<h3>Objective:</h3> To analyze the neuropsychiatric profiles between Amnestic versus Nonamnestic subtypes of mild cognitive impairment. <h3>Background:</h3> Mild cognitive impairment (MCI) reflects cognitive deficits without significant functional decline in activities of daily living. MCI subtypes are based on phenotypic patterns of impairment in cognitive domains (memory, attention, language, executive, visuospatial) as follows: Amnestic single domain (ASD) or multi-domain (AMD), Nonamestic single domain (NASD), or multi-domain (NAMD). Previous studies have suggested Nonamnestic subtypes demonstrate more neuropsychiatric symptoms, but conclusions have been limited by small sample sizes. <h3>Design/Methods:</h3> Using the National Alzheimer’s Coordinating Center (NACC) database (National Institute’s of Aging grant U24 AG072122), we selected all initial Uniform Data Set assessments diagnosed as MCI with subtyping. The NACC data was collected from 37 participating Alzheimer’s Disease Research Centers. Baseline demographic and clinical features, Geriatric Depression Scale (GDS), and Neuropsychiatric Inventory (NPI-Q) were collected. Chi-square analyses were used to compare NPI-Q symptoms and severity across MCI subtypes. <h3>Results:</h3> <h3>Participants:</h3> 9852 individuals with MCI were analyzed: 45.4% AMD, 33.7% ASD, 12.6% NASD, 8.2% NAMD. On NPI-Q, hallucinations, depression, anxiety, apathy, disinhibition, motor disturbances, nighttime behaviors, and appetite changes were significantly more common in Nonamnestic MCI compared to other subtypes (p<.001 for all domains except agitation and irritability [p<.05]). NPI-Q severity scores were significantly higher in Nonamnestic subtypes for depression, apathy, irritability and nighttime behaviors (all p<.01). NAMD was most frequent for all neuropsychiatric symptoms except irritability, wherein NASD had a higher prevalence. NAMD and NASD severity scores were equal for nighttime behaviors and irritability but with higher severity for apathy in NASD. GDS scores were highest in NAMD participants. <h3>Conclusions:</h3> Nonamnestic MCI subtypes are characterized by greater frequency and severity of neuropsychiatric symptoms compared to Amnestic MCI subtypes. <b>Disclosure:</b> Dr. Malone has nothing to disclose. Dr. Elkasaby has nothing to disclose. Dr. Appleby has received personal compensation in the range of $500-$4,999 for serving as a Consultant for Acadia. Dr. Appleby has received personal compensation in the range of $10,000-$49,999 for serving as a Consultant for Ionis. Dr. Appleby has received personal compensation in the range of $0-$499 for serving as a Consultant for Sangamo. The institution of Dr. Appleby has received research support from CJD Foundation. The institution of Dr. Appleby has received research support from Ionis. The institution of Dr. Appleby has received research support from Alector. The institution of Dr. Appleby has received research support from CDC. The institution of Dr. Appleby has received research support from NIH. Dr. Appleby has received publishing royalties from a publication relating to health care. Dr. Appleby has received publishing royalties from a publication relating to health care. Dr. Duffy has nothing to disclose. Dr. Miller Scott has nothing to disclose. Tamara Murphy has nothing to disclose. Paula K. Ogrocki has nothing to disclose. The institution of Dr. Lerner has received research support from Premier applied biosciences. Dr. Lerner has received publishing royalties from a publication relating to health care.