Unique Fatty Acid Profile Research Articles

Fatty acid unsaturation improves germination of upland cotton (Gossypium hirsutum) under cold stress.

The level of fatty acid unsaturation in seeds is one of the major determinants of cold germination ability, particularly in oilseeds. The presence of cis double bonds in unsaturated fatty acids creates bends that lowers their melting temperatures compared to saturated fatty acids. Unsaturated fatty acids with low melting points mobilize faster at low temperatures providing seeds with sufficient energy for germination. To investigate the effects of fatty acid unsaturation on the ability of cotton seeds to germinate under cold conditions, four recombinant inbred lines (RILs) of cotton with unique fatty acid profiles were evaluated using a set of developmental and biochemical assays at 12°C (critically low temperature), 15°C (cardinal minimum temperature) and 30°C (optimum temperature). Furthermore, whole seed lipidome profiling using liquid chromatography with mass spectrometry was done to compare the lipid compositional changes at 12°C and 30°C after imbibing cotton seeds of all the six genotypes for 0 hours, 3 hours and 6 hours. The RILs with higher unsaturation/saturation ratios registered robust germination performance, lower solute leakage, and optimum water uptake rates under cold stress. Imbibition at 30°C for 8 hours before cold exposure significantly improved the germination of cold sensitive genotypes, indicating that the first few hours of water uptake are critical for cold stress. Whole seed lipidome profiling of all the genotypes specifically associated cold germination ability with higher unsaturation levels of phospholipids during early imbibition. The presence of cis double bonds in phospholipids creates kinks that maintain the fluidity of cell membranes under low temperature. Membrane flexibility under cold conditions is essential for facilitating key germination events including membrane organization and respiration. The current results highlight the importance of fatty acid composition in cold germination ability of upland cotton.

Utilization of mango seed oil as a cocoa butter replacer for the development of innovative chocolate

ABSTRACT Chocolate is the most popular food type and flavor in this world. The key ingredient in many chocolate products is cocoa butter due to its unique fatty acid profile. Due to the expensive nature of cocoa butter, it has stimulated extensive research for fats that are cheaper and more easily available and can be used as cocoa butter substitutes. The mango (Mangifera indica) is known as the king of fruit due to its rich nutritive profile. The major parts of mango fruit are peel, pulp, and seed. Mango seed is usually discarded as waste which is a source of edible oil (7–12%). The current study was designed to produce chocolate with mango seed oil as a cocoa butter replacer. Mango seed oil was extracted using the soxhlet apparatus and its physiochemical properties were evaluated. Extracted oil was used in chocolate preparation with different proportions (0%, 30%, 70%, and 100%). Furthermore, chocolate was subjected to explore the effect of storage (21days) on product quality and sensory with 7days interval. Current results show that mango seed oil has a valuable fat profile containing palmitic acid (C16:0) 26%, stearic acid (C18:0) 36%, and oleic acid (C18:2) 33%. Moreover, innovative chocolate showed higher antioxidant activity as compared to control in different storage intervals. In addition, chocolate prepared with different proportions of mango seed oil showed higher sensory scores as compared to the control sample. The findings suggest that mango seed oil can replace cocoa butter in chocolate and reduce/manage mango seed waste to improve its antioxidant activity and nutritional value.

Dietary Inclusion of Yellow Mealworms (T. molitor) and Lesser Mealworms (A. diaperinus) Modifies Intestinal Microbiota Populations of Diet-Induced Obesity Mice

BackgroundInsect-based proteins are high-quality alternatives to support the shift toward more sustainable and healthy diets. Additionally, insects contain chitin and have unique fatty acid profiles. Studies have shown that mealworms may beneficially affect metabolism, but limited information is known regarding their effects on gut microbiota. ObjectivesWe determined the effects of defatted yellow mealworm (Tenebrio molitor) and whole lesser mealworm (Alphitobius diaperinus) meals on the intestinal microbiota of diet-induced obesity mice. MethodsMale C57BL/6J mice were fed a high-fat diet (HFD; 46% kcal) to induce obesity. Obese mice were then randomly assigned to treatments (n = 10/group) and fed for 8 wk: HFD, HFD with casein protein; B50, HFD with 50% protein from whole lesser mealworm; B100, HFD with 100% protein from whole lesser mealworm; Y50, HFD with 50% protein from defatted yellow mealworm; Y100, HFD with 100% protein from defatted yellow mealworm. Lean mice (n = 10) fed a low-fat-diet (10% kcal) were included. Fresh feces were collected at baseline and every 2 wk, with cecal digesta collected at kill. Fecal and cecal DNA was analyzed for microbiota using 16S rRNA MiSeq Illumina sequencing. ResultsIn feces and cecal digesta, mice fed mealworms had greater (P < 0.05) bacterial alpha diversity, with changes occurring in a time-dependent manner (P < 0.05). Beta diversity analyses of cecal samples showed a clear separation of treatments, with a time-based separation shown in fecal samples. Widespread microbial differences were observed, with over 45 genera altered (P < 0.05) by diet in cecal digesta. In feces, over 50 genera and 40 genera were altered (P < 0.05) by diet and time, respectively. ConclusionMealworm consumption changes the intestinal microbiota of obese mice, increasing alpha diversity measures and shifting bacterial taxa. More investigation is required to determine what mealworm components are responsible and how they may be linked with the metabolic benefits observed in mealworm-fed mice.

Optimization of Hazelnut Spread Based on Total or Partial Substitution of Palm Oil.

Palm oil is widely used in the manufacturing of hazelnut-based spreads due to its unique fatty acid and triacylglycerol profile and, thus, its crystallization behaviour, which makes it suitable for use in fat-based spreadable products. An interesting solution that enables the replacement of palm oil is given by oleogels made with high nutritional quality oil. In this study, the influence of the replacement of palm oil with different glycerol monostearate/olive oil-based oleogels, as well as the influence of the different amounts of GMS employed in oleogel preparation, on the oil binding capacity, spreadability, and rheological and sensory parameters of hazelnut cocoa spreads was investigated. A design of experiment (DoE) approach, with the adoption of the D-optimal design, was used to plan the cocoa hazelnut spread formulations, with the aim being to identify the optimal formulation with desirable quality parameters in terms of Casson's viscosity, spreadability, and oil binding capacity. The resulting optimized formulation was identified in a spread characterized by a total replacement of palm oil with an oleogel made of 95% olive oil and 5% GMS.

Antioxidant Enzyme System Modulation by Dietary Palm Oils, Palm Kernel Oil and Soybean Oil in Laying Hens.

Palm-based oils (palm oil and kernel oil) and soybean oil have unique fatty acid and antioxidant profiles based on the compounds present in them. Hence, this study elucidated the antioxidant properties of crude palm oil (CPO), red palm oil (RPO), refined palm oil (RBD), palm kernel oil (PKO) and soybean oil (SBO) and the influence of dietary oils on blood lipid profiles, tissue fatty acid deposition and the expression of hepatic lipid and lipoprotein metabolism genes in laying hens. The oils were analyzed for color, beta-carotene, free fatty acid and acid value, phenolic content and lipid peroxidation. In an in vivo trial, 150 laying hens were allotted into five groups and supplemented with either CPO, RPO, RBD, PKO or SBO for 16 weeks. High antioxidant compounds present in palm oils help reduce the oxidation of oils. Dietary supplementation with palm oils, particularly CPO and RPO, contributed to the lower liver, serum and jejunal mucosal antioxidant enzyme activities. The antioxidant enzyme genes in the jejunal mucosa were downregulated in palm oils and PKO, but there was no difference between oils in antioxidant enzyme genes in the liver. In conclusion, dietary supplementation with oils with high antioxidant content contributed to protection against oxidation and was associated with a lower requirement for producing antioxidant enzymes.

Nuts and berries from agroforestry systems in temperate regions can form the foundation for a healthier human diet and improved outcomes from diet-related diseases.

Agroforestry is a specific type of agroecosystem that includes trees and shrubs with the potential to yield nutrient-rich products that contribute to human health. This paper reviews the literature on the human health benefits of tree nut and berry species commonly associated with agroforestry systems of the United States, considering their potential for preventing certain diet-related diseases. Emphasis is placed on those diseases that are most closely associated with poor outcomes from COVID-19, as they are indicators of confounding health prognoses. Results indicate that tree nuts reduce the risk of coronary heart disease, and walnuts (Juglans species) are particularly effective because of their unique fatty acid profile. Berries that are grown on shrubs have the potential to contribute to mitigation of hypertension, prevention of Type II diabetes, and reduced risk of cardiovascular disease. To optimize human health benefits, plant breeding programs can focus on the traits that enhance the naturally-occurring phytochemicals, through biofortification. Value-added processing techniques should be selected and employed to preserve the phytonutrients, so they are maintained through the point of consumption. Agroforestry systems can offer valuable human health outcomes for common diet-related diseases, in addition to providing many environmental benefits, particularly if they are purposefully designed with that goal in mind. The food system policies in the U.S. might be reoriented to prioritize these food production systems based on the health benefits.

53 Hemp seed oil as a novel fatty acid supplement for horses

Polyunsaturated fatty acids (PUFA) play a role in regulating the body's response to inflammation. Most grains fed to horses are high in linoleic acid (LA), a pro-inflammatory n6 PUFA, compared with α-linolenic acid (ALA), an anti-inflammatory n3 PUFA. Recent interest in hemp (Cannabis sativa) seed oil (HSO) as a PUFA source has arisen due to its unique fatty acid (FA) profile, which includes γ-linolenic acid (GLA), an n6 PUFA with anti-inflammatory properties. Dietary GLA is rapidly converted to dihomo-γ-linolenic acid (DGLA), a precursor to anti-inflammatory eicosanoids. Manipulating dietary FA may lead to alterations in tissue FA profiles in the horse which could promote a reduced inflammatory response. Thus, our objective was to determine if oral supplementation of HSO for 28 d would cause detectable changes in FA composition in synovial fluid (SF) and skeletal muscle (MUS). Six Thoroughbred geldings (11 ± 3.2 yrs, 568 ± 26 kg BW) were used in a crossover experiment with 2 consecutive 63d periods. Horses were offered a control (CON) basal diet of hay and concentrate or the basal diet with the addition of 166 mL HSO delivering 5g GLA. Diets were designed to be isocaloric. Over 7d, HSO was introduced gradually, maintained for another 28d, and then removed and horses resumed basal diets for an additional 28d. Horses were weighed, and body condition score (BCS) assessed weekly. MUS and SF samples werecollected on d0, d35, and d63 of each period. MUS biopsies were taken from the middle gluteus muscle at a depth of 8cm. SF was collected from the left carpus joint. FA were extracted from MUS and SF and analyzed by GC. Individual FA are represented as g of FA per 100g of total fatty acids. Data were analyzed using Proc MIXED in SAS (v.15.1 SAS Institute Inc., Cary, NC). No changes in BW or BCS were observed throughout the study. In SF, GLA was detected after 28d of HSO supplementation (0.32 ± 0.06g/100g) but not at any other time point. On d 28, horses supplemented with HSO also had greater SF DGLA (0.31 ± 0.04g/100g) than CON (0.19 ± 0.04g/100g; P = 0.04). MUS ALA tended (P = 0.07) to be greater in horses fed CON compared with HSO. No other differences were observed for FA in MUS or SF. These results indicate that 28d of HSO supplementation can modify FA profiles in equine skeletal muscle and synovial fluid. This could potentially influence inflammation signaling molecules and subsequently the inflammatory response, however, longer studies with more horses are needed to determine peak incorporation and the effects of varying quantities of HSO in the diet.

Extraction, characterization, and application of tomato seed oil in the food industry: An updated review

Tomato seeds are the by-product of the tomato processing industry used to extract valuable compounds like tomato seed oil (TSO). The TSO is well known for its functional and health-promoting properties. Therefore, recent studies which focused on application of TSO became popular among food manufacturers. This review presents comprehensive methods of TSO extraction and its unique fatty acid profile. The characterization of TSO is briefly discussed, creating a better utilization for various diseases. This review also focused on the multiple application of TSO in the food industry, from dietary supplements to food packaging films, the demand for TSO in the market to improve economic value, and the importance of TSO in the food and medical industry. The TSO can be extensively applicable as a functional compound in the food and bakery industry as a covering material in tomato-based products like tomato sauce and slices. It also acts as a better antioxidant and has antimicrobial properties due to its essential fatty acids like oleic and linoleic acid. The assessment of this article provides an overview of the importance of TSO in food application, from extraction techniques to dietary supplements and its potential for bioactive compounds.

Physicochemical Properties and Sensory Attributes of Cold-Pressed Camelina Oils from the Polish Retail Market

Cold-pressed camelina oil (CPCO) is exceptional seed oil with a unique fatty acid profile promoting health and wellness. Therefore, this work focused on estimating and comparing the physicochemical properties and sensory quality of eight CPCO samples available on the Polish market. All analyzed oils were rich in α-linolenic acid (ALA = 29.91–36.27%) and contained low amounts of saturated fatty acids (SAFA = 10.61–12.20%). Oxidative stability of the studied CPCO samples, using the Rancimat test, ranged between 4.8 and 6.8 h, while peroxide (PV = 0.58–4.61 meq O2/kg) and anisidine (AnV = 0.15–1.60) values differed significantly. Moreover, the water and volatile matter contents (WVMC = 0.05–0.17%) and phosphorus level (P = 3.03–13.58 mg/kg) were monitored in commercial CPCO samples. Low concentrations of polycyclic aromatic hydrocarbon contaminants (Σ4PAHs = 0.72–7.22 μg/kg) were established in all oils. A quantitative descriptive analysis (QDA) was developed to characterize the sensory properties of eight CPCO samples. Six oil samples had high overall sensory quality (OSQ &gt; 4.0), but OSQ &lt; 3.5 was an unacceptance sensory quality for two of the oils. The developed lexicon might be used in the oil industry to monitor product quality, sensory profiling of new product development, and benchmark competitors’ samples.

Seed and Fruit Fatty Acid Compositions of Crambe orientalis and Crambe tataria Oils Collected from Three Different Provinces in Turkey

Crambe is an annual or perennial plant belonging to the Brassicaceae family. The interest in Crambe is mainly due to its unique fatty acid profile, low input management, and broad adaptability to the environment. Thus, Crambe seed and fruit fatty acids were investigated by GC / MS in order to examine the chemical variability which correlates with the environmental variability. In the study, Crambe orientalis var. orientalis and Crambe tataria var. tataria were used and they were collected from the different areas of three provinces of Türkiye. Fifteen compounds were found, representing 84.6-100% of the total seed and fruit oils. The major components in both species were erucic acid (24.7-44.7%), gondoic acid (19.0-28.3%), oleic acid (14.9-28.5%), and linoleic acid (8.1-17.6%). As a consequence, it is needed to increase the production of erucic acid (C22:1) and gondoic acid (C20:1) in Crambe by using wild populations via plant breeding. These two promising fatty acids may be an alternative to meet industrial fatty acid market demand in a more environmentally friendly way compared to using fatty acids from fossil oil.

Optimization of a Calcium-Based Treatment Method for Jellyfish to Design Food for the Future.

Edible jellyfish are a traditional Southeast Asian food, usually prepared as a rehydrated product using a salt and alum mixture, whereas they are uncommon in Western Countries and considered as a novel food in Europe. Here, a recently developed, new approach for jellyfish processing and stabilization with calcium salt brining was upgraded by modifying the pre-treatment step of freshly caught jellyfish and successfully applied to several edible species. Treated jellyfish obtained by the application of the optimized version of this method respected both quality and safety parameters set by EU law, including no pathogenic microorganisms, absence or negligible levels of histamine and of total volatile basic nitrogen, no heavy metals; and the total bacterial, yeast, and mold counts were either negligible or undetectable. Jellyfish treated by the presented method exhibited unique protein content, amino acid and fatty acid profiles, antioxidant activity, and texture. The optimized method, initially set up on Rhiszostoma pulmo, was also successfully applied to other edible jellyfish species (such as Cotylorhiza tuberculata, Phyllorhiza punctata, and Rhopilema nomadica) present in the Mediterranean Sea. This study discloses an innovative process for the preparation of jellyfish-based food products for potential future distribution in Europe.

Metabolic routing maintains the unique fatty acid composition of phosphoinositides.

Phosphoinositide lipids (PPIn) are enriched in stearic- and arachidonic acids (38:4) but how this enrichment is established and maintained during phospholipase C (PLC) activation is unknown. Here we show that the metabolic fate of newly synthesized phosphatidic acid (PA), the lipid precursor of phosphatidylinositol (PI), is influenced by the fatty acyl-CoA used with preferential routing of the arachidonoyl-enriched species toward PI synthesis. Furthermore, during agonist stimulation the unsaturated forms of PI(4,5P)2 are replenished significantly faster than the more saturated ones, suggesting a favored recycling of the unsaturated forms of the PLC-generated hydrolytic products. Cytidine diphosphate diacylglycerol synthase 2 (CDS2) but not CDS1 was found to contribute to increased PI resynthesis during PLC activation. Lastly, while the lipid transfer protein, Nir2 is found to contribute to rapid PPIn resynthesis during PLC activation, the faster re-synthesis of the 38:4 species does not depend on Nir2. Therefore, the fatty acid side-chain composition of the lipid precursors used for PI synthesis is an important determinant of their metabolic fates, which also contributes to the maintenance of the unique fatty acid profile of PPIn lipids.

Understanding the beneficial effects of using designer lipids in the formulation of cookies

The designer lipids (DL) synthesized using enzymatic acidolysis was used as a potential substitute for palm oil shortening (POS) in cookies. DL showed higher oxidative stability than POS and there was no trans-fat found in the case of DL, offering advantage as compared to significant trans-fat in POS. The unique fatty acid profile of DL containing about 62.9 ± 0.1% caprylic acid and 28.3 ± 0.0% linolenic acid enhanced the nutritional properties of DL and thus the cookies. The DL-based cookies showed higher spread ratio, higher set time, and quick spreadability which are highly desirable. Due to lower aeration properties of DL, the cookies were harder than POS-based cookies. The overall acceptability in terms of sensory attributes evaluated for 28 days was higher for DL-based cookies. The DL proved to be a potential healthy replacement for commonly applied bakery POS having various health issues. Novelty impact statement The work presents a novel approach of using designer lipids as a potential replacement to palm oil shortening with an objective to improve the quality and healthy nature. Detailed evaluation of different textural, physical, and sensory properties as well as oxidative stability has been presented so as to elucidate the beneficial application of designer lipids and associated value addition. The work is expected to be of much commercial relevance in the food and nutrition sector.

Growth Performance, Antioxidant Activity, Immune Status, Meat Quality, Liver Fat Content, and Liver Histomorphology of Broiler Chickens Fed Rice Bran Oil.

Simple SummaryThere are numerous approaches for enrichment of broiler’s meat with valuable nutrients, for instance the enrichment with polyunsaturated fatty acids (PUFA). The addition of vegetable oils in the diets of broilers is an appropriate strategy to enrich the chicken meat with beneficial FA, however, this enrichment is accompanied by a lipid peroxidation with a resultant decrease in the nutritional value, quality, and shelf-life of the meat, and for that reason, the dietary supplementation with antioxidants becomes necessary. What places rice bran oil (RBO) on top of other vegetable oils is its antioxidant components and unique fatty acid profile and it is reported to induce substantial lipid-reducing effects and antioxidant properties. Therefore, this study was performed to determine the influence of RBO inclusion in the diets of broiler chickens on performance, carcass characteristics, blood parameters, meat quality, antioxidant activity, liver lipid content, and liver histological structure. RBO inclusion had a positive effect on the growing performance, dressing percentage, and immune status. Furthermore, RBO supplementation decreased the abdominal fat yield and EE content in the meat, while it increased the content of PUFA in the meat, which may be beneficial for consumers. RBO improved the antioxidant capacity of the meat and the liver, whereas it reduced the concentration of cholesterol and triglycerides in the blood, meat and liver. RBO could be used as an efficient ingredient in broiler chickens’ diets to improve performance, immune status, antioxidant activity, blood lipid profile, and the nutritive value of meat.This trial was performed to determine the effect of rice bran oil (RBO) inclusion in diets of broiler chickens on performance, carcass characteristics, blood parameters, meat quality, antioxidant activity, liver lipid content, and liver histological structure. The 35-day feeding trial was conducted on 240 one-day-old Ross 308 broiler chickens, allocated to four treatment groups with six replicates each. RBO was examined at different inclusion levels, 0% (control), 1% (RBO1%), 1.5% (RBO1.5%), and 2% (RBO2%) in a completely randomized design. The results showed that at the end of the trial (35 days) the RBO supplementation had positive effects (p < 0.001) on the productivity parameters, but the feed intake was linearly decreased due to RBO inclusion. In addition, RBO supplementation linearly improved (p < 0.05) the dressing percentage, breast yield, immune organs relative weights, and meat glutathione concentration, while it decreased (p < 0.01) the abdominal fat yield and meat crude fat, triglycerides, cholesterol, and Malondialdehyde (MDA) contents in broiler’s meat. Moreover, serum total protein, globulin, and high-density lipoprotein contents improved noticeably (p < 0.01) due to offering an RBO-supplemented diet, but serum total lipids, total cholesterol, triglyceride, low-density lipoprotein, and aspartate aminotransferase concentrations linearly reduced (p < 0.01). The RBO supplementation augmented (p < 0.05) the phagocytic index, phagocytic activity, and antibody titer compared to control. On the other hand, RBO inclusion had no effect on the breast, thigh, or abdominal fat color parameters. Moreover, RBO supplementation reduced (p < 0.01) the content of total saturated FA (SFA), but increased (p < 0.01) the content of total monounsaturated FA (MUFA), and polyunsaturated FA in both breast and thigh meat. Chemical analysis of the liver tissue samples revealed that the inclusion of RBO linearly reduced (p < 0.05) hepatic cholesterol, triglyceride, and MDA contents. Histologically, the lipid percentage and number of lipid droplets (p < 0.01) were markedly lessened in the RBO-supplemented groups. The histological structure of the liver asses by light and electron microscope were normal in all groups without any pathological lesions. It is concluded that RBO could be used as a valuable ingredient in broiler chickens’ diets to stimulate the growing performance and immune status, enhance the antioxidant activity and blood lipid profile, augment liver function, and improve the nutritive value of the meat.

Unique Trans-fatty Acid Profile in Children With Attention Deficit Hyperactivity Disorder.

Background: Attention deficit hyperactivity disorder (ADHD) is the most common developmental disorder in children. Studies suggest an association between fatty acids composition and ADHD pathogenesis. We aimed to investigate whether children diagnosed with ADHD present unique fatty acid profiles in red blood cells (RBC), as compared to children without ADHD.Method: We examined 60 children aged 6–14 years, out of which 32 were diagnosed with ADHD, and 28 were not. Blood was collected from all children to quantify an array of 26 fatty acids from RBC membranes. Fatty acid methyl esters were generated by acid transesterification and analyzed by gas chromatography.Results: We found that children with ADHD presented unique fatty acid profiles on RBC membranes with significantly higher levels of most of the trans-fatty acids (Total trans-fatty acids 0.64 ± 0.21 vs. 0.49 ± 0.18 p = 0.003) and lower levels of docosahexaenoic acid (DHA), as compared to controls (4.06 ± 0.79 vs. 4.68 ± 1.37 p = 0.040). Additionally, total trans-fatty acids were higher in children with extremely severe clinical ADHD condition score, as compared to milder ADHD scores and to control children (0.72 ± 0.18, 0.64 ± 0.20, 0.61 ± 0.22, 0.49 ± 0.18, p = 0.010, accordingly).Conclusion: Children with ADHD have higher levels of trans-fatty acids in RBCs, compared to children without ADHD. This study points to a possible link between trans-fatty acids and ADHD. Understanding these findings and the clinical meaning will potentially contribute to a more targeted dietary intervention.

The identification of fish oils in 20th century paints and paintings

• Historic recipes show 19th-20th c. house paint manufacturers in the USA used fish oil. • Fish oil was cheaper than linseed oil and its presence in paints was not advertised. • GC–MS or HPLC-MS identify the unique fatty acid or TAGs profile of fish oil in paints. • Fish oil mixtures in some historic artists’ paints suggest adulteration. • Fish oil containing paints have been found in paintings by well-known artists. Vegetable oils such as linseed oil are commonly used in retail trade and artists’ oil and alkyd paints, but examination of historic trade literature from the 19 th and 20 th centuries suggests that paint manufacturers in the USA also used fish oils, especially from menhaden ( Brevoortia sp.). Gas chromatography-mass spectrometry and high-performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry of menhaden oil samples provided by Werner G Smith Inc., a modern company that supplies menhaden oil to the coatings industry, reveal a fatty acid profile distinct from that of vegetable oils. Menhaden oil has high levels of myristic acid, low levels of lauric acid and a high palmitic to stearic acid ratio and so is readily distinguished from vegetable oils by either of these methods. Historic paint recipes indicate that menhaden oils were often mixed with vegetable oils, so to establish detection limits we analyzed combinations of menhaden oil and either linseed, safflower or sunflower oils by GC–MS; the high levels of myristic acid remain characteristic in mixtures containing only 20% menhaden oil. A selection of historic artists’ paints pre-dating 1970 and paint samples taken from paintings dating from the 1950s and 1960s were analyzed to determine if fish oils could be detected. Fish oils may be present in historic artists’ paints, including those made by the Bocour Company, and in paintings by American artists Franz Kline, Barnett Newman, David Smith, and Andy Warhol. This is the first positive identification of fish oils in historic artists’ paints and 20 th century artworks.

Comprehensive review on sea buckthorn: Biological activity and its potential uses

This review is result of various research and studies of comprehensive material regarding nutritional composition, biological activities as well as uses of sea buckthorn as food, medicine, cosmeceuticals, and feed. Sea buckthorn holds unique nutritional composition with Vitamins (A, C, D, E, F, K, P, and B complex vitamins), 18 free amino acid and unique unsaturated fatty acid profile that makes berry only plant source of omega- 7 fatty acid. Its bioactive phytochemical compounds possess various biological activity such as antioxidant activity, immunomodulatory, anti-carcinogenic, hepatoprotective, cardioprotective, anti-atherogenic and radioprotective etc. in nature. Sea buckthorn is primarily consumed in its natural form although various products has been prepared. The potential of sea buckthorn as nutraceutical is abundant. When it comes to its medicinal aspect it can be used as extracts of pure form for curative as well as preventive measure of medical conditions. Many studies have showed Sea buckthorn as effective game changer to treat cancer as well as cardiovascular disorders and gastrointestinal ulcers. Cosmeceuticals that use sea buckthorn as ingredients has found to be effective on blackheads, dry skin, etc. Also, it is used as medicine in various skin diseases. Along with all the benefits and potentials it is necessary to develop effective products i.e., food, drugs, feed, cosmeceuticals in a market suitable and commercializable way to achieve and justify consumer demand and market trend.

Effect of the growing area on the fat content and the fatty acid composition of Ecuadorian cocoa beans

Cocoa presents a high fat content and a unique fatty acid profile defining its technological and nutritional properties. This study evaluated the fat content and fatty acid composition of Nacional cocoas, a worldwide recognised “fine” variety, collected in 85 Ecuadorian farms while taking into account 3 geographical levels (region, province, and canton). The total fat content varied from 45.61 ± 1.27 to 52.13 ± 0.58 g/100 g DW and was higher in the provinces and cantons from the Amazonian region than in those from the Pacific Coast region. A remarkable effect of the region and the province was shown on the content of individual fatty acids of Nacional cocoa beans. Total amounts of saturated and unsaturated fatty acids also depended on the growing area. Multivariate analysis provided a comprehensive assessment of the cocoa fat composition according to the origin, which may be useful for the selection of cocoas with specific technological and nutritional characteristics.

Omega-3 polyunsaturated fatty acids modify the inverse association between systemic inflammation and cardiovascular fitness

Exercise increases quality of life and lowers all-cause mortality, likely by preventing cardiovascular disease. Although the beneficial effects of exercise are linked with reductions in chronic inflammation, individual responses vary and factors that contribute to the anti-inflammatory effects of cardiovascular fitness remain largely undefined. We sought to investigate the role of fatty acids in the inverse relationship between inflammation and cardiovascular fitness. In this cross-sectional study using data from 435 participants in NHANES and linear regression models with CRP as the outcome, we observed significant negative interactions between VO2max and omega-3 polyunsaturated fatty acids (PUFAs) but not saturated, monounsaturated, or omega-6 PUFAs. When stratified by omega-3 PUFA tertiles, participants in the medium tertile, but not low tertile, show an enhanced negative association between VO2max and CRP, with a-32.0% difference (95% CI:-44.95,-15.9%) per 10mL/kg/min of VO2max. Exploratory factor analysis identified five unique dietary fatty acid (FA) profiles. The FA profile consisting predominantly of omega-3 PUFA had the strongest negative association for VO2max and CRP, with a-28.2% difference in CRP (95% CI:-43.4,-8.9) per 10mL/kg/min of VO2max. We also found that alpha-linolenic acid (ALA) and docosahexaenoic acid (DHA) enhanced the negative association between VO2max and CRP, suggesting that the anti-inflammatory response to VO2max capacity is associated with ALA and DHA levels. Males, Whites, and individuals with lower BMI were more sensitive to the effects of omega-3 PUFAs, while having high SFA levels attenuated the benefit. This study suggests that omega-3 PUFAs are effect modifiers for VO2max and CRP and that the anti-inflammatory benefits of increasing cardiovascular fitness are associated with omega-3 PUFAs.

Parenteral lipid emulsions induce unique ileal fatty acid and metabolomic profiles but do not increase the risk of necrotizing enterocolitis in preterm pigs

Exposure to parenteral lipid emulsions induces unique intestinal fatty acid and metabolomic profiles; however, these profiles are not linked to a difference in NEC risk in preterm pigs. Metabolomic and proteomic analyses provide mechanistic insights into NEC pathogenesis. Compared with healthy ileal tissue, metabolites in tryptophan metabolism and arachidonic acid-containing glycerophospholipids are increased in NEC tissue. Proteomic analysis differentiates NEC versus no NEC in the domains of tissue injury, glucose uptake, and chemokine signaling.