, trailing edges, and extreme events: Eco-evolutionary dynamics of a geographic range shift in the owl limpet, Lottia gigantea.

As climatic variation re-shapes global biodiversity, understanding eco-evolutionary feedbacks during species range shifts is of increasing importance. Theory on range expansions distinguishes between two different forms: "pulled" and "pushed" waves. Pulled waves occur when the source of the expansion comes from low-density peripheral populations, while pushed waves occur when recruitment to the expanding edge is supplied by high-density populations closer to the species' core. How extreme events shape pushed/pulled wave expansion events, as well as trailing-edge declines/contractions, remains largely unexplored. We examined eco-evolutionary responses of a marine invertebrate (the owl limpet, Lottia gigantea) that increased in abundance during the 2014-2016 marine heatwaves near the poleward edge of its geographic range in the northeastern Pacific. We used whole-genome sequencing from 19 populations across >11 degrees of latitude to characterize genomic variation, gene flow, and demographic histories across the species' range. We estimated present-day dispersal potential and past climatic stability to identify how contemporary and historical seascape features shape genomic characteristics. Consistent with expectations of a pushed wave, we found little genomic differentiation between core and leading-edge populations, and higher genomic diversity at range edges. A large and well-mixed population in the northern edge of the species' range is likely a result of ocean current anomalies increasing larval settlement and high-dispersal potential across biogeographic boundaries. Trailing-edge populations have higher differentiation from core populations, possibly driven by local selection and limited gene flow, as well as high genomic diversity likely as a result of climatic stability during the Last Glacial Maximum. Our findings suggest that extreme events can drive poleward range expansions that carry the adaptive potential of core populations, while also cautioning that trailing-edge extirpations may threaten unique evolutionary variation. This work highlights the importance of understanding how both trailing and leading edges respond to global change and extreme events.

CDT-CAD: Context-Aware Deformable Transformers for End-to-End Chest Abnormality Detection on X-Ray Images.

Deep learning methods have achieved great success in medical image analysis domain. However, most of them suffer from slow convergency and high computing cost, which prevents their further widely usage in practical scenarios. Moreover, it has been proved that exploring and embedding context knowledge in deep network can significantly improve accuracy. To emphasize these tips, we present CDT-CAD, i.e., context-aware deformable transformers for end-to-end chest abnormality detection on X-Ray images. CDT-CAD first constructs an iterative context-aware feature extractor, which not only enlarges receptive fields to encode multi-scale context information via dilated context encoding blocks, but also captures unique and scalable feature variation patterns in wavelet frequency domain via frequency pooling blocks. Afterwards, a deformable transformer detector on the extracted context features is built to accurately classify disease categories and locate regions, where a small set of key points are sampled, thus leading the detector to focus on informative feature subspace and accelerate convergence speed. Through comparative experiments on Vinbig Chest and Chest Det 10 Datasets, CDT-CAD demonstrates its effectiveness in recognizing chest abnormities and outperforms 1.4% and 6.0% than the existing methods in AP50 and AR on VinBig dateset, and 0.9% and 2.1% on Chest Det-10 dataset, respectively.

Towards precision cancer medicine for Aboriginal and Torres Strait Islander cancer health equity.

Delivering cancer control at scale for Aboriginal and Torres Strait Islander communities is a national priority that requires Aboriginal and Torres Strait Islander leadership and codesign, as well as significant involvement of the Aboriginal community-controlled health sector. The unique genomic variation observed among Aboriginal and Torres Strait Islander peoples may have implications for standard and precision medicine. Yet, Aboriginal and Torres Strait Islander peoples are absent from, or under-represented within, human reference genome resources, genomic studies, cancer studies, cancer cell lines, patient-derived xenografts and cancer clinical trials. Genomics-guided precision cancer medicine offers an opportunity to reduce cancer health disparities experienced by Aboriginal and Torres Strait Islander peoples through personalising prevention, diagnosis, treatment and long term management. Here, we describe what is required to ensure that Aboriginal and Torres Strait Islander peoples can receive the benefits of precision cancer medicine. Equity of access to care, an Aboriginal and Torres Strait Islander cancer workforce, and appropriate genome reference resources are important for safe and effective cancer medicine. Building Indigenous data sovereignty principles and Aboriginal and Torres Strait Islander governance into research is required to protect Aboriginal and Torres Strait Islander rights and collective interests. Aboriginal and Torres Strait Islander community engagement should be undertaken to develop an understanding of the unique cultural and ethical considerations for precision cancer research. Local and national genomic health research guidelines are needed to define a consensus best practice in genomics research with Aboriginal and Torres Strait Islander peoples.

Cognate Object dalam Bahasa Jepang: Bentuk dan Contoh

Syntax is concerned with how sentences are constructed. Language users employ a unique variation that allows for the formation of elements in sentences. Subject, verb, object, complement, and adverb are some sentence structure components that catch the attention of syntax. This research will concentrate on objects, particularly cognate objects, among the many sentence structures studied from syntax. Although the Japanese language has a cognate object similar to English, there is a slight difference. This is because the object comes before the predicate in the Japanese sentence pattern. As a result, this paper will look at how cognate object forms in Japanese. Some of the facts in the discussion suggest that the cognate object in Japanese is significantly different from the cognate object in English. This is due to the non-referential and non-accusative nature of the cognate object in Japanese. In Japanese, cognate object forms based on verbs have nearly the same qualities as verbs.

On solvability and optimal controls for impulsive stochastic integrodifferential varying-coefficient model

This article concentrates in analyzing optimal controls for stochastic integrodifferential equation (SIDE) in Hilbert space. Necessary parameters are imposed to demonstrate the system that follows a unique variation of parameter formula using Leray Schauder Alternative. Subsequently, the existence of optimal control is investigated for the considered Lagrange control problem. The theoretical example with the mechanical example of ethanol fuelled engine are discussed to validate the results obtained.

The dominance of dorsal scapular artery as the blood supply to muscles of the back in the absence of two primary vessels: a cadaveric case report

PurposeUnderstanding of rare or unknown anatomical variations of the vasculature of the neck is critical to reduce the risk of complications during surgeries and other invasive procedures in the neck and shoulder regions.MethodsBilateral dissection of the neck and muscles of the back of an 87-year-old Caucasian male donor was performed to demonstrate the origin, course and termination of the arteries that arise in the neck.ResultsSeveral anatomical variations were noted on the right side of the neck of the donor body – (i) only inferior thyroid and ascending cervical arteries originated from the thyrocervical trunk (TCT), from the first part of the subclavian artery (SA), whereas the transverse cervical (TCA) and suprascapular (SSA) arteries were entirely absent, (ii) Dorsal scapular artery (DSA) emerged normally from the third part of the SA. However, after supplying the rhomboids and levator scapulae muscles, DSA provided two additional branches to the trapezius muscle and a branch to the supraspinatus muscle. Interestingly, the branches to the trapezius muscle from the DSA were the only sources of blood supply to the muscle.ConclusionWe report a unique anatomical variation involving the absence of the TCA and SSA from the TCT. The unilateral absence of these major vessels and the branches of DSA supplying the trapezius and supraspinatus muscles have not been reported previously in the literature in a single case report. This case study may provide useful information for head and neck reconstruction and shoulder repair surgeries.

Unconventional Monetary Policy Transmission and Bank Lending Relationships

Firms with only one bank relationship make up the majority of firms in many economies. This paper explores whether policy-driven lending is differentially transmitted to single-bank firms in comparison with the multibank firms that are the focus of the literature. Using unique variation in the ECB’s very long-term refinancing operations (VLTROs), which affected lending to firms discontinuously across credit ratings but within banks, we find selective transmission of VLTRO liquidity to single-bank firms. Banks apply higher lending standards to single-bank firms, with banking relationships determining both new lending and lending maturity. By contrast, banks appear to transmit policy lending near-uniformly across multibank firms. This paper was accepted by David Sraer, finance. Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2022.01871 .

A unique variation of the common peroneal nerve: a case report

This variation was observed in the left lower extremity of a 75-year-old male cadaver fixed with formalin, ethanol and glycerol solution. The common peroneal nerve was divided into six terminal branches. These branches were identified as deep peroneal nerve, superficial peroneal nerve, two muscular branches to the tibialis anterior and two muscular branches to the peroneus longus. Knowledge of the variations of the common peroneal nerve and its branches is important in knee and proximal leg operations.

Variasi Tuturan Basa Walikan dalam Sosial Media: Studi Kasus Komunitas Pemuda-Pemudi di Kota Malang

The use of the walikan language as an expression of pride in identity in the Malang youth community has different variations depending on the speakers. The unique variations of the walikan bases can arise due to regional and gender differences between men and women. This study aims to examine how the unique variations of basa walikan can occur based on differences in social strata and gender using a socio-dialectological approach. Through a socio-dialectological approach, it can be known about the variations in the language used by the Malang youth community in using digital tools. This research uses a descriptive-qualitative approach. The data collection technique uses the listening and taking notes technique which in this case is represented through listening activities and being directly involved in communication using the Walikan language through social media applications, namely Instagram. Another technique used to further deepen the analysis is the interview technique. In addition, the equating comparison technique and the differential comparison technique were also used to analyze the data that had been collected. The results of this study indicate that there is a unique variation of the walikan base used by Malang youth, namely, the walikan base variation in the lexicon order, speech acts, and semantics.

Wartenberg’s syndrome – A unique presentation resulting from compression of the superficial radial nerve from an anomalous radial artery branch

Wartenberg’s syndrome is a compression sensorineural mononeuropathy of the superficial branch of the radial nerve. This case report describes a patient, who was severely debilitated by compression of the superficial branch of the radial nerve that she was unable to work. The case highlights a unique anatomical variation in the course of the deep terminal branch of the superficial radial artery causing compression of the superficial branch of the radial nerve. To the best of our knowledge, this has never been described before and should be considered a rare cause of Wartenberg’s syndrome. Surgery can provide both diagnostic and therapeutic benefits and should be considered early in such cases, especially when investigations are contradictory.